Information on EC 3.4.22.B65 - cathepsin P

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.22.B65
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
cathepsin P
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of proteins with restricted preference for substrates containing hydrophobic amino acids. Efficiently cleaves -Glu-Ile-Phe-/-Val-Phe-Lys-Gln-. Involved in the processing of endoplasmic proteins
show the reaction diagram
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
94716-08-02
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-(methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 + H2O
7-(methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly + Leu-Dap(Dnp)-Ala-Arg-NH2
show the reaction diagram
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
calreticulin + H2O
?
show the reaction diagram
-
endoplasmic reticulum protein substrate identified by incubation of recombinant enzyme with rat choriocarcinoma cell proteins
protein is processed from the C-terminus, with removal of the KDEL endoplasmic reticulum retention signal. Cathepsin L co-localizes with calreticulin in rat choriocarcinoma cell
-
?
Gp96 protein + H2O
?
show the reaction diagram
-
endoplasmic reticulum protein substrate identified by incubation of recombinant enzyme with rat choriocarcinoma cell proteins
protein is sequentially processed by cathepsin P at 2 sites towards the C-terminus of the protein, with removal of the KDEL endoplasmic reticulum retention signal. Cathepsin L co-localizes with gp96 in rat choriocarcinoma cell
-
?
o-aminobenzoic acid-ALRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-ALR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-EIFVFKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-EIF + VFKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
ideal substrate constructed after analysis of substrate preferences. In contrast to most substrates, hydrolysis is not stimulated by addition of salts
-
-
?
o-aminobenzoic acid-ELRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-ELR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-FLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-FLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-HLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-HLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-ILRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-ILR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KARSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KAR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KFRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KFR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KIRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KIR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLASSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLA + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLFSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLF + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLISSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLI + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine + o-aminobenzoic acid-KLIS + SKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
preferential hydrolysis at the Ser-Ser bond
-
?
o-aminobenzoic acid-KLKSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLK + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLLSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLL + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLMSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLM + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLQSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLQ + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRESKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + ESKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRfSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + fSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRGSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + GSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRHSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + HSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRISKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + ISKQ-N-(2,4-dinitrophenyl)ethylenediamine + o-aminobenzoic acid-KLRIS + KQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
30% cleavage at Arg-Ile bond, 70% cleavage at Ser-Lys bond
-
?
o-aminobenzoic acid-KLRLSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + LSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRNSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + NSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRQSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + QSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRRSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + RSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRVSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + VSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLTSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLT + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLYSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLY + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KMRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KMR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KQRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KQR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KRRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KRR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KTRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KTR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KVRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KVR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KYRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KYR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-LLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-LLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-NLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-NLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-RLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-RLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-VLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-VLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
transferrin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
biotin-(6-hexanamido-hexanoate)2-Tyr-Ala-CHN2
-
-
Cbz-Trp-Trp-4-cyclohexanone-Trp-Phe-OMe
-
reversoble competitive inhibition. 10fold less inhibitory on mouse cathepsin L, 20fold less inhibitory on human cathpsin L, 50fold less inhibotry on human cathepsin B
E-64
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
-
activation
hyaluronate
-
-
Na2SO4
NaBr
-
1-2.5 M, up to 5fold increase in activity
NaCl
-
1-2.5 M, up to 5fold increase in activity
NaH2PO4
-
1-2.5 M, up to 5fold increase in activity
Sodium acetate
-
1-2.5 M, up to 5fold increase in activity
Sodium citrate
-
1-2.5 M, up to 5fold increase in activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
o-aminobenzoic acid-ALRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.15 - 0.5
o-aminobenzoic acid-EIFVFKQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0051
o-aminobenzoic acid-ELRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0094
o-aminobenzoic acid-FLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0048
o-aminobenzoic acid-HLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0085
o-aminobenzoic acid-ILRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0184
o-aminobenzoic acid-KARSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.004
