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Information on EC 3.4.22.53 - calpain-2 and Organism(s) Oryctolagus cuniculus and UniProt Accession P06814

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.53 calpain-2
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This record set is specific for:
Oryctolagus cuniculus
UNIPROT: P06814 not found.
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Word Map
The taxonomic range for the selected organisms is: Oryctolagus cuniculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
broad endopeptidase specificity
Synonyms
m-calpain, calpain ii, calpain-2, calpain 2, calpain2, ncl-2, milli-calpain, mitochondrial m-calpain, rat calpain 2, calcium-activated neutral protease ii, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
calcium-activated neutral protease II
-
-
-
-
calpain 2
-
-
-
-
calpain II
-
-
-
-
calpain xCL-2 (Xenopus leavis)
-
-
-
-
CAPN2 g.p. (Homo sapiens)
-
-
-
-
m-calpain
-
-
-
-
milli-calpain
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
702693-80-9
-
78990-62-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Boc-Leu-Met-7-amino-4-chloromethylcoumarin
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
Fibronectin + H2O
?
show the reaction diagram
-
-
-
-
?
IkappaBalpha + H2O
?
show the reaction diagram
succinyl-bovine serum albumin + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-bovine-serum-albumin + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-casein + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-insulin B + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-protamine + H2O
?
show the reaction diagram
-
-
-
-
?
talin + H2O
?
show the reaction diagram
-
-
-
-
?
desmin + H2O
additional information
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
IkappaBalpha + H2O
?
show the reaction diagram
-
a parallel pathway that degrades IkappaBalpha and activates NF-kappaB activation independently of the ubiquitin-proteasome pathway
-
-
?
additional information
?
-
-
the enzyme is involved in myoblast fusion by cleaving certain proteins. This cleavage could modify membrane and cytoskeleton organization for the myoblast to fuse
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Sr2+
-
activates, Ka: 5.1 mM
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
antipain
-
0.01 mM, 80-90% inhibition
benzyloxycarbonyl-Leu-Leu-leucinal
-
-
benzyloxycarbonyl-Leu-Leu-phenylalaninal
-
-
benzyloxycarbonyl-Leu-Leu-Tyr diazomethyl ketone
-
-
benzyloxycarbonyl-Leu-norleucinal
-
-
E-64
-
i.e. trans-epoxysuccinyl-L-leucylamido-(4-guanido)butane
E-64c
-
0.01 mM, 80-90% inhibition
leupeptin
-
0.01 mM, 80-90% inhibition
pepstatin A
-
1 mM, 60-80% inhibition
TLCK
-
0.1 mM, 60-80% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
TNF-alpha
-
activates cytosolic enzyme
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.2
succinyl-bovine serum albumin
-
pH 7.5, 25°C
-
10
succinyl-casein
-
pH 7.5, 25°C
-
453.7
succinyl-insulin B
-
pH 7.5, 25°C
-
101.3
succinyl-protamine
-
pH 7.5, 25°C
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CAN2_RABIT
422
0
49494
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
-
1 * 80000 + 1 * 28000, SDS-PAGE
80000
-
1 * 80000 + 1 * 28000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
1 * 80000 + 1 * 28000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
10 min, complete loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
E-F hand structure-domains
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA fragments corresponding to the domains with E-F hand structures in the large and small subunits are inserted into an expression vector pIC18 or pUC8. The resulting plasmids are used to transform Escherichia coli and isopropyl-1-thio-beta-D-galactoside-inducible expression is induced
isolation of cDNA clone for the 80000 Da subunit
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Emori, Y.; Kawasaki, H.; Sugihara, H.; Imajoh, S.; Kawashima, S.; Suzuki K.
Isolation and sequence analyses of cDNA clones for the large subunits of two isozymes of rabbit calcium-dependent protease
J. Biol. Chem.
261
9465-9471
1986
Oryctolagus cuniculus (P06814)
Manually annotated by BRENDA team
Minami, Y.; Emori, Y.; Kawasaki, H.; Suzuki, K.
E-F hand structure-domain of calcium-activated neutral protease (CANP) can bind Ca2+ ions
J. Biochem.
101
889-895
1987
Oryctolagus cuniculus (P06814)
Manually annotated by BRENDA team
Penny, I.F.; Taylor, M.A.J.; Harris, A.G.; Etherington, D.J.
Purification and immunological characterization of two calcium-activated neutral proteinases from rabbit skeletal muscle
Biochim. Biophys. Acta
829
244-252
1985
Oryctolagus cuniculus
Manually annotated by BRENDA team
Inomata, M.; Nomoto, M.; Hayashi, M.; Nakamura, M.; Imahori, K.; Kawashima, S.
Comparison of low and high calcium requiring forms of the calcium-activated neutral protease (CANP) from rabbit skeletal muscle
J. Biochem.
95
1661-1670
1984
Oryctolagus cuniculus
Manually annotated by BRENDA team
Dourdin, N.; Balcerzak, D.; Brustis, J.J.; Poussard, S.; Cottin, P.; Ducastaing, A.
Potential m-calpain substrates during myoblast fusion
Exp. Cell Res.
246
433-442
1999
Oryctolagus cuniculus
Manually annotated by BRENDA team
Han, Y.; Weinman, S.; Boldogh, I.; Walker, R.K.; Brasier, A.R.
Tumor necrosis factor-a-inducible I.kappa.Ba proteolysis mediated by cytosolic m-calpain. A mechanism parallel to the ubiquitin-proteasome pathway for nuclear factor-kB activation
J. Biol. Chem.
274
787-794
1999
Oryctolagus cuniculus
Manually annotated by BRENDA team