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Information on EC 3.4.21.B4 - granzyme K and Organism(s) Mus musculus and UniProt Accession O35205

for references in articles please use BRENDA:EC3.4.21.B4
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B4 granzyme K
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This record set is specific for:
Mus musculus
UNIPROT: O35205
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Word Map
  • 3.4.21.B4
  • perforin
  • spastic
  • paraplegia
  • oxysterols
  • neurosteroids
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
endopeptidase activity. Cleaves after Lys and Arg
Synonyms
granzyme k, fragmentin, granzyme 3, rnk-tryp-2, gzm k, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
granzyme K
-
-
granzyme tryptase K
-
-
GzmK
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
cleavage of C-N-linkage
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
196622-94-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Nalpha-benzyloxycarbonyl-L-Arg-thiobenzyl ester
?
show the reaction diagram
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Lys-thiobenzyl ester
?
show the reaction diagram
-
-
-
?
Protein + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Protein + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
granzyme K expressing cytotoxic T lymphocytes protects against influenza virus in granzyme AB-/- mice. GrzK plays an important role in CD8+ T-cell cytotoxicity both in the presence and absence of grzA and B
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-aminobenzamidine
-
57% inactivation at 5 mM
Aprotinin
-
89% inactivation at 0.005 mM
benzamidine
-
57% inactivation at 27 mM
bikunin
-
light chain of inter-alpha-trypsin inhibitor
-
Glu-Gly-Arg-chloromethyl ketone
-
52% inactivation at 0.1 mM
leupeptin
-
22% inactivation at 0.2 mM
PefablocSC
-
94% inactivation at 1 mM
Phe-Pro-Arg-chloromethyl ketone
-
complete inactivation at 0.1 mM
second carboxy-terminal Kunitz-type domain of bikunin
-
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000033
bikunin
-
pH 7.6
-
0.000022
second carboxy-terminal Kunitz-type domain of bikunin
-
pH 7.6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
GzmK influences wound healing by augmenting inflammation and impeding epithelialization. GzmK-/- mice exhibit improved wound closure, matrix organization, and tensile strength compared with wild-type mice. Reduced proinflammatory IL-6, ICAM-1, VCAM-1, and MCP-1 expressions are observed at 3 days after injury. GzmK induces IL-6 expression in keratinocytes and skin fibroblasts that is dependent on PAR-1 activation. Keratinocytes, but not skin fibroblasts, exposed to GzmK show impaired wound healing in vitro
physiological function
-
the enzyme has a role in cytotoxic and inflammatory processes
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GRAK_MOUSE
263
0
29251
Swiss-Prot
other Location (Reliability: 2)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wilharm, E.; Tschopp, J.; Jenne, D.E.
Biological activities of granzyme K are conserved in the mouse and account for residual Z-Lys-SBzl activity in granzyme A-deficient mice
FEBS Lett.
459
139-142
1999
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Babe, L.M.; Schmidt, B.F.
Granzyme K
Handbook of Proteolytic Enzymes (Barrett,A. J. ;Rawlings,N. D. ,Woessner)
2
1552-1554
2004
Mus musculus
-
Manually annotated by BRENDA team
Jenkins, M.R.; Trapani, J.A.; Doherty, P.C.; Turner, S.J.
Granzyme K expressing cytotoxic T lymphocytes protects against influenza virus in granzyme AB-/- mice
Viral Immunol.
21
341-346
2008
Mus musculus (O35205)
Manually annotated by BRENDA team
Plasman, K.; Demol, H.; Bird, P.I.; Gevaert, K.; Van Damme, P.
Substrate specificities of the granzyme tryptases A and K
J. Proteome Res.
13
6067-6077
2014
Homo sapiens (P49863), Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Turner, C.T.; Zeglinski, M.R.; Richardson, K.C.; Zhao, H.; Shen, Y.; Papp, A.; Bird, P.I.; Granville, D.J.
Granzyme K expressed by classically activated macrophages contributes to inflammation and impaired remodeling
J. Invest. Dermatol.
139
930-939
2019
Homo sapiens (P49863), Homo sapiens, Mus musculus (O35205)
Manually annotated by BRENDA team