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Information on EC 3.4.23.52 - preflagellin peptidase and Organism(s) Methanococcus maripaludis and UniProt Accession A9A677

for references in articles please use BRENDA:EC3.4.23.52
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.52 preflagellin peptidase
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This record set is specific for:
Methanococcus maripaludis
UNIPROT: A9A677 not found.
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Word Map
The taxonomic range for the selected organisms is: Methanococcus maripaludis
The expected taxonomic range for this enzyme is: Archaea, Bacteria
Reaction Schemes
Cleaves the signal peptide of 3 to 12 amino acids from the N-terminal of preflagellin, usually at Arg-Gly-/- or Lys-Gly-/-, to release flagellin.
Synonyms
preflagellin peptidase, peptidase-like enzyme, spaseiii, signal peptidase iii, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
peptidase-like enzyme
-
-
preflagellin peptidase
-
-
Prepilin peptidase
-
-
prepilin-like peptidase
-
-
signal peptidase III
-
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
preflagellin FlaB2 + H2O
flagellin FlaB2 + ?
show the reaction diagram
-
-
-
?
DUF361-containing proteins + H2O
?
show the reaction diagram
EppA specifically cleaves proteins with DUF361-like domains
-
-
?
FlaB2 protein + H2O
?
show the reaction diagram
-
FlaB2 protein of Methanococcus voltae
-
-
?
MEFMSNKKGASGIGTLIVFIAMVLVAAV + H2O
MEFMSNKKG + ASGIGTLIVFIAMVLVAAV
show the reaction diagram
-
-
-
-
?
MFMSNKKGASGIGTLIVFIAMVLVAAV + H2O
MFMSNKKG + ASGIGTLIVFIAMVLVAAV
show the reaction diagram
-
-
-
-
?
MKEFMSNKKGASGIGTLIVFIAMVLVAAV + H2O
MKEFMSNKKG + ASGIGTLIVFIAMVLVAAV
show the reaction diagram
-
-
-
-
?
MMSNKKGASGIGTLIVFIAMVLVAAV + H2O
MMSNKKG + ASGIGTLIVFIAMVLVAAV
show the reaction diagram
-
-
-
-
?
MNKKGASGIGTLIVFIAMVLVAAV + H2O
MNKKG + ASGIGTLIVFIAMVLVAAV
show the reaction diagram
-
-
-
-
?
MSNKKGASGIGTLIVFIAMVLVAAV + H2O
MSNKKG + ASGIGTLIVFIAMVLVAAV
show the reaction diagram
-
-
-
-
?
prearchaellin + H2O
archaellin + ?
show the reaction diagram
-
-
-
-
?
preflagellin FlaB1 + H2O
flagellin FlaB1 + ?
show the reaction diagram
-
-
-
-
?
preflagellin FlaB2 + H2O
flagellin + ?
show the reaction diagram
-
-
-
?
preflagellin FlaB2 + H2O
flagellin FlaB2 + ?
show the reaction diagram
preflagellin FlaB2 + H2O
MKIKEFMSNKKG + flagellin
show the reaction diagram
-
preflagellin is MKIKEFMSNKKGASGIGTLIVFIAMVLVAAV, the enzyme cleaves the 12 amino acid N-terminal signal peptide from preflagellinFlaB2 between Gly and L-Ala
-
-
?
prepilin EpdA + H2O
pilin EpdA + EpdA signal peptide
show the reaction diagram
-
cleavage sequence of pilins EpdA signal peptide is MFKRFNRG-QISFE
-
-
?
prepilin EpdB + H2O
pilin EpdB + EpdB signal peptide
show the reaction diagram
-
cleavage sequence of pilins EpdB signal peptide is MSKG-QVSVE
-
-
?
prepilin EpdC + H2O
pilin EpdC + EpdC signal peptide
show the reaction diagram
-
cleavage sequence of pilins EpdC signal peptide is MIKMLQLPFNKKG-QVSFD
-
-
?
prepilin EpdD + H2O
pilin EpdD + EpdD signal peptide
show the reaction diagram
-
cleavage sequence of pilins EpdD signal peptide is MSVALKKFFSKRG-QLSLE
-
-
?
prepilin EpdE + H2O
pilin EpdE + EpdE signal peptide
show the reaction diagram
-
cleavage sequence of pilins EpdE signal peptide is MKFLEKLTSKKG-QIAME
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
prearchaellin + H2O
archaellin + ?
show the reaction diagram
-
-
-
-
?
prepilin EpdA + H2O
pilin EpdA + EpdA signal peptide
show the reaction diagram
-
cleavage sequence of pilins EpdA signal peptide is MFKRFNRG-QISFE
-
-
?
prepilin EpdB + H2O
pilin EpdB + EpdB signal peptide
show the reaction diagram
-
cleavage sequence of pilins EpdB signal peptide is MSKG-QVSVE
-
-
?
prepilin EpdC + H2O
pilin EpdC + EpdC signal peptide
show the reaction diagram
-
cleavage sequence of pilins EpdC signal peptide is MIKMLQLPFNKKG-QVSFD
-
-
?
prepilin EpdD + H2O
pilin EpdD + EpdD signal peptide
show the reaction diagram
-
cleavage sequence of pilins EpdD signal peptide is MSVALKKFFSKRG-QLSLE
-
-
?
prepilin EpdE + H2O
pilin EpdE + EpdE signal peptide
show the reaction diagram
-
cleavage sequence of pilins EpdE signal peptide is MKFLEKLTSKKG-QIAME
-
-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KCl
-
preflagellin peptidase activity is optimal in the presence of 0.4 M KCl with 0.25% (v/v) Triton X-100
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
additional information
-
in Methanococcus maripaludis, the archaella are composed of three structural glycoproteins (the archaellins, FlaB1, FlaB2 and FlaB3) that are all modified at multiple positions with an N-linked tetrasaccharide
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 15000-17000, recombinant EpdE from purified pili, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
EpdE is a glycoprotein with multiple sites occupied by an N-linked glycan
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 30% (w/v) PEG 300, 50 mM glycine (pH 9.5) and 100 mM NaCl
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E25C/I206C
the mutant is proteolytically active
additional information
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Triton X-100
-
preflagellin peptidase activity is optimal in the presence of 0.4 M KCl with 0.25% (v/v) Triton X-100
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
metal-affinity column chromatography and gel filtration
crude membrane fraction
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta 2 (DE3) cells
expressed in an Methanococcus maripaludis DELTAflaK strain
-
expressed in Escherichia coli K113 cell membranes
gene eppA, recombinant expression of C-terminally FLAG- or His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)
-
gene flak, recombinant expression of C-terminally FLAG- or His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Szabo, Z.; Stahl, A.O.; Albers, S.V.; Kissinger, J.C.; Driessen, A.J.; Pohlschroeder, M.
Identification of diverse archaeal proteins with class III signal peptides cleaved by distinct archaeal prepilin peptidases
J. Bacteriol.
189
772-778
2007
Methanococcus maripaludis (Q6LZR9), Methanococcus maripaludis
Manually annotated by BRENDA team
Ng, S.Y.; VanDyke, D.J.; Chaban, B.; Wu, J.; Nosaka, Y.; Aizawa, S.; Jarrell, K.F.
Different minimal signal peptide lengths recognized by the archaeal prepilin-like peptidases FlaK and PibD
J. Bacteriol.
191
6732-6740
2009
Methanococcus maripaludis, Methanococcus voltae, Saccharolobus solfataricus
Manually annotated by BRENDA team
Bardy, S.L.; Jarrell, K.F.
FlaK of the archaeon Methanococcus maripaludis possesses preflagellin peptidase activity
FEMS Microbiol. Lett.
208
53-59
2002
Methanococcus maripaludis (Q6LZR9), Methanococcus maripaludis
Manually annotated by BRENDA team
Correia, J.D.; Jarrell, K.F.
Posttranslational processing of Methanococcus voltae preflagellin by preflagellin peptidases of M. voltae and other methanogens
J. Bacteriol.
182
855-858
2000
Methanocaldococcus jannaschii, Methanococcus maripaludis, Methanococcus vannielii, Methanococcus voltae, Methanothermococcus thermolithotrophicus, no activity in Methanococcus igneus, no activity in Methanoculleus marisnigri, no activity in Methanogenium cariaci, Pseudomonas aeruginosa
Manually annotated by BRENDA team
Hu, J.; Xue, Y.; Lee, S.; Ha, Y.
The crystal structure of GXGD membrane protease FlaK
Nature
475
528-531
2011
Methanococcus maripaludis (A9A677), Methanococcus maripaludis
Manually annotated by BRENDA team
Nair, D.; Jarrell, K.
Pilin processing follows a different temporal route than that of archaellins in Methanococcus maripaludis
Life
5
85-101
2015
Methanococcus maripaludis, Methanococcus maripaludis Mm900
Manually annotated by BRENDA team