Information on EC 3.4.22.50 - V-cath endopeptidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.22.50
-
RECOMMENDED NAME
GeneOntology No.
V-cath endopeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endopeptidase of broad specificity, hydrolyzing substrates of both cathepsin L and cathepsin B
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
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CAS REGISTRY NUMBER
COMMENTARY hide
316365-69-2
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9001-92-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
bat
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
CpGV-M1
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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1155227 A+, 1155228 A+, 1155230 A+, 1155232 A+, 1155237 A+, 1155239 A+, 1155251 A+, 1155252 A+, 1155254 A+, 1155256 A+, 1155258 A+, 1155260 A+, 1155262 A+, 1155275 A+, 1155276 A+, 1155277 A+, 1155282 A+, 1155284 A+, 1155290 A+, 1155300 A+, 1155301 A+, 1155305 A+, 1155308 A+, 1155312 A+, 1155314 A+, 1155317 A+, 1155322 A+, 1155323 A+, 1155324 A+, 1155327 A+, 1155328 A+, 1155353 A+, 1155357 A+, 1155358 A+, 1155364 A+, 1155369 A+, 1155377 A+, 1155378 A+, 1155383 A+, 1155390 A+, 1155391 A+, 1155395 A+, 1155412 A+, 1155419 A+, 1155433 A+, 1155436 A+, 1155439 A+, 1155451 A+, 1155455 A+, 1155468 A+, 1155471 A+, 1155485 A+, 1155489 A+, 1155493 A+, 1155494 A+, 1155499 A+, 1155505 A+, 1155507 A+, 1155508 A+, 1155513 A+, 1161896 A+, 1207436 A+, 1207471 A+, 1208239 A+, 1210156 A+, 1213583 A+, 1213693 A+, 1213710 A+, 1213718 A+, 1213733 A+, 1213746 A+, 1213752 A+, 1213759 A+, 1247189 A+, 1274936 A+, 1280540 A+, 1281147 A+, 1284867 A+, 1284875 A+, 1298850 A+, 1307581 A+, 1308260 A+, 1308345 A+, 1308458 A+, 1308562 A+, 1309054 A+, 1309863 A+, 1318548 A+, 1349793 A+, 137261 A+, 137263 A+, 137270 A+, 1406172 A+, 1409165 A+, 1419908 A+, 1419940 A+, 1419974 A+, 1420021 A+, 1420063 A+, 1420188 A+, 1423492 A+, 1423674 A+, 1423802 A+, 1423915 A+, 1443341 A+, 1521363 A+, 1611187 A+, 1643144 A+, 1670714 A+, 1813351 A+, 1916973 A+, 1919439 A+, 1978926 A+, 1978944 A+, 1978952 A+, 1978955 A+, 1978959 A+, 1978968 A+, 1978971 A+, 1978989 A+, 1978994 A+, 1979015 A+, 1979021 A+, 1979039 A+, 1979047 A+, 1979059 A+, 1979071 A+, 2107562 A+, 2107624 A+, 2107846 A+, 2108039 A+, 2114256 A+, 2116757 A+, 2116922 A+, 2116923 A+, 2116929 A+, 2116939 A+, 2116968 A+, 2117020 A+, 2117137 A+, 2117155 A+, 2117156 A+, 2132784 A+, 2147252 A+, 2148252 A+, 2148679 A+, 2148735 A+, 2148832 A+, 2154333 A+, 2154474 A+, 2154475 A+, 2154487 A+, 2154489 A+, 2154538 A+, 2154551 A+, 2154591 A+, 2154642 A+, 2154674 A+, 2154797 A+, 2155218 A+, 2155252 A+, 2155261 A+, 2155268 A+, 2155329 A+, 2155332 A+, 2155372 A+, 2155379 A+, 2155402 A+, 2155444 A+, 2155513 A+, 2176297 A+, 2203997 A+, 2209352 A+, 2343762 A+, 2343767 A+, 2343768 A+, 2343773 A+, 2343774 A+, 2343785 A+, 2343786 A+, 2343788 A+, 2343796 A+, 2343798 A+, 2343802 A+, 2343806 A+, 2343807 A+, 2343808 A+, 2343812 A+, 2343818 A+, 2343822 A+, 2343824 A+, 2343826 A+, 2343829 A+, 2343835 A+, 2343844 A+, 2343845 A+, 2343849 A+, 2343868 A+, 2343873 A+, 2343884 A+, 2380699 A+, 2381224 A+, 2381234 A+, 2413309 A+, 2413824 A+, 2419352 A+, 2422259 A+, 2422326 A+, 2423175 A+, 2427691 A+, 2429697 A+, 2435942 A+, 2440830 A+, 2445570 A+, 2450288 A+, 2450382 A+, 2450505 A+, 2450710 A+, 2518203 A+, 2525095 A+, 2525359 A+, 2532683 A+, 2533597 A+, 2547853 A+, 2547919 A+, 2548006 A+, 2548126 A+, 2548137 A+, 2548194 A+, 2548246 A+, 2548349 A+, 2548366 A+, 2548823 A+, 2549348 A+, 2550220 A+, 2550291 A+, 2550308 A+, 2569981 A+, 2672075 A+, 2681195 A+, 2728304 A+, 2772674 A+, 2931992 A+, 2937400 A+, 29385 A+, 3006934 A+, 3006950 A+, 3006953 A+, 3006967 A+, 3006970 A+, 3006973 A+, 3006978 A+, 3007011 A+, 3007022 A+, 3007028 A+, 3007030 A+, 3007032 A+, 3007034 A+, 30365 A+, 36505 A+, 36952 A+, 638872 A+, 647704 A+, 647941 A+, 647977 A+, 647985 A+, 652221 A+, 665912 A+, 665915 A+, 665954 A+, 678529 A+, 678912 A+, 679629 A+, 681195 A+, 683531 A+, 683662 A+, 691695 A+, 700877 A+, 701273 A+, 707045 A+, 707591 A+, 707615 A+, 710205 A+, 712089 A+, 713069 A+, 731168 A+, 731220 A+, 731340 A+, 732502 A+, 733635 A+, 741830 A+, 743558 A+, 744812 A+, 750133 A+, 750883 A+, 751759 A+, 752819 A+, 764781 A+, 764905 A+, 765168 A+, 765188 A+, 765196 A+, 765346 A+, 765356 A+, 765361 A+, 765392 A+, 765396 A+, 765405 A+, 765459 A+, 765522 A+, 765840 A+, 765859 A+, 765860 A+, 769530 A+, 769811 A+, 791773 A+, 821436 A+, 822394 A+, 822889 A+, 822895 A+, 822911 A+, 822922 A+, 822941 A+, 822966 A+, 822973 A+, 823021 A+, 823029 A+, 823032 A+, 823064 A+, 823069 A+, 823079 A+, 829412 A+, 829476 A+, 829479 A+, 829512 A+, 829521 A+, 829574 A+, 829652 A+, 829675 A+, 829684 A+, 829687 A+, 829697 A+, 829713 A+, 829729 A+, 829763 A+, 830123 A+, 830325 A+, 830340 A+, 830373 A+, 831232 A+, 831234 A+, 831295 A+, 831309 A+, 831645 A+, 831653 A+, 831752 A+, 831779 A+, 831836 A+, 831853 A+, 831886 A+, 831908 A+, 831997 A+, 846129 A+, 846177 A+, 863119 A+, 863120 A+, 863121 A+, 871376 A+, 886267 A+, 907476 A+, 908655 A+, 924104 A+, 924171 A+, 928765 A+, 938412 A+, 1155243 A+++, 1155247 A+++, 1155248 A+++, 1155253 A+++, 1155263 A+++, 1155264 A+++, 1155268 A+++, 1155304 A+++, 1155309 A+++, 1155311 A+++, 1155315 A+++, 1155332 A+++, 1155333 A+++, 1155340 A+++, 1155346 A+++, 1155350 A+++, 1155366 A+++, 1155442 A+++, 1155453 A+++, 1155459 A+++, 1155479 A+++, 1155486 A+++, 1155491 A+++, 1155509 A+++, 1155515 A+++, 36641 A+++, 744941 A+++, 764841 A+++, 764937 A+++, 821507 A+++, 829417 A+++, 829446 A+++, 853519 A+++, 856344 A+++, 863126 A+++, 925494 A+++
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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1155187 A+, 1155278 A+, 1155343 A+, 1155344 A+, 1155349 A+, 1155371 A+, 1155373 A+, 1155401 A+, 1155402 A+, 1155407 A+, 1155415 A+, 1155427 A+, 1155429 A+, 1155433 A+, 1155443 A+, 1155452 A+, 1155457 A+, 1155464 A+, 1155466 A+, 1155468 A+, 1155470 A+, 1155475 A+, 1155477 A+, 1155497 A+, 1155511 A+, 1213514 A+, 1213657 A+, 1213773 A+, 1253712 A+, 1258013 A+, 1259531 A+, 1280995 A+, 1296877 A+, 1307913 A+, 1308269 A+, 1320171 A+, 1399015 A+, 1399016 A+, 1406062 A+, 1406219 A+, 1406231 A+, 1420054 A+, 1420185 A+, 1420193 A+, 1420331 A+, 1423735 A+, 1478741 A+, 1588798 A+, 1978921 A+, 1978951 A+, 1978960 A+, 1978974 A+, 1978977 A+, 1978978 A+, 1978982 A+, 1979028 A+, 1979046 A+, 1979055 A+, 1979072 A+, 2106708 A+, 2106828 A+, 2106970 A+, 2115796 A+, 2116851 A+, 2116878 A+, 2116929 A+, 2116939 A+, 2116968 A+, 2116970 A+, 2117156 A+, 2117157 A+, 2117167 A+, 2147252 A+, 2148252 A+, 2154484 A+, 2154523 A+, 2154666 A+, 2155041 A+, 2155444 A+, 2192503 A+, 2226903 A+, 2263524 A+, 2343836 A+, 2343839 A+, 2344570 A+, 2348681 A+, 2377814 A+, 2381155 A+, 2381232 A+, 2381237 A+, 2381240 A+, 2416330 A+, 2423240 A+, 2425336 A+, 2426540 A+, 2444306 A+, 2450536 A+, 2499953 A+, 2503027 A+, 2533241 A+, 2548047 A+, 2548154 A+, 2548240 A+, 2548287 A+, 2550146 A+, 2550495 A+, 2683213 A+, 2691194 A+, 2692234 A+, 2692403 A+, 2708884 A+, 2850588 A+, 3006953 A+, 3006956 A+, 3006959 A+, 3006987 A+, 3006988 A+, 3007031 A+, 3007041 A+, 3007046 A+, 667486 A+, 667867 A+, 669514 A+, 669718 A+, 678529 A+, 679028 A+, 682861 A+, 683042 A+, 695956 A+, 697066 A+, 698663 A+, 699337 A+, 699648 A+, 700519 A+, 701409 A+, 708407 A+, 710807 A+, 712089 A+, 718306 A+, 740975 A+, 748065 A+, 748618 A+, 748955 A+, 750493 A+, 765026 A+, 765347 A+, 765370 A+, 765380 A+, 765392 A+, 765415 A+, 765420 A+, 765433 A+, 765854 A+, 766109 A+, 766121 A+, 769940 A+, 822219 A+, 822976 A+, 829621 A+, 831886 A+, 831940 A+, 909933 A+, 1136974 A+++, 1155347 A+++, 1155354 A+++, 1155379 A+++, 1155418 A+++, 1155421 A+++, 1155432 A+++, 1155467 A+++, 1155476 A+++, 1155482 A+++, 1155489 A+++, 664845 A+++, 778994 A+++, 831882 A+++
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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1005499 A+, 1104704 A+, 1155221 A+, 1155222 A+, 1155224 A+, 1155235 A+, 1155238 A+, 1155245 A+, 1155257 A+, 1155269 A+, 1155270 A+, 1155271 A+, 1155273 A+, 1155274 A+, 1155283 A+, 1155288 A+, 1155292 A+, 1155294 A+, 1155295 A+, 1155306 A+, 1155307 A+, 1155330 A+, 1155343 A+, 1155352 A+, 1155384 A+, 1155385 A+, 1155393 A+, 1155399 A+, 1155403 A+, 1155408 A+, 1155417 A+, 1155446 A+, 1155478 A+, 1155514 A+, 1209384 A+, 1213548 A+, 1223258 A+, 1256697 A+, 1256813 A+, 1256858 A+, 1257212 A+, 1257240 A+, 1258013 A+, 1259003 A+, 1268121 A+, 1309032 A+, 1406024 A+, 1407963 A+, 1419940 A+, 1423345 A+, 1423794 A+, 1423802 A+, 1423871 A+, 1428494 A+, 1443341 A+, 1455906 A+, 1481045 A+, 1491868 A+, 1565336 A+, 1595323 A+, 1596422 A+, 1826512 A+, 1978923 A+, 1978929 A+, 1978934 A+, 1978936 A+, 1978938 A+, 1978944 A+, 1978950 A+, 1978956 A+, 1979092 A+, 2107679 A+, 2148712 A+, 2148744 A+, 2154381 A+, 2154403 A+, 2154431 A+, 2154515 A+, 2154666 A+, 2155194 A+, 2155210 A+, 2155384 A+, 2343733 A+, 2343771 A+, 2343778 A+, 2343782 A+, 2343783 A+, 2343791 A+, 2343796 A+, 2343807 A+, 2343847 A+, 2343853 A+, 2421470 A+, 2424225 A+, 2428299 A+, 2450510 A+, 2458130 A+, 2458138 A+, 2497838 A+, 2533058 A+, 2547856 A+, 2547943 A+, 2550061 A+, 2550096 A+, 2566417 A+, 2646273 A+, 2772674 A+, 2867876 A+, 29385 A+, 29386 A+, 3006955 A+, 3006956 A+, 3007001 A+, 3007035 A+, 30139 A+, 36650 A+, 36663 A+, 36948 A+, 669364 A+, 695979 A+, 740429 A+, 741304 A+, 741858 A+, 743919 A+, 745892 A+, 760365 A+, 764888 A+, 764953 A+, 765096 A+, 765334 A+, 765343 A+, 765370 A+, 765522 A+, 765569 A+, 766082 A+, 770762 A+, 820283 A+, 820308 A+, 821386 A+, 821436 A+, 823021 A+, 823969 A+, 829440 A+, 829554 A+, 829570 A+, 829701 A+, 830391 A+, 831593 A+, 831688 A+, 831726 A+, 831779 A+, 853188 A+, 854872 A+, 862945 A+, 866086 A+, 876254 A+, 877289 A+, 905688 A+, 929234 A+, 942491 A+, 1155240 A+++, 1155299 A+++, 1155302 A+++, 1155313 A+++, 1155319 A+++, 1155341 A+++, 1155355 A+++, 1155409 A+++, 34488 A+++, 638840 A+++, 785001 A+++, 823734 A+++, 831783 A+++, 924358 A+++
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
shrimp
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Automatic Mining of ENzyme DAta
snake
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
toad
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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the absence of v-cathepsin results in poor melanization and liquefaction of Cydia pomonella larvae
physiological function
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v-cathepsin is involved in the degradation of larval tissues of Cydia pomonella
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
azocasein + H2O
?
show the reaction diagram
Azocoll + H2O
?
show the reaction diagram
N-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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-
-
?
N-benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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-
-
?
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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-
-
?
N-benzyloxycarbonyl-Val-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
N-benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
antipain
0.00005 mM, complete inhibition
chicken cystatin
0.0001 mM, complete inhibition
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iodoacetate
0.05 mM, 80% inhibition
leupeptin
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
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10 microM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Autographa californica multiple nucleopolyhedrovirus chitinase
-
a proposed folding chaperone for the progenitor of V-CATH
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003
Z-Arg-Arg-7-amido-4-methylcoumarin
-
0.022
Z-Leu-Arg 7-amido-4-methylcoumarin
-
0.039
Z-Phe-Arg 7-amido-4-methylcoumarin
-
0.043
Z-Val-Arg-7-amido-4-methylcoumarin
-
0.058
Z-Val-Val-Arg 7-amido-4-methylcoumarin
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.73
Z-Arg-Arg-7-amido-4-methylcoumarin
0.95
Z-Phe-Arg 7-amido-4-methylcoumarin
Autographa californica nucleopolyhedrovirus
P25783
-
0.94
Z-Val-Arg-7-amido-4-methylcoumarin
Autographa californica nucleopolyhedrovirus
P25783
-
0.51
Z-Val-Val-Arg 7-amido-4-methylcoumarin
Autographa californica nucleopolyhedrovirus
P25783
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 5.5
bell-shaped profile, hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin and Z-Arg-Arg-7-amino-4-methylcoumarin
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 7.5
hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin
4 - 7.5
hydrolysis of Z-Arg-Arg-7-amino-4-methylcoumarin
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
N-glycosylation may occur at Asn158
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D
expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
the introduction of the v-cath gene into the genome of Anticarsia gemmatalis multiple nucleopolyhedrovirus improves its insecticidal activity against Anticarsia gemmatalis larvae
biotechnology
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this CPD-BmMNPV bacmid system provides rapid protein production in silkworms and can be used for the production of recombinant eukaryotic proteins without proteolytic degradation