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Information on EC 3.4.22.53 - calpain-2 and Organism(s) Mus musculus and UniProt Accession O08529

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.53 calpain-2
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This record set is specific for:
Mus musculus
UNIPROT: O08529 not found.
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
broad endopeptidase specificity
Synonyms
m-calpain, calpain ii, calpain-2, calpain 2, calpain2, ncl-2, milli-calpain, mitochondrial m-calpain, rat calpain 2, calcium-activated neutral protease ii, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CAPN2
catalytic subunit of m-calpain
calcium-activated neutral protease II
-
-
-
-
calpain 2
calpain II
-
-
-
-
calpain xCL-2 (Xenopus leavis)
-
-
-
-
calpain-2
-
-
CAPN2 g.p. (Homo sapiens)
-
-
-
-
m-calpain
milli-calpain
-
-
-
-
nCL-2
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
702693-80-9
-
78990-62-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
striatal-enriched protein tyrosine phosphatase + H2O
?
show the reaction diagram
beta-subunit of coatomer complex beta-COP + H2O
?
show the reaction diagram
-
-
-
-
?
Boc-Leu-Met-7-amido-4-chloromethylcoumarin + H2O
Boc-Leu-Met + 7-amino-4-chloromethylcoumarin
show the reaction diagram
-
10 microM, 20 min, 37 °C, with or without magnetic bead stimulation
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
crystallin + H2O
?
show the reaction diagram
tert-butoxycarbonyl-Leu-Met-7-amido-4-chloromethylcoumarin + H2O
tert-butoxycarbonyl-Leu-Met + 7-amino-4-chloromethylcoumarin
show the reaction diagram
-
-
-
-
?
upstream stimulatory factor + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
striatal-enriched protein tyrosine phosphatase + H2O
?
show the reaction diagram
calpain-2 activation cleaves striatal-enriched protein tyrosine phosphatase and activates STEP-mediated pro-death pathway in retinal ganglion cells after intraocular pressure elevation
-
-
?
crystallin + H2O
?
show the reaction diagram
-
alphaA crystallin in lenses from wild-type mice is proteolyzed by both calpain 2 and Lp82. Crystallins proteolyzed by calpain Lp82 are more susceptible to insolubilization than crystallins proteolyzed by calpain 2
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
dependent on
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
benzyloxycarbonyl-Leu-Abu-CONH-CH2-C6H3(3,5-(OMe)2)
calpain-2 selective inhibitor. A selective calpain-2 inhibitor could prevent acute glaucoma-induced retinal ganglion cell death and blindness
ALLM
-
-
calpastatin
-
calpeptin
-
-
indomethacin
-
-
N-acetyl-L-leucyl-L-leucyl-L-methioninal
-
-
NS-398
-
-
SC-560
-
-
additional information
-
suppression of calpain-2 expression with adenovirus vector-mediated RNAi
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
increase of m-calpain expression by mechanical stimulation via laminin receptors
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
20 min, with or without magnetic bead stimulation
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
isoform-specific hyperactivation of calpain-2, but not calpain-1 occurs at the synapse early in the pathogenesis of Alzheimer's disease potentially contributing to the deregulation of synaptic signaling in Alzheimer's disease
Manually annotated by BRENDA team
the large catalytic subunits of calpains 2 and the small regulatory subunit common to calpains 1 and 2 are weakly expressed in the parotid gland, sublingual gland, and submandibular gland at similar levels in males and females
Manually annotated by BRENDA team
the large catalytic subunits of calpains 2 and the small regulatory subunit common to calpains 1 and 2 are weakly expressed in the parotid gland, sublingual gland, and submandibular gland at similar levels in males and females
Manually annotated by BRENDA team
the large catalytic subunits of calpains 2 and the small regulatory subunit common to calpains 1 and 2 are weakly expressed in the parotid gland, sublingual gland, and submandibular gland at similar levels in males and females
Manually annotated by BRENDA team
-
relatively high m-calpain expression levels in the inferior arch
Manually annotated by BRENDA team
-
nCL-2 is localized strictly to the surface cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum
Manually annotated by BRENDA team
-
analysis from E8.5 to 14.5 stages indicates high levels of capn2 expression in the nervous system, heart and mesodermal tissues. Up-regulation is maintained during later developmental stages in proliferating cells and in precursor cells involved in muscle (myoblasts) or bone formation (chondrocytes). At later developmental stages, elevated mRNA levels coincide with CAPN2 nuclear localization in these cell types, while differentiated cells maintain cytoplasmic expression
Manually annotated by BRENDA team
-
calpain 2 is first expressed late in embryonic development and localizes to the lens epithelium and transition zone
Manually annotated by BRENDA team
-
nCL-2 is localized strictly to the surface cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
from ES cells and the 8-cell stage to late neurulation stages, CAPN2 is expressed in the cytoplasm and nuclear compartments
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
isoform-specific hyperactivation of calpain-2, but not calpain-1 occurs at the synapse early in the pathogenesis of Alzheimer's disease potentially contributing to the deregulation of synaptic signaling in Alzheimer's disease
physiological function
calpain-1 and calpain-2 play opposite roles in retinal ganglion cell death induced by acute intraocular pressure elevation. Calpain-1 activity supports survival of retinal ganglion cell after intraocular pressure elevation. Calpain-2 activity promotes cell death of retinal ganglion cells after intraocular pressure elevation. Calpain-2 activation cleaves striatal-enriched protein tyrosine phosphatase and activates STEP-mediated pro-death pathway in retinal ganglion cells after intraocular pressure elevation
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CAN2_MOUSE
700
0
79872
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
-
active form, SDS-PAGE
80000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 80000, SDS-PAGE
heterodimer
-
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of mutated calpain 2 C105A is driven in lens by coupling the mutated gene to the betaB1-crystallin promoter
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
calpain 2 expression levels increase throughout stem cell differentiation
-
calpain 2 is upregulated in mouse pancreas exposed to caerulein for 12 h
-
m-calpain expression levels in aorta in siRNAtreated mice are significantly diminished by 63.5% in comparison to those in control RNA-transfected mice with no alteration of m-calpain or beta-actin expression
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
a selective calpain-2 inhibitor could prevent acute glaucoma-induced retinal ganglion cell death and blindness
medicine
-
calpain 2 represents a potential therapeutic target in breast cancer
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dear T.N.; Matena K.; Vingron M.; Boehm T.
A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution
Genomics
45
175-184
1997
Mus musculus (O08529), Mus musculus
Manually annotated by BRENDA team
Glading, A.; Chang, P.; Lauffenburger, D.A.; Wells, A.
Epidermal growth factor receptor activation of calpain is required for fibroblast motility and occurs via an ERK/MAP kinase signaling pathway
J. Biol. Chem.
275
2390-2398
2000
Mus musculus
Manually annotated by BRENDA team
Hata, S.; Koyama, S.; Kawahara, H.; Doi, N.; Maeda, T.; Toyama-Sorimachi, N.; Abe, K.; Suzuki, K.; Sorimachi, H.
Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP
J. Biol. Chem.
281
11214-11224
2006
Mus musculus
Manually annotated by BRENDA team
Azuma, M.; Tamada, Y.; Kanaami, S.; Nakajima, E.; Nakamura, Y.; Fukiage, C.; Forsberg, N.E.; Duncan, M.K.; Shearer, T.R.
Differential influence of proteolysis by calpain 2 and Lp82 on in vitro precipitation of mouse lens crystallins
Biochem. Biophys. Res. Commun.
307
558-563
2003
Mus musculus
Manually annotated by BRENDA team
Franco, S.; Perrin, B.; Huttenlocher, A.
Isoform specific function of calpain 2 in regulating membrane protrusion
Exp. Cell Res.
299
179-187
2004
Mus musculus
Manually annotated by BRENDA team
Reed, N.A.; Castellini, M.A.; Ma, H.; Shearer, T.R.; Duncan, M.K.
Protein expression patterns for ubiquitous and tissue specific calpains in the developing mouse lens
Exp. Eye Res.
76
433-443
2003
Mus musculus
Manually annotated by BRENDA team
Shao, H.; Chou, J.; Baty, C.J.; Burke, N.A.; Watkins, S.C.; Stolz, D.B.; Wells, A.
Spatial localization of m-calpain to the plasma membrane by phosphoinositide biphosphate binding during epidermal growth factor receptor-mediated activation
Mol. Cell. Biol.
26
5481-5496
2006
Mus musculus
Manually annotated by BRENDA team
Dutt, P.; Croall, D.E.; Arthur, J.S.; De Veyra, T.; Williams, K.; Elce, J.S.; Greer, P.A.
m-Calpain is required for preimplantation embryonic development in mice
BMC Dev. Biol.
6
3
2006
Mus musculus (O08529), Mus musculus
Manually annotated by BRENDA team
Raynaud, F.; Marcilhac, A.; Chebli, K.; Benyamin, Y.; Rossel, M.
Calpain 2 expression pattern and sub-cellular localization during mouse embryogenesis
Int. J. Dev. Biol.
52
383-388
2008
Mus musculus
Manually annotated by BRENDA team
Honda, M.; Masui, F.; Kanzawa, N.; Tsuchiya, T.; Toyo-oka, T.
