Information on EC 3.4.21.B48 - kumamolysin

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.4.21.B48
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
kumamolysin
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
specifically hydrolyzes Leu15-Tyr16 peptide bond in oxidized insulin B chain, additional cleavage at Phe25-Tyr26 at a considerably lower rate. Good cleavage of the Phe-I-(p-nitrophenylalanine) bond in synthetic peptides. The enzyme preferentially hydrolyzes peptides having an Ala or Pro residue at P2 position and prefers such charged amino acid residues as Glu or Arg at the P2' position. No cleavage: Asp-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Specifically hydrolyzes Leu15-Tyr16 peptide bond in oxidized insulin B-chain, additional cleavage at Phe25-Tyr26 at a considerably lower rate. Good cleavage of the Phe-/-(p-nitrophenylalanine) bond in synthetic peptides. The enzyme preferentially hydrolyzes peptides having an Ala or Pro residue at P2 position and prefers such charged amino acid residues as Glu or Arg at the P2' position. No cleavage: Asp-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
145380-44-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain NTAP-1, gene scpA
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Manually annotated by BRENDA team
strain J-4
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Manually annotated by BRENDA team
strain J-4
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Manually annotated by BRENDA team
Bacillus novosp.
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Ala-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Arg-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Arg-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Azocoll + H2O
?
show the reaction diagram
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-
-
-
?
Benzyloxycarbonyl-L-Ala-L-Ala-L-Leu 4-nitroanilide + H2O
?
show the reaction diagram
bovine alpha-globulin + H2O
?
show the reaction diagram
-
-
-
-
?
Bovine insulin B-chain + H2O
?
show the reaction diagram
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-
-
-
?
casein + H2O
?
show the reaction diagram
Collagen + H2O
?
show the reaction diagram
-
-
-
-
?
haemoglobin + H2O
?
show the reaction diagram
-
-
-
-
?
high-powder azure + H2O
?
show the reaction diagram
-
-
-
-
?
IQF peptide + H2O
?
show the reaction diagram
Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Leu-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Lys-Ala-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Ala-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Arg-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Arg-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Asp-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Leu-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Leu-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Ala-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Arg-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Asp-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ala-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ala + H2O
Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Ala
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg + H2O
Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Arg
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Asp + H2O
Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Asp
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ser + H2O
Lys-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Ser
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ala-Ser-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Arg-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Arg-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Arg-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Asn-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Asp-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Asp-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Gln-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Gln-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Glu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Glu-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu + H2O
Lys-Pro-Ile-Ala-Phe + Nph-Arg-Leu
show the reaction diagram
Lys-Pro-Ile-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Ala-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ile-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Arg-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Asn-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Asp-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Gln-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ala-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Ala-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ala + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Ala
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Arg + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Arg
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Asp + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Asp
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ile + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Ile
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Nle + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Nle
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Phe
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ser + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Ser
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Tyr + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Tyr
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Val + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Arg-Val
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Asn-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Asp-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Glu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Glu-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Ile-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Leu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Leu-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Lys-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Lys-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ser-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Ser-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu + H2O
Lys-Pro-Ile-Glu-Phe + (p-nitrophenylalanine)-Val-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ile-His-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-His-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Ile-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Ile-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Leu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Pro-Ile-Nle-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Nle-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Pro-Phe-(4-nitrophenyl)-Arg-Leu + H2O
Lys-Pro-Ile-Pro-Phe + (4-nitrophenyl)-Arg-Leu
show the reaction diagram
Lys-Pro-Ile-Pro-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Pro-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Ser-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Thr-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ile-Val-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Leu-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Lys-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Lys-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Phe-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ser-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ser-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Ser-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Thr-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Thr-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
-
-
-
?
Lys-Pro-Val-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Pro-Val-Glu-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Lys-Ser-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Lys-Ser-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
Bacillus novosp.
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-
-
?
Oxidized insulin B-chain + H2O
?
show the reaction diagram
Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu + H2O
Ser-Pro-Ala-Lys-Phe + (p-nitrophenylalanine)-Arg-Leu
show the reaction diagram
[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide + H2O
[2-(N-methylamino)benzoyl]-MGPH + FFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide
show the reaction diagram
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ac-Ile-Ala-Phe-CHO
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synthetic aldehyde inhibitor, binds covalently to the active site, mechanism, overview
Ac-Ile-Pro-Phe-CHO
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synthetic aldehyde inhibitor, binds covalently to the active site, mechanism, overview
acetyl-Ile-Pro-Phe-CHO
Bacillus novosp.
