Information on EC 3.4.24.18 - meprin A

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The expected taxonomic range for this enzyme is: Euteleostomi

EC NUMBER
COMMENTARY hide
3.4.24.18
-
RECOMMENDED NAME
GeneOntology No.
meprin A
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of protein and peptide substrates preferentially on carboxyl side of hydrophobic residues
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of amide bond
-
-
hydrolysis of arylamide bond
-
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-
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
148938-24-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
Mus musculus BALB/c
-
-
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Manually annotated by BRENDA team
Mus musculus C57BL/6
-
-
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Manually annotated by BRENDA team
Mus musculus C57BL/6N
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-
-
Manually annotated by BRENDA team
Mus musculus C57BL6
-
-
-
Manually annotated by BRENDA team
Mus musculus male ICR
male ICR
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
-
homology modeling of the protease domain of meprin alpha on the astacin crystal structure and molecular dynamics simulation study, overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(7-methoxycoumarin-4-yl)acetyl-YVADAPK-(K-epsilon-DNP) + H2O
?
show the reaction diagram
-
-
-
-
?
2-aminobenzoic acid-RPPGFSPFRK(2,4-dinitrophenyl)G-OH + H2O
?
show the reaction diagram
-
-
-
?
2-aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Gly-Pro-Phe + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
2-aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Hyp-Gly-Phe + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
2-aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Pro-Gly-Ala + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
2-aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Pro-Gly-Glu + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
2-aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Pro-Gly-Leu + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
2-aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Pro-Gly + Lys-Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
2-aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Arg-Pro-Ile-Phe + Ser-Pro-(4-nitro)Phe-Arg
show the reaction diagram
2-aminobenzoyl-RPPGFSPFRK-(dinitrophenyl)-G + H2O
?
show the reaction diagram
-
fluorogenic bradykinin analog substrate
-
?
actin + H2O
?
show the reaction diagram
-
murine homomeric recombinant enzyme
-
-
?
Aldolase + H2O
?
show the reaction diagram
-
-
-
-
-
alpha-melanocyte stimulating hormone + H2O
?
show the reaction diagram
alpha-melanocyte-stimulating hormone + H2O
?
show the reaction diagram
alpha-MSH + H2O
?
show the reaction diagram
Angiotensin I + H2O
?
show the reaction diagram
Angiotensin I + H2O
Asp-Arg-Val-Tyr + Ile-His-Pro-Phe + Ile-His-Pro-Phe-His-Leu
show the reaction diagram
Angiotensin II + H2O
?
show the reaction diagram
Angiotensin II + H2O
Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
show the reaction diagram
Angiotensin III + H2O
Arg-Val-Tyr + Ile-His-Pro-Phe
show the reaction diagram
annexin A1 + H2O
?
show the reaction diagram
-
substrate identified by 2D IEF/SDS-PAGE-based image analysis
-
-
?
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-(4-nitro)Phe + Ala-Pro-Phe-Arg
show the reaction diagram
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-(4-nitro)Phe + Arg-Pro-Phe-Arg
show the reaction diagram
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-(4-nitro)Phe + Lys-Pro-Phe-Arg
show the reaction diagram
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-(4-nitro)Phe + Phe-Pro-Phe-Arg
show the reaction diagram
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-(4-nitro)Phe + Ser-Pro-Phe-Arg
show the reaction diagram
azocasein + H2O
?
show the reaction diagram
azocasein + H2O
fragments of azocasein
show the reaction diagram
Azocoll + H2O
?
show the reaction diagram
-
poor substrate
-
-
-
B-type natriuretic peptide + H2O
?
show the reaction diagram
B-type natriuretic peptide 1-32 + H2O
B-type natriuretic peptide 8-32 + Ser-Pro-Lys-Met-Val-Gln-Gly
show the reaction diagram
-
-
-
-
?
benzoyl-Gly-His-Leu + H2O
?
show the reaction diagram
benzoyl-L-tyrosyl-4-aminobenzoic acid + H2O
?
show the reaction diagram
Benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Benzyloxycarbonyl-Glu-Lys-Lys 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
poor substrate, hydrolysis at 9.1% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
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Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Benzyloxycarbonyl-Val-Leu-Lys 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
poor substrate, hydrolysis at 10.4% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
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-
Big endothelin I + H2O
?
show the reaction diagram
-
rat
-
-
-
bombesin + H2O
?
show the reaction diagram
Bradykinin + H2O
?
show the reaction diagram
bradykinin + H2O
Arg-Pro-Pro-Gly + Phe-Ser-Pro-Phe-Arg
show the reaction diagram
casein + H2O
?
show the reaction diagram
-
-
-
-
-
catalytic subunit of protein kinase A + H2O
?
show the reaction diagram
-
in rat kidney
-
-
?
CCK8 nonsulfated + H2O
?
show the reaction diagram
CCK8NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
cerulein + H2O
?
show the reaction diagram
cholecystokinin 8-sulfate + H2O
?
show the reaction diagram
-
-
-
?
collagen I + H2O
?
show the reaction diagram
-
-
-
?
Collagen IV + H2O
?
show the reaction diagram
collagen type IV + H2O
?
show the reaction diagram
Collagen type IV + H2O
Hydrolyzed collagen type IV
show the reaction diagram
-
-
-
-
collagen type V + H2O
?
show the reaction diagram
-
substrate identified by 2D IEF/SDS-PAGE-based image analysis
-
-
?
E-cadherin + H2O
?
show the reaction diagram
Endothelin I + H2O
?
show the reaction diagram
-
rat
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fibrillar procollagen type I + H2O
fibrillar collagen type I + fibrillar collagen type I propeptide
show the reaction diagram
fibrillar procollagen type III + H2O
fibrillar collagen type III + fibrillar collagen type I propeptide
show the reaction diagram
Fibronectin + H2O
?
show the reaction diagram
Fibronectin + H2O
Hydrolyzed fibronectin
show the reaction diagram
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-
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gastrin + H2O
?
show the reaction diagram
-
-
-
-
?
gastrin 17 + H2O
?
show the reaction diagram
-
mutant Y199K
-
?
gastrin-releasing peptide + H2O
?
show the reaction diagram
gastrin-releasing peptide-(14-27) + H2O
?
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
Gelatin + H2O
Hydrolyzed gelatin
show the reaction diagram
-
-
-
-
ghrelin + H2O
?
show the reaction diagram
Glucagon + H2O
?
show the reaction diagram
Glutaryl-Phe 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
poor substrate, hydrolysis at 2.4% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
-
Gonadotropin + H2O
?
show the reaction diagram
-
mouse
-
-
-
GRP-(14-27) + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
Hemoglobin + H2O
?
show the reaction diagram
-
poor substrate
-
-
-
Human alpha-atrial natiuretic peptide + H2O
?
show the reaction diagram
Human transforming growth factor + H2O
?
show the reaction diagram
-
rat
-
-
-
insulin + H2O
?
show the reaction diagram
-
in mouse kidney
-
-
?
insulin B chain + H2O
?
show the reaction diagram
-
in human small intestine
-
-
?
Insulin B-chain + H2O
Hydrolyzed insulin B-chain
show the reaction diagram
interleukin-1beta + H2O
?
show the reaction diagram
-
meprin beta activates interleukin 18
-
-
?
interleukin-6 + H2O
?
show the reaction diagram
L-Leu 2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
-
Laminin + H2O
?
show the reaction diagram
laminin + H2O
fragments of laminin
show the reaction diagram
-
-
-
-
laminin 1 + H2O
?
show the reaction diagram
-
human homomeric recombinant enzyme
-
-
?
laminin 5 + H2O
?
show the reaction diagram
-
human homomeric recombinant enzyme
-
-
?
Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
poor substrate, hydrolysis at 6.2% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
-
Luliberin + H2O
?
show the reaction diagram
-
i.e. luteinizing-hormone releasing hormone, preferred cleavage site: Trp3-Ser4, other sites are Ser4-Tyr5 and Tyr5-Gly6
-
-
-
luteinizing hormone releasing hormone LHRH + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
luteinizing-hormone-releasing hormone + H2O
?
show the reaction diagram
lysyl oxidase + H2O
?
show the reaction diagram
-
substrate identified by 2D IEF/SDS-PAGE-based image analysis
-
-
?
miniprocollagen alpha1(I) homotrimers + H2O
?
show the reaction diagram
-
-
-
-
?
MMP1 protein + H2O
?
show the reaction diagram
Muc2 protein + H2O
?
show the reaction diagram
N-Benzoyl-L-tyrosyl-4-aminobenzoate + H2O
N-Benzoyl-L-tyrosine + 4-aminobenzoate
show the reaction diagram
neuropeptide Y + H2O
?
show the reaction diagram
neurotensin + H2O
?
show the reaction diagram
Nidogen + H2O
?
show the reaction diagram
-
in rat kidney
-
-
?
nidogen 1 + H2O
?
show the reaction diagram
nidogen-1 + H2O
?
show the reaction diagram
occludin + H2O
?
show the reaction diagram
orcokinin + H2O
?
show the reaction diagram
-
-
-
-
?
oxytocin + H2O
?
show the reaction diagram
-
in human small intestine
-
-
?
Oxytocin + H2O
Hydrolyzed oxytocin
show the reaction diagram
-
poor substrate
-
-
Parathyroid hormone + H2O
?
show the reaction diagram
Parathyroid hormone + H2O
Hydrolyzed parathyroid hormone
show the reaction diagram
-
i.e. hPTH-(1-84), in vivo and in vitro
-
-
parathyroid hormone fragment 13-34 + H2O
?
show the reaction diagram
pro-interleukin 1beta + H2O
interleukin 1beta + H2O
show the reaction diagram
cleavage at the H115-D116 bond, which is one amino acid N-terminal to the caspase-1 cleavage site and five amino acids C-terminal to the meprin beta site. Both oligomeric meprin A and recombinant meprin alpha are capable of cleaving
the biological activity of the pro-interleukin-1beta cleaved product produced by meprin A, is 3fold higher to that of the interleukin-1beta product produced by meprin b or caspase-1
-
?
Pro-interleukin-1beta + H2O
?
show the reaction diagram
-
-
-
-
?
pro-KLK7 + H2O
KLK7 + ?
show the reaction diagram
-
meprin beta cleaves pro-KLK7 between Gly17 and Asp18
-
-
?
procollagen I + H2O
collagen I + propeptide of collagen III
show the reaction diagram
procollagen III + H2O
?
show the reaction diagram
-
meprin alpha can process procollagen III
-
-
?
procollagen type III + H2O
?
show the reaction diagram
-
human homomeric recombinant enzyme
-
-
?
protein GRP + H2O
?
show the reaction diagram
protein kinase A + H2O
?
show the reaction diagram
-
the enzyme cleaves at defined sites, isoform-specific interactions between the catalytic subunit of PKA (PKA C) and meprins, overview
-
-
?
protein kinase A catalytic subunit Cbeta1 + H2O
?
show the reaction diagram
-
the enzyme cleaves at defined sites, cytosolic-enriched kidney proteins from meprin alphabeta double knockout mice, and purified forms of recombinant mouse PKA Calpha, Cbeta1, and Cbeta2, are incubated with activated forms of either homomeric meprinA or meprin B, EC 3.4.24.63, product analysis by mass spectrometry, overview. Meprin A only cleaves PKA Cbeta1
-
-
?
protein LHRH + H2O
?
show the reaction diagram
protein PTH12-34 + H2O
?
