Information on EC 3.4.22.39 - adenain

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The expected taxonomic range for this enzyme is: unidentified adenovirus

EC NUMBER
COMMENTARY
3.4.22.39
-
RECOMMENDED NAME
GeneOntology No.
adenain
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-/-Xaa- and -Yaa-Xaa-Gly-Xaa-/-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid)
show the reaction diagram
consensus sequences; the GXG consensus site is cleaved more rapidly than the GGX site
-
Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-/-Xaa- and -Yaa-Xaa-Gly-Xaa-/-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid)
show the reaction diagram
consensus sequences
-
Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-/-Xaa- and -Yaa-Xaa-Gly-Xaa-/-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid)
show the reaction diagram
mechanism
-
Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-/-Xaa- and -Yaa-Xaa-Gly-Xaa-/-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid)
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ad L3 23K proteinase
-
-
adenovirus endopeptidase
-
-
-
-
adenovirus type 2 protease
-
-
-
-
cysteine endopeptidase
-
-
-
-
cysteine endoprotease
-
-
-
-
cysteine peptidase
-
-
-
-
cysteine protease
-
-
-
-
cysteine proteinase
-
-
-
-
Endoprotease
-
-
-
-
L-cysteine proteinase
-
-
-
-
L3
-
-
-
-
L3/p23
-
-
-
-
Late L3 23 kDa protein
-
-
-
-
mercapto proteinase
-
-
-
-
oryzain
-
-
-
-
p23
-
-
-
-
papain-like cysteine protease
-
-
-
-
sulfhydryl esterase
-
-
-
-
sulfhydryl protease
-
-
-
-
sulfhydryl proteinase
-
-
-
-
thiol endopeptidase
-
-
-
-
thiol endoproteinase
-
-
-
-
thiol protease
-
-
-
-
thiol proteinase
-
-
-
-
thioprotease
-
-
-
-
trigger peptidase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37353-41-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
human adenovirus
-
-
-
Manually annotated by BRENDA team
serotype 12; type 2
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Ile-Arg-Gly-Gly-NH)2-rhodamine
?
show the reaction diagram
-
-
-
-
?
(Leu-Arg-Gly-Gly)2-rhodamine + H2O
?
show the reaction diagram
-
-
-
-
?
(Leu-Arg-Gly-Gly-NH)2-rhodamine + H2O
?
show the reaction diagram
human adenovirus
-
-
-
?
(Leu-Arg-Gly-Gly-NH)2-rhodamine + H2O
?
show the reaction diagram
human adenovirus
-
-
-
?
(Leu-Arg-Gly-Gly-NH)2-rhodamine + H2O
?
show the reaction diagram
human adenovirus
-
-
-
?
(N-carbobenzyloxy-Met-Arg-Gly-Gly-NH)2-rhodamine
?
show the reaction diagram
-
-
-
-
?
(N-carbobenzyloxy-Nle-Arg-Gly-Gly-NH)2-rhodamine
?
show the reaction diagram
-
-
-
-
?
(Nle-Arg-Gly-Gly-NH)2-rhodamine
?
show the reaction diagram
-
-
-
-
?
actin
36 kD protein
show the reaction diagram
-
-
-
?
core polypeptide PVII
polypeptide VII
show the reaction diagram
-
-
-
?
core polypeptide PVII
polypeptide VII
show the reaction diagram
-
-
-
?
core polypeptide PVII
polypeptide VII
show the reaction diagram
-
-
-
?
epsilon-N-5-carboxytetramethylrhodamine-Arg lucifer yellow CH hydrazone
?
show the reaction diagram
-
-
-
-
?
Fibrin + H2O
?
show the reaction diagram
-
-
-
-
?
Leu-Ser-Gly-Gly-Ala-Phe-Ser-Trp
Leu-Ser-Gly-Gly + Ala-Phe-Ser-Trp
show the reaction diagram
-
-
-
?
Ly-AnLRGA-GFSWK-ctmr-R
?
show the reaction diagram
-
-
-
-
?
Ly-AnLRGG-AFSWK-ctmr-R
?
show the reaction diagram
-
-
-
-
?
Met-Glu-Gly-Gly-Ala-Lys-Lys
?
show the reaction diagram
-
-
-
-
?
Met-Phe-Gly-Gly-Ala-Lys-Lys-Arg
?
show the reaction diagram
-
-
-
-
?
ovalbumin
37 kD protein
show the reaction diagram
-
-
-
?
preterminal proteins
?
show the reaction diagram
-
pVI, pVII, pVIII, IIIa, 11K
-
-
-
pVI
protein VI
show the reaction diagram
-
-
-
?
rhodamine 1,10-bis-(L-leucyl-L-arginylglycylglycine amide) tetrahydrochloride
?
show the reaction diagram
-
-
-
-
-
rhodamine 1,10-bis-(L-leucyl-L-arginylglycylglycine amide) tetrahydrochloride
?
show the reaction diagram
-
-
-
-
?
Met-Ser-Gly-Gly-Ala-Phe-Ser-Trp
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme can function as deubiquitinating enzyme in vitro and in vivo. The protein deubiquitination can be as efficient as proteolytic processing of viral proteins
-
?
additional information
?
-
-
required for the synthesis of infectious virus and is a target for antiviral therapy
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
preterminal proteins
?
show the reaction diagram
-
pVI, pVII, pVIII, IIIa, 11K
-
-
-
additional information
?
-
-
the enzyme can function as deubiquitinating enzyme in vitro and in vivo. The protein deubiquitination can be as efficient as proteolytic processing of viral proteins
-
?
additional information
?
-
-
required for the synthesis of infectious virus and is a target for antiviral therapy
-
?
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,2'-(hydroxynitrosohydrazono)bis-ethaneamine
-
NO generated from 2,2'-(hydroxynitrosohydrazono)bis-ethaneamine inhibits
bestatin, L-transepoxy-succinyl-leucylamido-(4-guanido)-butane
-
-
-
bestatin, L-transepoxy-succinyl-leucylamido-(4-guanido)-butane tyrosine
-
-
-
glutamic acid
-
inhibition by displacement of viral DNA from complexes with enzyme
N-[4-[1-(3-aminopropyl)-2-hydroxy-2-nitrosohydrazino]butyl]-1,3-propanediamine
-
NO generated from N-[4-[1-(3-aminopropyl)-2-hydroxy-2-nitrosohydrazino]butyl]-1,3-propanediamine inhibits the enzyme
NaCl
-
inhibition by displacement of viral DNA from complexes with enzyme
NO
-
inhibits adenovirus replication by targeting the adenovirus proteinase, inhibition is reversible with dithiothreitol
p-chloromercuribenzoate
-
-
Phenylmethylsulfonylfluoride
-
-
Soybean trypsin inhibitor
-
-
-
tosyl-lysinechloromethyl ketone
-
-
tosylamide phenylethylchloromethyl ketone
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
GVQSLKRRRCF
-
pVIc; required
GVQSLKRRRCF
-
pVI-CT, disulfide-linked; required
GVQSLKRRRCF
-
required
polyE
-
interaction of enzyme-virion precursor protein pVI with polymers of glutamic acid leads to increase in substrate hydrolysis
-
pVIc
human adenovirus
-
the enzyme requires two cofactors for maximal activity,the 11-amino acid residue peptide from the C-terminus of adenovirus precursor protein and the viral DNA. Influence of mutant pVIcs on Km-value and turnover number with (Leu-Arg-Gly-Gly-NH)2-rhodamine as substrate
pVIc
human adenovirus
-
the enzyme requires two cofactors for maximal activity,the 11-amino acid residue peptide from the C-terminus of adenovirus precursor protein and the viral DNA
pVIc
human adenovirus
-
11-amino acid residue peptide from the C-terminus of adenovirus precursor protein and the viral DNA is required as cofactor
pVIc
-
enzyme is stimulated by the 11-amino acid residue peptide from the C-terminus of adenovirus precursor protein. In virus particles the enzyme is linked to pVIc via a disulfide bond
viral DNA
-
not required in vitro
-
viral DNA
-
required
-
viral DNA
human adenovirus
-
the enzyme requires two cofactors for maximal activity, the 11-amino acid residue peptide from the C-terminus of adenovirus precursor protein and the viral DNA
-
virion precursor protein pVI
-
11 acid peptide
-
virion precursor protein pVI
-
11 acid peptide. Conserved basic motif KRRR of peptide is less important than the N- or C-terminal regions of peptide in formation of the enzyme-peptide heterodimer and in the activity of the complex. Motif acts as nuclear localization signal
