Information on EC 3.4.21.B52 - thermostable serine endopeptidase (Sulfolobus)

Word Map on EC 3.4.21.B52
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Sulfolobus solfataricus

EC NUMBER
COMMENTARY hide
3.4.21.B52
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
thermostable serine endopeptidase (Sulfolobus)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
The best substrate is benzyloxycarbonyl-Tyr-4-nitroanilide. Cleaves Phe-Ser-Lys-Phe-Ile-/-Ser-Phe-Asp. No activity benzyloxycarbonyl-Ile-4-nitroanilide, aminoacyl-4-nitroanilides or benzyloxycarbonyl-amino acids, oxidized insulin chain A and B, bovine pancreas ribonuclease A, bovine serum albumin or ovalbumin.
show the reaction diagram
-
-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzyloxycarbonyl-Ala-Ala-Leu-4-nitroanilide + H2O
benzyloxycarbonyl-Ala-Ala-Leu + 4-nitroaniline
show the reaction diagram
-
1% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide
-
-
?
benzyloxycarbonyl-Leu-4-nitroanilide + H2O
benzyloxycarbonyl-Leu + 4-nitroaniline
show the reaction diagram
-
36% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide
-
-
?
benzyloxycarbonyl-Tyr-4-nitroanilide + H2O
benzyloxycarbonyl-Tyr + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Phe-Ser-Lys-Phe-Ile-Ser-Phe-Asp + H2O
Phe-Ser-Lys-Phe-Ile + Ser-Phe-Asp
show the reaction diagram
-
no cleavage between Phe4 and Ile5
-
-
?
Suc-Ala-Ala-Phe-4-nitroanilide + H2O
Suc-Ala-Ala-Phe + 4-nitroaniline
show the reaction diagram
-
3% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide
-
-
?
Suc-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
Suc-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
-
2% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide
-
-
?
Suc-Gly-Gly-Phe-4-nitroanilide + H2O
Suc-Gly-Gly-Phe + 4-nitroaniline
show the reaction diagram
-
6.5% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide
-
-
?
Suc-Phe-4-nitroanilide + H2O
Suc-Phe + 4-nitroaniline
show the reaction diagram
additional information
?
-
-
narrow substrate specificity. No activity with: aminoacyl-4-nitroanilides or benzyloxycarbonyl-amino acids, oxidized insulin chain A and B, bovine pancreas ribonuclease A, bovine serum albumin, ovalbumin. No activity with benzyloxycarbonyl-Ile-4-nitroanilide
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
activation effect up to 2 mM
Mg2+
-
no effect by Mg2+, Ca2+ and Zn2+ over the concentration range 0.2-20 mM
Mn2+
-
significantly stimulates, mixed type non-essential activator
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
chymostatin
iodoacetate
-
1 mM, 10% inhibition
N-alpha-p-tosyl-L-lysine chloromethyl ketone
-
0.3 mM, 6% inhibition
N-alpha-p-tosyl-L-phenylalanine chloromethyl ketone
-
0.5 mM, complete inhibition
p-chloromercuribenzenesulfonate
-
10 mM, 13% inhibition
p-chloromercuribenzoate
-
0.6 mM, 6% inhibition
phenylmethanesulfonyl fluoride
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.34
benzyloxycarbonyl-Leu-4-nitroanilide
-
pH 7.0, 80C
0.11
benzyloxycarbonyl-Tyr-4-nitroanilide
-
pH 7.0, 80C
1.16
Suc-Ala-Ala-Phe-4-nitroanilide
-
pH 7.0, 80C
0.85
Suc-Ala-Ala-Pro-Phe 4-nitroanilide
-
pH 7.0, 80C
0.72
Suc-Gly-Gly-Phe-4-nitroanilide
-
pH 7.0, 80C
1.34
Suc-Phe-4-nitroanilide
-
pH 7.0, 80C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12.1
benzyloxycarbonyl-Leu-4-nitroanilide
Sulfolobus solfataricus
-
pH 7.0, 80C
23
benzyloxycarbonyl-Tyr-4-nitroanilide
Sulfolobus solfataricus
-
pH 7.0, 80C
2.2
Suc-Ala-Ala-Phe-4-nitroanilide
Sulfolobus solfataricus
-
pH 7.0, 80C
1
Suc-Ala-Ala-Pro-Phe 4-nitroanilide
Sulfolobus solfataricus
-
pH 7.0, 80C
3.3
Suc-Gly-Gly-Phe-4-nitroanilide
Sulfolobus solfataricus
-
pH 7.0, 80C
15.6
Suc-Phe-4-nitroanilide
Sulfolobus solfataricus
-
pH 7.0, 80C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
35
benzyloxycarbonyl-Leu-4-nitroanilide
Sulfolobus solfataricus
-
pH 7.0, 80C
42219
210
benzyloxycarbonyl-Tyr-4-nitroanilide
Sulfolobus solfataricus
-
pH 7.0, 80C
42214
1.9
Suc-Ala-Ala-Phe-4-nitroanilide
Sulfolobus solfataricus
-
pH 7.0, 80C
42216
1.2
Suc-Ala-Ala-Pro-Phe 4-nitroanilide
Sulfolobus solfataricus
-
pH 7.0, 80C
42217
4.6
Suc-Gly-Gly-Phe-4-nitroanilide
Sulfolobus solfataricus
-
pH 7.0, 80C
42218
10.7
Suc-Phe-4-nitroanilide
Sulfolobus solfataricus
-
pH 7.0, 80C
42215
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28.9
-
pH 7.0, 80C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
in absence of divalent cations
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
-
isoelectric focusing
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54000
-
2 * 54000, SDS-PAGE
115000
-
gel filtration
118000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 54000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
enzyme can withstand 6 M urea
92
-
first-order thermal inactivation, half-life: 342 min
101
-
first-order thermal inactivation, half-life: 7 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the enzyme is stable for 60 min in buffer containing 1 M NaCl at 50C, it loses 96% of its activity after 60 min in 1 mM NaCl at 95C
-
the enzyme loses 54% of its activity after 60 min in buffer containing 2 mM dithiothreitol at 95C
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetonitrile
-
50% acetonitrile, 91% inhibition after 30 min at 50C
Ethanol
-
enzyme can withstand 50% ethanol at 50C, 98% loss of activity after 30 min at 95C. 45% loss of activity after 30 min, in 99% ethanol
Glycerol
-
20% glycerol, 35% inhibition after 30 min at 95C
Methanol
-
50% methanol, 66% loss of activity after 30 min at 50C, 98% loss of activity after 30 min, at 95C
tetrahydrofuran
-
50% tetrahydrofuran, 77% inhibition after 30 min at 50C, 96% inhibition after 30 min at 95C
urea
-
enzyme can withstand 6 M urea at 50C, 97% loss of activity after 30 min at 95C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE