Information on EC 3.4.22.24 - cathepsin T

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY hide
3.4.22.24
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RECOMMENDED NAME
GeneOntology No.
cathepsin T
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
interconversion of the three forms of tyrosine aminotransferase, EC 2.6.1.5
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
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CAS REGISTRY NUMBER
COMMENTARY hide
77464-86-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Acid-denatured hemoglobin + H2O
?
show the reaction diagram
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-
-
-
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azocasein + H2O
?
show the reaction diagram
Benzoxycarbonyl-Phe-Arg-methylcoumarylamide + H2O
?
show the reaction diagram
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-
-
-
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Hemoglobin + H2O
?
show the reaction diagram
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denaturation
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-
?
Performic acid-oxidized ribonuclease A + H2O
Hydrolyzed performic acid-oxidized ribonuclease A
show the reaction diagram
Tyrosine aminotransferase + H2O
?
show the reaction diagram
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involved in generation of isozymic forms of tyrosine transferase
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tyrosine aminotransferase + H2O
truncated tyrosine aminotransferase + peptide
show the reaction diagram
Tyrosine aminotransferase form I + H2O
Tyrosine aminotransferase form II and III + acidic peptide of MW 4500 peptide
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Tyrosine aminotransferase + H2O
?
show the reaction diagram
-
involved in generation of isozymic forms of tyrosine transferase
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-
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tyrosine aminotransferase + H2O
truncated tyrosine aminotransferase + peptide
show the reaction diagram
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53 kDa substrate monomer, cleavage at the N-terminus of the protein, interconversion of the 3 different forms of the substrate enzyme without alteration of its enzymatic activity related to the substrate's short half-life in the cell
49 kDa product monomer and 4.5 kDa N-terminal peptide
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?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4,4'-dipyridyl disulfide
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iodoacetamide
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iodoacetate
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Low molecular weight thiol proteinase inhibitor fractions
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of human or rat serum, human synovial fluid and cerebrospinal fluid, MW 17000
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N-ethylmaleimide
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Oxidized ribonuclease A
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tyrosine aminotransferase as substrate
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tosyl-L-lysine chloromethyl ketone
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tosyl-Lys-methylchloride
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-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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activation, 4 mM
cysteine
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activation, 4 mM
GSH
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activation, 4 mM
sulfhydryl compounds
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activation
additional information
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carbon tetrachloride induces the enzyme 7fold in the liver
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
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active in this range, negligible below pH 5 and above pH 8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
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assay at, tyrosine aminotransferase as substrate
37
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assay at, proteins as substrate
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
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tissue distribution, no activity in heart, skeletal muscle, brain and blood
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
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15 min, 37C, 50% loss of activity
30140
7
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above, 0C, 1.25% glycerol, rapid loss of activity
30140
7.8
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20 min, 0C, inactivation
30140
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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15 min, pH 6.5, 50% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70C, in 50 mM sodium phosphate buffer, pH 6.5, 25 mM NaCl, 12.5% glycerol, 1 mM DTT, at least 2 months
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4C, at somewhat higher ionic strength than 50 mM sodium phosphate, pH 6.5, 25 mM NaCl, 12.5% glycerol, 1 mM DTT, inactivation within 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
kidney; liver
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native enzyme to homogeneity from liver
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