Information on EC 3.4.23.B3 - equine infectious anemia virus proteinase

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The expected taxonomic range for this enzyme is: Equine infectious anemia virus

EC NUMBER
COMMENTARY hide
3.4.23.B3
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
equine infectious anemia virus proteinase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Processing at the authentic HIV-1 PR recognition site and release of the mature p17 matrix and the p24 capsid protein, as a result of the cleavage of the -SQNY-/-PIVQ- cleavage site.
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
144114-21-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
AAQTCYNCGK + H2O
AAQTC + YNCGK
show the reaction diagram
-
-
-
?
AKLVLAQLSK + H2O
AKLVL + AQLSK
show the reaction diagram
-
-
-
?
APKVCFKCKQ + H2O
APKVC + FKCKQ
show the reaction diagram
-
-
-
?
EIAV Gag-Pol polypeptide + H2O
?
show the reaction diagram
-
several cleavage sites
-
-
?
EIAV Gag-Pol polypetide + H2O
?
show the reaction diagram
-
several cleavage sites
-
-
?
EIAV nucleocapsid protein + H2O
?
show the reaction diagram
Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu + H2O
Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu + p-nitrophenylalanine-Ala-Lys-Ala-Leu
show the reaction diagram
-
-
-
?
GQFVGVTYNL + H2O
GQFVG + VTYNL
show the reaction diagram
-
-
-
?
HIV-1 Gag-Pol polypeptide + H2O
?
show the reaction diagram
-
low activity
-
-
?
KEEIMLAYQG + H2O
KEEIM + LAYQG
show the reaction diagram
-
-
-
?
KMVKCFNCGK + H2O
KMVKCF + NCGK
show the reaction diagram
-
-
-
?
KPIKCWNCGK + H2O
KPIKCW + NCGK
show the reaction diagram
-
-
-
?
LAKALQTGLA + H2O
LAKAL + QTGLA
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 + H2O
Lys-Ala-Arg-Ile-2-aminohexanoic acid + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 + H2O
Lys-Ala-Arg-Leu-2-aminohexanoic acid + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 + H2O
Lys-Ala-Arg-Val-2-aminohexanoic acid + p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Gln-Ala-2-aminohexanoic acid-NH2 + H2O
Lys-Ala-Arg-Val-2-aminohexanoic acid + p-nitrophenylalanine-Gln-Ala-2-aminohexanoic acid-NH2
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 + H2O
Lys-Ala-Arg-Val-2-aminohexanoic acid + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 + H2O
Lys-Ala-Arg-Val-2-aminohexanoic acid + p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Val-Asn-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O
Lys-Ala-Arg-Val-Asn + p-nitrophenylalanine-Glu-Ala-Gly-NH2
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Val-L-2-naphthylalanine-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O
Lys-Ala-Arg-Val-L-2-naphthylalanine + p-nitrophenylalanine-Glu-Ala-Gly-NH2
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 + H2O
Lys-Ala-Arg-Val-Leu + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 + H2O
Lys-Ala-Arg-Val-Phe + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O
Lys-Ala-Arg-Val-Phe + p-nitrophenylalanine-Glu-Ala-Gly-NH2
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O
Lys-Ala-Arg-Val-Trp + p-nitrophenylalanine-Glu-Ala-Gly-NH2
show the reaction diagram
-
-
-
?
Lys-Ala-Arg-Val-Tyr-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O
Lys-Ala-Arg-Val-Tyr + p-nitrophenylalanine-Glu-Ala-Gly-NH2
show the reaction diagram
-
-
-
?
Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn + H2O
Pro-Ser-Glu-Glu-Tyr + Pro-Ile-2-aminohexanoic acid-Ile-Asn
show the reaction diagram
-
-
-
-
?
PSEEYPIMID + H2O
PSEEY + PIMID
show the reaction diagram
-
-
-
?
QKMMLLAKAL + H2O
QKMML + LAKAL
show the reaction diagram
-
-
-
?
QKQTFPIQQK + H2O
QKQTF + PIQQK
show the reaction diagram
-
-
-
?
SQNYPIVQ + H2O
SQNY + PIVQ
show the reaction diagram
-
-
-
?
STGVFWVENI + H2O
STGVF + WVENI
show the reaction diagram
-
-
-
?
Val-Ser-Gln-Ala-Tyr-Pro-Ile-Val-Gln + H2O
Val-Ser-Gln-Ala-Tyr + Pro-Ile-Val-Gln
show the reaction diagram
good substrate
-
-
?
Val-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln + H2O
Val-Ser-Gln-Asn-Tyr + Pro-Ile-Val-Gln
show the reaction diagram
Val-Ser-Gln-Cys-Tyr-Pro-Ile-Val-Gln + H2O
Val-Ser-Gln-Cys-Tyr + Pro-Ile-Val-Gln
show the reaction diagram
best peptide substrate
-
-
?
Val-Ser-Gln-Gly-Tyr-Pro-Ile-Val-Gln + H2O
Val-Ser-Gln-Gly-Tyr + Pro-Ile-Val-Gln
show the reaction diagram
low activity
-
-
?
