Information on EC 3.4.21.B54 - Pyrococcus abyssi serine endopeptidase

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The expected taxonomic range for this enzyme is: Pyrococcus abyssi

EC NUMBER
COMMENTARY hide
3.4.21.B54
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
Pyrococcus abyssi serine endopeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
high affinity for aromatic (Phe and Tyr) and hydrophobic amino acids (mainly Leu) in P1 position, specificity for aromatic moieties in P1' position
show the reaction diagram
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-
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
oxidized insulin B-chain + H2O
oxidized insulin B-chain peptide fragments
show the reaction diagram
succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline
show the reaction diagram
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-(2-aminoethyl)-benzenesulfonyl fluoride
-
90% inhibition
Ca2+
-
0.01-0.1 M: 50-60% decrease in activity
KCl
-
0.2-0.45 M: no effect. 0.1 M: 30-40% decrease in activity
Mg2+
-
0.01-0.1 M: 50-60% decrease in activity
NaCl
-
0.2-0.45 M: no effect. 0.1 M: 30-40% decrease in activity
phenylmethylsulfonyl fluoride
-
80% ihibition
Soybean trypsin inhibitor
-
100% inhibition
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 11
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pH 7.0: about 40% of maximal activity, pH 11.0: about 40% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60 - 95
-
60°C: about 40% of maximal activity, 95°C: maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18800
-
x * 18800, PfPI protease has a monomeric form of 18800 Da oligomerising into a form of 200000 Da. The monomer and dimer are proteolyticaly inactive, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oligomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
t1/2: 165 min
105
-
t1/2: 29 min
110
-
t1/2: 7.3 min
115
-
t1/2: 2.2 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the enzyme is inactivated instantly in the presence of SDS
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE