Information on EC 3.4.22.43 - cathepsin V

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The expected taxonomic range for this enzyme is: Coelomata

EC NUMBER
COMMENTARY hide
3.4.22.43
-
RECOMMENDED NAME
GeneOntology No.
cathepsin V
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
The recombinant enzyme hydrolyses proteins (serum albumin, collagen) and synthetic substrates (Z-Phe-Arg-NHMec > Z-Leu-Arg-NHMec > Z-Val-Arg-NHMec)
show the reaction diagram
Cathepsin V is a human lysosomal cysteine endopeptidase of family C1, papain family that is maximally active at pH 5.7 and unstable at neutral pH. Compound E-64, leupeptin and chicken cystatin are inhibitors. Human cathepsin V shows expression restricted to thymus, testis, corneal epithelium and some colon and breast carcinomas. Human gene locus: 9q22.2; endopeptidase
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
223670-00-6
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
RNAi-mediated depletion of the enzyme effectively abrogates ectopic E2F1-induced apoptosis, coupled with reduced lysosomal membrane permeabilization and mitochondrial membrane depolarization. Enzyme knockdown also inhibits apoptosis mediated by the endogenous E2F1 activated by DNA damage. Enzyme depletion in cancer cells results in inhibition of histone deacetylase inhibitor-induced apoptosis
metabolism
-
cathepsin V participates in the production of enkephalin and neuropeptide Y neurotransmitters in secretory vesicles
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-ALRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
best substrate
-
-
?
2-aminobenzoyl-EE-epsilon-amino-caproic acid-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KK-epsilon-amino-caproic acid-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLRSIKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-KLR + SIKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
best substrate
-
-
?
2-aminobenzoyl-KLRSXKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-KLR + SXKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
X is varied with diverse amino acids, highest activity with Ile, kinetics, detailed overview
-
-
?
2-aminobenzoyl-KLRXSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-KLR + XSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
X is varied with diverse amino acids, highest activity with Val, kinetics, detailed overview
-
-
?
2-aminobenzoyl-KLXSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-KLX + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
X is varied with diverse amino acids, highest activity with Arg, kinetics, detailed overview
-
-
?
2-aminobenzoyl-KPPVVLLPDQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
2-aminobenzoyl-KPPVV + LLPDQ-N-[2,4-dinitrophenyl]ethylenediamine
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KPVSTEQLAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
2-aminobenzoyl-KPVS + TEQLAQ-N-[2,4-dinitrophenyl]ethylenediamine
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KXRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
X is varied with diverse amino acids, highest activity with Leu, kinetics, detailed overview
-
-
?
2-aminobenzoyl-LFEKQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
2-aminobenzoyl-LF + EKQ-N-[2,4-dinitrophenyl]ethylenediamine
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-VLFEKKQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
2-aminobenzoyl-VLF + EKKQ-N-[2,4-dinitrophenyl]ethylenediamine
show the reaction diagram
-
selective substrate for cathepsin V when compared with cathepsins B, L, and S
-
-
?
2-aminobenzoyl-VLFEKKVYLQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
2-aminobenzoyl-VLF + EKKVY + LQ-N-[2,4-dinitrophenyl]ethylenediamine
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-VLFEKQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
2-aminobenzoyl-VLF + EKQ-N-[2,4-dinitrophenyl]ethylenediamine
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-XLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
X is varied with diverse amino acids, highest activity with Ala, kinetics, detailed overview
-
-
?
Albumin + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Boc-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Bovine serum albumin + H2O
?
show the reaction diagram
-
-
-
-
?
Cbz-Phe-Arg-7-amido-4-methylcoumarin + H2O
Cbz-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Elastin + H2O
?
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
kininogen fragments + H2O
?
show the reaction diagram
-
kininogenase activity with high and low molecular weight kininogens
Lys-bradykinin is the major product
-
?
MHC class II-associated invariant chain + H2O
?
