Information on EC 3.4.21.B5 - mast cell protease 5

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.21.B5
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
mast cell protease 5
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endoprotease activity
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cleavage of C-N-linkage
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
142539-77-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Protein + H2O
?
show the reaction diagram
succinyl-Ala-Ala-Pro-Ala-4-nitroanilide + H2O
?
show the reaction diagram
highest activity
-
?
succinyl-Ala-Ala-Pro-Ile-4-nitroanilide + H2O
?
show the reaction diagram
-
-
?
succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O
?
show the reaction diagram
-
-
?
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
?
show the reaction diagram
low activity
-
?
succinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
?
show the reaction diagram
highest activity
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Protein + H2O
?
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-1-antitrypsin
forms a covalent complex with the enzyme, heparin protects complex formation
-
diisopropyl fluorophosphates
binds covalently to the enzyme
additional information
-
the CB1 and CB2 receptor agonists arachidonyl-20-chloroethylamide and 2-methyl-(1-propyl-1H-indol-3-yl)-1-naphthalenylmethanone (0.001-0.1 mg/ml) prevent the transcription and expression of mast cell protease-5
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.6
succinyl-Ala-Ala-Pro-Ala-4-nitroanilide
pH 8.5
3.1
succinyl-Ala-Ala-Pro-Ile-4-nitroanilide
pH 8.5
4
succinyl-Ala-Ala-Pro-Val-4-nitroanilide
pH 8.5
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.029
succinyl-Ala-Ala-Pro-Ala-4-nitroanilide
Rattus norvegicus
P50339
pH 8.5
0.0072
succinyl-Ala-Ala-Pro-Ile-4-nitroanilide
Rattus norvegicus
P50339
pH 8.5
0.017
succinyl-Ala-Ala-Pro-Val-4-nitroanilide
Rattus norvegicus
P50339
pH 8.5
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.4
-
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000 - 26000
-
SDS-PAGE
25270
-
calculated from amino acid sequence
25390
calculated from amino acid sequence
25400
calculated from amino acid sequence
30000
-
SDS-PAGE, MMCP-5'in serosal mast cells
31000
-
SDS-PAGE, MMCP-5 in serosal mast cells and Kirsten sarcoma virus-immortalized mast cells
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
allelic variant MMCP-5.1; allelic variant MMCP-5.4
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
1% (w/v) lambda-carrageenan treatment results in accumulation of mast cell protease-5 transcript compared to saline
-
the administration of palmitoylethanolamide (200, 400, and 800 mg/ml) significantly decreases mast cell protease-5 mRNA expression
-