Information on EC 3.4.22.B77 - Sulfolobus solfataricus peptidase

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The expected taxonomic range for this enzyme is: Sulfolobus solfataricus

EC NUMBER
COMMENTARY hide
3.4.22.B77
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
Sulfolobus solfataricus peptidase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of proteins. Preferentially cleaves -Glu-/- bonds. The enzyme is capable of cleaving Tyr-/- and Phe-/- bonds (chymotrypsin-like activity), Lys-/- and Arg-/- bonds (trypsin-like activity). Asp-/- and Asn-/- bonds are cleaved to a much lower extent. The protease degrades relatively large proteins (50 and 200 kDa) to yield small (below 500 Da) peptides.
show the reaction diagram
-
-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Bacillus stearothermophilus alcohol dehydrogenase + H2O
?
show the reaction diagram
L-Glu-7-amido-4-methylcoumarin + H2O
L-Glu + 7-amino-4-methylcoumarin
show the reaction diagram
N-acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O
N-acetyl-Asp-Glu-Val-Asp + 7-amino-4-methylcoumarin + H2O
show the reaction diagram
-
14% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin
-
-
?
N-benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
show the reaction diagram
-
7.1% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin
-
-
?
N-benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
9.5% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin
-
-
?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
N-succinyl-Ala-Glu-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Glu + 7-amino-4-methylcoumarin
show the reaction diagram
-
81% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin
-
-
?
N-succinyl-Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Phe-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DTNB
-
5 mM, 49% inhibition
N-ethylmaleimide
-
5 mM, 95% inhibition
PMSF
-
3 mM, 16% inhibition
additional information
-
no inhibition by 5 mM EDTA, 0.01 mM pepstatin A or 0.1 mM E-64
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.5
-
pH 7.0: about 80% of maximal activity, pH 8.5: about 40% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60 - 80
-
60°C: about 75% of maximal activity, 80°C: about 50% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10.5
-
calculated from sequence
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000
-
gel filtration
11072
-
1 * 11072, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
90 min, prior heating of the protease solution stimulats the hydrolysis rate
80
-
30 min, prior heating of the protease solution stimulats the hydrolysis rate
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE