Information on EC 3.4.22.39 - adenain

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The expected taxonomic range for this enzyme is: unidentified adenovirus

EC NUMBER
COMMENTARY hide
3.4.22.39
-
RECOMMENDED NAME
GeneOntology No.
adenain
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-/-Xaa- and -Yaa-Xaa-Gly-Xaa-/-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid)
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
37353-41-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
human adenovirus
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Ile-Arg-Gly-Gly-NH)2-rhodamine
?
show the reaction diagram
-
-
-
-
?
(Leu-Arg-Gly-Gly)2-rhodamine + H2O
?
show the reaction diagram
-
-
-
-
?
(Leu-Arg-Gly-Gly-NH)2-rhodamine + H2O
?
show the reaction diagram
(N-carbobenzyloxy-Met-Arg-Gly-Gly-NH)2-rhodamine
?
show the reaction diagram
-
-
-
-
?
(N-carbobenzyloxy-Nle-Arg-Gly-Gly-NH)2-rhodamine
?
show the reaction diagram
-
-
-
-
?
(Nle-Arg-Gly-Gly-NH)2-rhodamine
?
show the reaction diagram
-
-
-
-
?
actin
36 kD protein
show the reaction diagram
-
-
-
?
core polypeptide pVI + H2O
?
show the reaction diagram
core polypeptide pVI + H2O
pVIc + ?
show the reaction diagram
human adenovirus
-
the enzyme cleaves DNA-bound pVI twice, once at its N terminus and then at its C terminus. Cleavage at the C terminus liberates pVIc, the 11-amino acid peptide cofactor
-
-
?
core polypeptide PVII
polypeptide VII
show the reaction diagram
epsilon-N-5-carboxytetramethylrhodamine-Arg lucifer yellow CH hydrazone
?
show the reaction diagram
-
-
-
-
?
Fibrin + H2O
?
show the reaction diagram
-
-
-
-
?
Leu-Ser-Gly-Gly-Ala-Phe-Ser-Trp
Leu-Ser-Gly-Gly + Ala-Phe-Ser-Trp
show the reaction diagram
-
-
-
?
Ly-AnLRGA-GFSWK-ctmr-R
?
show the reaction diagram
-
-
-
-
?
Ly-AnLRGG-AFSWK-ctmr-R
?
show the reaction diagram
-
-
-
-
?
Met-Glu-Gly-Gly-Ala-Lys-Lys
?
show the reaction diagram
-
-
-
-
?
Met-Phe-Gly-Gly-Ala-Lys-Lys-Arg
?
show the reaction diagram
-
-
-
-
?
Met-Ser-Gly-Gly-Ala-Phe-Ser-Trp
?
show the reaction diagram
-
-
-
-
?
N-acetyl-WLRGGARC(PT14)-NH2 + H2O
N-acetyl-WLRGG + ARC(PT14)-NH2
show the reaction diagram
human adenovirus
-
-
-
-
?
ovalbumin
37 kD protein
show the reaction diagram
-
-
-
?
preterminal proteins
?
show the reaction diagram
-
pVI, pVII, pVIII, IIIa, 11K
-
-
-
pVI
protein VI
show the reaction diagram
-
-
-
?
rhodamine 1,10-bis-(L-leucyl-L-arginylglycylglycine amide) tetrahydrochloride
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
core polypeptide pVI + H2O
?
show the reaction diagram
core polypeptide pVI + H2O
pVIc + ?
show the reaction diagram
human adenovirus
-
the enzyme cleaves DNA-bound pVI twice, once at its N terminus and then at its C terminus. Cleavage at the C terminus liberates pVIc, the 11-amino acid peptide cofactor
-
-
?
preterminal proteins
?