show the reaction diagram
sCCK8NH2 + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
Secretin + H2O
?
show the reaction diagram
Substance P + H2O
?
show the reaction diagram
Substance P + H2O
Hydrolyzed substance P
show the reaction diagram
Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O
?
show the reaction diagram
-
arylamidolysis
-
-
-
Succinyl-Ala-Ala-Pro-Phe 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
poor substrate, hydrolysis at 9% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
-
Succinyl-Ala-Pro-Ala 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
poor substrate, hydrolysis at 2.8% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
-
Succinyl-Gly-Pro 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
poor substrate, hydrolysis at 10.7% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
-
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Succinyl-Leu-Tyr 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
poor substrate, hydrolysis at 5.6% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
-
tenascin-C + H2O
?
show the reaction diagram
tissue growth factor-alpha + H2O
?
show the reaction diagram
-
meprin alpha can process tissue growth factor-alpha
-
-
?
tumor necrosis factor alpha + H2O
?
show the reaction diagram
-
in rat kidney
-
-
?
Tyr 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
poor substrate, hydrolysis at 3.3% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
-
Tyr-Leu-Val-Cys(SO3-)-Gly-Glu-Arg-Gly + H2O
?
show the reaction diagram
valosin + H2O
?
show the reaction diagram
vascular endothelial growth factor A + H2O
?
show the reaction diagram
-
-
-
-
?
vascular endothelial growth factor-A + H2O
?
show the reaction diagram
Vasoactive intestinal peptide + H2O
?
show the reaction diagram
VEGF-A + H2O
?
show the reaction diagram
-
human meprin alpha cleaves VEGF-A in vitro, generating proteolytic fragments as found in wild-type zebrafish
-
-
?
villin + H2O
?
show the reaction diagram
-
murine homomeric recombinant enzyme
-
-
?
vinculin + H2O
?
show the reaction diagram
-
substrate identified by 2D IEF/SDS-PAGE-based image analysis
-
-
?
[Met5]enkephalin-Arg6-Phe7 + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-melanocyte-stimulating hormone + H2O
?
show the reaction diagram
-
-
-
-
?
Angiotensin I + H2O
?
show the reaction diagram
Angiotensin II + H2O
?
show the reaction diagram
B-type natriuretic peptide + H2O
?
show the reaction diagram
-
-
-
-
?
bombesin + H2O
?
show the reaction diagram
-
-
-
-
?
Bradykinin + H2O
?
show the reaction diagram
catalytic subunit of protein kinase A + H2O
?
show the reaction diagram
-
in rat kidney
-
-
?
CCK8NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
cerulein + H2O
?
show the reaction diagram
-
-
-
-
?
Collagen IV + H2O
?
show the reaction diagram
-
in rat kidney
-
-
?
collagen type IV + H2O
?
show the reaction diagram
-
meprin alpha can degrade collagen type IV
-
-
?
E-cadherin + H2O
?
show the reaction diagram
fibrillar procollagen type I + H2O
fibrillar collagen type I + fibrillar collagen type I propeptide
show the reaction diagram
fibrillar procollagen type III + H2O
fibrillar collagen type III + fibrillar collagen type I propeptide
show the reaction diagram
Fibronectin + H2O
?
show the reaction diagram
gastrin-releasing peptide-(14-27) + H2O
?
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
in rat kidney
-
-
?
Glucagon + H2O
?
show the reaction diagram
-
-
-
-
?
insulin + H2O
?
show the reaction diagram
-
in mouse kidney
-
-
?
insulin B chain + H2O
?
show the reaction diagram
-
in human small intestine
-
-
?
interleukin-1beta + H2O
?
show the reaction diagram
-
meprin beta activates interleukin 18
-
-
?
