Information on EC 3.4.22.B24 - calpain 4

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.22.B24
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
calpain 4
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
broad endopeptidase specificity
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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endopeptidase; peptides, endopeptidase
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CAS REGISTRY NUMBER
COMMENTARY hide
78990-62-2
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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deletion of calpain 4 leads to reduced chondrocyte proliferation and differentiation in embryonic growth plates. Deletion of calpain 4 in cells of the chondrocyte lineage correlated with impaired cell cycle progression at the G1/S transition, reduced cyclin D gene transcription
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
casein + H2O
?
show the reaction diagram
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-
-
?
cyclin D + H2O
?
show the reaction diagram
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-
-
-
?
p21Cip1 + H2O
?
show the reaction diagram
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-
-
-
?
p27Kip1 + H2O
?
show the reaction diagram
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-
-
-
?
peptide + H2O
hydrolyzed peptide
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cyclin D + H2O
?
show the reaction diagram
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-
-
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?
p21Cip1 + H2O
?
show the reaction diagram
-
-
-
-
?
p27Kip1 + H2O
?
show the reaction diagram
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-
-
-
?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
calpain inhibitor IV
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0.1 mg/ml, irreversible, attacks the active site of m-calpain nucleophilically, the association between calpain 4 and alpha-p21-activated kinase interacting exchange factor (alpha-PIX) is not impaired
Calpeptin
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0.075 mg/ml, reversible, reacts with the active site of both my-calpain and m-calpain, the association between calpain 4 and alpha-p21-activated kinase interacting exchange factor (alpha-PIX) is not impaired
PD 151746
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0.1 mg/ml, reversible, binds predominantly to the Ca2+-binding site of my-calpain, the association between calpain 4 and alpha-p21-activated kinase interacting exchange factor (alpha-PIX) is not impaired
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
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around the nucleus, colocalizes with alpha-p21-activated kinase interacting exchange factor (alpha-PIX)
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
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Capn4 is the common small regulatory subunit of EC 3.4.22.52 and EC 3.4.22.53
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure of calcium-bound domain VI of calpain at 1.9-A resolution
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
HBx is able to upregulate the expression of Capn4 in human hepatoma HepG2 (or H7402) cells
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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Capn4 significantly correlates with invasive phenotype of hepatocellular carcinoma (HCC). Univariate and multivariate analyses indicates that Capn4 is an independent prognostic factor for recurrence and survival of HCC patients