Information on EC 3.4.22.3 - ficain

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The expected taxonomic range for this enzyme is: Ficus

EC NUMBER
COMMENTARY
3.4.22.3
-
RECOMMENDED NAME
GeneOntology No.
ficain
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Similar to that of papain
show the reaction diagram
-
-
-
-
Similar to that of papain
show the reaction diagram
active site structure
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
hydrolysis of peptide bond
-
-
endopeptidase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
debricin
-
-
-
-
EC 3.4.4.12
-
-
formerly
-
ficin
-
-
-
-
ficin
Ficus sp.
-
-
ficin A
-
isoform
ficin B
-
isoform
ficin C
-
isoform
ficin D1
-
isoform
ficin D2
-
isoform
ficin E
-
-
ficin S
-
-
higueroxyl Delabarre
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9001-33-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Ficus anthelmintica
-
-
-
Manually annotated by BRENDA team
var. Horaishi
-
-
Manually annotated by BRENDA team
var. Kadota
-
-
Manually annotated by BRENDA team
ficin B ficin E, ficin F, ficin H
-
-
Manually annotated by BRENDA team
Ficus sp.
ficin II and ficin III
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-citrulline-p-nitroanilide + H2O
acetyl-citrulline + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
30% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
B chain of oxidized insulin + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Ala-Ala-OH
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Ala-Asp-OH
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Ala-Leu-OH
?
show the reaction diagram
-
weak activity
-
-
?
benzoyl-Arg-ethyl ester + Ala-NH2
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Ala-Pro-OH
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Arg-NH2
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Asp-NH2
?
show the reaction diagram
-
weak activity
-
-
?
benzoyl-Arg-ethyl ester + Gly-NH2
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-ethyl ester + Leu-NH2
?
show the reaction diagram
-
-
-
-
?
benzoyl-citrulline-p-nitroanilide + H2O
benzoyl-citrulline + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
81% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzoyl-D,L-arginine-p-nitroanilide + H2O
4-nitroaniline + ?
show the reaction diagram
-
-
-
-
?
benzoyl-D-Arg ethyl ester + H2O
benzoyl-D-Arg + ethanol
show the reaction diagram
-
-
-
-
?
benzoyl-DL-Arg-p-nitroanilide + H2O
benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
-
-
-
?
benzoyl-DL-Arg-p-nitroanilide hydrochloride + H2O
benzoyl-DL-Arg + 4-nitroaniline hydrochloride
show the reaction diagram
-
-
-
-
?
benzoyl-Gly-p-nitrophenyl ester + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
benzoyl-L-argininamide + H2O
benzoyl-L-Arg + NH3
show the reaction diagram
Ficus sp.
-
-
-
-
?
benzoyl-L-arginine 4-nitroanilide + H2O
benzoyl-L-arginine + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
-
-
-
?
benzoyl-Phe-Val-Arg-NH-(4-nitrophenyl) + H2O
benzoyl-Phe-Val-Arg + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin + H2O
benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala-methyl ester + Ala
benzyloxycarbonyl-Ala-Ala + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-methyl ester + Ala
benzyloxycarbonyl-Ala-D-Gln + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-methyl ester + Cys(acetamidomethyl)
benzyloxycarbonyl-Ala-Cys(acetamidomethyl) + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-citrulline-p-nitroanilide + H2O
benzyloxycarbonyl-citrulline + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
75% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-D-Ala-methyl ester + Ala
benzyloxycarbonyl-Ala-D-Ala + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gln-methyl ester + Ala
benzyloxycarbonyl-Ala-Gln + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
11% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Gly-Gly-Phe-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
activity is 2.97fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Gly-methyl ester + Ala
benzyloxycarbonyl-Gly-Ala + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-methyl ester + Cys(acetamidomethyl)
benzyloxycarbonyl-Gly-Cys(acetamidomethyl) + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-methyl ester + Gln
benzyloxycarbonyl-Gly-Gln + ?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-Phe-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
activity is 12.6fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Gly-Pro-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
39% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl) + H2O
benzyloxycarbonyl-Phe-Arg + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-NH-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
activity is 19.4fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Pro-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
23% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
hydrolysis ratio is 13% at 48 h
-
-
?
Collagen + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
Fibrin + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
Hemoglobin + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
hippuryl amide + H2O
hippuric acid + NH3
show the reaction diagram
Ficus sp.
-
-
-
-
?
hippuryl methyl ester + H2O
hippuric acid + methanol
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-acetyl-D-Phe-Gly 4-nitroanilide + H2O
N-acetyl-D-Phe-Gly + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
the index of stereochemical selectivity for N-acetyl-L-Phe-Gly 4-nitroanilide and N-acetyl-D-Phe-Gly 4-nitroanilide is 93
-
-
?
N-acetyl-L-Phe-Gly 4-nitroanilide + H2O
N-acetyl-L-Phe-Gly + 4-nitroaniline
show the reaction diagram
Ficus sp.
-
the index of stereochemical selectivity for N-acetyl-L-Phe-Gly 4-nitroanilide and N-acetyl-D-Phe-Gly 4-nitroanilide is 93
-
-
?
N-benzoyl-DL-arginine-2-naphthylamide + H2O
N-benzoyl-DL-arginine + 2-naphthylamine
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Ala + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Leu + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Phe + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Tyr + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
N-tert-butyloxycarbonyl-Ala-(1-4)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-(1-5)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-(1-6)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-(2-5)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-m-(guanidinomethyl)phenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-m-guanidinophenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-p-(guanidinomethyl)phenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-Ph + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + phenol
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-L-Ala-p-amidinophenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-L-Ala-L-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-L-Ala-p-guanidinophenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbony-L-Ala-L-Ala-p-nitroanilide + ?
show the reaction diagram
Ficus sp.
-
-
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
Nalpha-Benzoyl-L-Arg + ethanol
show the reaction diagram
Ficus sp.
-
-
-
-
?
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
Nalpha-Benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
-
-
?
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Ala + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
-
-
-
?
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester + H2O
?
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Leu p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Leu + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Lys + 4-nitrophenyl
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Phe + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Tyr + 4-nitrophenol
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Val p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Val + 4-nitrophenyl
show the reaction diagram
Ficus sp.
-
ficin II
-
-
?
p-nitrophenyl-carbobenzoxyglycinate + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
p-nitrophenyl-N-trans-acetyl-glycinate + H2O
?
show the reaction diagram
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl-N-trans-benzoyl-glycinate + H2O
?
show the reaction diagram
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl-N-trans-benzyloxycarbonyl-glycinate + H2O
?
show the reaction diagram
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl-N-trans-cinnamoyl-glycinate + H2O
?
show the reaction diagram
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl-N-trans-formyl-glycinate + H2O
?
show the reaction diagram
Ficus sp.
-
ficin II and III
-
-
?
Phe-citrulline-p-nitroanilide + H2O
?
show the reaction diagram
Ficus sp.
-
activity is 3.8fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
soy proteins + H2O
?
show the reaction diagram
Ficus sp.
-
-
-
-
?
tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide + H2O
tert-butyloxycarbonyl-L-Ala-L-Ala-Gly + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
insulin B chain + H2O
fragments of insulin B chain
show the reaction diagram
-
hydrolysis of 6 bonds
-
-
?
additional information
?
-
-
a wide variety of peptide bonds is hydrolyzed, but peptide bonds following an aromatic residue appear to be hydrolyzed more efficiently than the other
-
-
-
additional information
?
-
-
ficain is the major proteolytic activity component in the milky latex of the plant, substrate specificity compared to papain, the ficain S1-pocket accepts Gly, SEr, Glu, Tyr, and Phe, the S2-pocket accepts cysteic acid, Val, Leu, Gly, and Phe, Arg-NH-nitrophenyl is a poor substrate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
casein + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
ficain is the major proteolytic activity component in the milky latex of the plant
-
-
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
alpha2-Macroglobulin
Ficus sp.
-
-
-
amaranth cystatin
Ficus sp.
-
Amaranthus hypochondracus cysteine proteinase inhibitor, GenBank accession number DQ792503
-
Benzamidoacetonitrile
Ficus sp.
-
-
Chloroacetamide
Ficus sp.
-
-
Cystatin
-
from egg white, strong inhibition
-
Cystatin
Ficus sp.
-
from chicken egg
-
cysteine proteinase inhibitor in human seminal plasma
Ficus sp.
-
-
-
diisopropyl fluorophosphate
-
-
E-64
-
complete inhibition of all five ficin isoforms at 0.1 mM
HgCl2
Ficus sp.
-
-
inhibitors from rat and serum liver
Ficus sp.
-
-
-
iodoacetamide
-
activity is completely inhibited, cysteine-group specific inhibitor, confirms the participation of the cysteine residue at the active site of the enzyme
iodoacetamide
-
complete inhibition of all five ficin isoforms at 5 mM
iodoacetamide
Ficus sp.
-
-
iodoacetic acid
-
-
iodoacetic acid
Ficus sp.
-
-
Na2S4O6
-
-
-
Nalpha-tosyl-L-lysine chloromethyl ketone
-
complete inhibition of all five ficin isoforms at 0.1 mM
Nalpha-tosyl-L-phenylalanine chloromethylketone
-
complete inhibition of all five ficin isoforms at 0.1 mM
naturally occuring protein crystal in potato
Ficus sp.
-
-
-
NEM
Ficus sp.
-
-
p-chloromercuribenzoate
-
activity is completely inhibited by the cysteine-group specific inhibitor
phenylmethane sulfonyl fluoride
-
-
Proflavine
Ficus sp.
-
-
Proflavine
Ficus sp.
-
the inhibitor increases the noncovalent reversible binding affinity of ficin towards the maleimides without affecting the nucleophilic reactivity of the catalytically essential thiol groups which are alkylated in the inactivation reaction
Sodium tetrathionate
-
activity is completely inhibited by the cysteine-group specific inhibitor
methylmethanethiol sulfonate
-
complete inhibition of all five ficin isoforms at 3 mM
additional information
-
all five ficin isoforms are not inhibited by pepstatin A, Pafabloc SC, and EDTA
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Cys
-
activates
cysteine
-
the enzyme requires activation by reducing agents, optimal at 4 mM
EDTA
Ficus sp.
-
enhances activity
mercaptoethanol
-
activates
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3.3
-
benzoyl-DL-Arg-p-nitroanilide
Ficus sp.
-
-
0.0248
-
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 6.6
0.0272
-
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 5.61
0.0283
-
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 5.3
0.0513
-
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 3.91
0.189
-
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 3.15
0.0024
-
benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin
-
pH 6.8, 37C
0.2
-
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester
Ficus sp.
-
ficin II
0.024
-
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester
Ficus sp.
-
ficin II
0.128
-
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester
Ficus sp.
-
ficin II
0.012
-
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester
Ficus sp.
-
ficin II
21.3
-
Nalpha-benzoyl-L-Arg ethyl ester
Ficus sp.
-
-
0.0059
-
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
0.0122
-
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0258
-
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0125
-
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
-
0.0134
-
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
0.0598
-
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0172
-
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0087
-
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
0.052
-
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0377
-
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0034
-
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0327
-
Nalpha-carbobenzoxy-Val p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.309
-
p-nitrophenyl-N-trans-acetyl-glycinate
Ficus sp.
-
ficin III
-
0.328
-
p-nitrophenyl-N-trans-acetyl-glycinate
Ficus sp.
-
ficin II
-
0.0214
-
p-nitrophenyl-N-trans-benzoyl-glycinate
Ficus sp.
-
ficin III
-
0.025
-
p-nitrophenyl-N-trans-benzoyl-glycinate
Ficus sp.
-
ficin II
-
0.0183
-
p-nitrophenyl-N-trans-benzyloxycarbonyl-glycinate
Ficus sp.
-
ficin III
-
0.0194
-
p-nitrophenyl-N-trans-benzyloxycarbonyl-glycinate
Ficus sp.
-
ficin II
-
0.0067
-
p-nitrophenyl-N-trans-cinnamoyl-glycinate
Ficus sp.
-
ficin III
-
0.0076
-
p-nitrophenyl-N-trans-cinnamoyl-glycinate
Ficus sp.
-
ficin II
-
1.1
-
p-nitrophenyl-N-trans-formyl-glycinate
Ficus sp.
-
ficin III
-
0.3
-
benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl)
-
pH 6.8, 37C
additional information
-
additional information
-
little or no effect of temperature on Km
-
additional information
-
additional information
-
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0096
-
benzoyl-DL-Arg-p-nitroanilide
Ficus sp.
-
-
2.56
-
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 3.91
3.04
-
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 3.15
3.28
-
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 5.61 and at pH 6.6
3.41
-
benzoyl-Gly-p-nitrophenyl ester
Ficus sp.
-
pH 5.3
0.45
-
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester
Ficus sp.
-
ficin II
1.85
-
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester
Ficus sp.
-
ficin II
1.2
-
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester
Ficus sp.
-
ficin II
0.92
-
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester
Ficus sp.
-
ficin II
1.29
-
Nalpha-benzoyl-L-Arg ethyl ester
Ficus sp.
-
-
29.8
-
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
31
-
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
1.85
-
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
-
9.25
-
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
12.4
-
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
7.45
-
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
4.5
-
Nalpha-carbobenzoxy-Leu p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
35
-
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
55
-
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
2.83
-
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
2.27
-
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
1.02
-
Nalpha-carbobenzoxy-Val p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
2.3
-
p-nitrophenyl-N-trans-acetyl-glycinate
Ficus sp.
-
ficin II
-
2.84
-
p-nitrophenyl-N-trans-acetyl-glycinate
Ficus sp.
-
ficin III
-
7.72
-
p-nitrophenyl-N-trans-benzoyl-glycinate
Ficus sp.
-
ficin III
-
53.6
-
p-nitrophenyl-N-trans-benzoyl-glycinate
Ficus sp.
-
ficin II
-
13.3
-
p-nitrophenyl-N-trans-benzyloxycarbonyl-glycinate
Ficus sp.
-
ficin III
-
95.7
-
p-nitrophenyl-N-trans-benzyloxycarbonyl-glycinate
Ficus sp.
-
ficin III
-
86.4
-
p-nitrophenyl-N-trans-cinnamoyl-glycinate
Ficus sp.
-
ficin II
-
123
-
p-nitrophenyl-N-trans-cinnamoyl-glycinate
Ficus sp.
-
ficin III
-
2.5
-
p-nitrophenyl-N-trans-formyl-glycinate
Ficus sp.
-
ficin II
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.325
-
amaranth cystatin
Ficus sp.
-
Amaranthus hypochondracus cysteine proteinase inhibitor
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.044
-
-
purified isoform ficin A, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.046
-
-
purified isoform ficin B, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.073
-
-
purified isoform ficin C, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.11
-
-
purified isoform ficin D2, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.117
-
-
purified isoform ficin D1, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.127
-
-
purified isoform ficin A, using tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.146
-
-
purified isoform ficin D1, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.154
-
-
purified isoform ficin D2, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.186
-
-
purified isoform ficin C, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
0.204
-
-
purified isoform ficin B, using Nalpha-benzoyl-DL-arginine-4-nitroanilide as substrate, in sodium citrate, borate and phosphate (100 mM each) at pH 6.8 and 37C
6824
-
-
purifed enzyme against casein
additional information
-
Ficus sp.
-
-
additional information
-
-
-
additional information
-
Ficus sp.
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
Ficus sp.
-
-
7
-
-
in 50 mM sodium phosphate
9
-
Ficus sp.
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
9
-
pH 5.0: about 50% of maximal activity, pH 9.0: about 55% of maximal activity
6
11
-
pH 6.0: about 50% of maximal activity, pH 11.0: about 35% of maximal activity, hydrolysis of casein
6.5
8.5
-
maximum activity is observed between pH 6.5 and 8.5
additional information
-
-
pH profile
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
-
-
-
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
needs further purification
Manually annotated by BRENDA team
-
unripe, the enzyme is located in the latex milk secreted by the organ
Manually annotated by BRENDA team
-
the enzyme is located in the latex milk secreted by the organ
Manually annotated by BRENDA team
-
the enzyme is located in the latex milk secreted by the organ
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
10420
12030
-
multiple molecular forms, gel filtration
23100
-
-
determined by matrix-assisted laser desorption ionization-time of flight mass spectrometry
25200
-
-
ficin F, meniscus depletion method
25500
-
-
ficin E, meniscus depletion method
25500
-
-
equilibrium ultracentrifugation
25600
-
-
ficin H, meniscus depletion method
26100
-
-
ficin B, meniscus depletion method
26500
-
Ficus anthelmintica
-
ultracentrifugation
50000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * around 24000, native enzyme, SDS-PAGE
?
-
x * 26000, SDS-PAGE
monomer
-
enzyme is a single polypeptide chain protein
monomer
-
1 * 23100
additional information
-
amino acid sequence sand structure analysis
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
side-chain modification
-
sugar content 4.8%, sugar is tightly bound to enzyme protein
side-chain modification
-
carbohydrate content: 1.1% w/w for ficin B, less than 1% for ficin F1, ficin F2, and ficin H
side-chain modification
Ficus sp.
-
carbohydrate moiety comprises of glucosamine, mannose, galactose, fucose and xylose in molar ratios of 5:2:1:1:1; glycoprotein
additional information
-
the enzyme contains three disulfide bonds
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
active at neutral pH and looses its activity completely below pH 3.0
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
-
-
30 min, stable below
67
-
-
30 min, complete inactivation
70
-
-
ficin looses 37% of its activity at 70C after 10 min of incubation, and in the presence of co-solvents, the activity is protected up to 91%, maximum protection is shown by trehalose and followed by sorbitol, sucrose, and xylitol; ficin looses nearly 50% of its activity after 20 min of incubation; in the presence of sorbitol, the enzyme retained 82% of its activity after 20 min of incubation; in the presence of sucrose, the enzyme retained 80% of its activity after 20 min of incubation; in the presence of trehalose, the enzyme retained 85% of its activity after 20 min and 53% after 60 min of incubation; in the presence of xylitol, the enzyme retained 78% of its activity after 20 min of incubation
73
-
-
apparent denaturation temperature of ficin
85
-
-
the apparent denaturation temperature of ficin shifts in the presence of 40% trehalose
additional information
-
-
the apparent denaturation temperature of ficin in the presence of 10, 20, 30, and 40% sorbitol is 75, 77, 81 and 83C; the apparent denaturation temperature of ficin in the presence of 10, 20, 30, and 40% sucrose is 74, 77, 80 and 82C; the apparent denaturation temperature of ficin in the presence of 10, 20, 30, and 40% xylitol is 74, 76, 79 and 81C; the apparent thermal denaturation temperature of ficin is significantly increased from the control value of 72C in the presence of all co-solvents
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the maximum stabilization effect is observed in terms of thermal stabilization by the co-solvent trehalose
-
autodigestion during purification
-
9 M urea (1h): loss of about 65% of activity, 6 M guanidine hydrochloride (1h): complete loss of acivity
Ficus sp.
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
guanidine-HCl
-
ficin loses its complete structure in presence of 4 M guanidine-HCl at pH 7.0, ficin loses 50% activity at around 1 M guanidine-HCl, and is complete inactivated at 3 M
urea
-
the enzyme activity decreases significantly at low concentration of urea before unfolding of the enzyme molecule (about 50% residual activity at 2 M, about 20% residual activity at 4 M, no activity at 6 M)
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, pH 2.0-8.0, stable for 20 h
-
4C, 50% loss of activity after 4.5 months
-
4C, as salt precipitate, stable for at least 15 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
a major ficin from the commercial crude proteinase mixture preparation of Ficus carica is purified by using a SP-Sepharose cation-exchanger column on fast protein liquid chromatography
-
SP-Sepharose column chromatography
-
ficin II and III
-
further purification of a commercial latex preparation by cation exchange chromatography, and 2,2'-dipyridyl disulfide-glutathione or aminophenylmercuric acetate affinity chromatography, or by Gly-Phe-NH-methylcyanide affinity chromatography with elution by 2,2'-dipyridyl disulfide
-
multiple molecular forms
-
-
Ficus sp.
-
commercial preparation
Ficus sp.
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
industry
-
enzyme is used in the food, pharmaceutical, and detergent industries
synthesis
-
kinetically controlled formation of peptide bonds by coupling the ester substrates benzyloxycarbonyl-Ala methyl ester and benzyloxycarbonyl-Gly methyl ester with L-Ala, D-Ala, L-Gln, D-Gln and L-Cys(acetamidomethyl) respectively
analysis
Ficus sp.
-
the enzyme should prove generally useful in peptide sequencing
analysis
Ficus sp.
-
use for antibody screening of blood donor samples with the microplate system autoanalyzer
synthesis
Ficus sp.
-
digestion of murine monoclonal IgG to obtain immunoreactive bivalent antibody fragments