Val-Ser-Gln-Ile-Tyr-Pro-Ile-Val-Gln + H2O
Val-Ser-Gln-Ile-Tyr + Pro-Ile-Val-Gln
show the reaction diagram
-
-
-
?
Val-Ser-Gln-Leu-Tyr-Pro-Ile-Val-Gln + H2O
Val-Ser-Gln-Leu-Tyr + Pro-Ile-Val-Gln
show the reaction diagram
-
-
-
?
Val-Ser-Gln-Phe-Tyr-Pro-Ile-Val-Gln + H2O
Val-Ser-Gln-Phe-Tyr + Pro-Ile-Val-Gln
show the reaction diagram
low activity
-
-
?
Val-Ser-Gln-Thr-Tyr-Pro-Ile-Val-Gln + H2O
Val-Ser-Gln-Thr-Tyr + Pro-Ile-Val-Gln
show the reaction diagram
-
-
-
?
Val-Ser-Gln-Val-Tyr-Pro-Ile-Val-Gln + H2O
Val-Ser-Gln-Val-Tyr + Pro-Ile-Val-Gln
show the reaction diagram
-
-
-
?
VFQNYPIVQ + H2O
VFQNY + PIVQ
show the reaction diagram
-
at P4 position the hydrophobic residues Val, Leu, Phe and Pro are preferred
-
-
?
VLQNYPIVQ + H2O
VLQNY + PIVQ
show the reaction diagram
-
at P4 position the hydrophobic residues Val, Leu, Phe and Pro are preferred
-
-
?
VPQNYPIVQ + H2O
VPQNY + PIVQ
show the reaction diagram
-
at P4 position the hydrophobic residues Val, Leu, Phe and Pro are preferred
-
-
?
VSANYPIVQ + H2O
VSANY + PIVQ
show the reaction diagram
-
Gln is preferred at position P3. The second-best residue at P3 position is Phe. Gly and Ala are also tolerated
-
-
?
VSFNYPIVQ + H2O
VSFNY + PIVQ
show the reaction diagram
-
Gln is preferred at position P3. The second-best residue at P3 position is Phe. Gly and Ala are also tolerated
-
-
?
VSGNYPIVQ + H2O
VSGNY + PIVQ
show the reaction diagram
-
Gln is preferred at position P3. The second-best residue at P3 position is Phe. Gly and Ala are also tolerated
-
-
?
VSQNAPIVQ + H2O
VSQNA + PIVQ
show the reaction diagram
-
Phe, Tyr, Leu or Met, Ala are preferred at position P1 in order of decreasing efficiency
-
-
?
VSQNFPIVQ + H2O
VSQNF + PIVQ
show the reaction diagram
-
Phe, Tyr, Leu or Met, Ala are preferred at position P1 in order of decreasing efficiency
-
-
?
VSQNLPIVQ + H2O
VSQNL + PIVQ
show the reaction diagram
-
Phe, Tyr, Leu or Met, Ala are preferred at position P1 in order of decreasing efficiency
-
-
?
VSQNMPIVQ + H2O
VSQNM + PIVQ
show the reaction diagram
-
Phe, Tyr, Leu or Met, Ala are preferred at position P1 in order of decreasing efficiency
-
-
?
VSQNYPIV + H2O
VSQNY + PIV
show the reaction diagram
-
-
-
?
VSQNYPIVQ + H2O
VSQNY + PIVQ
show the reaction diagram
VVQNYPIVQ + H2O
VVQNY + PIVQ
show the reaction diagram
-
at P4 position the hydrophobic residues Val, Leu, Phe and Pro are preferred
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
EIAV Gag-Pol polypetide + H2O
?
show the reaction diagram
-
several cleavage sites
-
-
?
EIAV nucleocapsid protein + H2O
?
show the reaction diagram
-
processing in the early phase of virus replication
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
enzyme assay in presence of 2 M NaCl
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
an oligopeptide substrate representing the cleavage site between the matrix and capsid proteins of HIV-1 is highly active, while its P1'-pipecolic acid-substituted derivative is a specific inhibitor
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.35
AAQTCYNCGK
-
pH 5.6, 37C
0.02
AKLVLAQLSK
-
pH 5.6, 37C
0.6
APKVCFKCKQ
-
pH 5.6, 37C
0.01
Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu
-
pH 4.7, wild-type enzyme
0.47
KMVKCFNCGK
-
pH 5.6, 37C
0.02
KPIKCWNCGK
-
pH 5.6, 37C
0.02 - 0.075
Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2
0.01 - 0.105
Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2
0.01 - 0.03
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2
0.1
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Gln-Ala-2-aminohexanoic acid-NH2
-
pH 4.7, 25C, wild-type enzyme
0.02 - 0.07
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2
0.04 - 0.21
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2
0.007
Lys-Ala-Arg-Val-Asn-p-nitrophenylalanine-Glu-Ala-Gly-NH2
-
pH 4.7, 25C, wild-type enzyme
0.025 - 0.05
Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2
0.035 - 0.045
Lys-Ala-Arg-Val-Nal-p-nitrophenylalanine-Glu-Ala-Gly-NH2
0.035 - 0.07
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2
0.075 - 0.205
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2
0.05 - 0.55
Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2
0.06 - 0.165
Lys-Ala-Arg-Val-Tyr-p-nitrophenylalanine-Glu-Ala-Gly-NH2
0.1
Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn
-
pH 4.7, wild-type enzyme and mutant enzyme T30D
0.08
PSEEYPIMID
-
pH 5.6, 37C
0.27
QKQTFPIQQK
-
pH 5.6, 37C
-
2.2
SQNYPIVQ
-
pH 5.6, 37C
0.04
VSQNYPIV
-
pH 5.6, 37C
0.31
VSQNYPIVQ
-
pH 5.6, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
AAQTCYNCGK
equine infectious anemia virus
-
pH 5.6, 37C
0.4
AKLVLAQLSK
equine infectious anemia virus
-
pH 5.6, 37C
0.2
APKVCFKCKQ
equine infectious anemia virus
-
pH 5.6, 37C
1.9 - 3
Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu
2.5
KEEIMLAYQG
equine infectious anemia virus
-
pH 5.6, 37C
1.2
KMVKCFNCGK
equine infectious anemia virus
-
pH 5.6, 37C
0.02
KPIKCWNCGK
equine infectious anemia virus
-
pH 5.6, 37C
1.4 - 7.2
Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2
1 - 6.3
Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2
0.2 - 1.5
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2
2.6
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Gln-Ala-2-aminohexanoic acid-NH2
equine infectious anemia virus
-
pH 4.7, 25C, wild-type enzyme
0.8 - 8.2
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2
1.3 - 4.2
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2
0.1
Lys-Ala-Arg-Val-Asn-p-nitrophenylalanine-Glu-Ala-Gly-NH2
equine infectious anemia virus
-
pH 4.7, 25C, wild-type enzyme
1.3 - 12.5
Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2
1.5 - 1.6
Lys-Ala-Arg-Val-Nal-p-nitrophenylalanine-Glu-Ala-Gly-NH2
2.2 - 12.5
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2
1 - 4.2
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2
0.5 - 0.8
Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2
0.3
Lys-Ala-Arg-Val-Tyr-p-nitrophenylalanine-Glu-Ala-Gly-NH2
equine infectious anemia virus
-
pH 4.7, 25C, mutant enzyme I54G
0.6 - 1.7
Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn
6.1
PSEEYPIMID
equine infectious anemia virus
-
pH 5.6, 37C
1.3
QKMMLLAKAL
equine infectious anemia virus
-
pH 5.6, 37C
-
10.4
QKQTFPIQQK
equine infectious anemia virus
-
pH 5.6, 37C
-
1.7
SQNYPIVQ
equine infectious anemia virus
-
pH 5.6, 37C
3.7
VSQNYPIVQ
equine infectious anemia virus
-
pH 5.6, 37C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 6
-
activity range with peptide substrates in presence of NaCl
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11431
-
2 * 10000-12000, SDS-PAGE, 2 * 11431, sequence calculation and mass spectrometry
12000
-
x * 12000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 12000, SDS-PAGE
additional information
structure molecular modeling, S2 subsite sequence comparison
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
-
the retropepsin is expressed as part of the Gag-Pol polyprotein precursor, the enzyme cleaves the precursor into functional proteins during or after assembly and budding of the virions, thereby releasing itself
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
complex of the 154G mutant enzyme with the inhibitor HBY-793, hanging-drop vapor diffusion method
-
crystal structure analysis, structure-sequence comparison, molecular modeling, comparison to other retroviruses, overview
crystal structure determination and analysis
-
crystal structure of the enzyme in complex with LP-130, hanging-drop vapor diffusion method, crystals grown at room temperature
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme from virus grown in chronically infected dog thymus cells, ATCC CRL 1430, recombinant enzyme from Escherichia coli by several different steps of ion exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
expression in Escherichia coli as a Pol precursor protein
-
expression of the mutant enzyme I54G in Escherichia coli. The wild-type enzyme does not accumulate in Escherichia coli at a level sufficient for purifying enough material to homogeneity for crystallization
-
phylogenetic analysis, comparison to other retroviruses, overview
the enzyme is encoded in the 5' part of the pol gene, expression as part of the Gag-Pol polyprotein precursor, expression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
I54G/Y48H
-
specificity constant is only 3fold lower than that measured for the single mutant I54G enzyme, towards the long, pseudosymmetrical peptide substrate Ac-Tyr-Arg-Ala-Arg-Val-Phe-4-nitrophenylalanyl-Val-Arg-Ala-Ala-Lys. The potency of the shorter HBY-793 inhibitor is reduced by 60fold compared to the Ki values measured against the wild-type or the I54G mutant enzyme
T30D
-
mutant enzyme is only marginally different from wild-type enzyme in its susceptibility to most of the inhibitors of the HIV-1/HIV-2 proteinases, except that HBY-793 has a potency that is reduced by ten-fold