Information on EC 3.4.22.3 - ficain

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The expected taxonomic range for this enzyme is: Ficus

EC NUMBER
COMMENTARY hide
3.4.22.3
-
RECOMMENDED NAME
GeneOntology No.
ficain
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Similar to that of papain
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
9001-33-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Ficus anthelmintica
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Ficus sp.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-methylpiperidin-4-one + 4-nitrobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 44%, 19:81 anti/syn ratio, enantioselectivity: 5%/1% (anti/syn)
-
-
?
acetyl-citrulline-p-nitroanilide + H2O
acetyl-citrulline + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
30% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
B chain of oxidized insulin + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Ala-Ala-OH
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Ala-Asp-OH
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Ala-Leu-OH
?
show the reaction diagram
-
weak activity
-
-
?
benzoyl-Arg-ethyl ester + Ala-NH2
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Ala-Pro-OH
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Arg-NH2
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Asp-NH2
?
show the reaction diagram
-
weak activity
-
-
?
benzoyl-Arg-ethyl ester + Gly-NH2
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Leu-NH2
?
show the reaction diagram
-
-
-
-
?
benzoyl-citrulline-p-nitroanilide + H2O
benzoyl-citrulline + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
81% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzoyl-D,L-arginine-p-nitroanilide + H2O
4-nitroaniline + ?
show the reaction diagram
-
-
-
-
?
benzoyl-D-Arg ethyl ester + H2O
benzoyl-D-Arg + ethanol
show the reaction diagram
-
-
-
-
?
benzoyl-DL-Arg-p-nitroanilide + H2O
benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
-
-
-
?
benzoyl-DL-Arg-p-nitroanilide hydrochloride + H2O
benzoyl-DL-Arg + 4-nitroaniline hydrochloride
show the reaction diagram
-
-
-
-
?
benzoyl-Gly-p-nitrophenyl ester + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
benzoyl-L-argininamide + H2O
benzoyl-L-Arg + NH3
show the reaction diagram
Ficus sp.
-
-
-
-
?
benzoyl-L-arginine 4-nitroanilide + H2O
benzoyl-L-arginine + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
-
-
-
?
benzoyl-Phe-Val-Arg-NH-(4-nitrophenyl) + H2O
benzoyl-Phe-Val-Arg + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin + H2O
benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-methyl ester + Ala
benzyloxycarbonyl-Ala-Ala + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-methyl ester + Ala
benzyloxycarbonyl-Ala-D-Gln + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-methyl ester + Cys(acetamidomethyl)
benzyloxycarbonyl-Ala-Cys(acetamidomethyl) + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-citrulline-p-nitroanilide + H2O
benzyloxycarbonyl-citrulline + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
75% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-D-Ala-methyl ester + Ala
benzyloxycarbonyl-Ala-D-Ala + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gln-methyl ester + Ala
benzyloxycarbonyl-Ala-Gln + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
11% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Gly-Gly-Phe-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
activity is 2.97fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Gly-methyl ester + Ala
benzyloxycarbonyl-Gly-Ala + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-methyl ester + Cys(acetamidomethyl)
benzyloxycarbonyl-Gly-Cys(acetamidomethyl) + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-methyl ester + Gln
benzyloxycarbonyl-Gly-Gln + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-Phe-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
activity is 12.6fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Gly-Pro-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
39% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl) + H2O
benzyloxycarbonyl-Phe-Arg + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-NH-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
activity is 19.4fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Pro-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
23% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
casein + H2O
?
show the reaction diagram
Collagen + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
cyclohexanone + 3-nitrobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 39%, 86:14 anti/syn ratio, enantioselectivity: 81%/14% (anti/syn)
-
-
?
cyclohexanone + 4-cyanobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 21%, 79:21 anti/syn ratio, enantioselectivity: 77%/5% (anti/syn)
-
-
?
Fibrin + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
Hemoglobin + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
hippuryl amide + H2O
hippuric acid + NH3
show the reaction diagram
Ficus sp.
-
-
-
-
?
hippuryl methyl ester + H2O
hippuric acid + methanol
show the reaction diagram
Ficus sp.
-
-
-
-
?
insulin B chain + H2O
fragments of insulin B chain
show the reaction diagram
-
hydrolysis of 6 bonds
-
-
?
N-acetyl-D-Phe-Gly 4-nitroanilide + H2O
N-acetyl-D-Phe-Gly + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
the index of stereochemical selectivity for N-acetyl-L-Phe-Gly 4-nitroanilide and N-acetyl-D-Phe-Gly 4-nitroanilide is 93
-
-
?
N-acetyl-L-Phe-Gly 4-nitroanilide + H2O
N-acetyl-L-Phe-Gly + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
the index of stereochemical selectivity for N-acetyl-L-Phe-Gly 4-nitroanilide and N-acetyl-D-Phe-Gly 4-nitroanilide is 93
-
-
?
N-benzoyl-DL-arginine-2-naphthylamide + H2O
N-benzoyl-DL-arginine + 2-naphthylamine
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Ala + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Leu + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Phe + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Tyr + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
N-tert-butyloxycarbonyl-Ala-(1-4)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-(1-5)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-(1-6)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-(2-5)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-m-(guanidinomethyl)phenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-m-guanidinophenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-p-(guanidinomethyl)phenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-Ph + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + phenol
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-L-Ala-p-amidinophenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-L-Ala-L-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-L-Ala-p-guanidinophenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbony-L-Ala-L-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
Nalpha-Benzoyl-L-Arg + ethanol
show the reaction diagram
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Ala + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
show the reaction diagram
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester + H2O
?
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Leu p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Leu + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Lys + 4-nitrophenyl
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Phe + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Tyr + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Val p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Val + 4-nitrophenyl
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-DL-arginine 4-nitroanilide + H2O
Nalpha-L-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
oxan-2-one + 4-bromobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 29%, 77:23 anti/syn ratio, enantioselectivity: 71%/27% (anti/syn)
-
-
?
oxan-2-one + 4-nitrobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 21%, 42:58 anti/syn ratio, enantioselectivity: 60%/24% (anti/syn)
-
-
?
oxan-3-one + 4-nitrobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 28%, 54:46 anti/syn ratio, enantioselectivity: 57%/28% (anti/syn)
-
-
?
oxan-4-one + 4-nitrobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 31%, 53:47 anti/syn ratio, enantioselectivity: 25%/17% (anti/syn)
-
-
?
p-nitrophenyl-carbobenzoxyglycinate + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
p-nitrophenyl-N-trans-acetyl-glycinate + H2O
?
show the reaction diagram
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl-N-trans-benzoyl-glycinate + H2O
?
show the reaction diagram
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl-N-trans-benzyloxycarbonyl-glycinate + H2O
?
show the reaction diagram
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl-N-trans-cinnamoyl-glycinate + H2O
?
show the reaction diagram
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl-N-trans-formyl-glycinate + H2O
?
show the reaction diagram
Ficus sp.
-
ficin II and III
-
-
?
Phe-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
activity is 3.8fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
soy proteins + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 3-bromobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 25%, 47:53 anti/syn ratio, enantioselectivity: 51%/45% (anti/syn)
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 3-nitrobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 29%, 51:49 anti/syn ratio, enantioselectivity: 59%/45% (anti/syn)
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 4-bromobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 35%, 75:25 anti/syn ratio, enantioselectivity: 67%/36% (anti/syn)
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 4-nitrobenzaldehyde
tert-butyl 3-[hydroxy(4-nitrophenyl)methyl]-4-oxopiperidine-1-carboxylate
show the reaction diagram
tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide + H2O
tert-butyloxycarbonyl-L-Ala-L-Ala-Gly + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
thian-4-one + 3-nitrobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 26%, 86:14 anti/syn ratio, enantioselectivity: 77%/1% (anti/syn)
-
-
?
thian-4-one + 4-cyanobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 23%, 84:16 anti/syn ratio, enantioselectivity: 76%/53% (anti/syn)
-
-
?
thiolan-3-one + 4-nitrobenzaldehyde
?
show the reaction diagram
-
reaction condition: 30C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 31%, 40:60 anti/syn ratio, enantioselectivity: 45%/23% (anti/syn)
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
casein + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
ficain is the major proteolytic activity component in the milky latex of the plant
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha2-Macroglobulin
Ficus sp.
-
-
-
amaranth cystatin
Ficus sp.
-
Amaranthus hypochondracus cysteine proteinase inhibitor, GenBank accession number DQ792503
-
Benzamidoacetonitrile
Ficus sp.
-
-
Chloroacetamide
Ficus sp.
-
-
Cystatin
cysteine proteinase inhibitor in human seminal plasma
Ficus sp.
-
-
-
diisopropyl fluorophosphate
-
-
E-64
-
complete inhibition of all five ficin isoforms at 0.1 mM
H2O2
-
-
inhibitors from rat and serum liver
Ficus sp.
-
-
-
iodoacetamide
iodoacetic acid
methylmethanethiol sulfonate
-
complete inhibition of all five ficin isoforms at 3 mM
Na2S4O6
-
-
-
Nalpha-tosyl-L-lysine chloromethyl ketone
-
complete inhibition of all five ficin isoforms at 0.1 mM
Nalpha-tosyl-L-phenylalanine chloromethylketone
-
complete inhibition of all five ficin isoforms at 0.1 mM
naturally occuring protein crystal in potato
Ficus sp.
-
-
-
NEM
Ficus sp.
-
-
p-chloromercuribenzoate
-
activity is completely inhibited by the cysteine-group specific inhibitor
PCMB
-
-
phenylmethane sulfonyl fluoride
-
-
potassium tetrathionate
-
-
Proflavine
Sodium tetrathionate
-
activity is completely inhibited by the cysteine-group specific inhibitor
additional information
-
all five ficin isoforms are not inhibited by pepstatin A, Pafabloc SC, and EDTA
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cys
-
activates
cysteine
-
the enzyme requires activation by reducing agents, optimal at 4 mM
EDTA
Ficus sp.
-
enhances activity
mercaptoethanol
-
activates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.3
benzoyl-DL-Arg-p-nitroanilide
Ficus sp.
-
-
0.0248 - 0.189
benzoyl-Gly-p-nitrophenyl ester
0.0024
benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin
-
pH 6.8, 37C
0.3
benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl)
-
pH 6.8, 37C
0.2
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester
Ficus sp.
-
ficin II
0.024
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester
Ficus sp.
-
ficin II
0.128
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester
Ficus sp.
-
ficin II
0.012
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester
Ficus sp.
-
ficin II
21.3
Nalpha-benzoyl-L-Arg ethyl ester
Ficus sp.
-
-
0.0059 - 0.0258
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
0.0125 - 0.0598
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
0.0172
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0087 - 0.052
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
0.0377
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0034
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0327
Nalpha-carbobenzoxy-Val p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.309 - 0.328
p-nitrophenyl-N-trans-acetyl-glycinate
0.0214 - 0.025
p-nitrophenyl-N-trans-benzoyl-glycinate
0.0183 - 0.0194
p-nitrophenyl-N-trans-benzyloxycarbonyl-glycinate
0.0067 - 0.0076
p-nitrophenyl-N-trans-cinnamoyl-glycinate
1.1
p-nitrophenyl-N-trans-formyl-glycinate
Ficus sp.
-
ficin III
-
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0096
benzoyl-DL-Arg-p-nitroanilide
Ficus sp.
-
-
2.56 - 3.41
benzoyl-Gly-p-nitrophenyl ester
0.45
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester
Ficus sp.
-
ficin II
1.85
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester
Ficus sp.
-
ficin II
1.2
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester
Ficus sp.
-
ficin II
0.92
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester
Ficus sp.
-
ficin II
1.29
Nalpha-benzoyl-L-Arg ethyl ester
Ficus sp.
-
-
29.8 - 31
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
1.85 - 12.4
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
7.45
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
4.5
Nalpha-carbobenzoxy-Leu p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
35 - 55
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
2.83
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
2.27
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
1.02
Nalpha-carbobenzoxy-Val p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
2.3 - 2.84
p-nitrophenyl-N-trans-acetyl-glycinate
7.72 - 53.6
p-nitrophenyl-N-trans-benzoyl-glycinate
13.3 - 95.7
p-nitrophenyl-N-trans-benzyloxycarbonyl-glycinate
86.4 - 123
p-nitrophenyl-N-trans-cinnamoyl-glycinate
2.5
p-nitrophenyl-N-trans-formyl-glycinate
Ficus sp.
-
ficin II
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.325
amaranth cystatin
Ficus sp.
-
Amaranthus hypochondracus cysteine proteinase inhibitor
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.044
-
purified isoform ficin A, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.046
-
purified isoform ficin B, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.073
-
purified isoform ficin C, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.11
-
purified isoform ficin D2, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.117
-
purified isoform ficin D1, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.127
-
purified isoform ficin A, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.146
-
purified isoform ficin D1, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.154
-
purified isoform ficin D2, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.186
-
purified isoform ficin C, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.204
-
purified isoform ficin B, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
6824
-
purifed enzyme against casein
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6
-
assay at
9
Ficus sp.
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
-
pH 5.0: about 50% of maximal activity, pH 9.0: about 55% of maximal activity
6 - 11
-
pH 6.0: about 50% of maximal activity, pH 11.0: about 35% of maximal activity, hydrolysis of casein
6.5 - 8.5
-
maximum activity is observed between pH 6.5 and 8.5
additional information
-
pH profile
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
30
-
best enantioselectivity of 60% at 30C
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
unripe, the enzyme is located in the latex milk secreted by the organ
Manually annotated by BRENDA team
-
the enzyme is located in the latex milk secreted by the organ
Manually annotated by BRENDA team
-
the enzyme is located in the latex milk secreted by the organ
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10420 - 12030
-
multiple molecular forms, gel filtration
23100
-
determined by matrix-assisted laser desorption ionization-time of flight mass spectrometry
23400
-
SDS-PAGE
23850
-
ESIQ-TOF mass spectroscopy
24130
-
ESIQ-TOF mass spectroscopy
24280
-
ESIQ-TOF mass spectroscopy
24400
-
ESIQ-TOF mass spectroscopy
25200
-
ficin F, meniscus depletion method
25600
-
ficin H, meniscus depletion method
26100
-
ficin B, meniscus depletion method
26500
Ficus anthelmintica
-
ultracentrifugation
50000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
-
amino acid sequence sand structure analysis
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
side-chain modification
additional information
-
the enzyme contains three disulfide bonds
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
diffracted to better than 2.10 A resolution
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
active at neutral pH and looses its activity completely below pH 3.0
708754
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 70
-
stable for 1 h
60
-
30 min, stable below
67
-
30 min, complete inactivation
70
-
ficin looses 37% of its activity at 70C after 10 min of incubation, and in the presence of co-solvents, the activity is protected up to 91%, maximum protection is shown by trehalose and followed by sorbitol, sucrose, and xylitol; ficin looses nearly 50% of its activity after 20 min of incubation; in the presence of sorbitol, the enzyme retained 82% of its activity after 20 min of incubation; in the presence of sucrose, the enzyme retained 80% of its activity after 20 min of incubation; in the presence of trehalose, the enzyme retained 85% of its activity after 20 min and 53% after 60 min of incubation; in the presence of xylitol, the enzyme retained 78% of its activity after 20 min of incubation
73
-
apparent denaturation temperature of ficin
85
-
the apparent denaturation temperature of ficin shifts in the presence of 40% trehalose
additional information
-
the apparent denaturation temperature of ficin in the presence of 10, 20, 30, and 40% sorbitol is 75, 77, 81 and 83C; the apparent denaturation temperature of ficin in the presence of 10, 20, 30, and 40% sucrose is 74, 77, 80 and 82C; the apparent denaturation temperature of ficin in the presence of 10, 20, 30, and 40% xylitol is 74, 76, 79 and 81C; the apparent thermal denaturation temperature of ficin is significantly increased from the control value of 72C in the presence of all co-solvents
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
9 M urea (1h): loss of about 65% of activity, 6 M guanidine hydrochloride (1h): complete loss of acivity
Ficus sp.
-
autodigestion during purification
-
the maximum stabilization effect is observed in terms of thermal stabilization by the co-solvent trehalose
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
guanidine-HCl
-
ficin loses its complete structure in presence of 4 M guanidine-HCl at pH 7.0, ficin loses 50% activity at around 1 M guanidine-HCl, and is complete inactivated at 3 M
urea
-
the enzyme activity decreases significantly at low concentration of urea before unfolding of the enzyme molecule (about 50% residual activity at 2 M, about 20% residual activity at 4 M, no activity at 6 M)
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
37C, 24 h, enzyme undergoes autolysis
-
4C, 20 days, enzyme undergoes autolysis
-
4C, 50% loss of activity after 4.5 months
-
4C, as salt precipitate, stable for at least 15 months
-
4C, pH 2.0-8.0, stable for 20 h
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a major ficin from the commercial crude proteinase mixture preparation of Ficus carica is purified by using a SP-Sepharose cation-exchanger column on fast protein liquid chromatography
-
commercial preparation
Ficus sp.
-
ficin II and III
-
further purification of a commercial latex preparation by cation exchange chromatography, and 2,2'-dipyridyl disulfide-glutathione or aminophenylmercuric acetate affinity chromatography, or by Gly-Phe-NH-methylcyanide affinity chromatography with elution by 2,2'-dipyridyl disulfide
-
multiple molecular forms
-
SP-Sepharose column chromatography
-
three-phase partitioning is applied for purification and recovery of ficin from Ficus carica latex. The enzyme is purified to 6.04fold with 167% recovery
-
using HPLC chromatography, ultrafiltration, ion-exchange chromatography and gel filtration
-
using ion-exchange chromatography
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
industry
-
enzyme is used in the food, pharmaceutical, and detergent industries
synthesis