Information on EC 3.4.22.50 - V-cath endopeptidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Baculoviridae

EC NUMBER
COMMENTARY
3.4.22.50
-
RECOMMENDED NAME
GeneOntology No.
V-cath endopeptidase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
endopeptidase of broad specificity, hydrolyzing substrates of both cathepsin L and cathepsin B
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AcNPV protease
-
-
-
-
baculovirus cathepsin
-
-
-
-
BmNPV protease
-
-
-
-
cathepsin
Cydia pomonella granulovirus CpGV-M1
-
-
-
cysteine protease
-
-
NPV protease
-
-
-
-
nucleopolyhedrosis virus protease
-
-
-
-
V-cath
-
-
V-cath
Cydia pomonella granulovirus CpGV-M1
-
-
-
viral cathepsin
-
-
-
-
viral cysteine protease
-
-
viral cysteine protease
Cydia pomonella granulovirus CpGV-M1
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
316365-69-2
-
9001-92-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
in conjunction with chitinase, the enzyme promotes liquefaction of the host in the latter stages of infection
SwissProt
Manually annotated by BRENDA team
baculovirus, enzyme may aid in the degradation of host proteins as a source of amino acids for viral protein production
SwissProt
Manually annotated by BRENDA team
sruce budworm virus
-
-
Manually annotated by BRENDA team
Cydia pomonella granulovirus CpGV-M1
CpGV-M1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
azocasein + H2O
?
show the reaction diagram
P25783
-
-
-
?
Azocoll + H2O
?
show the reaction diagram
P41721
-
-
-
?
Azocoll + H2O
?
show the reaction diagram
Cydia pomonella granulovirus, Cydia pomonella granulovirus CpGV-M1
-
-
-
-
?
N-benzyloxycarbonyl-Arg-Arg-7-amino-4-methylcoumarin + H2O
?
show the reaction diagram
P25783
-
-
-
?
N-benzyloxycarbonyl-Leu-Arg-7-amino-4-methylcoumarin + H2O
?
show the reaction diagram
P25783
-
-
-
?
N-benzyloxycarbonyl-Phe-Arg-7-amino-4-methylcoumarin + H2O
?
show the reaction diagram
P25783
-
-
-
?
N-benzyloxycarbonyl-Val-Arg-7-amino-4-methylcoumarin + H2O
?
show the reaction diagram
P25783
-
-
-
?
N-benzyloxycarbonyl-Val-Val-Arg-7-amino-4-methylcoumarin + H2O
?
show the reaction diagram
P25783
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
antipain
P25783
0.00005 mM, complete inhibition
Chicken cystatin
P25783
0.0001 mM, complete inhibition
-
E64
P41721
cysteine protease specific inhibitor
E64
P25783
0.0001 mM, complete inhibition
iodoacetate
P25783
0.05 mM, 80% inhibition
Leupeptin
P25783
strong inhibition
Leupeptin
P25783
0.00005 mM, complete inhibition
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
-
10 microM
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Urea
P25783
3 M, enhances activity
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.003
-
Z-Arg-Arg-7-amido-4-methylcoumarin
P25783
-
0.022
-
Z-Leu-Arg-7-amino-4-methylcoumarin
P25783
-
0.039
-
Z-Phe-Arg-7-amino-4-methylcoumarin
P25783
-
0.043
-
Z-Val-Arg-7-amino-4-methylcoumarin
P25783
-
0.058
-
Z-Val-Val-Arg-7-amino-4-methylcoumarin
P25783
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.73
-
Z-Arg-Arg-7-amido-4-methylcoumarin
P25783
-
0.73
-
Z-Leu-Arg-7-amino-4-methylcoumarin
P25783
-
0.95
-
Z-Phe-Arg-7-amino-4-methylcoumarin
P25783
-
0.94
-
Z-Val-Arg-7-amino-4-methylcoumarin
P25783
-
0.51
-
Z-Val-Val-Arg-7-amino-4-methylcoumarin
P25783
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
5.5
P25783
bell-shaped profile, hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin and Z-Arg-Arg-7-amino-4-methylcoumarin
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3
7.5
P25783
hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin
4
7.5
P25783
hydrolysis of Z-Arg-Arg-7-amino-4-methylcoumarin
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
P25783
x * 27500, 35500 Da and 32000 Da species are probaly precursors of the mature enzyme, immunoblot; x * 37000, deduced from nucleotide sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
P25783
N-glycosylation may occur at Asn158
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Sf9 insect cells
P25783
sequencing of BmNPV
P41721
expression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
in this study a cysteine protease-deleted Bombyx mori multiple nucleopolyhedrovirus (CPD-BmMNPV) bacmid using the lambda recombination system is constructed. The protease activities of Bombyx mori cells and silkworm larvae infected with this CPD-BmMNPV bacmid are reduced by 94% and 85%, respectively. By using this system, a GFP(uv)-beta1,3-N-acetylglucosaminyltransferase 2 (GFP(uv)-beta3GnT2) fusion protein is successfully expressed in silkworm larvae with less protein degradation and without larvae liquefaction
additional information
-
v-cath-deletion mutant, chloramphenicol acetyl transferase gene replaced the cathepsin gene
additional information
Cydia pomonella granulovirus CpGV-M1
-
v-cath-deletion mutant, chloramphenicol acetyl transferase gene replaced the cathepsin gene
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
biotechnology
-
this CPD-BmMNPV bacmid system provides rapid protein production in silkworms and can be used for the production of recombinant eukaryotic proteins without proteolytic degradation