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Information on EC 3.4.21.B40 - kallikrein 9 and Organism(s) Homo sapiens and UniProt Accession Q9UKQ9

for references in articles please use BRENDA:EC3.4.21.B40
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B40 kallikrein 9
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This record set is specific for:
Homo sapiens
UNIPROT: Q9UKQ9
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
proteolytic cleavage of polypeptides, preferential cleavage site at paired basic amino acid residues
Synonyms
egf-bp, epidermal growth factor-binding protein, egf-binding protein, hmw-egf, kallikrein 9, epidermal growth factor urogastrone, high molecular weight epidermal growth factor, human tissue kallikrein 9, s3 kallikrein, kallikrein-related peptidase 9, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tissue kallikrein 9
-
human tissue kallikrein 9
-
-
kallikrein 9
-
-
S01.307
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
9001-01-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
AAPF-7-amido-4-methylcoumarin + H2O
AAPF + 7-amino-4-methylcoumarin
show the reaction diagram
15% activity compared to LLVY-7-amido-4-methylcoumarin
-
-
?
LLVY-7-amido-4-methylcoumarin + H2O
LLVY + 7-amino-4-methylcoumarin
show the reaction diagram
preferred substrate
-
-
?
midkine + H2O
?
show the reaction diagram
-
-
-
?
polypeptide + H2O
peptides
show the reaction diagram
-
-
?
tissue kallikrein 10 + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
polypeptide + H2O
peptides
show the reaction diagram
-
-
?
additional information
?
-
enzyme is regulated by steroid hormones in breast cancer cell line BT-474
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
about 180% activity at 25 mM
Mg2+
about 220% activity at 12.5 mM
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Aprotinin
16% inhibition at 0.001 mM
leupeptin
38% inhibition at 0.001 mM
phenylmethylsulfonyl fluoride
almost complete inhibition at 10 mM
serpin A3
most potent serpin inhibitor, complete inhibition at 0.001 mM
-
serpin A5
about 40% inhibition at 0.001 mM
-
serpin C1
about 60% inhibition at 0.001 mM
-
serpin F2
about 35% inhibition at 0.001 mM
-
Zn2+
reversible inhibition. 5 mM ZnCl2 almost completely abolishes the enzyme activity. EDTA (final concentration of 50 mM) completely reverses the Zn2+ inhibition
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.07
LLVY-7-amido-4-methylcoumarin
at pH 8.0 and 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.34
LLVY-7-amido-4-methylcoumarin
at pH 8.0 and 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.298
with LLVY-7-amido-4-methylcoumarin as substrate, at pH 8.0 and 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
more than 40% activity between pH 7.0 and 10.0
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low content
Manually annotated by BRENDA team
high content in adult, low content in fetal brain
Manually annotated by BRENDA team
low content
Manually annotated by BRENDA team
low content
Manually annotated by BRENDA team
low content
Manually annotated by BRENDA team
low content
Manually annotated by BRENDA team
additional information
no enzyme expression in uterus, heart, adrenal gland, colon, spleen, skeletal muscle, pancreas, kidney, and fetal liver
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KLK9_HUMAN
250
0
27513
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
-
SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
at 37°C, the enzyme retains about 75% and 50% activity after 6-12 h and 48 h, respectively
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Mono Q column chromatography and Viva C4 column chromatography
Ni-affinity chromatography to homogeneity
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, gene is part of the human kallikrein multigene family, that mapps on chromosome 19q13.3-q13.4
expressed in Expi293 cells
His-tag fuision protein, expression in Escherichia coli BL21(DE3)
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
the ELISAs for free and bound forms of KLK9 may be highly useful for the detection of KLK9 in a broad range of biological samples, thus enabling the clarification of KLK9 function and use as a potential disease biomarker
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Homo sapiens (Q9UKQ9), Mus musculus (P15949), Rattus norvegicus (P07647)
Manually annotated by BRENDA team
Yousef, G.M.; Diamandis, E.P.
The expanded human kallikrein gene family: locus characterization and molecular cloning of a new member, KLK-L3 (KLK9)
Genomics
65
184-194
2000
Homo sapiens (Q9UKQ9)
Manually annotated by BRENDA team
Tan, O.L.; Whitbread, A.K.; Clements, J.A.; Dong, Y.
Kallikrein-related peptidase (KLK) family mRNA variants and protein isoforms in hormone-related cancers: do they have a function?
Biol. Chem.
387
697-705
2006
Homo sapiens
Manually annotated by BRENDA team
Memari, N.; Grass, L.; Nakamura, T.; Karakucuk, I.; Diamandis, E.P.
Human tissue kallikrein 9: production of recombinant proteins and specific antibodies
Biol. Chem.
387
733-740
2006
Homo sapiens
Manually annotated by BRENDA team
Filippou, P.S.; Farkona, S.; Brinc, D.; Yu, Y.; Prassas, I.; Diamandis, E.P.
Biochemical and functional characterization of the human tissue kallikrein 9
Biochem. J.
474
2417-2433
2017
Homo sapiens (Q9UKQ9), Homo sapiens
Manually annotated by BRENDA team
Filippou, P.; Korbakis, D.; Farkona, S.; Soosaipillai, A.; Karakosta, T.; Diamandis, E.P.
A new enzyme-linked immunosorbent assay (ELISA) for human free and bound kallikrein 9
Clin. Proteomics
14
4
2017
Homo sapiens (Q9UKQ9), Homo sapiens
Manually annotated by BRENDA team