Laminin + H2O
?
show the reaction diagram
-
in rat kidney
-
-
?
luteinizing-hormone-releasing hormone + H2O
?
show the reaction diagram
MMP1 protein + H2O
?
show the reaction diagram
Muc2 protein + H2O
?
show the reaction diagram
neuropeptide Y + H2O
?
show the reaction diagram
-
-
-
-
?
neurotensin + H2O
?
show the reaction diagram
Nidogen + H2O
?
show the reaction diagram
-
in rat kidney
-
-
?
nidogen 1 + H2O
?
show the reaction diagram
occludin + H2O
?
show the reaction diagram
oxytocin + H2O
?
show the reaction diagram
-
in human small intestine
-
-
?
Parathyroid hormone + H2O
?
show the reaction diagram
parathyroid hormone fragment 13-34 + H2O
?
show the reaction diagram
-
-
-
-
?
Pro-interleukin-1beta + H2O
?
show the reaction diagram
-
-
-
-
?
procollagen I + H2O
collagen I + propeptide of collagen III
show the reaction diagram
-
meprin alpha removes both the C- and N-propeptides of type I procollagen, subsequently releasing fibril-forming mature collagen molecules. The C-terminal cleavage sites in the proalpha1(I) chain generated by the enzyme is identified as Ala1218/Asp1219, identical to the BMP-1 cleavage site, and also Arg1227/Asp1228, nine residues C-terminal to the BMP-1 cleavage site
-
-
?
procollagen III + H2O
?
show the reaction diagram
-
meprin alpha can process procollagen III
-
-
?
protein kinase A + H2O
?
show the reaction diagram
-
the enzyme cleaves at defined sites, isoform-specific interactions between the catalytic subunit of PKA (PKA C) and meprins, overview
-
-
?
Secretin + H2O
?
show the reaction diagram
-
-
-
-
?
Substance P + H2O
?
show the reaction diagram
tenascin-C + H2O
?
show the reaction diagram
tissue growth factor-alpha + H2O
?
show the reaction diagram
-
meprin alpha can process tissue growth factor-alpha
-
-
?
tumor necrosis factor alpha + H2O
?
show the reaction diagram
-
in rat kidney
-
-
?
valosin + H2O
?
show the reaction diagram
-
-
-
-
?
vascular endothelial growth factor A + H2O
?
show the reaction diagram
-
-
-
-
?
Vasoactive intestinal peptide + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Calcium
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2-(N-Hydroxycarboxamido)-4-methylpentanoyl-L-alanyl-glycylamide
-
i.e. Zinkov inhibitor
2-mercaptoethanol
3-[(E)-[4-formyl-5-hydroxy-6-methyl-3-[(phosphonooxy)methyl]pyridin-2-yl]diazenyl]-7-nitronaphthalene-1,5-disulfonic acid
-
competitive inhibition
Acetyl-Arg-Pro-Gly-Tyr hydroxamate
-
kinetics
actinonin
Arg-Pro-Pro-Gly-(4-nitro)Phe-Glu-Pro-Phe-Arg
-
-
batimastat
-
-
Ca2+
-
above 1 mM, metalloproteinase, does not require additional Ca2+
captopril
CGS 27023A
chymostatin
CONA 65
CONA 68
CONA 71
CONA 73
cystatin C
-
-
-
cysteine
dithioerythritol
-
-
DL-Pro-DL-Leu-Gly-NH2
-
-
doxycycline
fetuin-A
-
-
-
galardin
-
-
GM6001
homophenylalanine hydroxamate
Hydroxamyl-succinyl-Pro-Phe-Arg
-
kinetics
L-Pro-L-Leu-Gly-hydroxamate
-
-
N-isobutyl-N-(4-methoxyphenylsulfonyl)glycyl hydroxamic acid
-
-
N-[1-[(1-amino-1-oxopropan-2-yl)amino]-3-(naphthalen-1-yl)-1-oxopropan-2-yl]-4-(hydroxyamino)-2-(2-methylpropyl)pent-4-enamide
-
-
N-[4-(hydroxyamino)-2-(2-methylpropyl)pent-4-enoyl]-3-methylvalyl-N-(2-aminoethyl)-DL-alaninamide
-
-
N4-hydroxy-N1-[3-(1H-indol-3-yl)-1-(methylamino)-1-oxopropan-2-yl]-2-(2-methylpropyl)butanediamide
-
-
Pro-Leu-Gly hydroxamate
Pro-Leu-Gly-hydroxamate
Ro 32-7315
-
-
TAPI-0
-
i.e. tumour necrosis factor alpha protease inhibitor-0
TAPI-1
a hydroxamate inhibitor; a hydroxamate inhibitor
TAPI-2
-
i.e. tumour necrosis factor alpha protease inhibitor-2
TNF-a processing enzyme inhibitor
-
TAPI-2
-
TNF-alpha processing enzyme inhibitor
-
TAPI-2
-
tyrosine hydroxamate
verapamil
-
verapamil at 1 mg/kg, i.v. can efficiently prevent the ischemic acute kidney injury in female rats, as well as male rats
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KLK4
-
activator of meprin beta
-
KLK5
-
activator of meprin alpha and beta
-
KLK8
-
activator of meprin beta
-
neutrophil elastase
-
activator of meprin alpha
-
plasmin
-
activator of meprin alpha
-
Trypsin
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.22
2-Aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
-
0.183
2-Aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
-
1.38
2-Aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg
-
-
1.22
2-Aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg
-
-
2.46
2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg
-
-
0.402
2-Aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg
-
-
0.296
2-Aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
-
0.0223 - 0.292
alpha-MSH
0.331
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg
-
-
0.174
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg
-
-
0.182
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg
-
-
0.226
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg
-
-
0.29
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg
-
-
0.0121 - 0.224
bradykinin
0.11 - 0.49
cholecystokinin 8-sulfate
0.2
gastrin
-
hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min
0.44
gastrin 17
-
pH 7.5, 37C, mutant Y199K
0.11
GRP
-
hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min
-
0.116
GRP-(14-27)
-
pH 7.5, 37C
0.0565 - 0.156
LHRH
0.0732
orcokinin
-
hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min
-
0.0296
protein GRP
-
meprin A hydrolysis in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 between 8-30 min
-
0.018 - 0.0672
protein PTH12-34
0.0339 - 0.111
secretin
0.0306 - 0.118
Substance P
0.12
valosin
-
pH 7.5, 37C
-
additional information
additional information
-
Michaelis-Menten enzyme kinetics, overview
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1
2-Aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg
Mus musculus
-
-
26.7
2-Aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg
Mus musculus
-
-
98.5
2-Aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg
Mus musculus
-
-
4.9
2-Aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg
Mus musculus
-
-
12
2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg
Mus musculus
-
-
2.4
2-Aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg
Mus musculus
-
-
133
2-Aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg
Mus musculus
-
-
16.5 - 45.4
alpha-MSH
51.5
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg
Mus musculus
-
-
19.6
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg
Mus musculus
-
-
5
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg
Mus musculus
-
-
7.6
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg
Mus musculus
-
-
40.9
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg
Mus musculus
-
-
3.7 - 22
bradykinin
3.8 - 14.2
cholecystokinin 8-sulfate
0.4
gastrin
Homo sapiens
-
-
10.8
gastrin 17
Mus musculus
-
pH 7.5, 37C, mutant Y199K
17.4
glucagon
Mus musculus
-
pH 7.5, 37C
6.3
orcokinin
Homo sapiens
-
-
-
4.6
parathyphoid hormone 12-34
Homo sapiens
-
-
19.6 - 24.6
protein GRP
5.1 - 39.4
protein LHRH
10.5
protein PTH12-34
Mus musculus
-
-
4.4 - 11.8
secretin
5.2 - 12.1
Substance P
18.8
valosin
Mus musculus
-
pH 7.5, 37C
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00002
actinonin
0.0044
batimastat
0.074 - 0.74
captopril
0.00053
DL-Pro-DL-Leu-Gly-NH2
-
pH and temperature not specified in the publication
0.00014 - 0.00017
galardin
0.00053
L-Pro-L-Leu-Gly-hydroxamate
-
pH 7.5, 37C
0.0004
N-isobutyl-N-(4-methoxyphenylsulfonyl)glycyl hydroxamic acid
-
pH 7.5, 37C
0.0022
N-[1-[(1-amino-1-oxopropan-2-yl)amino]-3-(naphthalen-1-yl)-1-oxopropan-2-yl]-4-(hydroxyamino)-2-(2-methylpropyl)pent-4-enamide
-
pH and temperature not specified in the publication
0.0015
N-[4-(hydroxyamino)-2-(2-methylpropyl)pent-4-enoyl]-3-methylvalyl-N-(2-aminoethyl)-DL-alaninamide
-
pH and temperature not specified in the publication
0.0004
N4-hydroxy-N1-[3-(1H-indol-3-yl)-1-(methylamino)-1-oxopropan-2-yl]-2-(2-methylpropyl)butanediamide
-
pH and temperature not specified in the publication
0.0016
Ro 32-7315
-
pH 7.5, 37C
0.0022
TAPI-0
-
pH 7.5, 37C
0.0015
TAPI-2
-
pH 7.5, 37C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.067
3-[(E)-[4-formyl-5-hydroxy-6-methyl-3-[(phosphonooxy)methyl]pyridin-2-yl]diazenyl]-7-nitronaphthalene-1,5-disulfonic acid
Homo sapiens
-
pH 7.5, 25C
0.0001 - 0.18
actinonin
40
captopril
Homo sapiens
-
-
0.008 - 0.016
CGS 27023A
0.0033 - 2
CONA 65
0.04 - 0.05
CONA 68
0.17 - 0.25
CONA 71
0.065 - 0.31
CONA 73
12 - 20
doxycycline
0.06 - 0.6
GM6001
0.1 - 0.8
homophenylalanine hydroxamate
0.4 - 16
Pro-Leu-Gly hydroxamate
0.08 - 0.8
TNF-alpha processing enzyme inhibitor
0.1 - 1.1
tyrosine hydroxamate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00363
-
succinyl-Ala-Ala-Ala 4-nitroanilide
0.095
-
Tyr-Leu-Val-Cys(SO3-)-Gly-Glu-Arg-Gly
0.9
-
mutant N152Q
1.38
-
oxidized insulin B-chain, trypsin-treated enzyme
1.4
-
mutant N426Q and N41Q
1.6
-
mutant N614Q
2
-
mutant N270Q
2.1
-
mutant N330Q
2.2
-
mutant N546Q
2.4
-
mutant N234Q
3.2
-
wild-type
3.3
-
mutant N553Q
4.2
-
specific activity of homo-oligomeric meprin A against PTH
4.54
-
n-bradykinin
5.9
-
mutant N452Q
20.8
-
wild-type
2700
-
specific activity of homo-oligomeric meprin A against gelatin
20500
-
specific activity of homo-oligomeric meprin A against azocasein
20800
-
specific activity of homo-oligomeric meprin A against azocasein
25000
-
specific activity of homo-oligomeric meprin A against gelatin
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
gelatin as substrate
7.4
-
assay at
7.5 - 8
-
-
9.5
-
azocasein as substrate
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
-
rapid decrease of activity below 5 and above 9
7 - 8
-
plateau with maximal activity at pH 7.5-8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
Madin-Darby canine kidney epithelial cells
Manually annotated by BRENDA team
-
in human epidermis, meprin alpha is expressed exclusively in the keratinocytes of the stratum basale
Manually annotated by BRENDA team
-
in human epidermis, meprin beta is restricted to the cells of the stratum granulosum
Manually annotated by BRENDA team
additional information