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00517
-
(Ile-Arg-Gly-Gly-NH)2-rhodamine
-
-
-
0.0034
-
(Leu-Arg-Gly-Gly-NH)2-rhodamine
human adenovirus
-
pH 8.0, 25C, activation by viral DNA and the 11-amino acid residue peptide from the C-terminus of adenovirus precursor protein and the viral DNA
0.0092
-
(Leu-Arg-Gly-Gly-NH)2-rhodamine
human adenovirus
-
pH 8.0, 25C, activation by viral DNA
0.0099
-
(Leu-Arg-Gly-Gly-NH)2-rhodamine
human adenovirus
-
pH 8.0, 25C, activation by the the 11-amino acid residue peptide from the C-terminus of adenovirus precursor protein and the viral DNA
0.0948
-
(Leu-Arg-Gly-Gly-NH)2-rhodamine
human adenovirus
-
pH 8.0, 25C, in absence of any cofactor
0.00474
-
(N-carbobenzyloxy-Met-Arg-Gly-Gly-NH)2-rhodamine
-
-
-
0.000758
-
(N-carbobenzyloxy-Nle-Arg-Gly-Gly-NH)2-rhodamine
-
-
-
0.0014
-
rhodamine 110,bis-(L-leucyl-L-arginylglycylglycine amide),tetrahydrochloride
-
-
0.00183
-
rhodamine 110,bis-(L-leucyl-L-arginylglycylglycine amide),tetrahydrochloride
-
-
0.005
-
rhodamine 110,bis-(L-leucyl-L-arginylglycylglycine amide),tetrahydrochloride
-
-
0.0024
-
(Nle-Arg-Gly-Gly-NH)2-rhodamine
-
-
-
additional information
-
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide
human adenovirus
-
influence of mutant pVIcs on Km-value with (Leu-Arg-Gly-Gly-NH)2-rhodamine as substrate
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0023
-
(Leu-Arg-Gly-Gly-NH)2-rhodamine
human adenovirus
-
pH 8.0, 25C, in absence of any cofactor
0.0248
-
(Leu-Arg-Gly-Gly-NH)2-rhodamine
human adenovirus
-
pH 8.0, 25C, activation by viral DNA
0.271
-
(Leu-Arg-Gly-Gly-NH)2-rhodamine
human adenovirus
-
pH 8.0, 25C, activation by the the 11-amino acid residue peptide from the C-terminus of adenovirus precursor protein and the viral DNA
2.78
-
(Leu-Arg-Gly-Gly-NH)2-rhodamine
human adenovirus
-
pH 8.0, 25C, activation by viral DNA and the 11-amino acid residue peptide from the C-terminus of adenovirus precursor protein and the viral DNA
3
6
(Leu-Arg-Gly-Gly-NH)2-rhodamine
human adenovirus
-
pH 8.0, 25C, activation by viral DNA and the 11-amino acid residue peptide from the C-terminus of adenovirus precursor protein and the viral DNA
additional information
-
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide
human adenovirus
-
influence of mutant pVIcs on turnover number with (Leu-Arg-Gly-Gly-NH)2-rhodamine as substrate
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00023
-
glutamic acid
-
37C, pH 8.0
0.000329
-
NaCl
-
37C, pH 8.0
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
additional information
-
HeLa cell infected with adenovirus type 2
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Human adenovirus C serotype 2
Human adenovirus C serotype 2
Human adenovirus C serotype 2
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20000
-
-
gel filtration, SDS-PAGE
25500
-
-
gel filtration, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
-
1 * 20000, SDS-PAGE, gel filtration
monomer
-
1 * 25500, SDS-PAGE, gel filtration
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
structure at 1.6-A resolution of the human adenovirus proteinase in a covalent complex with its 11-amino-acid peptide cofactor
human adenovirus
-
hanging-drop vapour-diffusion technique
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli
-
expressed in Sf9/baculovirus cells
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
-
expression in Sf9/baculovirus cells
-
glutathione S-transferase-fusion protein expressed in Escherichia coli
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
required for the synthesis of infectious virus and is a target for antiviral therapy
medicine
-
NO may be a useful antiadenovirus agent, inhibits adenovirus replication by targeting the adenovirus proteinase. Treatment of infectious virus by 2,2'-(hydroxynitrosohydrazono)bis-ethaneamine dramatically decreases viral infectivity