Information on EC 3.4.21.B49 - ctpB peptidase

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The expected taxonomic range for this enzyme is: Bacillus subtilis

EC NUMBER
COMMENTARY hide
3.4.21.B49
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
ctpB peptidase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
involved in sigmaK signaling, acts in cooperation with SpoIVB
show the reaction diagram
-
-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
BofA + H2O
fragments of BofA
show the reaction diagram
-
involved in sigmaK signaling, acts in cooperation with SpoIVB, substrate and enzyme coexpressed in Escherichia coli, cleavage site near the C-terminus
-
-
?
GFP-delta27 BofA + H2O
?
show the reaction diagram
-
-
one or two low molecular weight species detected
-
?
His tagged extracellular domain of SpoIVFA + H2O
?
show the reaction diagram
-
two cleavage sites identified, cleavage sites different from those of SpoIVB
-
-
?
SpoIVFA + H2O
fragements of SpoIVFA
show the reaction diagram
-
enzyme can substitute the function of SpoIVB, involved in sigmaK processing
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
BofA + H2O
fragments of BofA
show the reaction diagram
-
involved in sigmaK signaling, acts in cooperation with SpoIVB, substrate and enzyme coexpressed in Escherichia coli, cleavage site near the C-terminus
-
-
?
SpoIVFA + H2O
fragements of SpoIVFA
show the reaction diagram
-
enzyme can substitute the function of SpoIVB, involved in sigmaK processing
-
-
?
additional information
?
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein using His-tag
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as His-tag fusion protein
-
expressed as His-tag fusion protein in Escherichia coli
-
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) pLys
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A36R
-
not cleavable by SpoIVB
A40R
-
not cleavable by SpoIVB
A36R
-
not cleavable by SpoIVB
-
A40R
-
not cleavable by SpoIVB
-
S309A
-
inactive; missense mutation; unable to cleave SpoIVFA
-
additional information