Information on EC 3.4.22.26 - cancer procoagulant

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.22.26
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RECOMMENDED NAME
GeneOntology No.
cancer procoagulant
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
specific cleavage of Arg-/-Ile bond in Factor X to form Factor Xa
show the reaction diagram
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
109456-80-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
cancer procoagulant increases the expression of L1 cell adhesion molecule (L1CAM) mRNA and VEGF in MCF-7 breast cancer cells and E14 mouse embryonic stem cells; cancer procoagulant increases the steady-state mRNA level of angionin in MCF-7 breast cancer cells but decreases the expression of VEGF
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(D)-Leu-(L)-Ser-(L)-Thr-(L)-Arg p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
-
benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O
benzyloxycarbonyl-Arg-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Leu-Pro-Arg-4-nitroanilide + H2O
benzyloxycarbonyl-Leu-Pro-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Val-Pro-Arg-4-nitroanilide + H2O
benzyloxycarbonyl-Val-Pro-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
Bz-Ile-Glu-Arg-4-nitroanilide + H2O
Bz-Ile-Glu-Arg + 4-nitroaniline
show the reaction diagram
-
chromogenic commercial substrate
-
-
?
D-Ala-Pro-Arg-4-nitroanilide + H2O
D-Ala-Pro-Arg + 4-nitroaniline
show the reaction diagram
D-Ile-Pro-Arg-4-nitroanilide + H2O
D-Ile-Pro-Arg + 4-nitroaniline
show the reaction diagram
D-Phe-Pip-Arg-4-nitroanilide + H2O
D-Phe-Pip-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
factor X + H2O
?
show the reaction diagram
factor X + H2O
factor Xa
show the reaction diagram
Factor X + H2O
Factor Xa + ?
show the reaction diagram
Fibrinogen + H2O
?
show the reaction diagram
-
-
-
?
Glu-Glu-Phe-Lys-4-nitroanilide + H2O
Glu-Glu-Phe-Lys + 4-nitroaniline
show the reaction diagram
-
-
-
?
Glu-Glu-Pro-Arg-4-nitroanilide + H2O
Glu-Glu-Pro-Arg + 4-nitroaniline
show the reaction diagram
Leu-Pro-Arg-4-nitroanilide + H2O
Leu-Pro-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
methylsulfonyl-D-cyclohexylalanyl-butyl-Arg-4-nitroanilide + H2O
methylsulfonyl-D-cyclohexylalanyl-butyl-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
N-p-tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
N-p-tosyl-Gly-Pro-Arg + 4-nitroaniline
show the reaction diagram
N-p-tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
N-p-tosyl-Gly-Pro-Lys + 4-nitroaniline
show the reaction diagram
PAR-1 receptor + H2O
?
show the reaction diagram
Sar-Pro-Arg-p-nitroanilide + H2O
Sar-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
sarcosyl-Pro-Arg-4-nitroanilide + H2O
sarcosyl-Pro-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
thrombin TH + H2O
?
show the reaction diagram
-
-
-
-
-
Val-Pro-Arg-4-nitroanilide + H2O
Val-Pro-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
factor X + H2O
?
show the reaction diagram
factor X + H2O
factor Xa
show the reaction diagram
PAR-1 receptor + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
1 mM: increase of activity by factor 1.7
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(D)-Phe-Pro-(D,L)-(4'-guanidino)-Phe-trifluoromethyl ketone
-
-
(D)-Phe-Pro-(D,L)-Lys-trifluoromethyl ketone
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weak
(D)-Phe-Pro-Arg-chloromethyl ketone
-
-
2-Vinylpyridine
-
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alpha1-Antichymotrypsin
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alpha2-Macroglobulin
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antiCP polyclonal antibody
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-
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antiFVIIa polyclonal antibody
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-
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antipain
Benzoxycarbonyl-Lys-CHN2
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Benzoxycarbonyl-Phe-Ala-CHN2
-
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diisopropylfluorophosphate
E-64
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-
Hg2+
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0.1 mM: 85.5% decrease of activity
HgCl2
iodoacetamide
leupeptin
Mercuric chloride
Mn2+
-
above 1 mM, activates below 1 mM
p-chloromercuric benzoate
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Peptidyl chloromethanes
Peptidyl diazomethanes
Peptidyl diazomethyl ketones
Peptidyl dimethylsulfonium salts
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Peptidyl sulfonium salts
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-
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phenylmethylsulfonyl fluoride
trans-epoxysuccinyl-L-leucyl-amido(4-guanidino)butane
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.109
D-Ala-Pro-Arg-4-nitroanilide
-
pH 7.2
0.027
D-Ile-Pro-Arg-4-nitroanilide
-
pH 7.2
0.306
Glu-Glu-Pro-Arg-4-nitroanilide
-
pH 7.2
0.061
N-p-tosyl-Gly-Pro-Arg-4-nitroanilide
-
pH 7.2
0.153
N-p-tosyl-Gly-Pro-Lys-4-nitroanilide
-
pH 7.2
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0132
D-Ala-Pro-Arg-4-nitroanilide
Homo sapiens
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pH 7.2
0.0082
D-Ile-Pro-Arg-4-nitroanilide
Homo sapiens
-
pH 7.2
0.0119
Glu-Glu-Pro-Arg-4-nitroanilide
Homo sapiens
-
pH 7.2
0.0073
N-p-tosyl-Gly-Pro-Arg-4-nitroanilide
Homo sapiens
-
pH 7.2
0.0129
N-p-tosyl-Gly-Pro-Lys-4-nitroanilide
Homo sapiens
-
pH 7.2
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.128
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
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-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
Rattus norvegicus
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cancer procoagulant activity from 4 independent prostate tumors is assayed, inhibition of FX-activating activity of cancer procoagulant-containing extracts by use of a polyclonal antiFVIIa antibody, samples obtained from 1-step purification of crude extract are tested for the presence of tissue factor or FVIIa activity in inhibition assay using antirat factor VIIa polyclonal antibodies, 0% inhibition of cancer procoagulant sample 1, 0.4% inhibition of cancer procoagulant sample 2, 6.6% inhibition of cancer procoagulant sample 3, 4.9% inhibition of cancer procoagulant sample 4
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.059
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crude extract from independent PA3 tumor, activity of samples is determined by using a 3-stage chromogenic assay
0.068
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crude extract from independent PA3 prostate tumor, activity of samples is determined by using a 3-stage chromogenic assay
0.078
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crude extract from independent PA3 prostate tumor, activity of samples is determined by using a 3-stage chromogenic assay
0.082
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crude extract from independent PA3 rat prostate tumor, activity of samples is determined by using a 3-stage chromogenic assay
0.098
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crude extract from independent PA3 prostate tumor, activity of samples is determined by using a 3-stage chromogenic assay
2.26
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extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 33fold purification, activity of samples is determined by using a 3-stage chromogenic assay
2.32
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extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 30fold purification, activity of samples is determined by using a 3-stage chromogenic assay
4.91
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extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 50fold purification, activity of samples is determined by using a 3-stage chromogenic assay
7.7
-
extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 133fold purification, activity of samples is determined by using a 3-stage chromogenic assay
16.6
-
extract from independent PA3 prostate tumor undergoes 1 round of purification on the anion exchanger, 202fold purification, activity of samples is determined by using a 3-stage chromogenic assay
47
-
purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
-
assay at
additional information
-
pI: 4.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
breast cancer cell line
Manually annotated by BRENDA team
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amnion-chorion tissue
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
loosely anchored on outside of cells
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000
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mouse, Lewis lung tumor, gel filtration, procoagulant interacts with Sephacryl-200
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
-
peptide sequencing, isoelectric focusing, and peptide mapping
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no glycoprotein
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Polyethylene glycol preparation yields stable enzyme
Prefers lipid environment, phospholipids stabilize
Unstable hydrophobic enzyme which associates with other proteins or itself in tissue extracts and serum
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
sensitive to oxidation of active site, 1 mM 2-mercaptoethanol and 5 mM KCN or 0.005 mM cysteine and 4 mM KCN stabilize
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, inactivation within 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DE 52 cellulose chromatography
-
from Lewis lung tumor cells
-
harvested PA3 tumors are extracted in 0.02 M Tris (pH 7.8), after centrifugation extracts are purified by ion-exchange chromatography, fractions containing CP activity are desalted and concentrated by ultrafiltration on YM10 membrane and redissolved in 2-3 ml of 0.02 M Tris
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native enzyme from amniochorion membranes and from MCF-7 cells by ion exchange chromatographical steps to homogeneity
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native enzyme from amniochorion membranes by ion exchange chromatographical steps
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to homogeneity
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two different procedures
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine