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Information on EC 3.4.22.B29 - calpain 9 and Organism(s) Homo sapiens and UniProt Accession O14815

for references in articles please use BRENDA:EC3.4.22.B29
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B29 calpain 9
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This record set is specific for:
Homo sapiens
UNIPROT: O14815
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
broad endopeptidase specificity
Synonyms
ncl-4, calpain 9, calpain-9, digestive tract-specific calpain, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
calpain-9
-
calpain-9
-
-
CAPN9
-
-
digestive tract-specific calpain
-
-
-
-
nCL-4
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
78990-62-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
peptide + H2O
hydrolyzed peptide
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
regulates the enzyme, contains 2 EF-hand calcium-binding domains
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
calpeptin
-
pan-calpain inhibitor
PD150606
-
pan-calpain inhibitor
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
expression analysis
Manually annotated by BRENDA team
expression analysis
Manually annotated by BRENDA team
suppressed or depleted in gastric cancer cell lines of differentiated and poorly differentiated types
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
calpain-9 may play a role in breast cance, low expression is associated with poorer patient clinical outcome following endocrine therapy, but no associations are observed between calpain-9 expression and clinicopathological variables
malfunction
-
inhibition of CAPN9 expression in MCF7/CEACAM1-4S cells by RNAi or by calpeptin or PD150606 inhibits lumen formation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CAN9_HUMAN
690
0
79097
Swiss-Prot
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, minicalpain 9. The structures of the highly conserved, ubiquitously expressed human calpain 1 and the more tissue specific human calpain 9 indicate that although there are high levels of sequence conservation throughout the calpain family, isolated structures of family members are insufficient to explain the molecular mechanism of activation for this group of proteins
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
low calpain-9 is associated with adverse disease-specific survival following endocrine therapy in breast cancer, but no associations are observed between calpain-9 expression and clinicopathological variables
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Homo sapiens (O14815), Mus musculus (Q9D805), Mus musculus C57/BL6J (Q9D805), Rattus norvegicus (O35920)
Manually annotated by BRENDA team
Lee, H.J.; Sorimachi, H.; Jeong, S.Y.; Ishiura, S.; Suzuki, K.
Molecular cloning and characterization of a novel tissue-specific calpain predominantly expressed in the digestive tract
Biol. Chem.
379
175-183
1998
Homo sapiens (O14815), Homo sapiens, Mus musculus (Q9D805), Mus musculus, Rattus norvegicus (O35920)
Manually annotated by BRENDA team
Yoshikawa, Y.; Mukai, H.; Hino, F.; Asada, K.; Kato, I.
Isolation of two novel genes, down-regulated in gastric cancer
Jpn. J. Cancer Res.
91
459-463
2000
Homo sapiens (O14815), Homo sapiens
Manually annotated by BRENDA team
Davis, T.L.; Walker, J.R.; Finerty, P.J.; Mackenzie, F.; Newman, E.M.; Dhe-Paganon, S.
The crystal structures of human calpains 1 and 9 imply diverse mechanisms of action and auto-inhibition
J. Mol. Biol.
366
216-229
2007
Homo sapiens
Manually annotated by BRENDA team
Chen, C.J.; Nguyen, T.; Shively, J.E.
Role of calpain-9 and PKC-delta in the apoptotic mechanism of lumen formation in CEACAM1 transfected breast epithelial cells
Exp. Cell Res.
316
638-648
2010
Homo sapiens
Manually annotated by BRENDA team
Davis, J.; Martin, S.G.; Patel, P.M.; Green, A.R.; Rakha, E.A.; Ellis, I.O.; Storr, S.J.
Low calpain-9 is associated with adverse disease-specific survival following endocrine therapy in breast cancer
BMC Cancer
14
995
2014
Homo sapiens (O14815)
Manually annotated by BRENDA team