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-L-Arg-4-methylcoumarin 7-amide + H2O
N-benzyloxycarbonyl-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
very low activity
-
-
N-benzyloxycarbonyl-L-Arg-L-Arg-4-methylcoumarin 7-amide + H2O
N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
very low activity
-
-
N-benzyloxycarbonyl-L-Phe-L-Arg-4-methylcoumarin 7-amide + H2O
N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
neuropeptide Y + H2O
?
show the reaction diagram
-
-
-
-
?
plasminogen + H2O
?
show the reaction diagram
proenkephalin + H2O
(Met)enkephalin + ?
show the reaction diagram
-
-
-
-
?
Type I collagen + H2O
?
show the reaction diagram
-
cleavage of telopeptide region
-
-
?
Z-Arg-Arg-4-methoxy-2-nitroanilide + H2O
Z-Arg-Arg + 4-methoxy-2-nitroaniline
show the reaction diagram
-
-
-
-
?
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
Z-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Z-Leu-Arg-7-amido-4-methylcoumarin + H2O
Z-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Z-Phe-Arg-4-methylcoumarin-7-amide + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Elastin + H2O
?
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
neuropeptide Y + H2O
?
show the reaction diagram
-
-
-
-
?
plasminogen + H2O
?
show the reaction diagram
proenkephalin + H2O
(Met)enkephalin + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
-
stimulates at 0.3 M
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R)-1-{2-[butyl(dichloro)-l4-tellanyl]phenyl}ethyl methyl ether
-
less than 10% residual activity at 0.001 mM
(1R)-1-{2-[dibromo(butyl)-l4-tellanyl]phenyl}ethyl methyl ether
-
less than 10% residual activity at 0.001 mM
(1S)-1-{2-[butyl(dichloro)-l4-tellanyl]phenyl}ethyl methyl ether
-
less than 10% residual activity at 0.001 mM
(1S)-1-{2-[dibromo(butyl)-l4-tellanyl]phenyl}ethyl methyl ether
-
less than 10% residual activity at 0.001 mM
1,3,5-trihydroxy-2,8-bis(3-methylbut-2-enyl)-10-methyl-9-acridone
-
-
1,3,5-trihydroxy-4-methoxy-10-methyl-2,8-bis(3-methylbut-2-enyl)acridin-9(10H)-one
-
-
2,3-dihydro-4,9-dihydroxy-2-(2-hydroxy-propan-2-yl)-11-methoxy-10-methylfuro[3,2-b]acridin-5(10H)-one
-
-
2-[butyl(dichloro)-l4-tellanyl]benzyl methyl ether
-
about 10% residual activity at 0.001 mM
2-[dibromo(butyl)-l4-tellanyl]benzyl methyl ether
-
less than 10% residual activity at 0.001 mM
3,4-dihydro-3,5,8-trihydroxy-6-methoxy-2,2,7-trimethyl-2H-pyrano[2,3-a]acridin-12(7H)-one
-
-
4-methylpiperazine-1-carboxylic acid [1-[(3-benzenesulfonyl-1-phenethylallyl)carbamoyl]-2-phenylethyl]amide
-
i.e. APC-3316
-
4-morpholincarbonyl-Leu-homophenylalaninevinyl sulfone-phenyl
-
-
-
5-hydroxynoracronycine
-
-
Calpeptin
-
-
cathepsin V propeptide
-
CatV PP, tight-binding inhibitor
-
chondroitin 4-sulfate
-
specific inhibition of elastolytic activity, formation of specific complexes with the enzyme, no inhibition of the activity with Z-Phe-Arg-4-methyl-7-coumaryl amide
chymostatin
-
-
citibrasine
-
-
citrusinine-I
-
-
citrusinine-II
-
-
clitocypin
-
-
-
Cystatin
cystatin C
-
endogenous inhibitor of cathepsin V analyzed, expression of cystatin C increased in stenotic valves, cystatin C protein particularly found in areas with infiltrates of inflammatory cells
-
cystatin M/E
cystatins
-
-
-
fragment p41 of major histocompatibility complex class II-associated invariant chain
-
inhibitory to human cathepsin V, cathepsin L, cathepsin K, cathepsin F with Ki values in the low nanomolar range. Ki values are sufficiently low to ensure complex formation at physiological concentrations. Regulation of the proteolytic activity of most of the cysteine cathepsins by the p41 fragment is an important and widespread control mechanism of antigen presentation
-
glycocitrine-I
-
-
glycocitrine-IV
-
-
iodoacetic acid
-
-
macrocypin-1
-
-
-
morpholineurea-leucyl-homophenylalanine-vinylsulfone phenyl
-
-
morpholineurea-naphthyl-homophenylalanine-vinylsulfone naphthyl
-
-
-
peptidl vinyl sulfones
-
-
-
pyranofoline
-
-
serpin
-
cofactors fine tuning serpin activity in the extracellular milieu analyzed, DNA accelerate the rate at which the model serpin MENT inhibit cathepsin V up to 50-fold, primarily effected via the protease and secondarily by the recruitment of the DNA as a template onto which cathepsin V and MENT are bound, altered substrate turnover and conformational change within the protease observed, enzyme concentration constant at 0.1 nM, serpin concentration varying between 0.5 and 60 nM with a maximum of 5 nM serpin in the presence of DNA
-
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
VBY-825
-
reversible cathepsin inhibitor with high potency against cathepsins V
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
the recombinant proenzyme is autocatalytically activated in vitro by incubation at pH 4.0 and 30C
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0016
2-aminobenzoyl-ALRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37C
0.0017
2-aminobenzoyl-EE-epsilon-amino-caproic acid-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37C
0.002
2-aminobenzoyl-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37C
0.0008
2-aminobenzoyl-KK-epsilon-amino-caproic acid-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37C
0.0006
2-aminobenzoyl-KLRSIKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37C
0.0012
2-aminobenzoyl-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37C
0.0006
2-aminobenzoyl-KLRVSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37C
0.0044
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25C
0.0024
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin
-
in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25C
0.0041 - 0.0048
Z-Phe-Arg-4-methyl-7-coumaryl amide
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4
2-aminobenzoyl-ALRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Homo sapiens
-
recombinant enzyme, pH 5.5, 37C
1.3
2-aminobenzoyl-EE-epsilon-amino-caproic acid-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine
Homo sapiens
-
recombinant enzyme, pH 5.5, 37C
0.6
2-aminobenzoyl-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine
Homo sapiens
-
recombinant enzyme, pH 5.5, 37C
1.7
2-aminobenzoyl-KK-epsilon-amino-caproic acid-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine
Homo sapiens
-
recombinant enzyme, pH 5.5, 37C
1.5
2-aminobenzoyl-KLRSIKQ-N-(2,4-dinitrophenyl)ethylenediamine
Homo sapiens
-
recombinant enzyme, pH 5.5, 37C
1.3
2-aminobenzoyl-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Homo sapiens
-
recombinant enzyme, pH 5.5, 37C
0.9
2-aminobenzoyl-KLRVSKQ-N-(2,4-dinitrophenyl)ethylenediamine
Homo sapiens
-
recombinant enzyme, pH 5.5, 37C
156
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Homo sapiens
-
in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25C
19
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin
Homo sapiens
-
in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25C
3.4 - 3.7
Z-Phe-Arg-4-methyl-7-coumaryl amide
additional information
additional information
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
496
2-aminobenzoyl-KPPVVLLPDQ-N-[2,4-dinitrophenyl]ethylenediamine
Homo sapiens
-
at 37C, in 0.1 M sodium acetate buffer, pH 5.5
160418
903
2-aminobenzoyl-KPVSTEQLAQ-N-[2,4-dinitrophenyl]ethylenediamine
Homo sapiens
-
at 37C, in 0.1 M sodium acetate buffer, pH 5.5
160417
4528
2-aminobenzoyl-LFEKQ-N-[2,4-dinitrophenyl]ethylenediamine
Homo sapiens
-
at 37C, in 0.1 M sodium acetate buffer, pH 5.5
160419
3055
2-aminobenzoyl-VLFEKKQ-N-[2,4-dinitrophenyl]ethylenediamine
Homo sapiens
-
at 37C, in 0.1 M sodium acetate buffer, pH 5.5
160421
6833
2-aminobenzoyl-VLFEKKVYLQ-N-[2,4-dinitrophenyl]ethylenediamine
Homo sapiens
-
at 37C, pH 5.5
160416
4277
2-aminobenzoyl-VLFEKQ-N-[2,4-dinitrophenyl]ethylenediamine
Homo sapiens
-
at 37C, in 0.1 M sodium acetate buffer, pH 5.5
160420
35000
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Homo sapiens
-
in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25C
1213
7900
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin
Homo sapiens
-
in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25C
