Information on EC 3.4.22.B67 - Ulp2 peptidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.22.B67
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
Ulp2 peptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Ulp2 hydrolyses the SUMO-SUMO linkage in poly-SUMO chains. It catalyzes desumoylation of SUMO-modified-Ndc10 kinetochore protein, SUMO-modified-Bir1 kinetochore protein or SUMO-modified-Cep3 kinetochore protein.
show the reaction diagram
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
polySUMO-protein conjugate + H2O
SUMO + SUMO-SUMO + SUMO-SUMO-SUMO + truncated polySUMO-protein conjugate
show the reaction diagram
polySUMO-protein conjugate + H2O
SUMO + truncated polySUMO-protein conjugate + H2O
show the reaction diagram
Smt3-modified protein conjugate + H2O
Smt3 + protein
show the reaction diagram
Smt3-precursor + H2O
SMT3 + peptide
show the reaction diagram
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Ulp2 is able to process the Smt3 precursor in vivo, albeit inefficiently relative to Ulp1
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-
?
SUMO-modified-Bir1 kinetochore protein + H2O
SUMO + Bir1 kinetochore protein
show the reaction diagram
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-
-
-
?
SUMO-modified-Cep3 kinetochore protein + H2O
SUMO + Cep3 kinetochore protein
show the reaction diagram
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-
-
-
?
SUMO-modified-Ndc10 kinetochore protein + H2O
SUMO + Ndc10 kinetochore protein
show the reaction diagram
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-
-
-
?
sumoylated human eIF4GI + H2O
?
show the reaction diagram
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translation initiation factor and scaffold protein eIF4G is SUMOylated and associates with the SUMO protease Ulp2, partial co-localisation of recombinant human eIF4G and SUMO in stressed HeLa cells
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
polySUMO-protein conjugate + H2O
SUMO + SUMO-SUMO + SUMO-SUMO-SUMO + truncated polySUMO-protein conjugate
show the reaction diagram
-
-
-
-
?
polySUMO-protein conjugate + H2O
SUMO + truncated polySUMO-protein conjugate + H2O
show the reaction diagram
-
Ulp2 can cleave the SUMO-SUMO linkage in poly-SUMO chains
-
-
?
Smt3-modified protein conjugate + H2O
Smt3 + protein
show the reaction diagram
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Ulp2 is able to cleave isopeptide-linked Smt3 molecules from natural substrates. Smt3-protein deconjugation by Ulp2 is important for normal meiotic development
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-
?
Smt3-precursor + H2O
SMT3 + peptide
show the reaction diagram
-
Ulp2 is able to process the Smt3 precursor in vivo, albeit inefficiently relative to Ulp1
-
-
?
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-ethylmaleimide
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additional information
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no inhibition by phenylmethylsulfonyl fluoride or the Dub-specific inhibitor ubiquitin aldehyde
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant FLAG-tagged and maltose-binding protein-fused isozyme Ulp2, containing a TEV protease recognition site, from Escherichia coli strain BL21 by amylose affinity chromatography
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recombinant TAP-tagged enzyme by affinity chromatography, two translation initiation factors, eIF4G and eIF3h, co-purify with Ulp2 may be through the interaction of Ulp2 with other member(s) of the eIF3 complex
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression as a GST-Ulp2 fusion protein by Escherichia coli
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isozyme Ulp2, recombinant expression of GST-tagged isozyme Ulp2, expression of recombinant FLAG-tagged and maltose-binding protein-fused isozyme Ulp2, containing a TEV protease recognition site, in Escherichia coli strain BL21
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recombinant expression of TAP-tagged enzyme
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C624A
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site-directed mutagenesis, inactive isozyme Ulp2 mutant
H531A
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catalytic-site mutant demonstrates permanent arrest after DNA damage similar to ulp2 deletion mutant
additional information
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immobilization of isozyme Ulp2 via its C-terminal FLAG tag