Information on EC 3.4.21.B40 - kallikrein 9

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.21.B40
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
kallikrein 9
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
proteolytic cleavage of polypeptides, preferential cleavage site at paired basic amino acid residues
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
9001-01-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
three isozymes, encoded by (CANFA)KLK9 genes
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzyloxycarbonyl-Arg-Val-Arg-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low activity
-
-
?
benzyloxycarbonyl-Gln-Arg-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Glu-Lys-Lys-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
low activity
-
-
?
benzyloxycarbonyl-Leu-Lys-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
D-Val-Leu-Arg-NHPhNO2
?
show the reaction diagram
-
-
-
-
-
gurmarin + H2O
?
show the reaction diagram
-
-
-
-
?
L-kininogen + H2O
kinin + ?
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester
?
show the reaction diagram
-
-
-
-
-
Nalpha-p-Tosyl-L-arginine methyl ester
?
show the reaction diagram
-
-
-
-
-
o-aminobenzoyl-SVIRRVQ-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-TSVIRRPQ-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
p-toluenesulfonyl-L-arginine methyl ester + H2O
p-toluenesulfonyl-L-arginine + methyl alcohol
show the reaction diagram
-
-
-
-
?
polypeptide + H2O
peptides
show the reaction diagram
pre-[epidermal growth factor] + H2O
epidermal growth factor + ?
show the reaction diagram
-
-
-
-
?
prorenin 2 + H2O
renin 2 + ?
show the reaction diagram
Pyr-Arg-Tyr-Lys-Arg-4-methyl-coumaryl-7-amide + H2O
Pyr-Arg-Tyr-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
best substrate
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
polypeptide + H2O
peptides
show the reaction diagram
pre-[epidermal growth factor] + H2O
epidermal growth factor + ?
show the reaction diagram
-
-
-
-
?
additional information
?
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Q9UKQ9
enzyme is regulated by steroid hormones in breast cancer cell line BT-474
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-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0016
o-aminobenzoyl-TSVIRRVQ-ethylenediamine
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0005
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purified enzyme, substrate Pyr-Arg-Tyr-Lys-Arg-4-methyl-coumaryl-7-amide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low content
Manually annotated by BRENDA team
high content in adult, low content in fetal brain
Manually annotated by BRENDA team
low content
Manually annotated by BRENDA team
low content
Manually annotated by BRENDA team
low content
Manually annotated by BRENDA team
low content
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7000
-
1 * 7000 + 1 * 10000, reducing SDS-PAGE
10000
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1 * 7000 + 1 * 10000, reducing SDS-PAGE
17000
-
SDS-PAGE
45000
-
SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
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1 * 7000 + 1 * 10000, reducing SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE column chromatography
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Ni-affinity chromatography to homogeneity
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, gene is part of the human kallikrein multigene family, that mapps on chromosome 19q13.3-q13.4
gene (CANFA)KLK9, DNA and amino acid sequence determination and analysis, genetic structure and splicing events, overview. Sequence comparison with the human gene, detailed overview
His-tag fuision protein, expression in Escherichia coli BL21(DE3)
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