Information on EC 3.4.22.B47 - legume cysteine endopeptidase

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The expected taxonomic range for this enzyme is: Vigna aconitifolia

EC NUMBER
COMMENTARY hide
3.4.22.B47
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
legume cysteine endopeptidase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
efficient hydrolysis of peptides with Arg in P1 position
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
4.4% of the activity with benzyloxycarbonyl-Phe-Arg-7-(4-methyl)coumarylamide
-
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
10% of the activity with benzyloxycarbonyl-Phe-Arg-7-(4-methyl)coumarylamide
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cystatin
-
reversible inhibition
-
iodoacetamide
-
exceptionally low rat constant for inhibition
iodoacetate
-
exceptionally low rat constant for inhibition
leupeptin
-
reversible inhibition
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00003
Cystatin
-
26°C, pH 6.0
-
0.0000075
leupeptin
-
26°C, pH 6.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
reaction with azocasein
7.5
-
activity with benzyloxycarbonyl-Phe-Arg-7-(4-methyl)coumarylamide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 7.5
-
pH 3.5: about 40% of maximal activity, pH 7.5: about 65% of maximal activity, reaction with casein
4.5 - 8
-
pH 4.5: about 35% of maximal activity, pH 8.0: about 60% of maximal activity, reaction with benzyloxycarbonyl-Phe-Arg-7-(4-methyl)coumarylamide
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6 - 4.7
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
activity increases during germination
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
-
x * 27000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 27000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
the enzyme is not a glycoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 7
-
stable at 26.5°C
30313
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26.5
-
very stable during incubation in the range pH 3.0-7.0
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE