Information on EC 3.4.22.67 - zingipain

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The expected taxonomic range for this enzyme is: Zingiber

EC NUMBER
COMMENTARY
3.4.22.67
-
RECOMMENDED NAME
GeneOntology No.
zingipain
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
preferential cleavage of peptides with a proline residue at the P2 position
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C01.017
-
-
-
-
cysteine proteinase GP-II
-
-
-
-
F50
Zingiber ottensii Valeton
-
-
-
ginger protease
-
-
ginger protease II
-
-
GP-II
-
-
-
-
zingibain
-
-
CAS REGISTRY NUMBER
COMMENTARY
246044-91-7
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Zingiber ottensii Valeton
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
zingipain shows antiproliferative activities against fungi, i.e. Fusarium oxysporum, Exserohilum turicicum, and Collectotrichum cassicola, and human malignant cell lines, e.g. Hep-G2 and SW-620, although not against bacterial cells
physiological function
Zingiber ottensii Valeton
-
zingipain shows antiproliferative activities against fungi, i.e. Fusarium oxysporum, Exserohilum turicicum, and Collectotrichum cassicola, and human malignant cell lines, e.g. Hep-G2 and SW-620, although not against bacterial cells
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetoacetate decarboxylase + H2O
?
show the reaction diagram
-
-
-
-
?
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
azocasein + H2O
?
show the reaction diagram
Zingiber ottensii, Zingiber ottensii Valeton
-
-
-
-
?
Bovine serum albumin + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
Collagen + H2O
?
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
Myoglobin + H2O
?
show the reaction diagram
-
-
-
-
?
Lysozyme + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
enzyme prefers to cleave peptide bonds at which Pro is in the P2 position
-
-
-
additional information
?
-
P82474
enzyme prefers to cleave peptide bonds at which Pro is in the P2 position
-
-
-
additional information
?
-
-
initial cleavage is at peptide bonds with P2 Pro
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
iodoacetic acid
-
-
mercurial
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Zingiber ottensii Valeton
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
34800
-
SDS-PAGE
669676
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 13800 + x * 15200 + x * 32500, SDS-PAGE
?
Zingiber ottensii Valeton
-
x * 13800 + x * 15200 + x * 32500, SDS-PAGE
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
-
two N-linked oligosaccharide chains, 8% by weight, at Asn99 and Asn156
glycoprotein
P82474
Asn99 and Asn156 are occupied by the glycans (Man)3(xyl)1(Fuc)1(GlcNAc)2 and (Man)3(Xyl)1(Fuc)1(GlcNAc)3 in a ratio of 7:1. Both glycan s are xylose containing biantennary complex types that share the common core structural unit, Man1-6(Man1-3)(Xyl1-2)Man1-4GlcNAc1-4(Fuc1-3)GlcNAc
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
at 2.1 A resolution
-
vapor difusion method with hanging-drop geometry
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5
-
half-life of 2.1 d decreasing to 20 min at 30°C, addition of ascorbate increases the half-time to 20 d, acetone powder preperations from ginger yielded a half-time of 18 months
669676
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially purified 252fold with a recovery of 61%, ion exchange chromatography
-
native enzyme from rhizomes by ammonium sulfate fractionation, anion exchange chromatography, SDS-PAGE, and gel filtration
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
nutrition
-
meat tenderizing agent, stability of the enzyme can be greatly improved, increasing its attractiveness as a commercial product