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N-acetyl-Ile-Pro-Phe
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N-acetyl-Ile-Pro-Phe-al
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additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0382
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.103
Arg-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0007 - 0.001
IQF peptide
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0.0269
Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0517
Lys-Ala-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0128
Lys-Arg-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0349
Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0428
Lys-Leu-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0636
Lys-Pro-Ala-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0154
Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.133
Lys-Pro-Ala-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0406
Lys-Pro-Ala-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0567
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ala
Bacillus novosp.
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-
0.0122
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg
Bacillus novosp.
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0.0674
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Asp
Bacillus novosp.
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0.056
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0527
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ser
Bacillus novosp.
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-
0.0645
Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.119
Lys-Pro-Arg-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.142
Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0377
Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0654
Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0351
Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0376
Lys-Pro-Gln-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0404
Lys-Pro-Glu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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0.029 - 0.102
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
0.0426
Lys-Pro-Ile-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0767
Lys-Pro-Ile-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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0.073
Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
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-
0.0405
Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0377
Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0371
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ala-Leu
Bacillus novosp.
-
-
0.0995
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ala
Bacillus novosp.
-
-
0.154
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Arg
Bacillus novosp.
-
-
0.109
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Asp
Bacillus novosp.
-
-
0.0697
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ile
Bacillus novosp.
-
-
0.0603
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.119
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Nle
Bacillus novosp.
-
-
0.0634
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe
Bacillus novosp.
-
-
0.129
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ser
Bacillus novosp.
-
-
0.0248
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Tyr
Bacillus novosp.
-
-
0.0589
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Val
Bacillus novosp.
-
-
0.43
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu
Bacillus novosp.
-
-
0.225
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu
Bacillus novosp.
-
-
0.0438
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Glu-Leu
Bacillus novosp.
-
-
0.0285
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu
Bacillus novosp.
-
-
0.109
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Leu-Leu
Bacillus novosp.
-
-
0.128
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Lys-Leu
Bacillus novosp.
-
-
0.0473
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ser-Leu
Bacillus novosp.
-
-
0.0883
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu
Bacillus novosp.
-
-
0.0831
Lys-Pro-Ile-His-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0128
Lys-Pro-Ile-Ile-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.041
Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0671
Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0468
Lys-Pro-Ile-Nle-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.041
Lys-Pro-Ile-Pro-Phe-(4-nitrophenyl)-Arg-Leu
-
pH 3.5, 60C
0.0409
Lys-Pro-Ile-Pro-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0642
Lys-Pro-Ile-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0447
Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0825
Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0575
Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0246
Lys-Pro-Lys-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0389
Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.1
Lys-Pro-Ser-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0577
Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0469
Lys-Pro-Thr-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.105
Lys-Pro-Val-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.162
Lys-Ser-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.09
oxidized insulin B-chain
Bacillus novosp.
-
pH 3.0, 30C
-
0.0211
Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.0007
[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide
-
mutant enzyme E78H
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.54 - 6.08
Ala-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
0.00045 - 395
IQF peptide
-
0.26
Leu-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.5
Lys-Ala-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
6.08
Lys-Arg-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
1.46 - 6.08
Lys-Asp-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
20.3
Lys-Leu-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
98.3
Lys-Pro-Ala-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.98 - 6.08
Lys-Pro-Ala-Arg-Phe-(p-nitrophenylalanine)-Arg-Leu
0.22
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ala
Bacillus novosp.
-
-
1.04 - 6.08
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Arg
0.19
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Asp
Bacillus novosp.
-
-
0.41
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.45
Lys-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Ser
Bacillus novosp.
-
-
3.5 - 19.9
Lys-Pro-Ala-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu
32.3
Lys-Pro-Arg-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
1.13 - 6.08
Lys-Pro-Arg-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
3.3 - 4.62
Lys-Pro-Asn-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
3 - 6
Lys-Pro-Asp-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
0.64 - 6.08
Lys-Pro-Asp-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
16.1
Lys-Pro-Gln-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
13.1
Lys-Pro-Glu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.08 - 6.08
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
48.5
Lys-Pro-Ile-Ala-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.04 - 9.14
Lys-Pro-Ile-Asn-Phe-(p-nitrophenylalanine)-Arg-Leu
28.6 - 81
Lys-Pro-Ile-Asp-Phe-(p-nitrophenylalanine)-Arg-Leu
3.3 - 4.79
Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu
7.7
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ala-Leu
Bacillus novosp.
-
-
21.7
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Asp
Bacillus novosp.
-
-
12.5
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Ile
Bacillus novosp.
-
-
25.4
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
34.2
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Nle
Bacillus novosp.
-
-
3.7 - 31.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Phe
15.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Tyr
Bacillus novosp.
-
-
13.1
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Val
Bacillus novosp.
-
-
3 - 6
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asn-Leu
0.04 - 8.78
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Asp-Leu
22.7
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Glu-Leu
Bacillus novosp.
-
-
1.82 - 2.94
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ile-Leu
23.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Leu-Leu
Bacillus novosp.
-
-
10.9
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Lys-Leu
Bacillus novosp.
-
-
2.6
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Ser-Leu
Bacillus novosp.
-
-
3.3 - 5.04
Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Val-Leu
3
Lys-Pro-Ile-His-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.3
Lys-Pro-Ile-Ile-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.84 - 6.08
Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu
176
Lys-Pro-Ile-Pro-Phe-(4-nitrophenyl)-Arg-Leu
Bacillus coagulans
-
pH 3.5, 60C
176
Lys-Pro-Ile-Pro-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
52.7
Lys-Pro-Ile-Ser-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
3 - 6
Lys-Pro-Ile-Thr-Phe-(p-nitrophenylalanine)-Arg-Leu
0.73 - 6.08
Lys-Pro-Ile-Val-Phe-(p-nitrophenylalanine)-Arg-Leu
3.13 - 4.44
Lys-Pro-Leu-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
3.13 - 3.76
Lys-Pro-Phe-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
0.052 - 2.1
Lys-Pro-Ser-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.59 - 6.08
Lys-Pro-Ser-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
16.7
Lys-Pro-Thr-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
18.6
Lys-Pro-Val-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
2.35
Lys-Ser-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
71
oxidized insulin B-chain
Bacillus novosp.
-
pH 3.0, 30C
-
0.27
Ser-Pro-Ala-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu
Bacillus novosp.
-
-
0.033
[2-(N-methylamino)benzoyl]-MGPHFFP-Nepsilon-(2,4-dinitrophenyl)-L-Lys-D-Arg-D-Arg-amide
Alicyclobacillus sendaiensis
-
mutant enzyme E78H
additional information
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu
Bacillus novosp.
-
pH 3.5, 60C, wild-type enzyme
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0009
Ac-Ile-Ala-Phe-CHO
-
pH 3.5, 22.4C
0.0007
Ac-Ile-Pro-Phe-CHO
-
pH 3.5, 22.4C
additional information
additional information
Bacillus novosp.
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6600
-
purified recombinant wild-type enzyme
additional information
Bacillus novosp.
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 4
Bacillus novosp.
-
pH 2.5: about 60% of maximal activity, pH 4.0: about 50% of maximal activity, hydrolysis of casein
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50 - 80
Bacillus novosp.
-
50C: about 25% of maximal activity, 80C: about 60% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19000
-
x * 38000, active recombinant enzyme, SDS-PAGE, x * 19000, recombinant isolated propeptide, SDS-PAGE, x * 57000, inactive recombinant precursor enzyme, SDS-PAGE
38000
-
x * 38000, active recombinant enzyme, SDS-PAGE, x * 19000, recombinant isolated propeptide, SDS-PAGE, x * 57000, inactive recombinant precursor enzyme, SDS-PAGE
40000
Bacillus novosp.
-
gel filtration
57000
-
x * 38000, active recombinant enzyme, SDS-PAGE, x * 19000, recombinant isolated propeptide, SDS-PAGE, x * 57000, inactive recombinant precursor enzyme, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no glycoprotein
proteolytic modification
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure of the uninhibited enzyme and its complex with the covalently bound inhibitor, N-acetyl-Ile-Pro-phenylalaninal, vapor diffusion method
-
purified recombinant wild-type enzyme and mutants D164N and E78H/D164N, crystallization buffer contains 0.2 M ammonium sulfate and 30% PEG 8000, pH 4.6, X-ray diffraction structure determination and analysis at 2.0-2.3 A resolution
-
enzyme in complex with inhibitors Ac-Ile-Ala-Phe-CHO and Ac-Ile-Pro-Phe-CHO, X-ray diffraction structure analysis at 1.4 A resolution
-
mutant E32A, W129A and S278A pro-kumamolisin are crystallized by sitting-drop vapor-diffusion method, 1.2/1.3 A crystal structure
Bacillus novosp.
-
crystal structure analysis
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
Bacillus novosp.
-
10 min, stable below
79.4
Bacillus novosp.
-
denaturation temperature
90
Bacillus novosp.
-
10 min, complete loss of activity
additional information
Bacillus novosp.
-
thermostability analysis, denaturation curve, the enzyme is stable against thermal perturbation, 8 M urea reduces thermal stability of the enzyme, decreased cotton effect at 55C, overview
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the enzyme is stable against perturbation by pressure and chemical denaturants
Bacillus novosp.
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
Bacillus novosp.
-
recombinant wild-type and mutant enzymes from Escherichia coli by heat treatment and anion exchange chromatography at pH 7.0, to homogeneity, separation of the propeptide from the mature enzyme by hydroxyapatite chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
Bacillus novosp.
-
expression in Escherichia coli strain JM109
Bacillus novosp.
-
expression of wild-type and mutant enzymes in Escherichia coli strain JM109
-
overexpression of wild-type and mutant enzymes as soluble proteins in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D164N
-
site-directed mutagenesis, replacement of the catalytic residue of kumamolisin-As with that of subtilisin, disrupted interaction of Ser278 with residue 164, the mutant shows 1.3% of the wild-type turnover
E78A/D164N
-
site-directed mutagenesis, completely inactive mutant which stays unprocessed
E78H
-
0.01% activity compared to wild-type enzyme, the canonical catalytic triad is disrupted
E78H/D164N
-
site-directed mutagenesis, replacement of the catalytic residues of kumamolisin-As with those of subtilisin, disruption of the catalytic triad, the mutant shows 0.0001% of the wild-type turnover
E78Q/D164N
-
site-directed mutagenesis, completely inactive mutant which stays unprocessed
D164N
-
site-directed mutagenesis, replacement of the catalytic residue of kumamolisin-As with that of subtilisin, disrupted interaction of Ser278 with residue 164, the mutant shows 1.3% of the wild-type turnover
-
E78A/D164N
-
site-directed mutagenesis, completely inactive mutant which stays unprocessed
-
E78H/D164N
-
site-directed mutagenesis, replacement of the catalytic residues of kumamolisin-As with those of subtilisin, disruption of the catalytic triad, the mutant shows 0.0001% of the wild-type turnover
-
E78Q/D164N
-
site-directed mutagenesis, completely inactive mutant which stays unprocessed
-
E78H
-
0.01% activity compared to wild-type enzyme, the canonical catalytic triad is disrupted
-
D164A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
D316A
-
site-directed mutagenesis, autoactivation similar to the wild-type enzyme, 3% of wild-type enzyme activity
D82A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
E78A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
S278A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
D164A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
-
D316A
-
site-directed mutagenesis, autoactivation similar to the wild-type enzyme, 3% of wild-type enzyme activity
-
D82A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
-
E78A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
-
S278A
-
site-directed mutagenesis, no autoactivation of the proform, inactive mutant
-
E32A
Bacillus novosp.
-
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 5.7% of the native enzyme
S278A
Bacillus novosp.
-
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitzo)Phe-Arg-Leu is reduced to 0.5% of the native enzyme
W129A
Bacillus novosp.
-
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 3.8% of the native enzyme
E32A
-
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 5.7% of the native enzyme
-
S278A
-
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitzo)Phe-Arg-Leu is reduced to 0.5% of the native enzyme
-
W129A
-
the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 3.8% of the native enzyme
-
D164
-
the mutant shows about 1.3% activity compared to the wild type enzyme
D164A
-
inactive
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
-
attractive candidate for industrial-scale biopeptide production under thermoacidophilic conditions