2442
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0012
1,3,5-trihydroxy-2,8-bis(3-methylbut-2-enyl)-10-methyl-9-acridone
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.0005
1,3,5-trihydroxy-4-methoxy-10-methyl-2,8-bis(3-methylbut-2-enyl)acridin-9(10H)-one
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.0044
2,3-dihydro-4,9-dihydroxy-2-(2-hydroxy-propan-2-yl)-11-methoxy-10-methylfuro[3,2-b]acridin-5(10H)-one
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.0017
3,4-dihydro-3,5,8-trihydroxy-6-methoxy-2,2,7-trimethyl-2H-pyrano[2,3-a]acridin-12(7H)-one
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.0102
cathepsin V propeptide
-
varying concentrations tested
-
0.0002
citibrasine
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.001
citrusinine-I
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.0042
citrusinine-II
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.00000008
clitocypin
-
pH and temperature not specified in the publication
-
0.00000047
cystatin M/E
-
pH and temperature not specified in the publication
-
0.0000000072
fragment p41 of major histocompatibility complex class II-associated invariant chain
-
pH 6.0, 25C
-
0.01
glycocitrine-I
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.0011
glycocitrine-IV
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.00000069
macrocypin-1
-
pH and temperature not specified in the publication
-
0.00000034
mutant N64A cystatin M/E
-
pH 5.5, 22C
-
0.00000025
VBY-825
-
apparent value, in 50 mM MES pH 6.5, 2.5 mM DTT, 2.5 mM EDTA, 100 mM NaCl, 0.01% (w/v) bovine serum albumin, and 10% (v/v) DMSO, at 25C
0.00000047
wild-type cystatin M/E
-
pH 5.5, 22C
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0039
1,3,5-trihydroxy-2,8-bis(3-methylbut-2-enyl)-10-methyl-9-acridone
Homo sapiens
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.0025
1,3,5-trihydroxy-4-methoxy-10-methyl-2,8-bis(3-methylbut-2-enyl)acridin-9(10H)-one
Homo sapiens
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.0052
2,3-dihydro-4,9-dihydroxy-2-(2-hydroxy-propan-2-yl)-11-methoxy-10-methylfuro[3,2-b]acridin-5(10H)-one
Homo sapiens
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.0028
3,4-dihydro-3,5,8-trihydroxy-6-methoxy-2,2,7-trimethyl-2H-pyrano[2,3-a]acridin-12(7H)-one
Homo sapiens
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.048
5-hydroxynoracronycine
Homo sapiens
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.0012
citibrasine
Homo sapiens
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.0022
citrusinine-I
Homo sapiens
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.01
citrusinine-II
Homo sapiens
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.025
glycocitrine-I
Homo sapiens
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.0022
glycocitrine-IV
Homo sapiens
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
0.044
pyranofoline
Homo sapiens
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6
-
-
5.5 - 6.5
-
assay at
6.5
-
plasminogen digestion assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 6
-
cathepsin V is active at pH 4.0 and 6.0
4 - 7.2
-
less than 50% of maximal activity above and below
additional information
-
bell-shaped pH profile
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at room temperature
25
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
expression in aorta valve, control valves and stenotic valves analyzed
Manually annotated by BRENDA team
-
cathepsin V localized to endothelial cells in areas rich of neovascularization
Manually annotated by BRENDA team
-
ZR-75-1, Hs-578T, MDA-MB435
Manually annotated by BRENDA team
-
recombinant human cathepsin V for inhibition studies, N-terminal propeptide domains as inhibitors of cathepsin V
Manually annotated by BRENDA team
-
thymic and corneal
Manually annotated by BRENDA team
-
in the human thymic cortex, CTSV is the predominately expressed protease
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
the enzyme is secreted
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24000
