Information on EC 3.4.21.B42 - hippostasin

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.21.B42
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
hippostasin
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
proteolytic cleavage of polypeptides, preferential cleavage site: Arg-
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
342900-22-5
-
9001-01-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene KLK11, glandular kallkrein K11 precursor
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-Arg-Arg-4-methyl-coumaryl-7-amide + H2O
acetyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
recombinant enzyme
-
-
?
acetyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
acetyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Arg-4-methyl-coumaryl-7-amide + H2O
Arg + 7-amino-4-methylcoumarin
show the reaction diagram
recombinant enzyme
-
-
?
benzyl-Arg-4-methyl-coumaryl-7-amide + H2O
benzyl-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
recombinant enzyme
-
-
?
benzyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
benzyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
recombinant enzyme
-
-
?
benzyloxycarbonyl-Gln-Ala-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
recombinant enzyme
-
-
?
benzyloxycarbonyl-Phe-Ser-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
recombinant enzyme
-
-
?
benzyloxycarbonyl-Val-Leu-Lys-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
recombinant enzyme
-
-
?
benzyloxycarbonyl-Val-Pro-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
recombinant enzyme
-
-
?
Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
insulin-like growth factor-binding protein 3 + H2O
?
show the reaction diagram
-
insulin-like growth factor-binding protein 3 is cleaved into 3 fragments of 25000, 20000 and 17000 Da length, respectively
-
-
?
Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
Phe-Ser-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
polypeptide + H2O
peptides
show the reaction diagram
-
-
?
Pro-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
Pro-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
recombinant enzyme
-
-
?
Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
Pro-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Pyr-Gly-Arg-4-methyl-coumaryl-7-amide + H2O
Pyr-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
recombinant enzyme
-
-
?
Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
Val-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
additional information
?
-
-
kallikrein 11 is able to degrade itself
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
polypeptide + H2O
peptides
show the reaction diagram
Q9UBX7
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
amidinophenylmethansulfonylfluorid
-
complete inhibition at 2.5 microM
Aprotinin
-
30% inhibition at 2.5 mM
benzamidine
-
40% inhibition at 2.5 mM
Phenylmethylsulfonylfluoride
-
20 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.27
Gln-Ala-Arg-7-amido-4-methylcoumarin
-
20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C
1.18
Gly-Pro-Arg-7-amido-4-methylcoumarin
-
20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C
0.44
Phe-Ser-Arg-7-amido-4-methylcoumarin
-
20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C
0.53
Pro-Phe-Arg-7-amido-4-methylcoumarin
-
20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C
0.28
Val-Pro-Arg-7-amido-4-methylcoumarin
-
20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.23
Gln-Ala-Arg-7-amido-4-methylcoumarin
Homo sapiens
-
20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C
0.21
Gly-Pro-Arg-7-amido-4-methylcoumarin
Homo sapiens
-
20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C
0.39
Phe-Ser-Arg-7-amido-4-methylcoumarin
Homo sapiens
-
20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C
1.86
Pro-Phe-Arg-7-amido-4-methylcoumarin
Homo sapiens
-
20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C
0.45
Val-Pro-Arg-7-amido-4-methylcoumarin
Homo sapiens
-
20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
KLK11 is upregulated by estrogens, dexamethasone, androgens and progestins in breast cancer cell lines
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
decreased mRNA level compared to nonmalignant lung tissue
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
KLK11 is upregulated by estrogens in cervical cancer cell lines but downregulated by dexamethasone
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
brain-type isozyme
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
decreased mRNA level compared to nonmalignant lung tissue
Manually annotated by BRENDA team
-
gene expression, no significant difference to non-cancerous tissue
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
patients with a high enzyme activity have a longer longer overall survival and progression free survival, expression analysis in 138 cancer samples and 40 healthy controls
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
gene expression
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
especially expressed in secretory epithelial cells; prostate-type isozyme, secreted into the medium
Manually annotated by BRENDA team
-
overexpression of human kallikrein 11 is associated with poor prognosis in patients with low rectal carcinoma
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
-
expression revealed by immunohistochemistry
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
secretion into the medium
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
x * 44000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 40000, SDS-PAGE; x * 44000, SDS-PAGE
monomer
-
SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
4 possible glycosylation sites
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity by immunoaffinity chromatography and high-performance liquid chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Neuro2a cells
-
gene KLK11, identical to gene TLSP or PRSS20, splicing variant 1, prostate-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection; gene KLK11, splicing variant 2, brain-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
both isoforms of KLK11 (prostate-type and brain-type) are significantly higher in cancer tissues compared with their normal counterparts
-
kallikrein 11 expression is significantly decreased in gastric cancer compared with that in normal gastric mucosa
-
kallikrein-related peptidase 11 expression is decreased in prostate cancer cells compared to normal/benign prostate cells
-
regarding prostate-type KLK11, there is a significant association between lower expression and higher tumor stage
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
-
the measurement of kallikrein-related peptidase 11, KLK11, might be a useful diagnostic and prognostic test for non-small cell lung cancer patients. Patients with a high enzyme activity have a longer longer overall survival and progression free survival
medicine