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Information on EC 3.4.22.28 - picornain 3C and Organism(s) Southampton virus and UniProt Accession Q04544

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.28 picornain 3C
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This record set is specific for:
Southampton virus
UNIPROT: Q04544 not found.
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Word Map
The taxonomic range for the selected organisms is: Southampton virus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Selective cleavage of Gln-/-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly
Synonyms
3c proteinase, protease 3c, 3c protein, human rhinovirus 3c protease, hrv 3c protease, rhinovirus 3c protease, 3c-protease, dhav 3c protease, ns6 protease, ev71 3cpro, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3C protease
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-
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3C proteinase
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-
-
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coxsackievirus 3C proteinase
-
-
-
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cysteine proteinase 3C
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-
-
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foot-and-mouth protease 3C
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-
-
-
HAC 3C
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-
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hepatitis A virus 3C proteinase
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-
-
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P3C
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-
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picornavirus endopeptidase 3C
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-
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poliovirus protease 3C
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poliovirus proteinase 3C
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protease 3C
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proteinase, foot-and-mouth-disease virus, 3C
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proteinase, picornavirus, 3C
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proteinase, poliovirus, 3C
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proteinase, rhinovirus, 3C
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rhinovirus protease 3C
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tomato ringspot nepovirus 3C-related protease
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
97162-88-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ac-DEFQLQ-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
Ac-EFQLQ-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
Ac-FQLQ-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-acetyl-L-alpha-glutamyl-L-phenylalanyl-L-glutaminyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-L-leucinamide
i.e. Michael acceptor peptidyl inhibitor (MAPI). Irreversible inhibitor in which the natural polyprotein substrate recognition sequence is linked to a propenyl ethyl ester moiety (X). The catalytic cysteine at position 139 of the enzyme is rapidly modified by the inhibitor
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
Ac-DEFQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
0.3
Ac-EFQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
1.5
Ac-FQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.33
Ac-DEFQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
0.14
Ac-EFQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
0.08
Ac-FQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.41
Ac-DEFQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
0.46
Ac-EFQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
0.05
Ac-FQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
X-ray structure of the Southampton norovirus 3C protease (SV3CP) bound to an active site-directed peptide inhibitor which has been refined at 1.7 A resolution is described
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hussey, R.J.; Coates, L.; Gill, R.S.; Erskine, P.T.; Coker, S.F.; Mitchell, E.; Cooper, J.B.; Wood, S.; Broadbridge, R.; Clarke, I.N.; Lambden, P.R.; Shoolingin-Jordan, P.M.
A Structural Study of Norovirus 3C Protease Specificity: Binding of a Designed Active Site-Directed Peptide Inhibitor
Biochemistry
50
240-249
2010
Southampton virus (Q04544)
Manually annotated by BRENDA team