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azocasein + H2O
?
-
-
-
-
?
benzoyl-L-Arg-p-nitroanilide + H2O
benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
L-pyroglutamyl-L-phenylalanyl-L-leucine 4-nitroanilide + H2O
L-pyroglutamyl-L-phenylalanyl-L-leucine + 4-nitroaniline
very low activity
-
-
?
L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide + H2O
?
-
-
-
-
?
L-pyroglutamyl-Phe-Leu-p-nitroanilide + H2O
L-pyroglutamyl-Phe-Leu + p-nitroaniline
-
-
-
-
?
N-acetyl-L-tryptophanamide + H2O
N-acetyl-L-Trp + NH3
N-alpha-benzyloxycarbonyl-Gly-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-Gly + 4-nitrophenol
-
-
-
?
N-alpha-benzyloxycarbonyl-L-Ala-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Ala + 4-nitrophenol
-
-
-
?
N-alpha-benzyloxycarbonyl-L-Asn-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Asn + 4-nitrophenol
-
-
-
?
N-alpha-benzyloxycarbonyl-L-Asp-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Asp + 4-nitrophenol
asclepain cII has highest endoesterolytic activity using N-alpha-benzyloxycarbonyl-amino acid p-nitrophenyl esters with L-Asp as amino acid
-
-
?
N-alpha-benzyloxycarbonyl-L-Gln-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Gln + 4-nitrophenol
-
-
-
?
N-alpha-benzyloxycarbonyl-L-Tyr-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Tyr + 4-nitrophenol
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
N-Benzoyl-L-Arg + ethanol
N-carbobenzoxy-Gly p-nitrophenyl ester + H2O
N-carbobenzoxy-Gly + p-nitrophenol
-
-
-
-
?
N-carbobenzoxy-L-Ala 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Ala + 4-nitrophenol
-
47% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester
-
-
?
N-carbobenzoxy-L-Gln 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Gln + 4-nitrophenol
-
-
-
-
?
N-carbobenzoxy-L-Ile 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Ile + 4-nitrophenol
-
59% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester
-
-
?
N-carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Lys + 4-nitrophenol
-
78% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester
-
-
?
N-carbobenzoxy-L-Tyr 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Tyr + 4-nitrophenol
-
51% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester
-
-
?
N-carbobenzoxy-Leu p-nitrophenyl ester + H2O
N-carbobenzoxy-Leu + p-nitrophenol
-
-
-
-
?
N-carbobenzoxy-Lys p-nitrophenyl ester + H2O
N-carbobenzoxy-Lys + 4-nitrophenol
-
-
-
-
?
N-carbobenzoxy-Tyr p-nitrophenyl ester + H2O
N-carbobenzoxy-Tyr + p-nitrophenol
-
-
-
-
?
N-methanesulfonylglycine 3-bromophenyl ester + H2O
N-methanesulfonylglycine + 3-bromophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-chlorophenyl ester + H2O
N-methanesulfonylglycine + 3-chlorophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-fluorophenyl ester + H2O
N-methanesulfonylglycine + 3-fluorophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-methylphenyl ester + H2O
N-methanesulfonylglycine + 3-methylphenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-nitrophenyl ester + H2O
N-methanesulfonylglycine 3-nitrophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-tert-butylphenyl ester + H2O
N-methanesulfonylglycine + 3-tert-butylphenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-bromophenyl ester + H2O
N-methanesulfonylglycine + 4-bromophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-chlorophenyl ester + H2O
N-methanesulfonylglycine + 4-chlorophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-fluorophenyl ester + H2O
N-methanesulfonylglycine + 4-fluorophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-methylphenyl ester + H2O
N-methanesulfonylglycine + 4-methylphenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-nitrophenyl ester + H2O
N-methanesulfonylglycine + 4-nitrophenol
Asclepias sp.
-
-
-
-
?
Nalpha-carbobenzoxy-Gly-4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
-
about 25% activity compared to Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester
-
-
?
Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-L-Ala + 4-nitrophenol
-
100% activity
-
-
?
Nalpha-carbobenzoxy-L-Gln-4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-L-Gln + 4-nitrophenol
-
about 85% activity compared to Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester
-
-
?
Nalpha-carbobenzoxy-L-Tyr-4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-L-Tyr + 4-nitrophenol
-
about 20% activity compared to Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester
-
-
?
Nalpha-CBZ-L-Gln-4-nitrophenyl ester + H2O
Nalpha-CBZ-L-Gln + 4-nitrophenol
when assayed on N-alpha-CBZ-amino acid-4-nitrophenyl esters, the enzyme exhibits higher preference for the glutamine derivative
-
-
?
oxidized insulin B chain + H2O
?
pGlu-Phe-Leu 4-nitroanilide + H2O
pGlu-Phe-Leu + 4-nitroaniline
-
-
-
?
pGlu-Phe-Leu-p-nitroanilide + H2O
pGlu-Phe-Leu + p-nitroaniline
-
-
-
?
Phe-Val-Asn-Ala-His-Leu-CysA-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + H2O
Phe-Val-Asn + Ser-His-Leu-Val-Glu-Ala-Leu + Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu + Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + Gly-Phe-Phe-Tyr-Thr
-
-
14% Phe-Val-Asn + 22% Ser-His-Leu-Val-Glu-Ala-Leu + 15% Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu + 10% Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + 10% Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + 13% Gly-Phe-Phe-Tyr-Thr
?
soybean protein + H2O
?
the enzyme is able to selectively hydrolyze soybean proteins at pH 10, employing an enzyme/substrate ratio of 0.2% (w/w). The enzymatic hydrolysis allows a strong increase in the solubility, water and oil holding capacity
-
-
?
Z-Arg-p-nitroanilide + H2O
Z-Arg + p-nitroaniline
-
-
-
-
?
additional information
?
-
casein + H2O
?
-
-
-
?
casein + H2O
?
-
about 50% degradation after 6 h
-
-
?
N-acetyl-L-tryptophanamide + H2O
N-acetyl-L-Trp + NH3
-
-
-
-
?
N-acetyl-L-tryptophanamide + H2O
N-acetyl-L-Trp + NH3
-
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
N-Benzoyl-L-Arg + ethanol
-
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
N-Benzoyl-L-Arg + ethanol
-
-
-
-
?
oxidized insulin B chain + H2O
?
Asclepias sp.
-
detection of cleavage points
-
-
?
oxidized insulin B chain + H2O
?
12 cut-off points
-
-
?
additional information
?
-
-
asclepain cI is the main active enzyme, but shows lower specific activity than asclepain cII, which is the minor proteolytic component in the latex, overview
-
-
?
additional information
?
-
asclepain cI is the main active enzyme, but shows lower specific activity than asclepain cII, which is the minor proteolytic component in the latex, overview
-
-
?
additional information
?
-
asclepain cI is the main active enzyme, but shows lower specific activity than asclepain cII, which is the minor proteolytic component in the latex, overview
-
-
?
additional information
?
-
-
asclepain cII is the minor proteolytic component in the latex, but shows higher specific activity than asclepain cI, the main active enzyme, overview
-
-
?
additional information
?
-
asclepain cII is the minor proteolytic component in the latex, but shows higher specific activity than asclepain cI, the main active enzyme, overview
-
-
?
additional information
?
-
asclepain cII is the minor proteolytic component in the latex, but shows higher specific activity than asclepain cI, the main active enzyme, overview
-
-
?
additional information
?
-
-
the peptide PFLNA is a poor substrate for asclepain cII
-
-
?
additional information
?
-
the peptide PFLNA is a poor substrate for asclepain cII
-
-
?
additional information
?
-
the peptide PFLNA is a poor substrate for asclepain cII
-
-
?
additional information
?
-
-
no activity towards N-alpha-benzyloxycarbonyl-L-Val-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Leu-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Ile-4-nitrophenyl ester, and N-alpha-benzyloxycarbonyl-L-Lys-4-nitrophenyl ester
-
-
?
additional information
?
-
no activity towards N-alpha-benzyloxycarbonyl-L-Val-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Leu-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Ile-4-nitrophenyl ester, and N-alpha-benzyloxycarbonyl-L-Lys-4-nitrophenyl ester
-
-
?
additional information
?
-
no activity towards N-alpha-benzyloxycarbonyl-L-Val-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Leu-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Ile-4-nitrophenyl ester, and N-alpha-benzyloxycarbonyl-L-Lys-4-nitrophenyl ester
-
-
?
additional information
?
-
-
ascpelain f shows low milk clotting activity
-
-
?
additional information
?
-
-
no activity towards Nalpha-carbobenzoxy-L-Leu-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Asn-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Trp-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Val-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Asp-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-lys-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Phe-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Pro-4-nitrophenyl ester, and Nalpha-carbobenzoxy-L-Ile-4-nitrophenyl ester
-
-
?
additional information
?
-
the cut specificity is governed by the presence of hydrophobic residues (F, L, V) in the P2 position
-
-
?
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Lynn, K.R.; Clevette-Radford, N.A.
A comparison of esterolytic mechanism of several sulfhydryl proteases
Bioorg. Chem.
11
96-100
1982
Asclepias sp.
-
brenda
Lynn K.R.; Brockbank, W.J.; Clevette, N.A.
Multiple forms of the asclepains. Cysteinyl proteases from milkweed
Biochim. Biophys. Acta
612
119-125
1980
Asclepias syriaca
brenda
Brockbank, W.J.; Lynn, K.R.
Purification and preliminary characterization of two asclepains from the latex of Asclepias syriaca L. (milkweed)
Biochim. Biophys. Acta
578
13-22
1979
Asclepias syriaca
brenda
Greenberg, D.M.
Plant proteolytic enzymes
Methods Enzymol.
2
54-64
1955
Asclepias mexicana, Asclepias speciosa, Asclepias syriaca
-
brenda
Tablero, M.; Arreguin, R.; Arreguin, B.; Soriano, M.; Sanchez, R.I.; Romero, A.R.; Hernandez-Arana, A.
Purification and characterization of multiple forms of asclepain g from Asclepias glaucescens H.B.K.
Plant Sci.
74
7-15
1991
Asclepias glaucescens, Asclepias glaucescens H.B.K
-
brenda
Lynn, K.R.
Definition of the site of reactivity of the ancestral protease of the papain type
Phytochemistry
22
2485-2487
1983
Asclepias sp.
-
brenda
Liggieri, C.; Arribere, M.C.; Trejo, S.A.; Canals, F.; Aviles, F.X.; Priolo, N.S.
Purification and biochemical characterization of asclepain c I from the latex of Asclepias curassavica L
Protein J.
23
403-411
2004
Asclepias curassavica
brenda
Liggieri, C.; Obregon, W.; Trejo, S.; Priolo, N.
Biochemical analysis of a papain-like protease isolated from the latex of Asclepias curassavica L
Acta Biochim. Biophys. Sin. (Shanghai)
41
154-162
2009
Asclepias curassavica, Asclepias curassavica (B7VF64), Asclepias curassavica (B7VF65)
brenda
Obregon, W.D.; Liggieri, C.S.; Trejo, S.A.; Aviles, F.X.; Vairo-Cavalli, S.E.; Priolo, N.S.
Characterization of papain-like isoenzymes from latex of Asclepias curassavica by molecular biology validated by proteomic approach
Biochimie
91
1457-1464
2009
Asclepias curassavica (B7VF64), Asclepias curassavica (B7VF65), Asclepias curassavica
brenda
Morcelle, S.; Liggieri, C.; Bruno, M.; Priolo, N.; Claps, P.
Screening of plant peptidases for the synthesis of arginine-based surfactants
J. Mol. Catal. B
57
177-182
2009
Asclepias curassavica
-
brenda
Trejo, S.A.; Lopez, L.M.; Caffini, N.O.; Natalucci, C.L.; Canals, F.; Aviles, F.X.
Sequencing and characterization of asclepain f: the first cysteine peptidase cDNA cloned and expressed from Asclepias fruticosa latex
Planta
230
319-328
2009
Gomphocarpus fruticosus subsp. fruticosus (B5BLP0)
brenda
Pardo, M.; Bruno, M.; Sequeiros, C.; Trejo, S.; Lpez, L.; Caffini, N.; Natalucci, C.
New plant endopeptidases with potential application in cheesemaking
Acta Aliment.
39
211-221
2010
Gomphocarpus fruticosus
-
brenda
Karpagam, N.; Viswanathan, S.; Prabhu, S.; Somasundaram, S.; Sivanandham, M.
Biochemical and In silico clotting activity of latex from Asclepias curassavica. L
Int. J. Pharm. Bio Sci.
4
B542-B552
2013
Asclepias curassavica (B7VF65)
-
brenda
Torres, M.J.; Natalucci, C.; Lopez, L.M.I.; Trejo, S.A.
Insights into the hydrolytic activity of Asclepias fruticosa L. protease
Biotechnol. Lett.
41
1043-1050
2019
Gomphocarpus fruticosus subsp. fruticosus (B5BLP0)
brenda