Specific knockdown of m-calpain blocks myogenesis with cDNA deduced from the corresponding RNAi
Am. J. Physiol. Cell Physiol.
294
C957-C965
2008
Mus musculus
Manually annotated by BRENDA team
Grossi, A.; Karlsson, A.H.; Lawson, M.A.
Mechanical stimulation of C2C12 cells increases m-calpain expression, focal adhesion plaque protein degradation
Cell Biol. Int.
32
615-622
2008
Mus musculus
Manually annotated by BRENDA team
Nakada, S.; Tsuneyama, K.; Kato, I.; Tabuchi, Y.; Takasaki, I.; Furusawa, Y.; Kawaguchi, H.; Fujimoto, M.; Goto, H.; Hikiami, H.; Kondo, T.; Takano, Y.; Shimada, Y.
Identification of candidate genes involved in endogenous protection mechanisms against acute pancreatitis in mice
Biochem. Biophys. Res. Commun.
391
1342-1347
2010
Mus musculus
Manually annotated by BRENDA team
Miyazaki, T.; Honda, K.; Ohata, H.
m-Calpain antagonizes RhoA overactivation and endothelial barrier dysfunction under disturbed shear conditions
Cardiovasc. Res.
85
530-541
2010
Mus musculus
Manually annotated by BRENDA team
Magnaghi-Jaulin, L.; Marcilhac, A.; Rossel, M.; Jaulin, C.; Benyamin, Y.; Raynaud, F.
Calpain 2 is required for sister chromatid cohesion
Chromosoma
119
267-274
2010
Mus musculus
Manually annotated by BRENDA team
Arroba, A.I.; Wallace, D.; Mackey, A.; de la Rosa, E.J.; Cotter, T.G.
IGF-I maintains calpastatin expression and attenuates apoptosis in several models of photoreceptor cell death
Eur. J. Neurosci.
30
975-986
2009
Mus musculus
Manually annotated by BRENDA team
Lin, I.J.; Zhou, Z.; Crusselle-Davis, V.J.; Moghimi, B.; Gandhi, K.; Anantharaman, A.; Pantic, D.; Huang, S.; Jayandharan, G.; Zhong, L.; Srivastava, A.; Bungert, J.
Calpeptin increases the activity of upstream stimulatory factor and induces high level globin gene expression in erythroid cells
J. Biol. Chem.
284
20130-20135
2009
Mus musculus
Manually annotated by BRENDA team
Silver, K.; Leloup, L.; Freeman, L.C.; Wells, A.; Lillich, J.D.
Non-steroidal anti-inflammatory drugs inhibit calpain activity and membrane localization of calpain 2 protease
Int. J. Biochem. Cell Biol.
42
2030-2036
2010
Mus musculus
Manually annotated by BRENDA team
Ho, W.C.; Pikor, L.; Gao, Y.; Elliott, B.E.; Greer, P.A.
Calpain 2 regulates Akt-FoxO-p27(Kip1) protein signaling pathway in mammary carcinoma
J. Biol. Chem.
287
15458-15465
2012
Mus musculus
Manually annotated by BRENDA team
Santos, D.M.; Xavier, J.M.; Morgado, A.L.; Sola, S.; Rodrigues, C.M.
Distinct regulatory functions of calpain 1 and 2 during neural stem cell self-renewal and differentiation
PLoS ONE
7
e33468
2012
Mus musculus
Manually annotated by BRENDA team
Kumchantuek, T.; Nakata, H.; Sakulsak, N.; Yamamoto, M.; Iseki, S.
Expression and localization of calpain 3 in the submandibular gland of mice
Arch. Oral Biol.
70
9-15
2016
Mus musculus (O08529)
Manually annotated by BRENDA team
Wang, Y.; Lopez, D.; Davey, P.G.; Cameron, D.J.; Nguyen, K.; Tran, J.; Marquez, E.; Liu, Y.; Bi, X.; Baudry, M.
Calpain-1 and calpain-2 play opposite roles in retinal ganglion cell degeneration induced by retinal ischemia/reperfusion injury
Neurobiol. Dis.
93
121-128
2016
Mus musculus (O08529)
Manually annotated by BRENDA team
Ahmad, F.; Das, D.; Kommaddi, R.P.; Diwakar, L.; Gowaikar, R.; Rupanagudi, K.V.; Bennett, D.A.; Ravindranath, V.
Isoform-specific hyperactivation of calpain-2 occurs presymptomatically at the synapse in Alzheimers disease mice and correlates with memory deficits in human subjects
Sci. Rep.
8
13119
2018
Mus musculus (O08529), Mus musculus
Manually annotated by BRENDA team