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Information on EC 3.4.21.B41 - kallikrein 10 and Organism(s) Homo sapiens and UniProt Accession O43240
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3.4.21.B41 kallikrein 10Specify your search results
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- 3.4.21.B41
-
kallikreins
- ovarian
- breast
-
prognostic
- carcinoma
- diagnostics
-
methylation-specific
- medicine
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
klk10, human kallikrein 10, kallikrein 10, kallikrein-related peptidase 10, tissue kallikrein-related peptidase 10, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
kallikrein-like protein 1
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-
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KLK-L1
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-
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KLK10
prostase
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-
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S01.246
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY
334993-12-3
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9001-01-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
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kallikrein 10 from asites fluid does not cleave L-Val-Pro-Arg-7-amido-4-methylcoumarin, L-Phe-Ser-Arg-7-amido-4-methylcoumarin, L-Pro-Phe-Arg-7-amido-4-methylcoumarin, L-Val-Leu-Lys-7-amido-4-methylcoumarin, L-Glu-Lys-Lys-7-amido-4-methylcoumarin, and L-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
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-
?
polypeptide + H2O
peptides
activity is down-regulated during oncogenic transformation
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
polypeptide + H2O
peptides
activity is down-regulated during oncogenic transformation
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?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
9-cis-retinoic acid
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treatment of cells with 9-cis-retinoic acid increases the levels of hK10 promoter activity and the levels of hK10 mRNA and protein
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
all K10 present in ovarian cancer ascites fluids are in the uncomplexed, zymogen form and have no detectable enzymatic activity
KLK10 is not methylated in normal breast tissues and fibroadenomas while it is in 5 of 10 breast tumors and in 1 of 10 matching normal tissues
lower KLK10 levels in pleomorphic adenoma suggests aberrant expression in a tumour that develops primarily from myoepithelial cells
normal strain 76N and oncogenically transforming strain 76R-30
kallikreins 10 is abundantly expressed in human oral squamous cell carcinoma and may be implicated in malignant progression
coexpression of KLK10 and KLK6 plays an unfavourable role in pancreatic ductal adenocarcinoma
immunohistochemic enzyme expression analysis in various surgically removed corticotroph adenoma subtypes: lowest enzyme expression in the silent corticotroph subtypes 1 and 2, compared with nontumorous human adenohypophysial corticotrophs, high enzyme expression in endocrinologically active subtypes, ACTH-secreting adenomas, corticotroph carcinomas, Crooke cell adenomas, Crooke cell carcinomas
endocrinologically active adrenocorticotropic hormone (ACTH)-producing pituitary tumors and the silent subtypes are immunopositve for kallikrein 10. Immunoexpression of kallikrein 10 in the ACTH-secreting tumors as well as in the Crooke cell tumors is significantly increased when compared with the nonfunctioning tumors and in the corticotrophs of nontumorous pituitaries
from malignant tumours, adenoid cystic carcinoma and mucoepidermoid carcinoma
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hypermethylated KLK10 is frequent in hepatocellular carcinoma
KLK10 is significantly downregulated as compared to non-cancer samples. CpG island hypermethylation of KLK10 is detected in 46.2% of non-small cell lung cancer tissues
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increased mRNA expression compared to normal cells
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increased mRNA expression compared to noncancer cells
additional information
expression in normal cells, but no expression in breast cancer cells
KLK10 is not methylated in normal breast tissues and fibroadenomas while it is in 5 of 10 breast tumors and in 1 of 10 matching normal tissues
additional information
gene expression is almost lost in breast cancer cell lines
additional information
no expression in fibroblasts, peripheral blood leukocytes, brain, placenta, lung, liver, skeletal muscle, and kidney
additional information
significantly increased enzyme expression in dysplastic lesions compared with metaplasia but not between dysplastic lesions and invasive cancers, immunohistochemic analysis, overview
additional information
-
significantly increased enzyme expression in dysplastic lesions compared with metaplasia but not between dysplastic lesions and invasive cancers, immunohistochemic analysis, overview
