Information on EC 3.4.21.B59 - granzyme H

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The expected taxonomic range for this enzyme is: Homo sapiens

EC NUMBER
COMMENTARY hide
3.4.21.B59
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
granzyme H
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites
show the reaction diagram
-
-
-
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preferential cleavage: -Asp-/- >> -Asn-/- > -Met-/-, -Ser-/-
show the reaction diagram
-
-
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme induces cell death primarily via a Bcl-2-sensitive mitochondrial cell death pathway that does not require direct Bid activation, overview. Neither the apoptosome nor caspase-3 is essential to the induction of GrH-mediated cell death. The enzyme might have pro-apoptotic activity that might be distinct from that of granzyme B and FasL, which can be relevant when Fas/FasL or granzyme B activity or death pathways are impaired. Direct processing of DFF45 by the enzyme may be leading to DNA damage. The enzyme inhibits caspase-3 activation
additional information
Bid is not required for enzyme-mediated cell death
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DFF45 protein + H2O
?
show the reaction diagram
additional information
?
-
granzyme H does not efficiently process Bid
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DFF45 protein + H2O
?
show the reaction diagram
P20718
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
granzyme H is a serine protease, initially produced as a zymogen that requires the activity of granule-resident cathepsin C to become active. Cell death in cytotoxic potential of the enzyme with replication-deficient adenovirus 5 in target cells, i.e. K562 cells, Jurkat cells, and EBV-transformed B cell line RPMI-8866,the enzyme is less effective against tumor cells. Enzyme GrH induces cytochrome C loss from the mitochondrial intermembrane space. Similar to cytochrome C, SMAC/DIABLO is also observed to undergo translocation from the mitochondria to the cytosol after treatment with the enzyme
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
isolation of enzymatically active recombinant wild-type enzyme and of inactive mutant S202A from RBL-2H3 granules
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant expression of enzymatically active enzyme in RBL-2H3 granules
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S202A
inactive mutant