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Information on EC 3.4.23.15 - renin and Organism(s) Canis lupus familiaris and UniProt Accession Q6DYE7

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.15 renin
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This record set is specific for:
Canis lupus familiaris
UNIPROT: Q6DYE7 not found.
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Word Map
The taxonomic range for the selected organisms is: Canis lupus familiaris
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
cleavage of Leu-/- bond in angiotensinogen to generate angiotensin I
Synonyms
renin, prorenin, angiotensinogenase, angiotensin-forming enzyme, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
angiotensin-forming enzyme
-
-
-
-
angiotensinogenase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9015-94-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-(4'-(dimethylaminophenylazo))-benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS + H2O
4-(4'-(dimethylaminophenylazo))-benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu + Val-Ile-His-Thr-EDANS
show the reaction diagram
-
-
-
?
acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser + H2O
acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Leu-Val-Tyr-Ser
show the reaction diagram
-
-
-
?
angiotensinogen + H2O
?
show the reaction diagram
-
role in regulating blood pressure
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
angiotensinogen + H2O
?
show the reaction diagram
-
role in regulating blood pressure
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
transition state analogues
-
regulation of blood pressure
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000006
aliskiren
Canis lupus familiaris
-
0.0008
pepstatin A
Canis lupus familiaris
-
0.0000023
SR 42128
Canis lupus familiaris
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
RENI_CANLF
403
1
44099
Swiss-Prot
Secretory Pathway (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
-
1 * 36000 Da, SDS-PAGE
42000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 36000 Da, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
side-chain modification
-
glycoprotein
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G149S/S150T
mutant enzyme shows lower specific activities than native renin. 50% loss in specific activity with 4-(4'-(dimethylaminophenylazo))-benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS and 4-(4'-(dimethylaminophenylazo))-benzoyl-gamma-aminobutyryl-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS. Mutation has no effect on substrate specificity of canine renin
G149S/S150T/V286L
mutant enzyme shows lower specific activities than native renin
V286L
mutant enzyme shows lower specific activities than native renin
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
full-length prorenin is expressed in HEK 293 cells, purified and converted to its active form by trypsin-mediated cleavage of the 43 residue propeptide
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
full-length prorenin is expressed in HEK 293 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tree, M.; Brown, J.J.; Leckie, B.J.; Lever, A.F.; Manthem, P.; Morton, J.J.; Robertson, J.I.; Szelke, M.; Webb, D.
The renin-angiotensin converting enzyme cascade
Biochem. Soc. Trans.
12
948-951
1984
Canis lupus familiaris, Equus caballus, Homo sapiens, Rattus norvegicus, Sus scrofa
-
Manually annotated by BRENDA team
Inagami, T.
Structure and function of renin
J. Hypertens.
7
S3-S7
1989
Canis lupus familiaris, Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Bukhtiyarov, Y.; Zecher, M.; Panemangalore, R.; Wu, Z.; Bruno, J.G.; Yuan, J.; Xu, Z.; Dillard, L.W.; McGeehan, G.M.; Harrison, R.K.; Scott, B.B.
Cloning, characterization and site-directed mutagenesis of canine renin
J. Biochem.
142
671-680
2007
Canis lupus familiaris (Q6DYE7), Canis lupus familiaris
Manually annotated by BRENDA team