Information on EC 3.4.22.B28 - calpain 8

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.22.B28
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
calpain 8
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
broad endopeptidase specificity
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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endopeptidase; peptides, endopeptidase
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CAS REGISTRY NUMBER
COMMENTARY hide
78990-62-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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calpain 8 is involved in vesicle trafficking between endoplasmic reticulum and Golgi. Calpain 8/nCL-2 is also involved in the mucosal defense against stress-induced gastropathies
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-subunit of the coat protein + H2O
?
show the reaction diagram
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30C, 5 mM Ca2+
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-
?
calpain 8 + H2O
?
show the reaction diagram
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one of the autolysis sites is between Ala5 and Ala6
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-
?
peptide + H2O
hydrolyzed peptide
show the reaction diagram
additional information
?
-
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calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, in which both molecules are essential for activity, and that is involved in gastric mucosal defense. Calpains 8 and 9 are regulated differently from each other and from conventional calpains and, thus, have potentially important, specific functions in the gastrointestinal tract. Calpains 8 and 9 are molecular chaperones for each other, overview
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
peptide + H2O
hydrolyzed peptide
show the reaction diagram
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specific regulatory function of the enzyme in digestive tract
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-
?
additional information
?
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calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, in which both molecules are essential for activity, and that is involved in gastric mucosal defense. Calpains 8 and 9 are regulated differently from each other and from conventional calpains and, thus, have potentially important, specific functions in the gastrointestinal tract. Calpains 8 and 9 are molecular chaperones for each other, overview
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
calpain inhibitor I
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0.010 mM
Calpeptin
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0.010 mM
E64c
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0.050 mM
MG132
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0.010 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
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optimal temperature for nCL-2 activity is 20C. The time courses of nCL-2 activity at various temperatures shows that the initial rates are maximal at 30-37C, whereas maximal activity is achieved at 15C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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high activity
Manually annotated by BRENDA team
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high activity
Manually annotated by BRENDA team
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NS-398 decreases the expression of mRNA for calpain 8. Both NS-398 and indomethacin, but not SC-560, decrease the protein expression of calpains 8
Manually annotated by BRENDA team
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; high activity
Manually annotated by BRENDA team
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diaphragm
Manually annotated by BRENDA team
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gastric
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
oligomer
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stomach-specific calpain, nCL-2/calpain 8, is active without calpain regulatory subunit and oligomerizes through C2-like domains. nCL-2 exists in both monomeric and homo-oligomeric forms, but not as a heterodimer with 30K or 30K-2, and that the oligomerization occurs through domains other than the 5EF-hand domain IV, most probably through domain III
additional information
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enzyme domain structure with prodomain, protease domain, C2-like domain, and 5EF-hand domain
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
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recombinant protein from Escherichia coli
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expression in Escherichia coli
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nCL-2 and nCL-2' are generated by alternative splicing of the same gene Capn8
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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generation of Capn8-/- mice, that are fertile and whose gastric mucosae appears normal. However, the mutant mice are susceptible to gastric mucosal injury induced by ethanol administration, and the Capn8-/- stomach shows significant decreases in both calpains 9 and 8, and the same was true for Capn9-/-. Effects of calpain 8 deficiency on mucous granule formation and secretion, overview