Information on EC 3.4.23.41 - yapsin 1

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.23.41
-
RECOMMENDED NAME
GeneOntology No.
yapsin 1
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolyses various precursor proteins with Arg or Lys in P1, and commonly Arg or Lys also in P2. The P3 amino acid is usually non-polar, but otherwise additional basic amino acids are favourable in both non-prime and prime positions
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
-
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CAS REGISTRY NUMBER
COMMENTARY hide
205132-58-7
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
albumin
?
show the reaction diagram
-
-
-
-
-
amidorphin
Met-enkephalin-Lys-Lys
show the reaction diagram
-
-
-
?
aminobenzoyl-Arg-Val-Lys-Arg-Gly-Leu-Ala-Tyr(NO2)-Asp-OH + H2O
?
show the reaction diagram
-
-
?
anglerfish pro-somatostatin I
(Lys-) anglerfish somatostatin 14
show the reaction diagram
-
-
-
?
anglerfish pro-somatostatin II
anglerfish somatostatin 28
show the reaction diagram
-
-
-
?
ATCH1-39 + H2O
?
show the reaction diagram
-
good substrate
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
dynorphin A
Leu-enkephalin-Arg
show the reaction diagram
-
-
-
?
dynorphin B
Leu-enkephalin-Arg-Arg
show the reaction diagram
-
-
-
?
human adrenocorticotropic hormone 1-39
human adrenocorticotropic hormone 1-15 + corticotropin-like intermediate lobe peptide 16-39
show the reaction diagram
human parathyroid hormone
?
show the reaction diagram
-
-
-
-
?
human parathyroid hormone + H2O
?
show the reaction diagram
pre-elafin + H2O
elafin + ?
show the reaction diagram
-
cleavage occurs C-terminal to a subset of single Lys residues
-
?
pro-alpha-mating factor
?
show the reaction diagram
-
-
-
-
?
pro-alpha-mating factor + H2O
alpha-mating factor + pro-sequence of alpha-mating factor
show the reaction diagram
-
processing
-
-
?
pro-kexin + H2O
kexin + ?
show the reaction diagram
-
-
-
-
?
pro-somatostatin II + H2O
somatostatin-28 + pro-sequence of somatostatin-28
show the reaction diagram
-
processing, cleavage after a single Arg residue
-
-
?
Proglucagon + H2O
Glucagon + ?
show the reaction diagram
-
-
in vitro processing reaction
-
?
proinsulin
?
show the reaction diagram
-
-
-
-
?
proopiomelanocortin
adrenocorticotropic hormone 1-39 + adrenocorticotropic hormone 1-14
show the reaction diagram
-
-
-
?
synthetic cholecystokinin
?
show the reaction diagram
-
-
-
-
?
synthetic proalbumin
RGVFRR + DEAKSEVAW
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pre-elafin + H2O
elafin + ?
show the reaction diagram
-
cleavage occurs C-terminal to a subset of single Lys residues
-
?
pro-alpha-mating factor + H2O
alpha-mating factor + pro-sequence of alpha-mating factor
show the reaction diagram
-
processing
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
5 mM, 125% of initial activity
Mn2+
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5 mM, 130% of initial activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ba2+
-
5 mM, 85% residual activity
Na+
-
5 mM, 80% residual activity
pepstatin A
RVSMIKNR
-
peptidomimetic inhibitor
additional information
-
Y1, a potent, competitive inhibitor that should serve as a powerful chemical tool for the study of the increasing number of yapsin-like enzymes
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.044
amidorphin
-
-
0.1153
Cholecystokinin
-
-
0.1361
dynorphin A
-
-
0.079
Dynorphin B
-
-
0.0177
human adrenocorticotropic hormone 1-39
-
-
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0.0185
Proinsulin
-
-
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0004
pepstatin A
-
-
0.0001
RVSMIKNR
-
below
additional information
additional information
-
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
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30% of maximum activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
localizes on the cell wall via glycosylphosphatidylinositol anchor; localizes on the cell wall via glycosylphosphatidylinositol anchor
Manually annotated by BRENDA team
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the enzyme is secreted
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
1 * 65000-850000, beta-subunit, + 1 * 25000, alpha-subunit. SDS-PAGE, highly glycosylated protein. x * 55000, calculated for mature protein
31000
-
alpha,beta, 1 * 66000-97000 + 1 * 31000, SDS-PAGE
42000
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x * 42000, SDS-PAGE, x * 42300, calculated
42300
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x * 42000, SDS-PAGE, x * 42300, calculated
55000
1 * 65000-850000, beta-subunit, + 1 * 25000, alpha-subunit. SDS-PAGE, highly glycosylated protein. x * 55000, calculated for mature protein
57000
1 * 150000-200000, SDS-PAGE, highly glycosylated protein. 1 * 57000, calculated for mature protein
60000
-
x * 60000, non-glycosylated enzyme, 70000-150000, glycosylated enzyme
65000 - 100000
PAGE
110000
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gel filtration
150000 - 180000
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gel filtration
150000 - 200000
PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
1 * 65000-850000, beta-subunit, + 1 * 25000, alpha-subunit. SDS-PAGE, highly glycosylated protein. x * 55000, calculated for mature protein
monomer
1 * 150000-200000, SDS-PAGE, highly glycosylated protein. 1 * 57000, calculated for mature protein
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
proteolytic modification
side-chain modification
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glycosylated
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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no loss of activity after 1 h
50
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80% loss of activity after 1 h
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; truncated proyapsin I expressed in baculovirus/Sf9 cells; truncated yapsin I expressed in yeast
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recombinant enzyme lacking the GPI-anchor from yeast, purification using inhibitor RVSMIKNR affinity chromatography
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recombinant truncated enzyme; recombinant truncated enzyme
using Ni-NTA chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
expression in PC12 cells
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expression in Pichia pastoris
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expression of C-terminally truncated proyapsin I in baculovirus/Sf9 cells; expression of C-terminally truncated yapsin I in yeast
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expression of C-terminally truncated yapsin I in yeast
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gene yps1, DNA and amino acid sequence determination and analysis, expression of recombinant enzyme lacking the GPI-anchor in yeast cells
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gene YPS1, expression of wild-type and mutant enzymes in yeast strains
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overexpression in yeast
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
heat-induced expression of YPS1 is regulated predominantly by the calcineurin-Crz1 pathway and partially by the Slt2 MAPK pathway. Loss of transcriptional induction in a calcineurin mutant is only observed for gene YPS1
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YPS1 expression is significantly induced only by a subset of stressor agents, such as sodium dodecyl sulfate and tunicamycin
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D101E/DELTA37
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inactive mutant enzyme, no processing of the 87000 Da Pro-yapsin
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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yapsin 1 displays specific immunoreactivity in the alpha-cells of human pancreatic islets
synthesis