show the reaction diagram
-
pVI, pVII, pVIII, IIIa, 11K
-
-
-
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S)-2-(3,5-dichlorophenyl)-N-[(3S)-1-[2-[(2-[[(2E)-3-(methylsulfonyl)prop-2-en-1-yl]amino]-2-oxoethyl)amino]-2-oxoethyl]-2-oxopyrrolidin-3-yl]butanamide
human adenovirus
-
-
2,2'-(hydroxynitrosohydrazono)bis-ethaneamine
-
NO generated from 2,2'-(hydroxynitrosohydrazono)bis-ethaneamine inhibits
3-[2-(3,5-dichlorophenyl)-2-methylpropanoyl]-4-methoxy-N-(2-[[(2E)-3-(methylsulfonyl)prop-2-en-1-yl]amino]-2-oxoethyl)benzamide
human adenovirus
-
-
4,4-dithiodipyridine
-
-
bestatin, L-transepoxy-succinyl-leucylamido-(4-guanido)-butane
-
-
-
bestatin, L-transepoxy-succinyl-leucylamido-(4-guanido)-butane tyrosine
-
-
-
glutamic acid
-
inhibition by displacement of viral DNA from complexes with enzyme
N-(2-[[(2-cyanopyrimidin-4-yl)methyl]amino]-2-oxoethyl)-3-[2-(3,5-dichlorophenyl)-2-methylpropanoyl]-4-methoxybenzamide
human adenovirus
-
-
N-(2-[[(2-cyanopyrimidin-4-yl)methyl]amino]-2-oxoethyl)-3-[2-(3,5-dichlorophenyl)-2-methylpropanoyl]-4-[(dimethylamino)oxy]benzamide
human adenovirus
-
-
N-[(2S)-2-(3,5-dichlorophenyl)-2-(ethylamino)acetyl]-3-methyl-L-valylglycyl-N-[(2E)-3-(methylsulfonyl)prop-2-en-1-yl]glycinamide
human adenovirus
-
-
N-[(2S)-2-(3-chlorophenyl)-2-[(methylsulfonyl)amino]acetyl]-L-phenylalanyl-N-(cyanomethyl)glycinamide
human adenovirus
-
-
N-[(3,5-dichlorophenyl)acetyl]-3-methyl-L-valylglycyl-N-[(2E)-3-(methylsulfonyl)prop-2-en-1-yl]glycinamide
human adenovirus
-
-
N-[2-[(cyanomethyl)amino]-2-oxoethyl]-3-[(3,5-dichlorophenyl)acetyl]-4-methoxybenzamide
human adenovirus
-
-
N-[2-[(cyanomethyl)amino]-2-oxoethyl]-3-[2-(3,5-dichlorophenyl)-2-methylpropanoyl]-4-methoxybenzamide
human adenovirus
-
-
N-[2-[(cyanomethyl)amino]-2-oxoethyl]-3-[2-(3,5-dichlorophenyl)-2-methylpropanoyl]-4-[(dimethylamino)oxy]benzamide
human adenovirus
-
-
N-[2-[(cyanomethyl)amino]-2-oxoethyl]-3-[2-(3,5-dichlorophenyl)propanoyl]-4-methoxybenzamide
human adenovirus
-
-
N-[4-[1-(3-aminopropyl)-2-hydroxy-2-nitrosohydrazino]butyl]-1,3-propanediamine
-
NO generated from N-[4-[1-(3-aminopropyl)-2-hydroxy-2-nitrosohydrazino]butyl]-1,3-propanediamine inhibits the enzyme
NaCl
-
inhibition by displacement of viral DNA from complexes with enzyme
NO
-
inhibits adenovirus replication by targeting the adenovirus proteinase, inhibition is reversible with dithiothreitol
p-chloromercuribenzoate
-
-
Phenylmethylsulfonylfluoride
-
-
Soybean trypsin inhibitor
-
-
-
tosyl-lysinechloromethyl ketone
-
-
tosylamide phenylethylchloromethyl ketone
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cysteine
-
activation
EDTA
-
stimulation
GVQSLKRRRCF
peptife VIc
human adenovirus
-
an 11-amino acid peptide (GVQSLKRRRCF) originating from the C terminus of the precursor protein pVI
-
polyE
-
interaction of enzyme-virion precursor protein pVI with polymers of glutamic acid leads to increase in substrate hydrolysis
-
viral DNA
virion precursor protein pVI
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00517
(Ile-Arg-Gly-Gly-NH)2-rhodamine
-
-
-
0.0034 - 0.0948
(Leu-Arg-Gly-Gly-NH)2-rhodamine
0.00474
(N-carbobenzyloxy-Met-Arg-Gly-Gly-NH)2-rhodamine
-
-
-
0.000758
(N-carbobenzyloxy-Nle-Arg-Gly-Gly-NH)2-rhodamine
-
-
-
0.0024
(Nle-Arg-Gly-Gly-NH)2-rhodamine
-
-
-
0.0014 - 0.005
rhodamine 110,bis-(L-leucyl-L-arginylglycylglycine amide),tetrahydrochloride
additional information
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide
human adenovirus
-
influence of mutant pVIcs on Km-value with (Leu-Arg-Gly-Gly-NH)2-rhodamine as substrate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0023 - 6
(Leu-Arg-Gly-Gly-NH)2-rhodamine
additional information
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide
human adenovirus
-
influence of mutant pVIcs on turnover number with (Leu-Arg-Gly-Gly-NH)2-rhodamine as substrate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00023
glutamic acid
-
37C, pH 8.0
0.000329
NaCl
-
37C, pH 8.0
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000002 - 0.000004
(2S)-2-(3,5-dichlorophenyl)-N-[(3S)-1-[2-[(2-[[(2E)-3-(methylsulfonyl)prop-2-en-1-yl]amino]-2-oxoethyl)amino]-2-oxoethyl]-2-oxopyrrolidin-3-yl]butanamide
0.000001
3-[2-(3,5-dichlorophenyl)-2-methylpropanoyl]-4-methoxy-N-(2-[[(2E)-3-(methylsulfonyl)prop-2-en-1-yl]amino]-2-oxoethyl)benzamide
human adenovirus
-
isoform AVP5, pH and temperature not specified in the publication; isoform AVP8, pH and temperature not specified in the publication
0.0000002 - 0.0000006
N-(2-[[(2-cyanopyrimidin-4-yl)methyl]amino]-2-oxoethyl)-3-[2-(3,5-dichlorophenyl)-2-methylpropanoyl]-4-methoxybenzamide
0.0000001
N-(2-[[(2-cyanopyrimidin-4-yl)methyl]amino]-2-oxoethyl)-3-[2-(3,5-dichlorophenyl)-2-methylpropanoyl]-4-[(dimethylamino)oxy]benzamide
human adenovirus
-
isoform AVP5, pH and temperature not specified in the publication; isoform AVP8, pH and temperature not specified in the publication
0.000001
N-[(2S)-2-(3,5-dichlorophenyl)-2-(ethylamino)acetyl]-3-methyl-L-valylglycyl-N-[(2E)-3-(methylsulfonyl)prop-2-en-1-yl]glycinamide
human adenovirus
-
IC50 below 0.000001 mM, isoform AVP8, pH and temperature not specified in the publication
0.00001
N-[(3,5-dichlorophenyl)acetyl]-3-methyl-L-valylglycyl-N-[(2E)-3-(methylsulfonyl)prop-2-en-1-yl]glycinamide
human adenovirus
-
isoform AVP5, pH and temperature not specified in the publication; isoform AVP8, pH and temperature not specified in the publication
0.0011 - 0.00274
N-[2-[(cyanomethyl)amino]-2-oxoethyl]-3-[(3,5-dichlorophenyl)acetyl]-4-methoxybenzamide
0.00003 - 0.00004
N-[2-[(cyanomethyl)amino]-2-oxoethyl]-3-[2-(3,5-dichlorophenyl)-2-methylpropanoyl]-4-methoxybenzamide
0.000007 - 0.00001
N-[2-[(cyanomethyl)amino]-2-oxoethyl]-3-[2-(3,5-dichlorophenyl)-2-methylpropanoyl]-4-[(dimethylamino)oxy]benzamide
0.00031 - 0.00095
N-[2-[(cyanomethyl)amino]-2-oxoethyl]-3-[2-(3,5-dichlorophenyl)propanoyl]-4-methoxybenzamide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
HeLa cell infected with adenovirus type 2
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Human adenovirus C serotype 2
Human adenovirus C serotype 2
Human adenovirus C serotype 2
Human adenovirus C serotype 2
Human adenovirus C serotype 2
Human adenovirus C serotype 2
Human adenovirus D serotype 8
Human adenovirus D serotype 8
Human adenovirus D serotype 8
Human adenovirus D serotype 8
Human adenovirus D serotype 8
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
gel filtration, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in complex with pVIc, microseeding vapor diffusion method
human adenovirus
structure at 1.6-A resolution of the human adenovirus proteinase in a covalent complex with its 11-amino-acid peptide cofactor
human adenovirus
-
hanging-drop vapour-diffusion technique
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Sf9/baculovirus cells
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
human adenovirus
-
expression in Escherichia coli
-
expression in Sf9/baculovirus cells
-
glutathione S-transferase-fusion protein expressed in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C122A
human adenovirus
-
inactive
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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