-
mature protein, SDS-PAGE
35500
-
proprotein, SDS-PAGE
37000
-
preproprotein, SDS-PAGE
37330
-
preproprotein, containing a 17 amino acid signal peptide, a 96 amino acid propeptide and a 221 amino acid mature region, calculation from sequence of cDNA
39000
-
glycosylated enzyme form, SDS-PAGE
41000
-
glycosylated enzyme form, SDS-PAGE
52000
-
recombinant GST-tagged enzyme
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 23999, amino acid sequence calculation of the mature enzyme, 2 * 31000, recombinant mature enzyme from insect cells and Pichia pastoris, 2 * 38000-45000, recombinant proenzyme from Pichi pastoris
additional information
-
three-dimensional two-domain structure analysis at 1.6 A resolution, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
proteolytic modification
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cathepsin V in complex with clitocypin, vapor diffusion method, using 0.4 M Li2SO4, 12% (w/v) polyethylene glycol 800, 20% (v/v) glycerol
-
molecular modeling of complex with fragment p41 of major histocompatibility complex class II-associated invariant chain
-
space group P6422, crystal structure at 1.6 A resolution
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 5.5
-
very stable
668763
6.5
-
2 h, residual acitviy
137269
7
-
1 h, residual acitviy
137269
additional information
-
rapid loss of activity at neutral pH
668763
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
pH 5.5, loss of 60% activity within 20 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gel filtration, pre-activated before use
-
gel filtration, recombinant human cathepsin V
-
Ni-NTA column chromatography
-
recombinant enzyme from Pichia pastoris to homogeneity, activated mature native enzyme by hyrophobic interaction chromatography, recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography
-
recombinant enzyme from Pichia pastoris, further purified by gel filtration after activation by pepsin treatment
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cathepsin V with mutated glycosylation site is expressed in Pichia pastoris
-
expressed in Escherichia coli
-
expressed in Pichia pastoris
-
expressed in thymus of transgenic Mus musculus
-
expression in Escherichia coli
-
expression in Pichia pastoris
-
from cDNA libraries, the gene maps to chromosome 9q21, DNA and amino acid sequence determination and analysis, genomic organization, expression in insect cells using the baculovirus system, in Pichia pastoris, and as GST-tagged enzyme with low activity in Escherichia coli
-
human cathepsins V expressed in Pichia pastoris
-
recombinant human cathepsin V produced using the Pichia pastoris expression system
-
recombinant procathepsin V is expressed in soluble form in the cytoplasm of Escherichia coli Rosetta-gami B (DE3) pLysS cells
-
transgenic expression in cathepsin L-deficient mice, phylogenetic tree
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
CTSL2 transcript levels are strongly elevated in endometrial cancer, particularly in G3 tumors
-
E2F1 activates cathepsin like 2 gene promoter activity
-
there is decreased expression of cathepsin V in lesional atopic dermatitis and psoriasis epidermis at the mRNA level as well as the protein level
-
tissue necrosis factor alpha increases cathepsin V expression and activity by 2fold. Tissue necrosis factor alpha and co-culturing with THP-1 monocytes stimulates a 460% increase in cathepsin V active enzyme
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
the enzyme can complement cathepsin L-deficient mice by expression of cathepsin V in epidermis and hair follicles, the mutant mice suffer from a complex skin phenotype consisting of hair loss and epidermal hyperplasia with hyperproliferation of basal epidermal keratinocytes, acanthosis, and hyperkeratosis, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
-
the enzyme is a potential marker for certain colon tumors and breast cancer
medicine
-
studies on the role of cathepsin V and cystatin C in the pathophysiology aortic stenosis