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1-methylpiperidin-4-one + 4-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 44%, 19:81 anti/syn ratio, enantioselectivity: 5%/1% (anti/syn)
-
-
?
acetyl-citrulline-p-nitroanilide + H2O
acetyl-citrulline + 4-nitroaniline
Ficus sp.
-
30% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
B chain of oxidized insulin + H2O
?
-
-
-
-
?
benzoyl-Arg-ethyl ester + Ala-Ala-OH
?
-
-
-
-
?
benzoyl-Arg-ethyl ester + Ala-Asp-OH
?
-
-
-
-
?
benzoyl-Arg-ethyl ester + Ala-Leu-OH
?
-
weak activity
-
-
?
benzoyl-Arg-ethyl ester + Ala-NH2
?
-
-
-
-
?
benzoyl-Arg-ethyl ester + Ala-Pro-OH
?
-
-
-
-
?
benzoyl-Arg-ethyl ester + Arg-NH2
?
-
-
-
-
?
benzoyl-Arg-ethyl ester + Asp-NH2
?
-
weak activity
-
-
?
benzoyl-Arg-ethyl ester + Gly-NH2
?
-
-
-
-
?
benzoyl-Arg-ethyl ester + Leu-NH2
?
-
-
-
-
?
benzoyl-citrulline-p-nitroanilide + H2O
benzoyl-citrulline + 4-nitroaniline
Ficus sp.
-
81% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzoyl-D,L-arginine-p-nitroanilide + H2O
4-nitroaniline + ?
-
-
-
-
?
benzoyl-D-Arg ethyl ester + H2O
benzoyl-D-Arg + ethanol
-
-
-
-
?
benzoyl-DL-Arg-p-nitroanilide + H2O
benzoyl-DL-Arg + 4-nitroaniline
Ficus sp.
-
-
-
-
?
benzoyl-DL-Arg-p-nitroanilide hydrochloride + H2O
benzoyl-DL-Arg + 4-nitroaniline hydrochloride
-
-
-
-
?
benzoyl-Gly-p-nitrophenyl ester + H2O
?
Ficus sp.
-
-
-
-
?
benzoyl-L-argininamide + H2O
benzoyl-L-Arg + NH3
Ficus sp.
-
-
-
-
?
benzoyl-L-arginine 4-nitroanilide + H2O
benzoyl-L-arginine + 4-nitroaniline
Ficus sp.
-
-
-
-
?
benzoyl-Phe-Val-Arg-NH-(4-nitrophenyl) + H2O
benzoyl-Phe-Val-Arg + 4-nitrophenol
-
-
-
-
?
benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin + H2O
benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-Ala-methyl ester + Ala
benzyloxycarbonyl-Ala-Ala + ?
-
-
-
?
benzyloxycarbonyl-Ala-methyl ester + Ala
benzyloxycarbonyl-Ala-D-Gln + ?
-
-
-
?
benzyloxycarbonyl-Ala-methyl ester + Cys(acetamidomethyl)
benzyloxycarbonyl-Ala-Cys(acetamidomethyl) + ?
-
-
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-citrulline-p-nitroanilide + H2O
benzyloxycarbonyl-citrulline + 4-nitroaniline
Ficus sp.
-
75% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-D-Ala-methyl ester + Ala
benzyloxycarbonyl-Ala-D-Ala + ?
-
-
-
?
benzyloxycarbonyl-Gln-methyl ester + Ala
benzyloxycarbonyl-Ala-Gln + ?
-
-
-
?
benzyloxycarbonyl-Gly-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
11% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Gly-Gly-Phe-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
activity is 2.97fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Gly-methyl ester + Ala
benzyloxycarbonyl-Gly-Ala + ?
-
-
-
?
benzyloxycarbonyl-Gly-methyl ester + Cys(acetamidomethyl)
benzyloxycarbonyl-Gly-Cys(acetamidomethyl) + ?
-
-
-
?
benzyloxycarbonyl-Gly-methyl ester + Gln
benzyloxycarbonyl-Gly-Gln + ?
-
-
-
?
benzyloxycarbonyl-Gly-Phe-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
activity is 12.6fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Gly-Pro-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
39% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl) + H2O
benzyloxycarbonyl-Phe-Arg + 4-nitrophenol
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-NH-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-Phe-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
activity is 19.4fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Pro-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
23% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
Boc-Ala-Ala-Gly-4-nitroanilide + H2O
Boc-Ala-Ala-Gly + 4-nitroaniline
-
-
-
-
?
Boc-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
Boc-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
Collagen + H2O
?
Ficus sp.
-
-
-
-
?
cyclohexanone + 3-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 39%, 86:14 anti/syn ratio, enantioselectivity: 81%/14% (anti/syn)
-
-
?
cyclohexanone + 4-cyanobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 21%, 79:21 anti/syn ratio, enantioselectivity: 77%/5% (anti/syn)
-
-
?
Fibrin + H2O
?
Ficus sp.
-
-
-
-
?
Gelatin + H2O
?
Ficus sp.
-
-
-
-
?
Hemoglobin + H2O
?
Ficus sp.
-
-
-
-
?
hippuryl amide + H2O
hippuric acid + NH3
Ficus sp.
-
-
-
-
?
hippuryl methyl ester + H2O
hippuric acid + methanol
Ficus sp.
-
-
-
-
?
insulin B chain + H2O
fragments of insulin B chain
-
hydrolysis of 6 bonds
-
-
?
N-acetyl-D-Phe-Gly 4-nitroanilide + H2O
N-acetyl-D-Phe-Gly + 4-nitroaniline
Ficus sp.
-
the index of stereochemical selectivity for N-acetyl-L-Phe-Gly 4-nitroanilide and N-acetyl-D-Phe-Gly 4-nitroanilide is 93
-
-
?
N-acetyl-L-Phe-Gly 4-nitroanilide + H2O
N-acetyl-L-Phe-Gly + 4-nitroaniline
Ficus sp.
-
the index of stereochemical selectivity for N-acetyl-L-Phe-Gly 4-nitroanilide and N-acetyl-D-Phe-Gly 4-nitroanilide is 93
-
-
?
N-benzoyl-DL-arginine-2-naphthylamide + H2O
N-benzoyl-DL-arginine + 2-naphthylamine
Ficus sp.
-
-
-
-
?
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Ala + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Leu + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Phe + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Tyr + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
N-tert-butyloxycarbonyl-Ala-(1-4)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-(1-5)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-(1-6)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-(2-5)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-m-(guanidinomethyl)phenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-m-guanidinophenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-p-(guanidinomethyl)phenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-Ph + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + phenol
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-L-Ala-p-amidinophenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-L-Ala-L-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-L-Ala-p-guanidinophenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbony-L-Ala-L-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
Nalpha-benzoyl-DL-arginine 4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
Nalpha-Benzoyl-L-Arg + ethanol
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Ala + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester + H2O
?
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Leu p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Leu + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Lys + 4-nitrophenyl
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Phe + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Tyr + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Val p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Val + 4-nitrophenyl
Ficus sp.
-
ficin II
-
-
?
Nalpha-DL-arginine 4-nitroanilide + H2O
Nalpha-L-arginine + 4-nitroaniline
-
-
-
-
?
oxan-2-one + 4-bromobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 29%, 77:23 anti/syn ratio, enantioselectivity: 71%/27% (anti/syn)
-
-
?
oxan-2-one + 4-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 21%, 42:58 anti/syn ratio, enantioselectivity: 60%/24% (anti/syn)
-
-
?
oxan-3-one + 4-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 28%, 54:46 anti/syn ratio, enantioselectivity: 57%/28% (anti/syn)
-
-
?
oxan-4-one + 4-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 31%, 53:47 anti/syn ratio, enantioselectivity: 25%/17% (anti/syn)
-
-
?
p-nitrophenyl N-acetylglycinate + H2O
p-nitrophenol + N-acetylglycine
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl N-benzoylglycinate + H2O
p-nitrophenol + N-benzoylglycine
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl N-benzyloxycarbonylglycinate + H2O
p-nitrophenol + N-benzyloxycarbonylglycine
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl N-formylglycinate + H2O
p-nitrophenol + N-formylglycine
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl N-trans-cinnamoylglycinate + H2O
p-nitrophenol + N-trans-cinnamoylglycine
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl-carbobenzoxyglycinate + H2O
?
Ficus sp.
-
-
-
-
?
Phe-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
activity is 3.8fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
soy proteins + H2O
?
Ficus sp.
-
-
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 3-bromobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 25%, 47:53 anti/syn ratio, enantioselectivity: 51%/45% (anti/syn)
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 3-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 29%, 51:49 anti/syn ratio, enantioselectivity: 59%/45% (anti/syn)
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 4-bromobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 35%, 75:25 anti/syn ratio, enantioselectivity: 67%/36% (anti/syn)
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 4-nitrobenzaldehyde
tert-butyl 3-[hydroxy(4-nitrophenyl)methyl]-4-oxopiperidine-1-carboxylate
tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide + H2O
tert-butyloxycarbonyl-L-Ala-L-Ala-Gly + 4-nitroaniline
-
-
-
-
?
thian-4-one + 3-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 26%, 86:14 anti/syn ratio, enantioselectivity: 77%/1% (anti/syn)
-
-
?
thian-4-one + 4-cyanobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 23%, 84:16 anti/syn ratio, enantioselectivity: 76%/53% (anti/syn)
-
-
?
thiolan-3-one + 4-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 31%, 40:60 anti/syn ratio, enantioselectivity: 45%/23% (anti/syn)
-
-
?
additional information
?
-
casein + H2O
?
-
-
-
-
?
casein + H2O
?
-
hydrolysis ratio is 13% at 48 h
-
-
?
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
Nalpha-Benzoyl-L-Arg + ethanol
-
-
-
-
?
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
Nalpha-Benzoyl-L-Arg + ethanol
Ficus sp.
-
-
-
-
?
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
Ficus sp.
-
-
-
-
?
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 4-nitrobenzaldehyde
tert-butyl 3-[hydroxy(4-nitrophenyl)methyl]-4-oxopiperidine-1-carboxylate
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 36%, 41:59 anti/syn ratio, enantioselectivity: 60%/14% (anti/syn)
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 4-nitrobenzaldehyde
tert-butyl 3-[hydroxy(4-nitrophenyl)methyl]-4-oxopiperidine-1-carboxylate
-
the catalytic activity and stereoselectivity of ficin are significantly influenced by reaction media. Best yield of 34% with a poor enantioselectivity of 29% in THF. Best enantioselectivity of 57% with a yield of 28% in MeCN. In tested polar solvents including i-PrOH, DMSO, and DMF, ficin shows lower activityand enantioselectivity
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 4-nitrobenzaldehyde
tert-butyl 3-[hydroxy(4-nitrophenyl)methyl]-4-oxopiperidine-1-carboxylate
-
water content is important for enantioselectivity and enzymatic activity. When water content is at 0.15 (H2O/(H2O+MeCN): 0.15,v/v) the reaction give the best enantioselectivity of 59% with 32% yield
-
-
?
additional information
?
-
-
destruction of Staphylococcus aureus and Staphylococcus epidermidis biofilms with ficin
-
-
?
additional information
?
-
-
a wide variety of peptide bonds is hydrolyzed, but peptide bonds following an aromatic residue appear to be hydrolyzed more efficiently than the other
-
-
?
additional information
?
-
-
ficain is the major proteolytic activity component in the milky latex of the plant
-
-
?
additional information
?
-
-
substrate specificity compared to papain, the ficain S1-pocket accepts Gly, SEr, Glu, Tyr, and Phe, the S2-pocket accepts cysteic acid, Val, Leu, Gly, and Phe, Arg-NH-nitrophenyl is a poor substrate
-
-
?
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3.3
benzoyl-DL-Arg-p-nitroanilide
Ficus sp.
-
-
0.0248 - 0.189
benzoyl-Gly-p-nitrophenyl ester
0.0024
benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin
-
pH 6.8, 37°C
0.3
benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl)
-
pH 6.8, 37°C
0.0137
casein
-
pH 7.0, 60°C
0.2
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester
Ficus sp.
-
ficin II
0.024
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester
Ficus sp.
-
ficin II
0.128
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester
Ficus sp.
-
ficin II
0.012
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester
Ficus sp.
-
ficin II
21.3
Nalpha-benzoyl-L-Arg ethyl ester
Ficus sp.
-
-
0.0059 - 0.0258
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
0.0125 - 0.0598
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
0.0172
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0087 - 0.052
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
0.0377
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0034
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0327
Nalpha-carbobenzoxy-Val p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.309 - 0.328
p-nitrophenyl N-acetylglycinate
0.0214 - 0.025
p-nitrophenyl N-benzoylglycinate
0.0183 - 0.0194
p-nitrophenyl N-benzyloxycarbonylglycinate
1.1
p-nitrophenyl N-formylglycinate
Ficus sp.
-
ficin III
0.0067 - 0.0076
p-nitrophenyl N-trans-cinnamoylglycinate
additional information
additional information
-
0.0248
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 6.6
0.0272
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 5.61
0.0283
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 5.3
0.0513
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 3.91
0.189
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 3.15
0.0059
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
0.0122
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0258
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0125
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
-
0.0134
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
0.0598
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0087
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
0.052
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.309
p-nitrophenyl N-acetylglycinate
Ficus sp.
-
ficin III
0.328
p-nitrophenyl N-acetylglycinate
Ficus sp.
-
ficin II
0.0214
p-nitrophenyl N-benzoylglycinate
Ficus sp.
-
ficin III
0.025
p-nitrophenyl N-benzoylglycinate
Ficus sp.
-
ficin II
0.0183
p-nitrophenyl N-benzyloxycarbonylglycinate
Ficus sp.
-
ficin III
0.0194
p-nitrophenyl N-benzyloxycarbonylglycinate
Ficus sp.
-
ficin II
0.0067
p-nitrophenyl N-trans-cinnamoylglycinate
Ficus sp.
-
ficin III
0.0076
p-nitrophenyl N-trans-cinnamoylglycinate
Ficus sp.
-
ficin II
additional information
additional information
-
-
-
additional information
additional information
-
little or no effect of temperature on Km
-
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0.0096
benzoyl-DL-Arg-p-nitroanilide
Ficus sp.
-
-
2.56 - 3.41
benzoyl-Gly-p-nitrophenyl ester
32.9
casein
-
pH 7.0, 60°C
0.45
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester
Ficus sp.
-
ficin II
1.85
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester
Ficus sp.
-
ficin II
1.2
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester
Ficus sp.
-
ficin II
0.92
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester
Ficus sp.
-
ficin II
1.29
Nalpha-benzoyl-L-Arg ethyl ester
Ficus sp.
-
-
29.8 - 31
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
1.85 - 12.4
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
7.45
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
4.5
Nalpha-carbobenzoxy-Leu p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
35 - 55
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
2.83
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
2.27
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
1.02
Nalpha-carbobenzoxy-Val p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
2.3 - 2.84
p-nitrophenyl N-acetylglycinate
7.72 - 53.6
p-nitrophenyl N-benzoylglycinate
13.3 - 95.7
p-nitrophenyl N-benzyloxycarbonylglycinate
2.5
p-nitrophenyl N-formylglycinate
Ficus sp.
-
ficin II
86.4 - 123
p-nitrophenyl N-trans-cinnamoylglycinate
2.56
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 3.91
3.04
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 3.15
3.28
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 5.61 and at pH 6.6
3.41
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 5.3
29.8
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
31
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
1.85
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
-
9.25
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
12.4
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
35
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
55
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
2.3
p-nitrophenyl N-acetylglycinate
Ficus sp.
-
ficin II
2.84
p-nitrophenyl N-acetylglycinate
Ficus sp.
-
ficin III
7.72
p-nitrophenyl N-benzoylglycinate
Ficus sp.
-
ficin III
53.6
p-nitrophenyl N-benzoylglycinate
Ficus sp.
-
ficin II
13.3
p-nitrophenyl N-benzyloxycarbonylglycinate
Ficus sp.
-
ficin III
95.7
p-nitrophenyl N-benzyloxycarbonylglycinate
Ficus sp.
-
ficin III
86.4
p-nitrophenyl N-trans-cinnamoylglycinate
Ficus sp.
-
ficin II
123
p-nitrophenyl N-trans-cinnamoylglycinate
Ficus sp.
-
ficin III
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Gray, C.J.; Boukouvalas, J.; Szawelski, R.J.; Wharton, C.W.
Benzyloxycarbonylphenylalanylcitrulline p-nitroanilide as a substrate for papain and other plant cysteine proteinases
Biochem. J.
219
325-328
1984
Ficus sp.
brenda
Wakamatsu, N.; Kominami, E.; Katunuma, N.
Comparison of properties of thiol proteinase inhibitors from rat serum and liver
J. Biol. Chem.
257
14653-14656
1982
Ficus sp.
brenda
Rodis, P.; Hoff, J.E.
Naturally occurring protein crystals in the potato. Inhibitor of papain, chymopapain, and ficin
Plant Physiol.
74
907-911
1984
Ficus sp.
brenda
Lynn, K.R.; Clevette-Radford, N.A.
Ficin E, a serine-centred protease from Ficus elastica
Phytochemistry
25
1559-1561
1986
Ficus elastica
-
brenda
Minakata, K.; Asano, M.
Acidic cysteine proteinase inhibitor in seminal plasma
Biol. Chem. Hoppe-Seyler
366
15-18
1985
Ficus sp.
brenda
Friedenson, B.; Liener, I.E.
Evidence that ficin is a glycoprotein
Biochim. Biophys. Acta
342
209-212
1974
Ficus sp.
brenda
Anderson, C.D.; Hall, P.L.
Purification of ficin by affinity chromatography
Anal. Biochem.
60
417-423
1974
Ficus sp.
brenda
Hall, P.L.; Anderson, C.D.
Proflavine interactions with papain and ficin. II. Effects of dye binding upon reversible inhibition
Biochemistry
13
2087-2092
1974
Ficus sp.
brenda
Hall, P.L.; Anderson, C.D.
Proflavine interactions with papain and ficin. I. Dye binding and its effects upon enzyme inactivation by N-alkylmaleimides
Biochemistry
13
2082-2087
1974
Ficus sp.
brenda
Kortt, A.A.; Hinds, J.A.; Zerner, B.
On the specificity and pH dependence of ficin-catalyzed hydrolyses. Some comparisons with bromelain specificity
Biochemistry
13
2029-2037
1974
Ficus sp.
brenda
Kortt, A.A.; Hamilton, S.; Webb, E.C.; Zerner, B.
Ficins (EC 3.4.22.3). Purification and characterization of the enzymatic components of the latex of Ficus glabrata
Biochemistry
13
2023-2028
1974
Ficus glabrata
brenda
Sugiura, M.; Sasaki, M.
Studies on proteinases from Ficus carica var. Horaishi. V. Purification and properties of a sugar-containing proteinase (ficin S)
Biochim. Biophys. Acta
350
38-47
1974
Ficus carica
brenda
Sasaki, M.; Yamamoto, H.; Yamamoto, H.; Iida, S.
Interaction of human serum proteinase inhibitors with proteolytic enzymes of animal, plant, and bacterial origin
J. Biochem.
75
171-177
1974
Ficus sp.
brenda
Bjrk, I.; Ylinenjrvi, K.
Interaction between chicken cystatin and the cysteine proteinases actinidin, chymopapain A, and ficin
Biochemistry
29
1770-1776
1990
Ficus sp.
brenda
Hollaway, M.R.; Antonini, E.; Brunori, M.
The pH-dependence of rates of individual steps in ficin catalysis
Eur. J. Biochem.
24
332-341
1971
Ficus sp.
brenda
Whitaker, J.R.; Lee, L.S.
Ficin- and papain-catalyzed reactions. Effect of temperature on reactivity of the essential sulfhydryl group of ficin in the presence and absence of competitive inhibitors
Arch. Biochem. Biophys.
148
208-216
1972
Ficus glabrata
brenda
Jones, I.K.; Glazer, A.N.
Comparative studies on four sulfhydryl endopeptidases (ficins) of Ficus glabrata latex
J. Biol. Chem.
245
2765-2772
1970
Ficus glabrata
brenda
Monter, B.; Herzog, B.; Stehle, P.; Furst, P.
Kinetically controlled synthesis of dipeptides using ficin as biocatalyst
Biotechnol. Appl. Biochem.
14
183-181
1991
Ficus carica
brenda
Williams, D.C.; Whitaker, J.R.
Multiple molecular forms of Ficus glabrata ficin. Their separation and relative physical, chemical, and enzymatic properties
Plant Physiol.
44
1574-1583
1969
Ficus glabrata
brenda
Englund, P.T.; King, T.P.; Craig, L.C.; Walti, A.
Studies on ficin. I. Its isolation and characterization
Biochemistry
7
163-175
1968
Ficus glabrata
brenda
Metrione, R.M.; Johnston, R.B.; Seng, R.
Purification, partial characterization and sequence around a reactive sulfhydryl of ficin
Arch. Biochem. Biophys.
122
137-143
1967
Ficus sp.
brenda
Hnsler, M.; Ullmann, G.; Jakubke, H.D.
The Application of papain, ficin and clostripain in kinetically controlled peptide synthesis in frozen aqueous solutions
J. Pept. Sci.
1
283-287
1995
Ficus carica
brenda
Liener, I.E.; Friedenson, B.
Ficin
Methods Enzymol.
19
261-273
1970
Ficus anthelmintica, Ficus carica, Ficus glabrata, Ficus sp.
-
brenda
Patel, M.; Kayani, I.S.; Mellor, G.W.; Sreedharan, S.; Templeton, W.; Thomas, E.W.; Thomas, M.; Brocklehurst, K.
Variation in the P2-S2 stereochemical selectivity towards the enantiomeric N-acetylphenylalanylglycine 4-nitroanilides among the cysteine proteinases papain, ficin and actinidin
Biochem. J.
281
553-559
1992
Ficus sp.
brenda
Mazda, T.; Makino, K.; Yabe, R.; Nakata, K.; Fujisawa, K.; Ohshima, H.
Use of standarized protease enzymes for antibody screening of blood donor samples with the microplate system
Transfus. Med.
5
43-50
1995
Ficus sp.
brenda
Mariani, M.; Camagna, M.; Tarditi, L.; Seccamani, E.
A new enzymatic method to obtain high-yield F(ab)2 suitable for clinical use from mouse IgG1
Mol. Immunol.
28
69-77
1991
Ficus sp.
brenda
Ruemenapf, T.; Thiel, H.J.
434. Cathepsin T
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2nd Ed.
1404
2004
Ficus glabrata
-
brenda
Sekizaki, H.; Toyota, E.; Fuchise, T.; Zhou, S.; Noguchi, Y.; Horita, K.
Application of several types of substrates to ficin-catalyzed peptide synthesis
Amino Acids
34
149-153
2008
Ficus sp.
brenda
Devaraj, K.B.; Kumar, P.R.; Prakash, V.
Purification, characterization, and solvent-induced thermal stabilization of ficin from Ficus carica
J. Agric. Food Chem.
56
11417-11423
2008
Ficus carica
brenda
Devaraj, K.B.; Kumar, P.R.; Prakash, V.
Characterization of acid-induced molten globule like state of ficin
Int. J. Biol. Macromol.
45
248-254
2009
Ficus carica
brenda
Valdes-Rodriguez, S.; Cedro-Tanda, A.; Aguilar-Hernandez, V.; Cortes-Onofre, E.; Blanco-Labra, A.; Guerrero-Rangel, A.
Recombinant amaranth cystatin (AhCPI) inhibits the growth of phytopathogenic fungi
Plant Physiol. Biochem.
48
469-475
2010
Ficus sp.
brenda
Sidek, N.; Halim, A.; Tayyab, S.
Denatured states of ficin induced by urea and guanidine hydrochloride
Turk. J. Biochem.
35
45-49
2010
Ficus sp.
-
brenda
Azarkan, M.; Matagne, A.; Wattiez, R.; Bolle, L.; Vandenameele, J.; Baeyens-Volant, D.
Selective and reversible thiol-pegylation, an effective approach for purification and characterization of five fully active ficin (iso)forms from Ficus carica latex
Phytochemistry
72
1718-1731
2011
Ficus carica
brenda
Devaraj, K.; Kumar, P.; Prakash, V.
Comparison of activity and conformational changes of ficin during denaturation by urea and guanidine hydrochloride
Process Biochem.
46
458-464
2011
Ficus carica
-
brenda
Haesaerts, S.; Rodriguez Buitrago, J.; Loris, R.; Baeyens-Volant, D.; Azarkan, M.
Crystallization and preliminary X-ray analysis of four cysteine proteases from Ficus carica latex
Acta Crystallogr. Sect. F
71
459-465
2015
Ficus carica
brenda
Fu, J.; Gao, N.; Yang, Y.; Guan, Z.; He, Y.
Ficin-catalyzed asymmetric aldol reactions of heterocyclic ketones with aldehydes
J. Mol. Catal. B
97
1-4
2013
Ficus carica
-
brenda
Raskovic, B.; Lazic, J.; Polovic, N.
Characterisation of general proteolytic, milk clotting and antifungal activity of Ficus carica latex during fruit ripening
J. Sci. Food Agric.
96
576-582
2015
Ficus carica
brenda
Zare, H.; Moosavi-Movahedi, A.A.; Salami, M.; Mirzaei, M.; Saboury, A.A.; Sheibani, N.
Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex
Phytochemistry
87
16-22
2013
Ficus carica
brenda
Gagaoua, M.; Boucherba, N.; Bouanane-Darenfed, A.; Ziane, F.; Nait-Rabah, S.; Hafid, K.; Boudechicha, H.
Three-phase partitioning as an efficient method for the purification and recovery of ficin from Mediterranean fig (Ficus carica L.) latex
Sep. Purif. Technol.
132
461-467
2014
Ficus carica
-
brenda
Milosevic, J.; Jankovic, B.; Prodanovic, R.; Polovic, N.
Comparative stability of ficin and papain in acidic conditions and the presence of ethanol
Amino Acids
51
829-838
2019
Ficus carica
brenda
Homaei, A.; Stevanato, R.; Etemadipour, R.; Hemmati, R.
Purification, catalytic, kinetic and thermodynamic characteristics of a novel ficin from Ficus johannis
Biocatal. Agricult. Biotechnol.
10
360-366
2017
Ficus johannis
-
brenda
Zare, H.; Moosavi-Movahedi, A.A.; Salami, M.; Sheibani, N.; Khajeh, K.; Habibi-Rezaei, M.
Autolysis control and structural changes of purified ficin from Iranian fig latex with synthetic inhibitors
Int. J. Biol. Macromol.
84
464-471
2016
Ficus
brenda
Baeyens-Volant, D.; Matagne, A.; El Mahyaoui, R.; Wattiez, R.; Azarkan, M.
A novel form of ficin from Ficus carica latex Purification and characterization
Phytochemistry
117
154-167
2015
Ficus carica
brenda
Baidamshina, D.R.; Trizna, E.Y.; Holyavka, M.G.; Bogachev, M.I.; Artyukhov, V.G.; Akhatova, F.S.; Rozhina, E.V.; Fakhrullin, R.F.; Kayumov, A.R.
Targeting microbial biofilms using Ficin, a nonspecific plant protease
Sci. Rep.
7
46068
2017
Ficus
brenda