Information on EC 3.4.21.B58 - pernilase

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The expected taxonomic range for this enzyme is: Aeropyrum pernix

EC NUMBER
COMMENTARY hide
3.4.21.B58
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
pernilase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
azocasein + H2O
?
show the reaction diagram
benzoyl-Arg-amido-4-methyl-coumarin + H2O
benzoyl-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Boc-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Boc-Leu-Gly-Arg-amido-4-methyl-coumarin + H2O
Boc-Leu-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
Boc-Phe-Ser-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
1 mM, 34% inhibition
diisopropyl fluorophosphate
-
1 mM, 88% inhibition
EDTA
-
1 mM, 30% inhibition
EGTA
-
1 mM, 40% inhibition
PMSF
-
1 mM, 87% inhibition
Soybean trypsin inhibitor
-
1 mg/ml 40% inhibition
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80 - 110
-
80°C: about 65% of maximal activity, 110°C: about 75% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
half-life: 85 min
110
-
half-life: 12 min
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-mercaptoethanol
dithiothreitol
guanidine-HCl
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
highly resistant to the reducing agents dithiothreitol and 2-mercaptoethanol
-
727450
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE