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Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Ala-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Arg-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Arg-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Azocoll + H2O
?
-
-
-
-
?
Benzyloxycarbonyl-L-Ala-L-Ala-L-Leu 4-nitroanilide + H2O
?
bovine alpha-globulin + H2O
?
-
-
-
-
?
Bovine insulin B-chain + H2O
?
-
-
-
-
?
Collagen + H2O
?
-
-
-
-
?
haemoglobin + H2O
?
-
-
-
-
?
high-powder azure + H2O
?
-
-
-
-
?
Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Leu-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Lys-Ala-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Ala-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Arg-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Arg-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Asp-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Leu-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Leu-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Ala-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Arg-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Asp-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ala + H2O
Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Ala
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg + H2O
Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Arg
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Asp + H2O
Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Asp
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ser + H2O
Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Ser
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Ser-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Arg-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Arg-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Arg-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Asn-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Asp-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Asp-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Gln-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Gln-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Glu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Glu-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu + H2O
Lys-Pro-Ile-Ala-Phe + Nph-Arg-Leu
Lys-Pro-Ile-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Ala-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Arg-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Asn-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Asp-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Gln-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ala-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Ala-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ala + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Ala
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Arg + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Arg
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Asp + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Asp
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ile + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Ile
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Nle + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Nle
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Phe
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ser + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Ser
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Tyr + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Tyr
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Val + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Val
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Asn-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Asp-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Glu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Glu-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Ile-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Leu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Leu-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Lys-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Lys-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ser-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Ser-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Val-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-His-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-His-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Ile-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Ile-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Leu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Nle-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Nle-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Pro-Phe-(4-nitrophenyl)-Arg-Leu + H2O
Lys-Pro-Ile-Pro-Phe + (4-nitrophenyl)-Arg-Leu
Lys-Pro-Ile-Pro-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Pro-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Ser-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Thr-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Val-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Leu-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Lys-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Lys-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Phe-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ser-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ser-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ser-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Thr-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Thr-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Val-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Val-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Ser-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Ser-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Oxidized insulin B-chain + H2O
?
Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Ser-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide + H2O
[2-(N-methylamino)benzoyl]-MGPH + FFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide
additional information
?
-
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Ala-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Ala-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
-
-
-
?
Benzyloxycarbonyl-L-Ala-L-Ala-L-Leu 4-nitroanilide + H2O
?
-
-
-
-
?
Benzyloxycarbonyl-L-Ala-L-Ala-L-Leu 4-nitroanilide + H2O
?
-
-
-
-
?
casein + H2O
?
Bacillus novosp.
-
-
-
?
IQF peptide + H2O
?
-
IQF fluorogenic peptide substrates
-
-
?
IQF peptide + H2O
?
-
IQF fluorogenic peptide substrates
-
-
?
Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Leu-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Leu-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
-
-
-
?
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu + H2O
Lys-Pro-Ile-Ala-Phe + Nph-Arg-Leu
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu + H2O
Lys-Pro-Ile-Ala-Phe + Nph-Arg-Leu
-
-
-
?
Lys-Pro-Ile-Pro-Phe-(4-nitrophenyl)-Arg-Leu + H2O
Lys-Pro-Ile-Pro-Phe + (4-nitrophenyl)-Arg-Leu
-
-
-
-
?
Lys-Pro-Ile-Pro-Phe-(4-nitrophenyl)-Arg-Leu + H2O
Lys-Pro-Ile-Pro-Phe + (4-nitrophenyl)-Arg-Leu
-
-
-
-
?
Oxidized insulin B-chain + H2O
?
Bacillus novosp.
-
specifically hydrolyzes Leu15-Tyr16 peptide bond in oxidized insulin B-chain, additional cleavage at Phe25-Tyr26 at a considerably lower rate
-
?
Oxidized insulin B-chain + H2O
?
-
specifically hydrolyzes Leu15-Tyr16 peptide bond in oxidized insulin B-chain, additional cleavage at Phe25-Tyr26 at a considerably lower rate
-
?
Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Ser-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
-
?
Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Ser-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
-
-
-
?
[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide + H2O
[2-(N-methylamino)benzoyl]-MGPH + FFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide
-
-
-
?
[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide + H2O
[2-(N-methylamino)benzoyl]-MGPH + FFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide
-
-
-
?
additional information
?
-
Bacillus novosp.
-
the enzyme preferentially hydrolyzes peptides having an Ala or Pro residue at P2 position and prefers such charged amino acid residues as Glu or Arg at the P2' position. No cleavage: Asp-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
-
?
additional information
?
-
-
the enzyme preferentially hydrolyzes peptides having an Ala or Pro residue at P2 position and prefers such charged amino acid residues as Glu or Arg at the P2' position. No cleavage: Asp-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
-
?
additional information
?
-
-
substrate specificity, overview, the enzyme prefers charged amino acid residues at P2' position
-
-
?
additional information
?
-
-
substrate specificity, overview, the enzyme prefers charged amino acid residues at P2' position
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0382
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.103
Arg-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0007 - 0.001
IQF peptide
-
0.0269
Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0517
Lys-Ala-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0128
Lys-Arg-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0349
Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0428
Lys-Leu-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0636
Lys-Pro-Ala-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0154
Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.133
Lys-Pro-Ala-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0406
Lys-Pro-Ala-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0567
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ala
Bacillus novosp.
-
-
0.0122
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg
Bacillus novosp.
-
-
0.0674
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Asp
Bacillus novosp.
-
-
0.056
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0527
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ser
Bacillus novosp.
-
-
0.0645
Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.119
Lys-Pro-Arg-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.142
Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0377
Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0654
Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0351
Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0376
Lys-Pro-Gln-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0404
Lys-Pro-Glu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.029 - 0.102
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
0.0426
Lys-Pro-Ile-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0767
Lys-Pro-Ile-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.073
Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0405
Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0377
Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0371
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ala-Leu
Bacillus novosp.
-
-
0.0995
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ala
Bacillus novosp.
-
-
0.154
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Arg
Bacillus novosp.
-
-
0.109
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Asp
Bacillus novosp.
-
-
0.0697
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ile
Bacillus novosp.
-
-
0.0603
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.119
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Nle
Bacillus novosp.
-
-
0.0634
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe
Bacillus novosp.
-
-
0.129
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ser
Bacillus novosp.
-
-
0.0248
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Tyr
Bacillus novosp.
-
-
0.0589
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Val
Bacillus novosp.
-
-
0.43
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu
Bacillus novosp.
-
-
0.225
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu
Bacillus novosp.
-
-
0.0438
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Glu-Leu
Bacillus novosp.
-
-
0.0285
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu
Bacillus novosp.
-
-
0.109
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Leu-Leu
Bacillus novosp.
-
-
0.128
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Lys-Leu
Bacillus novosp.
-
-
0.0473
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ser-Leu
Bacillus novosp.
-
-
0.0883
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu
Bacillus novosp.
-
-
0.0831
Lys-Pro-Ile-His-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0128
Lys-Pro-Ile-Ile-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.041
Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0671
Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0468
Lys-Pro-Ile-Nle-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.041
Lys-Pro-Ile-Pro-Phe-(4-nitrophenyl)-Arg-Leu
-
pH 3.5, 60°C
0.0409
Lys-Pro-Ile-Pro-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0642
Lys-Pro-Ile-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0447
Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0825
Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0575
Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0246
Lys-Pro-Lys-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0389
Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.1
Lys-Pro-Ser-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0577
Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0469
Lys-Pro-Thr-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.105
Lys-Pro-Val-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.162
Lys-Ser-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.09
oxidized insulin B-chain
Bacillus novosp.
-
pH 3.0, 30°C
-
0.0211
Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0007
[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide
-
mutant enzyme E78H
additional information
additional information
-
0.0007
IQF peptide
-
pH 4.0, 40°C, recombinant mutant E78H
-
0.0008
IQF peptide
-
pH 4.0, 40°C, recombinant mutant D164N
-
0.001
IQF peptide
-
pH 4.0, 40°C, recombinant wild-type enzyme
-
0.029
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
Bacillus novosp.
-
pH 3.5, 60°C, wild-type enzyme
0.084
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
Bacillus novosp.
-
pH 3.5, 60°C, mutant enzyme S278A
0.089
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
Bacillus novosp.
-
pH 3.5, 60°C, mutant enzyme W129A
0.102
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
Bacillus novosp.
-
pH 3.5, 60°C, mutant enzyme E32A
additional information
additional information
-
kinetics, wild-type and mutant enzymes, and pH-dependency
-
additional information
additional information
Bacillus novosp.
-
thermodynamic analysis
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.54 - 6.08
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
0.00045 - 395
IQF peptide
-
0.26
Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.5
Lys-Ala-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.08
Lys-Arg-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
1.46 - 6.08
Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
20.3
Lys-Leu-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
98.3
Lys-Pro-Ala-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.98 - 6.08
Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu
0.22
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ala
Bacillus novosp.
-
-
1.04 - 6.08
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg
0.19
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Asp
Bacillus novosp.
-
-
0.41
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.45
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ser
Bacillus novosp.
-
-
3.5 - 19.9
Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu
32.3
Lys-Pro-Arg-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
1.13 - 6.08
Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
3.3 - 4.62
Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
3 - 6
Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
0.64 - 6.08
Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
16.1
Lys-Pro-Gln-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
13.1
Lys-Pro-Glu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.08 - 6.08
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
48.5
Lys-Pro-Ile-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.04 - 9.14
Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu
28.6 - 81
Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu
3.3 - 4.79
Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu
7.7
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ala-Leu
Bacillus novosp.
-
-
21.7
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Asp
Bacillus novosp.
-
-
12.5
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ile
Bacillus novosp.
-
-
25.4
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
34.2
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Nle
Bacillus novosp.
-
-
3.7 - 31.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe
15.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Tyr
Bacillus novosp.
-
-
13.1
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Val
Bacillus novosp.
-
-
3 - 6
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu
0.04 - 8.78
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu
22.7
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Glu-Leu
Bacillus novosp.
-
-
1.82 - 2.94
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu
23.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Leu-Leu
Bacillus novosp.
-
-
10.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Lys-Leu
Bacillus novosp.
-
-
2.6
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ser-Leu
Bacillus novosp.
-
-
3.3 - 5.04
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu
3
Lys-Pro-Ile-His-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.3
Lys-Pro-Ile-Ile-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.84 - 6.08
Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu
176
Lys-Pro-Ile-Pro-Phe-(4-nitrophenyl)-Arg-Leu
-
pH 3.5, 60°C
176
Lys-Pro-Ile-Pro-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
52.7
Lys-Pro-Ile-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3 - 6
Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu
0.73 - 6.08
Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu
3.13 - 4.44
Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
3.13 - 3.76
Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
0.052 - 2.1
Lys-Pro-Ser-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.59 - 6.08
Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
16.7
Lys-Pro-Thr-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
18.6
Lys-Pro-Val-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
2.35
Lys-Ser-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
71
oxidized insulin B-chain
Bacillus novosp.
-
pH 3.0, 30°C
-
0.27
Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.033
[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide
-
mutant enzyme E78H
additional information
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
0.54
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.08
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.00045
IQF peptide
-
pH 4.0, 40°C, recombinant mutant E78H/D164N
-
0.033
IQF peptide
-
pH 4.0, 40°C, recombinant mutant E78H
-
5.3
IQF peptide
-
pH 4.0, 40°C, recombinant mutant D164N
-
395
IQF peptide
-
pH 4.0, 40°C, recombinant wild-type enzyme
-
1.46
Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.08
Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.98
Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.08
Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
1.04
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg
Bacillus novosp.
-
-
6.08
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg
Bacillus novosp.
-
-
3.5
Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
19.9
Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
1.13
Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.08
Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3.3
Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
4.62
Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3 - 6
Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3.12
Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.64
Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.08
Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.08
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
Bacillus novosp.
-
pH 3.5, 60°C, mutant enzyme S278A
0.67
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
Bacillus novosp.
-
pH 3.5, 60°C, mutant enzyme W129A
1.24
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
Bacillus novosp.
-
pH 3.5, 60°C, mutant enzyme E32A
6.02
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
Bacillus novosp.
-
pH 3.5, 60°C, wild-type enzyme
6.08
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
Bacillus novosp.
-
pH 3.5, 60°C, mutant enzyme E32A
6.08
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
Bacillus novosp.
-
pH 3.5, 60°C, mutant enzyme W129A
0.04 - 1.97
Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
9.14
Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
28.6
Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
81
Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3.3
Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
4.79
Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3.7
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe
Bacillus novosp.
-
-
31.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe
Bacillus novosp.
-
-
3 - 6
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu
Bacillus novosp.
-
-
3.48
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu
Bacillus novosp.
-
-
0.04 - 1.97
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu
Bacillus novosp.
-
-
8.78
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu
Bacillus novosp.
-
-
1.82
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu
Bacillus novosp.
-
-
2.94
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu
Bacillus novosp.
-
-
3.3
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu
Bacillus novosp.
-
-
5.04
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu
Bacillus novosp.
-
-
0.84
Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.08
Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3 - 6
Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3.48
Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.73
Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.08
Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3.13
Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
4.44
Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3.13
Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3.76
Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.59
Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.08
Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
additional information
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
Bacillus novosp.
-
pH 3.5, 60°C, wild-type enzyme
additional information
additional information
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
19000
-
x * 38000, active recombinant enzyme, SDS-PAGE, x * 19000, recombinant isolated propeptide, SDS-PAGE, x * 57000, inactive recombinant precursor enzyme, SDS-PAGE
38000
-
x * 38000, active recombinant enzyme, SDS-PAGE, x * 19000, recombinant isolated propeptide, SDS-PAGE, x * 57000, inactive recombinant precursor enzyme, SDS-PAGE
40000
Bacillus novosp.
-
gel filtration
57000
-
x * 38000, active recombinant enzyme, SDS-PAGE, x * 19000, recombinant isolated propeptide, SDS-PAGE, x * 57000, inactive recombinant precursor enzyme, SDS-PAGE
41000
Bacillus novosp.
-
1 * 41000, SDS-PAGE
41000
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
41000
Bacillus novosp.
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
43000
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
43000
Bacillus novosp.
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
45000
-
1 * 45000
45000
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
45000
Bacillus novosp.
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
64000
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
64000
Bacillus novosp.
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
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?
-
x * 38000, active recombinant enzyme, SDS-PAGE, x * 19000, recombinant isolated propeptide, SDS-PAGE, x * 57000, inactive recombinant precursor enzyme, SDS-PAGE
?
-
x * 38000, active recombinant enzyme, SDS-PAGE, x * 19000, recombinant isolated propeptide, SDS-PAGE, x * 57000, inactive recombinant precursor enzyme, SDS-PAGE
-
?
Bacillus novosp.
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
?
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
-
?
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
?
-
x * 41000, native wild-type enzyme, x * 43000, active recombinant wild-type enzyme, SDS-PAGE, x * 45000, inactive, processed recombinant wild-type enzyme, SDS-PAGE, x * 64000, inactive recombinant precursor enzyme, SDS-PAGE
-
monomer
-
1 * 45000
monomer
Bacillus novosp.
-
1 * 41000, SDS-PAGE
monomer
-
1 * 41000, SDS-PAGE
-
additional information
-
the enzyme consists of an N-terminal prepropeptide and the mature enzyme part
additional information
-
the enzyme consists of an N-terminal prepropeptide and the mature enzyme part
-
additional information
Bacillus novosp.
-
the enzyme consists of an N-terminal prepropeptide and the mature enzyme part
additional information
-
the enzyme consists of an N-terminal prepropeptide and the mature enzyme part
-
additional information
-
the enzyme consists of an N-terminal prepropeptide and the mature enzyme part
additional information
-
the enzyme consists of an N-terminal prepropeptide and the mature enzyme part
-
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D164N
-
site-directed mutagenesis, replacement of the catalytic residue of kumamolisin-As with that of subtilisin, disrupted interaction of Ser278 with residue 164, the mutant shows 1.3% of the wild-type turnover
E78A/D164N
-
site-directed mutagenesis, completely inactive mutant which stays unprocessed
E78H
-
0.01% activity compared to wild-type enzyme, the canonical catalytic triad is disrupted
E78H/D164N
-
site-directed mutagenesis, replacement of the catalytic residues of kumamolisin-As with those of subtilisin, disruption of the catalytic triad, the mutant shows 0.0001% of the wild-type turnover
E78Q/D164N
-
site-directed mutagenesis, completely inactive mutant which stays unprocessed
D164N
-
site-directed mutagenesis, replacement of the catalytic residue of kumamolisin-As with that of subtilisin, disrupted interaction of Ser278 with residue 164, the mutant shows 1.3% of the wild-type turnover
-
E78A/D164N
-
site-directed mutagenesis, completely inactive mutant which stays unprocessed
-
E78H/D164N
-
site-directed mutagenesis, replacement of the catalytic residues of kumamolisin-As with those of subtilisin, disruption of the catalytic triad, the mutant shows 0.0001% of the wild-type turnover
-
E78Q/D164N
-
site-directed mutagenesis, completely inactive mutant which stays unprocessed
-
E78H
-
0.01% activity compared to wild-type enzyme, the canonical catalytic triad is disrupted
-
E32A
Bacillus novosp.
-
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 5.7% of the native enzyme
S278A
Bacillus novosp.
-
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitzo)Phe-Arg-Leu is reduced to 0.5% of the native enzyme
W129A
Bacillus novosp.
-
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 3.8% of the native enzyme
E32A
-
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 5.7% of the native enzyme
-
S278A
-
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitzo)Phe-Arg-Leu is reduced to 0.5% of the native enzyme
-
W129A
-
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 3.8% of the native enzyme
-
D164
-
the mutant shows about 1.3% activity compared to the wild type enzyme
D316A
-
site-directed mutagenesis, autoactivation similar to the wild-type enzyme, 3% of wild-type enzyme activity
D82A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
E78A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
S278A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
D164A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
-
D316A
-
site-directed mutagenesis, autoactivation similar to the wild-type enzyme, 3% of wild-type enzyme activity
-
D82A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
-
E78A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
-
S278A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
-
S278A
an inactive pro-kumamolisin mutant, the catalytic domain of the mutant exhibits a virtually identical structure compared to the active enzyme
S278A
-
an inactive pro-kumamolisin mutant, the catalytic domain of the mutant exhibits a virtually identical structure compared to the active enzyme
-
D164A
-
inactive
D164A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
additional information
Bacillus novosp.
-
mutational analysis of the Ser278 residue reveals that the mutant loses both auto-processing activity and proteolytic activity
additional information
-
mutational analysis of the Ser278 residue reveals that the mutant loses both auto-processing activity and proteolytic activity
-
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Wlodawer, A.; Li, M.; Gustchina, A.; Oyama, H.; Dunn, B.M.; Oda, K.
Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases
Acta Biochim. Pol.
50
81-102
2003
Bacillus novosp., Bacillus novosp. MN-32
brenda
Oda, K.; Ogasawara, S.; Oyama, H.; Dunn, B.M.
Subsite preferences of pepstatin-insensitive carboxyl proteinases from prokaryotes: kumamolysin, a thermostable pepstatin-insensitive carboxyl proteinase
J. Biochem.
128
499-507
2000
Bacillus novosp., Bacillus novosp. MN-32
brenda
Oyama, H.; Hamada, T.; Ogasawara, S.; Uchida, K.; Murao, S.; Beyer, B.B.; Dunn, B.M.; Oda, K.
A CLN2-related and thermostable serine-carboxyl proteinase, kumamolysin: cloning, expression, and identification of catalytic serine residue
J. Biochem.
131
757-765
2002
Bacillus novosp., Bacillus novosp. MN-32
brenda
Murao, S.; Ohkuni, K.; Nagao, M.; Hirayama, K.; Fukuhara, K.; Oda, K.; Oyama, H.; Shin, T.
Purification and characterization of kumamolysin, a novel thermostable pepstatin-insensitive carboxyl proteinase from Bacillus novosp. MN-32
J. Biol. Chem.
268
349-355
1993
Bacillus novosp., Bacillus novosp. MN-32
brenda
Wlodawer, A.; Li, M.; Gustchina, A.; Tsuruoka, N.; Ashida, M.; Minakata, H.; Oyama, H.; Oda, K.; Nishino, T.; Nakayama, T.
Crystallographic and biochemical investigations of kumamolisin-AS a serine-carboxyl peptidase with collagenase activity
J. Biol. Chem.
279
21500-21510
2004
Alicyclobacillus sendaiensis, Alicyclobacillus sendaiensis NTAP-2
brenda
Comellas-Bigler, M.; Fuentes-Prior, P.; Maskos, K.; Huber, R.; Oyama, H.; Uchida, K.; Dunn, B.M.; Oda, K.; Bode, W.
The 1.4 A crystal structure of kumamolysin: a thermostable serine-carboxyl-type proteinase
Structure
10
865-876
2002
Bacillus sp. (in: Bacteria) (Q8RR56), Bacillus sp. (in: Bacteria) MN-32 (Q8RR56)
brenda
Comellas-Bigler, M.; Maskos, K.; Huber, R.; Oyama, H.; Oda, K.; Bode, W.
1.2 A Crystal structure of the serine carboxyl proteinase pro-kumamolisin: structure of an intact pro-subtilase
Structure
12
1313-1323
2004
Bacillus novosp., Bacillus novosp. MN-32
brenda
Fujimoto, Y.; Ikeuchi, H.; Tada, T.; Oyama, H.; Oda, K.; Kunugi, S.
Synergetic effects of pressure and chemical denaturant on protein unfolding: stability of a serine-type carboxyl protease, kumamolisin
Biochim. Biophys. Acta
1764
364-371
2006
Bacillus novosp., Bacillus novosp. MN-32
brenda
Okubo, A.; Li, M.; Ashida, M.; Oyama, H.; Gustchina, A.; Oda, K.; Dunn, B.M.; Wlodawer, A.; Nakayama, T.
Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site
FEBS J.
273
2563-2576
2006
Alicyclobacillus sendaiensis, Alicyclobacillus sendaiensis NTAP-1
brenda
Oda, K.
Kumamolisin
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2nd Ed.
1889-1891
2004
Bacillus novosp., Bacillus novosp. MN-32, Weizmannia coagulans, Weizmannia coagulans J-4
-
brenda
Catara, G.; Fiume, I.; Iuliano, F.; Maria, G.; Ruggiero, G.; Palmieri, G.; Capasso, A.; Rossi, M.
A new kumamolisin-like protease from Alicyclobacillus acidocaldarius: an enzyme active under extreme acidic conditions
Biocatal. Biotransform.
24
358-370
2006
Alicyclobacillus acidocaldarius
-
brenda
Xu, Q.; Li, L.; Guo, H.
Understanding the mechanism of deacylation reaction catalyzed by the serine carboxyl peptidase kumamolisin-As: insights from QM/MM free energy simulations
J. Phys. Chem. B
114
10594-10600
2010
Homo sapiens
brenda
Yao, J.; Wlodawer, A.; Guo, H.
Understanding the autocatalytic process of pro-kumamolisin activation from molecular dynamics and quantum mechanical/molecular mechanical (QM/MM) free-energy simulations
Chem. Eur. J.
19
10849-10852
2013
Bacillus sp. (in: Bacteria) (Q8RR56), Bacillus sp. (in: Bacteria) MN-32 (Q8RR56)
brenda