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Information on EC 3.4.22.B25 - calpain 5 and Organism(s) Mus musculus and UniProt Accession O08688

for references in articles please use BRENDA:EC3.4.22.B25
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B25 calpain 5
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This record set is specific for:
Mus musculus
UNIPROT: O08688
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The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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broad endopeptidase specificity
Synonyms
capn5, calpain-5, calpain 5, htra-3, ncl-3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
calpain htra-3
-
-
-
-
htra-3
-
-
-
-
nCL-3
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
78990-62-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
peptide + H2O
hydrolyzed peptide
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
dependent on
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Capn5 is expressed in the ganglion cell, inner nuclear and outer nuclear layers of the retina
Manually annotated by BRENDA team
Calpain-5 mRNA is expressed in the olfactory epithelium. CAPN5 immunoreactivity is ubiquitously detected in the olfactory epithelial layer and lamina propria. Signals are strongly detected in the supporting cells within the epithelium
Manually annotated by BRENDA team
additional information
immunohistochemic analysis, ubiquitous expression in central nerous system cells, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
the enzyme carries dual nuclear localization signals
Manually annotated by BRENDA team
additional information
unlike findings in classical calpains, which are characterized as being mainly cytosolic, CAPN5 is predominantly a noncytosolic calpain, present in the nucleus in the nucleic acidbinding protein fraction. The enzyme is associated with nuclear promyelocytic leukemia protein bodies
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
calpain 5 is a non-classical member of the calpain family. It lacks the EF hand motif characteristic of classical calpains but retains catalytic and Ca2 binding domains, and it contains a unique C-terminal domain
additional information
the enzyme is associated with nuclear promyelocytic leukemia protein bodies
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CAN5_MOUSE
640
0
72955
Swiss-Prot
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene Capn5, DNA and amino acid sequence determination and analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Mus musculus (O08688), Rattus norvegicus (Q8R4C0), Rattus norvegicus Sprague-Dawley (Q8R4C0)
Manually annotated by BRENDA team
Strausberg R.L.; Feingold E.A.; Grouse L.H.; Derge J.G.; et al.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences
Proc. Natl. Acad. Sci. USA
99
16899-16903
2002
Homo sapiens (O15484), Mus musculus (O08688)
Manually annotated by BRENDA team
Dear T.N.; Matena K.; Vingron M.; Boehm T.
A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution
Genomics
45
175-184
1997
Mus musculus (O08688), Mus musculus
Manually annotated by BRENDA team
Matena, K.; Boehm, T.; Dear, N.
Genomic organization of mouse Capn5 and Capn6 genes confirms that they are a distinct calpain subfamily
Genomics
48
117-120
1998
Homo sapiens (O15484), Homo sapiens, Mus musculus (O08688), Mus musculus
Manually annotated by BRENDA team
Singh, R.; Brewer, M.K.; Mashburn, C.B.; Lou, D.; Bondada, V.; Graham, B.; Geddes, J.W.
Calpain 5 is highly expressed in the central nervous system (CNS), carries dual nuclear localization signals, and is associated with nuclear promyelocytic leukemia protein bodies
J. Biol. Chem.
289
19383-19394
2014
Homo sapiens (O15484), Mus musculus (O08688), Mus musculus C57/BL6J (O08688), Rattus norvegicus (Q8R4C0), Rattus norvegicus Sprague-Dawley (Q8R4C0)
Manually annotated by BRENDA team
Schaefer, K.; Mahajan, M.; Gore, A.; Tsang, S.H.; Bassuk, A.G.; Mahajan, V.B.
Calpain-5 gene expression in the mouse eye and brain
BMC Res. Notes
10
602
2017
Mus musculus (O08688), Mus musculus
Manually annotated by BRENDA team
Nakashima, N.; Nakashima, K.; Takaku-Nakashima, A.; Takano, M.
Olfactory receptor neurons express olfactory marker protein but not calpain 5 from the same genomic locus
Mol. Brain
12
54
2019
Mus musculus (O08688)
Manually annotated by BRENDA team