o-aminobenzoic acid-KFRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0069
o-aminobenzoic acid-KIRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0034
o-aminobenzoic acid-KLASSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0015
o-aminobenzoic acid-KLFSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0015
o-aminobenzoic acid-KLLSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0014
o-aminobenzoic acid-KLMSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0029 - 0.013
o-aminobenzoic acid-KLQSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0053
o-aminobenzoic acid-KLRESKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0079
o-aminobenzoic acid-KLRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0058
o-aminobenzoic acid-KLRGSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0153
o-aminobenzoic acid-KLRHSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0046
o-aminobenzoic acid-KLRLSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0202
o-aminobenzoic acid-KLRNSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0084
o-aminobenzoic acid-KLRQSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0107
o-aminobenzoic acid-KLRRSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.004 - 0.01
o-aminobenzoic acid-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0014
o-aminobenzoic acid-KLRVSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.002
o-aminobenzoic acid-KLTSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0038
o-aminobenzoic acid-KLYSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0084
o-aminobenzoic acid-KMRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0048
o-aminobenzoic acid-KNLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0201
o-aminobenzoic acid-KQRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.018
o-aminobenzoic acid-KRRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0243
o-aminobenzoic acid-KTRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0055
o-aminobenzoic acid-KVRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.00044
o-aminobenzoic acid-KYRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.018
o-aminobenzoic acid-LLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.0086
o-aminobenzoic acid-NLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.013
o-aminobenzoic acid-RLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
0.004
o-aminobenzoic acid-VLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
37C, pH 6.5
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.035
o-aminobenzoic acid-ALRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.004 - 0.006
o-aminobenzoic acid-EIFVFKQ-N-(2,4-dinitrophenyl)ethylenediamine
0.021
o-aminobenzoic acid-ELRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.024
o-aminobenzoic acid-FLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.013
o-aminobenzoic acid-HLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.016
o-aminobenzoic acid-ILRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.008
o-aminobenzoic acid-KARSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.004
o-aminobenzoic acid-KFRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.05
o-aminobenzoic acid-KIRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.009
o-aminobenzoic acid-KLASSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.016
o-aminobenzoic acid-KLFSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.02
o-aminobenzoic acid-KLKSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.012
o-aminobenzoic acid-KLLSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.013
o-aminobenzoic acid-KLMSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.006
o-aminobenzoic acid-KLQSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.014
o-aminobenzoic acid-KLRESKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.01
o-aminobenzoic acid-KLRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.007
o-aminobenzoic acid-KLRGSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.009
o-aminobenzoic acid-KLRHSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.018
o-aminobenzoic acid-KLRLSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.044
o-aminobenzoic acid-KLRNSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.029
o-aminobenzoic acid-KLRQSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.018
o-aminobenzoic acid-KLRRSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.0014 - 0.016
o-aminobenzoic acid-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
0.009
o-aminobenzoic acid-KLRVSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.007
o-aminobenzoic acid-KLTSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.015
o-aminobenzoic acid-KLYSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.02
o-aminobenzoic acid-KMRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.016
o-aminobenzoic acid-KNLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.006
o-aminobenzoic acid-KQRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.001
o-aminobenzoic acid-KRRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.013
o-aminobenzoic acid-KTRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.017
o-aminobenzoic acid-KVRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.005
o-aminobenzoic acid-KYRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.029
o-aminobenzoic acid-LLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.012
o-aminobenzoic acid-NLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.023
o-aminobenzoic acid-RLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
0.013
o-aminobenzoic acid-VLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Mus musculus
-
37C, pH 6.5
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000032
Cbz-Trp-Trp-4-cyclohexanone-Trp-Phe-OMe
-
pH 6.5, 37C
0.002
E-64
-
37C, presence of 1.5 M NaSO4
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
-
substrates azocasein and transferrin
7
-
substrate 7-(methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-Dnp(Dpa)-Ala-Arg-NH2
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
little activity below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
Cathepsin P expression is induced during differentiation of Rcho-1 cells into a trophoblast giant cell phenotype
Manually annotated by BRENDA team
-
low level of expression in visceral yolk sac
Manually annotated by BRENDA team
additional information
-
no expression in heart, kidney, liver, lung or spleen
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
cathepsin P co-localizes with cathepsin B and may function in an endosomal-lysosomal compartment
Manually annotated by BRENDA team
-
cathepsin P co-localizes with cathepsin B and may function in an endosomal-lysosomal compartment
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24000
-
x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein
27000
-
x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein
31000
-
x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein
35000
-
x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein
38000
-
x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
treatment of recombinant protein with PNGase F results in a single protein band of 29000 Da in SDS-PAGE for the zymogen and of 22000 Da for the autoactivated protein
proteolytic modification
-
the inactive zymogen can be activated by proteinase K, chymotrypsin or pancreatic elastase at neutral pH. Cathepsin P can be autoactivated at neutral pH, but not at acidic pH
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimethyl sulfoxide
-
increased concentration of dimethyl sulfoxide reduces Vmax but does not affect Km for substrate MOCAc-Pro-Leu-Gly-Leu-A2pr(Dnp)-Ala-Arg-NH2
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Pichia pastoris
-