Information on EC 3.4.23.26 - Rhodotorulapepsin

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.23.26
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RECOMMENDED NAME
GeneOntology No.
Rhodotorulapepsin
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Specificity similar to that of pepsin A. Cleaves Z-Lys-/-Ala-Ala-Ala and activates trypsinogen
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
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aspartic endopeptidase
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CAS REGISTRY NUMBER
COMMENTARY hide
37259-59-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
No. 45-2
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser + H2O
?
show the reaction diagram
Benzyloxycarbonyl-Glu-Tyr + H2O
Benzyloxycarbonyl-Glu + Tyr
show the reaction diagram
Benzyloxycarbonyl-Gly-Phe + H2O
Benzyloxycarbonyl-Gly + Phe
show the reaction diagram
Benzyloxycarbonyl-Phe-Tyr + H2O
Benzyloxycarbonyl-Phe + Tyr
show the reaction diagram
casein + H2O
?
show the reaction diagram
Copoly-L-Leu-L-Lys*HBr + H2O
?
show the reaction diagram
Copoly-L-Leu-L-Orn-NH2*HBr + H2O
?
show the reaction diagram
DRVYIHPFHLLVYS + H2O
DRVY + IHPFHLL + VYS
show the reaction diagram
Gly-Leu-Tyr + H2O
Gly-Leu + Tyr
show the reaction diagram
Hemoglobin + H2O
?
show the reaction diagram
Z-Lys-Ala-Ala-Ala + H2O
Z-Lys + Ala-Ala-Ala
show the reaction diagram
Z-Lys-Leu-Ala-Ala + H2O
Z-Lys + Leu-Ala-Ala
show the reaction diagram
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-epoxy-3-(4-nitrophenoxy)propane
-
-
4-bromo-phenacylbromide
-
-
alpha-Diazo-p-bromo acetophenone
diazoacetyl glycine ethyl ester
-
-
Diazoacetyl-DL-norleucine methyl ester
Diazoacetyl-Gly ethyl ester
Hg2+
-
weak
N-Diazoacetyl-N'-2,4-dinitrophenylethylenediamine
-
-
Ni2+
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weak
p-bromophenacyl bromide
-
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PCMB
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weak
pepstatin Ac
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60% inhibition at 0.031 mM, addition to cell culture enhances the cell growth and changes the optimal growth temperature
sodium lauryl sulfate
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Streptomyces-pepsin inhibitor
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sulfhydryl reagents
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Zn2+
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weak
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2 - 2.5
2.5 - 2.7
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casein, hemoglobin
2.5 - 3
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acid denatured hemoglobin
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1 - 3.5
-
1: about 20% of activity maximum, 3.5: about 50% of activity maximum
1 - 4
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1: about 25% of activity maximum, 4.0: about 40% of activity maximum, hemoglobin
1.5 - 6
-
about 25% of activity maximum at pH 1.5 and 6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 65
-
30°C and 65°C: about 40% of activity maximum
37 - 65
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37°C: about 40% of activity maximum, 65°C: about 70% of activity maximum
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
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isoelectric focusing
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000 - 30000
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Rhodotorula glutinis, sedimentation equilibrium method, gel filtration
29000
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gel filtration
30000 - 32000
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Cladosporium sp. No. 45-2, gel filtration, sedimentation equilibrium method
30000
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sedimentation equilibrium analysis
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified native enzyme, 3times crystallization
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 7
-
30°C, 20 h, stable
30715
2.5
-
70°C, 10 min, loss of activity
30715
2.5 - 6.5
-
37°C, 20 h, stable
30717
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
pH 2.5-7.0, 20 h, stable
37
-
pH 2.5-6.5, 20 h, stable
65
-
pH 4.0, 10 min, stable below
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme from strain K-24 by ammonium sulfate fractionation, acetone precipation, gel filtration to homogeneity
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No. 45-2
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