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
the enzyme is a member of the human kallikrein gene family of secreted serine proteases
metabolism
malfunction
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KLK10 hypermethylation is significantly associated with cirrhosis and HCV infection as well as inversely associated with HBV infection. Restoration of KLK10 expression, by 5-aza-cytidine treatment, reduces the ability of anchorage-independent growth, and sensitizes hepatocellular carcinoma cells to doxorubicin- or 5-fluorouracil-induced cytotoxicity
additional information
metabolism
multiple mechanisms account for enzyme KLK10 dysregulation in cancer including hypermethylation, and the frequent inactivation and loss of KLK10 expression is a critical step towards carcinogenesis
metabolism
the enzyme is mplicated in the development of several types of cancer. A kallikrein cascade may play a role in the development and/or outcome of some salivary gland tumours, overview
additional information
significant correlation between KLK6 and KLK10 expression both at the invasive front and within the main tumor, indicating a collaborative effect
additional information
-
significant correlation between KLK6 and KLK10 expression both at the invasive front and within the main tumor, indicating a collaborative effect
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). KLK10_HUMAN
276
0
30170
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
reverse-phase high-performance liquid chromatography and immunoaffinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene KLK10, determination of frequency and functional significance of KLK10 hypermethylation in non-small cell lung cancer cells, mRNA expression and methylation status of KLK10 in 78 pairs specimens, overview
gene KLK10, located at 19q13.3-q13.4, quantitative real-time PCR expression analysis, determination of DNA hypermethylation status of KLK10 in hepatocellular carcinoma cell lines and clinical samples, DNA methylation profiles, overview. Ectopic expression of KLK10 enhances drug sensitivity of HuH7 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is up-regulated in colorectal cancer, stepwise expression increase from metaplasia to dysplasia and invasive tumors, significantly increased enzyme expression in early invasive cancer, overview
frequent transcriptional inactivation of kallikrein 10 gene by CpG island hypermethylation in non-small cell lung cancer, overview. Restoration of KLK10 expression by 5-Aza-dC treatment
KLK10 expression is significantly higher in cancerous than in normal colorectal tissues
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
diagnostics
diagnostics
medicine
diagnostics
enzyme KLK10 is significantly associated with other prognostic markers in gastroesophageal junction adenocarcinoma and Barrett esophagus, including depth of invasion, indicating its potential as independent biomarker
diagnostics
KLK10 overexpression correlates with poor prognosis in colorectal cancer, being associated with liver metastases, decreased disease-free, and overall survival. Negative correlation between KLK10 expression and tumor grade, indicating that these two parameters. KLK10 expression, assessed by immunohistochemistry, is an independent indicator of poor prognosis. Assessment of KLK10 can be incorporatedto a multi-molecular prognostic model to achieve better and more accurate assessment of disease prognosis are affected by independent mechanisms
diagnostics
elevated tumor tissue protein expression levels of kallikrein-related peptidases KLK10 and KLK11 are associated with a favorable prognosis in advanced high-grade serous ovarian cancer patients
diagnostics
urinary KLK10 is a promising non-invasive biomarker for the inoperability and incurability of gastric cancer
diagnostics
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hypermethylated KLK10 is frequent in hepatocellular carcinoma and may be useful as marker for clinical application
diagnostics
KLK10 methylation can be used as a biomarker for non-small cell lung cancer
medicine
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kallikrein 10 may serve as a biomarker for uterine serous papillary carcinoma
medicine
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high status KLK10 expression is a significant factor for disease-free survival and overall survival of colorectal cancer patients. KLK10 gene expression may be used as a marker of unfavorable prognosis for colorectal cancer
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Homo sapiens (O43240)
Liu, X.L.; Wazer, D.E.; Watanabe, K.; Band, V.
Identification of a novel serine protease-like gene, the expression of which is down-regulated during breast cancer progression
Cancer Res.
56
3371-3379
1996
Homo sapiens (O43240)
Santin, A.D.; Diamandis, E.P.; Bellone, S.; Marizzoni, M.; Bandiera, E.; Palmieri, M.; Papasakelariou, C.; Katsaros, D.; Burnett, A.; Pecorelli, S.
Overexpression of kallikrein 10 (hK10) in uterine serous papillary carcinomas
Am. J. Obstet. Gynecol.
194
1296-1302
2006
Homo sapiens
Zhang, Y.; Bhat, I.; Zeng, M.; Jayal, G.; Wazer, D.E.; Band, H.; Band, V.
Human kallikrein 10, a predictive marker for breast cancer
Biol. Chem.
387
715-721
2006
Homo sapiens
Planque, C.; Ainciburu, M.; Heuze-Vourch, N.; Regina, S.; de Monte, M.; Courty, Y.
Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues
Biol. Chem.
387
783-788
2006
Homo sapiens
Yousef, G.M.; White, N.M.; Michael, I.P.; Cho, J.C.; Robb, J.D.; Kurlender, L.; Khan, S.; Diamandis, E.P.
Identification of new splice variants and differential expression of the human kallikrein 10 gene, a candidate cancer biomarker
Tumour Biol.
26
227-235
2005
Homo sapiens (O43240), Homo sapiens
Sidiropoulos, M.; Pampalakis, G.; Sotiropoulou, G.; Katsaros, D.; Diamandis, E.P.
Downregulation of human kallikrein 10 (KLK10/NES1) by CpG island hypermethylation in breast, ovarian and prostate cancers
Tumour Biol.
26
324-336
2005
Homo sapiens
Petraki, C.D.; Gregorakis, A.K.; Vaslamatzis, M.M.; Papanastasiou, P.A.; Yousef, G.M.; Levesque, M.A.; Diamandis, E.P.
Prognostic Implications of the Immunohistochemical Expression of Human Kallikreins 5, 6, 10 and 11 in Renal Cell Carcinoma
Tumour Biol.
27
1-7
2006
Homo sapiens
Zeng, M.; Zhang, Y.; Bhat, I.; Wazer, D.E.; Band, H.; Band, V.
The human kallikrein 10 promoter contains a functional retinoid response element
Biol. Chem.
387
741-747
2006
Homo sapiens
Kulasingam, V.; Diamandis, E.P.
Glucocorticoid receptor-mediated expression of kallikrein 10 in human breast cancer cell lines
Biol. Chem.
388
1113-1119
2007
Homo sapiens
Ewan King, L.; Li, X.; Cheikh Saad Bouh, K.; Pedneault, M.; Chu, C.W.
Human kallikrein 10 ELISA development and validation in breast cancer sera
Clin. Biochem.
40
1057-1062
2007
Homo sapiens
Feng, B.; Xu, W.B.; Zheng, M.H.; Ma, J.J.; Cai, Q.; Zhang, Y.; Ji, J.; Lu, A.G.; Qu, Y.; Li, J.W.; Wang, M.L.; Hu, W.G.; Liu, B.Y.; Zhu, Z.G.
Clinical significance of human kallikrein 10 gene expression in colorectal cancer and gastric cancer
J. Gastroenterol. Hepatol.
21
1596-1603
2006
Homo sapiens
Luo, L.; Soosaipillai, A.; Grass, L.; Diamandis, E.P.
Characterization of human kallikreins 6 and 10 in ascites fluid from ovarian cancer patients
Tumour Biol.
27
227-234
2006
Homo sapiens (O43240)
Kioulafa, M.; Kaklamanis, L.; Stathopoulos, E.; Mavroudis, D.; Georgoulias, V.; Lianidou, E.S.
Kallikrein 10 (KLK10) methylation as a novel prognostic biomarker in early breast cancer
Ann. Oncol.
20
1020-1025
2009
Homo sapiens (O43240), Homo sapiens
Rueckert, F.; Hennig, M.; Petraki, C.D.; Wehrum, D.; Distler, M.; Denz, A.; Schroeder, M.; Dawelbait, G.; Kalthoff, H.; Saeger, H.D.; Diamandis, E.P.; Pilarsky, C.; Gruetzmann, R.
Co-expression of KLK6 and KLK10 as prognostic factors for survival in pancreatic ductal adenocarcinoma
Br. J. Cancer
99
1484-1492
2008
Homo sapiens (O43240)
Pettus, J.R.; Johnson, J.J.; Shi, Z.; Davis, J.W.; Koblinski, J.; Ghosh, S.; Liu, Y.; Ravosa, M.J.; Frazier, S.; Stack, M.S.
Multiple kallikrein (KLK 5, 7, 8, and 10) expression in squamous cell carcinoma of the oral cavity
Histol. Histopathol.
24
197-207
2009
Homo sapiens (O43240)
Zhang, Y.; Song, H.; Miao, Y.; Wang, R.; Chen, L.
Frequent transcriptional inactivation of Kallikrein 10 gene by CpG island hypermethylation in non-small cell lung cancer
Cancer Sci.
101
934-940
2010
Homo sapiens (Q6ICR6), Homo sapiens
Lu, C.Y.; Hsieh, S.Y.; Lu, Y.J.; Wu, C.S.; Chen, L.C.; Lo, S.J.; Wu, C.T.; Chou, M.Y.; Huang, T.H.; Chang, Y.S.
Aberrant DNA methylation profile and frequent methylation of KLK10 and OXGR1 genes in hepatocellular carcinoma
Genes Chromosomes Cancer
48
1057-1068
2009
Homo sapiens
Talieri, M.; Alexopoulou, D.K.; Scorilas, A.; Kypraios, D.; Arnogiannaki, N.; Devetzi, M.; Patsavela, M.; Xynopoulos, D.
Expression analysis and clinical evaluation of kallikrein-related peptidase 10 (KLK10) in colorectal cancer
Tumour Biol.
32
737-744
2011
Homo sapiens
Di Meo, A.; Rotondo, F.; Kovacs, K.; Cusimano, M.D.; Syro, L.V.; Di Ieva, A.; Diamandis, E.P.; Yousef, G.M.
Human kallikrein 10 expression in surgically removed human pituitary corticotroph adenomas: an immunohistochemical study
Appl. Immunohistochem. Mol. Morphol.
23
433-437
2014
Homo sapiens (O43240), Homo sapiens
Grin, A.; Samaan, S.; Tripathi, M.; Rotondo, F.; Kovacs, K.; Bassily, M.N.; Yousef, G.M.
Evaluation of human tissue kallikrein-related peptidases 6 and 10 expression in early gastroesophageal adenocarcinoma
Hum. Pathol.
46
541-548
2015
Homo sapiens (O43240), Homo sapiens
Darling, M.R.; Hashem, N.N.; Zhang, I.; Mohamed, A.B.; Fung, K.; Kwan, K.; Mara, T.W.; Daley, T.D.; Diamandis, E.P.
Kallikrein-related peptidase 10 expression in salivary gland tissues and tumours
Int. J. Biol. Markers
27
e381-e388
2013
Homo sapiens (O43240)
Petraki, C.; Youssef, Y.M.; Dubinski, W.; Lichner, Z.; Scorilas, A.; Pasic, M.D.; Komborozos, V.; Khalil, B.; Streutker, C.; Diamandis, E.P.; Yousef, G.M.
Evaluation and prognostic significance of human tissue kallikrein-related peptidase 10 (KLK10) in colorectal cancer
Tumour Biol.
33
1209-1214
2012
Homo sapiens (O43240), Homo sapiens
Geng, X.; Liu, Y.; Dreyer, T.; Bronger, H.; Drecoll, E.; Magdolen, V.; Dorn, J.
Elevated tumor tissue protein expression levels of kallikrein-related peptidases KLK10 and KLK11 are associated with a better prognosis in advanced high-grade serous ovarian cancer patients
Am. J. Cancer Res.
8
1856-1864
2018
Homo sapiens (O43240), Homo sapiens
Di Meo, A.; Rotondo, F.; Kovacs, K.; Cusimano, M.; Syro, L.; Di Ieva, A.; Diamandis, E.; Yousef, G.
Human kallikrein 10 expression in surgically removed human pituitary corticotroph adenomas an immunohistochemical study
Appl. Immunohistochem. Mol. Morphol.
23
433-437
2015
Homo sapiens (O43240), Homo sapiens
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