Information on EC 3.4.21.B39 - stratum corneum tryptic enzyme

Word Map on EC 3.4.21.B39
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.21.B39
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
stratum corneum tryptic enzyme
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
proteolytic cleavage of polypeptides
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
272788-46-2
-
9001-01-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
KLK5 is part of an original biological cascade in which unregulated KLK5 directly activates proteinase-activated receptor 2 and induces nuclear factor kappaB-mediated overexpression of TSLP, intercellular adhesion molecule 1, tumor necrosis factor alpha, and IL8. This proinflammatory and proallergic pathway is independent of the primary epithelial failure and is activated under basal conditions in NS keratinocytes
physiological function
additional information
-
potential role of endogenous KLK5 expression in supporting CaP cells to cope with the cytostatic phenomena induced by chemotherapeutic agents practiced in standard first-line CaP chemotherapy
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Abz-APGRVVGGA-Q-N-[2,4-dinitrophenyl]-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLRSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Ala-Thr-Pro-Lys-Ile-Phe-Asn + H2O
Ala-Thr-Pro-Lys + Ile-Phe-Asn
show the reaction diagram
-
moderate cleavage efficiency
-
-
?
Asp-Gly-Asp-Lys-Ile-Ile-Glu + H2O
Asp-Gly-Asp-Lys + Ile-Ile-Glu
show the reaction diagram
-
low cleavage efficiency
-
-
?
Asp-Thr-Arg-Ala-Ile-Gly + H2O
Asp-Thr-Arg + Ala-Ile-Gly
show the reaction diagram
-
moderate cleavage efficiency
-
-
?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Boc-Gln-Ala-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Gln-Ala-Arg + 7-amino-4-methyl-coumarin
show the reaction diagram
-
-
-
-
?
Boc-Gln-Arg-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Gln-Arg-Arg + 7-amino-4-methyl-coumarin
show the reaction diagram
-
-
-
-
?
Boc-Gln-Gly-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Gln-Gly-Arg + 7-amino-4-methyl-coumarin
show the reaction diagram
-
-
-
-
?
Boc-Leu-Arg-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Leu-Arg-Arg + 7-amino-4-methyl-coumarin
show the reaction diagram
-
-
-
-
?
Boc-Leu-GLy-Arg-7-amido-4-methylcoumarin + H2O
Boc-Leu-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Boc-Leu-Lys-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Leu-Lys-Arg + 7-amino-4-methyl-coumarin
show the reaction diagram
-
-
-
-
?
Boc-Phe-Ser-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Phe-Ser-Arg + 7-amino-4-methyl-coumarin
show the reaction diagram
-
-
-
-
?
Boc-Val-Leu-Lys-7-amido-4-methyl-coumarin + H2O
Boc-Val-Leu-Lys + 7-amino-4-methyl-coumarin
show the reaction diagram
-
-
-
-
?
Boc-Val-Pro-Arg-7-amido-4-methyl-coumarin + H2O
Boc-Val-Pro-Arg + 7-amino-4-methyl-coumarin
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
Cbz-L-Pro-Phe-Arg-p-nitroanilide + H2O
Cbz-L-Pro-Phe-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
collagen type IV + H2O
?
show the reaction diagram
-
-
-
-
?
D-Ile-Pro-Arg-p-nitroanilide + H2O
D-Ile-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
defensin-1alpha + H2O
?
show the reaction diagram
-
KLK5 is capable of processing defensin-1alpha and may be important in vaginal host defense
-
-
?
desmocollin-2 + H2O
?
show the reaction diagram
-
-
-
-
?
desmocollin-3 + H2O
?
show the reaction diagram
-
-
-
-
?
desmoglein 1 + H2O
?
show the reaction diagram
-
-
-
-
?
desmoglein protein 1 + H2O
?
show the reaction diagram
-
-
-
-
?
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin + H2O
diglutamyl-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
diglutamyl-Pro-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Fibrinogen + H2O
?
show the reaction diagram
-
-
-
-
?
fibrinogen Aalpha chain + H2O
?
show the reaction diagram
-
cleavage after Arg348 and Arg407
-
-
?
fibrinogen Bbeta chain + H2O
?
show the reaction diagram
-
cleavage after Lys53 and Arg14
-
-
?
Fibronectin + H2O
?
show the reaction diagram
-
-
-
-
?
FSR-amido-4-methylcoumarin + H2O
FSR + 7-amino-4-methylcoumarin
show the reaction diagram
fluorogenic trypsin-like peptide substrate
-
-
?
Gelatin + H2O
?
show the reaction diagram
Gln-Glu-Asp-Lys-Val-Leu-Gly + H2O
Gln-Glu-Asp-Lys + Val-Leu-Gly
show the reaction diagram
-
low cleavage efficiency
-
-
?
Gln-Gly-Asp-Lys-Ile-Ile-Asp + H2O
Gln-Gly-Asp-Lys + Ile-Ile-Asp
show the reaction diagram
-
low cleavage efficiency
-
-
?
Glu-Thr-Arg-Ile-Ile-Lys + H2O
Glu-Thr-Arg + Ile-Ile-Lys
show the reaction diagram
-
moderate cleavage efficiency
-
-
?
high-molecular-weight kininogen + H2O
?
show the reaction diagram
-
-
-
-
?
hK3 + H2O
?
show the reaction diagram
-
hK5 can inactivate hK3 by subsequent cleavage after activation
-
-
-
human plasminogen + H2O
human plasmin + ?
show the reaction diagram
-
generation of a 29 kDa fragment
-
-
?
Ile-Gln-Ser-Arg-Ile-Val-Gly + H2O
Ile-Gln-Ser-Arg + Ile-Val-Gly
show the reaction diagram
-
high cleavage efficiency
-
-
?
Ile-Leu-Ser-Arg-Ile-Val
Ile-Leu-Ser-Arg + Ile-Val
show the reaction diagram
-
optimal substrate sequences for KLK5
-
-
-
Ile-Leu-Ser-Arg-Ile-Val-Gly + H2O
Ile-Leu-Ser-Arg + Ile-Val-Gly
show the reaction diagram
-
high cleavage efficiency
-
-
?
insulin-like growth factor-binding protein 1 + H2O
insulin-like growth factor-binding protein 1a + insulin-like growth factor-binding protein 1b
show the reaction diagram
-
cleavage after Lys137
-
-
?
insulin-like growth factor-binding protein 1 + H2O
insulin-like growth factor-binding protein 4a + insulin-like growth factor-binding protein 4b
show the reaction diagram
-
cleavage after Arg170
-
-
?
insulin-like growth factor-binding protein 2 + H2O
insulin-like growth factor-binding protein 2a + insulin-like growth factor-binding protein 2b
show the reaction diagram
-
cleavage after Arg164
-
-
?
insulin-like growth factor-binding protein 3 + H2O
insulin-like growth factor-binding protein 3a + insulin-like growth factor-binding protein 3b + insulin-like growth factor-binding protein 3c
show the reaction diagram
-
cleavage after Arg158 and Arg206
-
-
?
insulin-like growth factor-binding protein 5 + H2O
insulin-like growth factor-binding protein 5a + insulin-like growth factor-binding protein 5b + insulin-like growth factor-binding protein 5c
show the reaction diagram
-
cleavage after Arg123 and Arg212
-
-
?
insulin-like growth factor-binding protein-3 + H2O
?
show the reaction diagram
-
-
-
-
?
kallikrein 7 + H2O
?
show the reaction diagram
-
Gln-Gly-Asp-Lys-/-Ile-Ile. KLK7 is activated at a low rate
-
-
?
kininogen + H2O
?
show the reaction diagram
-
cleavage after Arg222, Arg252 and Arg374
-
-
?
L-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
L-Pro-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
mucin 4 + H2O
?
show the reaction diagram
-
-
-
-
?
mucin 5B + H2O
?
show the reaction diagram
-
-
-
-
?
N(p-tosyl)-Arg-Gly-Val 4-nitroanilide
?
show the reaction diagram
-
-
-
-
?
plasminogen + H2O
?
show the reaction diagram
-
cleavage after Lys77 andA rg529
-
-
?
polypeptide + H2O
peptides
show the reaction diagram
pro-hK2 + H2O
hK2 + ?
show the reaction diagram
-
hK5 can activate hK2 through cleavage after Lys137
-
-
?
pro-hK3 + H2O
hK3 + ?
show the reaction diagram
-
hK5 can activate hK3 through cleavage after Arg85 and Lys182
-
-
?
Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin + H2O
Pro-Phe-Ser-Arg + 7-amino-4-methyl-coumarin
show the reaction diagram
-
-
-
-
?
pro-[stratum corneum chymotryptic enzyme] + H2O
stratum corneum chymotryptic enzyme + ?
show the reaction diagram
-
stratum corneum tryptic enzyme activates stratum corneum chymotryptic enzyme through cleavage of the 31 kDa proenzyme resulting in the mature 28 kDa enzyme
-
-
?
proform filaggrin + H2O
mature filaggrin + ?
show the reaction diagram
Ser-Ser-Ser-Arg-Ile-Ile-Asn + H2O
Ser-Ser-Ser-Arg + Ile-Ile-Asn
show the reaction diagram
-
moderate cleavage efficiency
-
-
?
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
tert-butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Tos-Gly-Pro-Arg-7-amido-4-methyl-coumarin + H2O
Tos-Gly-Pro-Arg + 7-amino-4-methyl-coumarin
show the reaction diagram
-
-
-
-
?
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Vitronectin + H2O
?
show the reaction diagram
-
cleavage after Arg8
-
-
?
VPR-amido-4-methylcoumarin + H2O
VPR + 7-amino-4-methylcoumarin
show the reaction diagram
fluorogenic trypsin-like peptide substrate
-
-
?
Z-Gly-Gly-Arg-7-amido-4-methyl-coumarin + H2O
Z-Gly-Gly-Arg + 7-amino-4-methyl-coumarin
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
defensin-1alpha + H2O
?
show the reaction diagram
-
KLK5 is capable of processing defensin-1alpha and may be important in vaginal host defense
-
-
?
desmocollin-2 + H2O
?
show the reaction diagram
-
-
-
-
?
desmocollin-3 + H2O
?
show the reaction diagram
-
-
-
-
?
desmoglein 1 + H2O
?
show the reaction diagram
-
-
-
-
?
desmoglein protein 1 + H2O
?
show the reaction diagram
-
-
-
-
?
mucin 4 + H2O
?
show the reaction diagram
-
-
-
-
?
mucin 5B + H2O
?
show the reaction diagram
-
-
-
-
?
polypeptide + H2O
peptides
show the reaction diagram
proform filaggrin + H2O
mature filaggrin + ?
show the reaction diagram
-
the enzyme and profilaggrin co-localize in the stratum granulosum in human epidermis. The enzyme interacts with its substrates in granular cells
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8,8'-paepalantine dimer
-
potent competitive inhibitor
Alpha1-antitrypsin
-
-
-
alpha2-antiplasmin
-
50% inhibition at 0.8 microM in 0.6 min
-
alpha2-Macroglobulin
-
-
-
antipain
-
13% inhibition at 0.2 mM
antithrombin III
-
50% inhibition at 0.8 microM in 6 min
-
Aprotinin
-
34% inhibition at 0.1 g/ml
chymostatin
-
-
citrate
-
-
diisopropylfluorophosphate
-
87% inhibition at 2 mM
E-64
-
12% inhibition at 0.5 mM
leupeptin
lympho-epithelial Kazal type inhibitor fragment D5
-
-
-
lympho-epithelial Kazal type inhibitor fragment D6
-
-
-
lympho-epithelial Kazal type inhibitor fragment D8-D11
-
-
-
lympho-epithelial Kazal type inhibitor fragment D9-D15
-
-
-
lymphoepithelial Kazal-type 5 serine protease inhibitor
-
-
-
N-alpha-p-tosyl-L-leucine-chloromethylketone
-
14% inhibition at 0.2 mM
N-alpha-p-tosyl-L-phenylalanine-chloromethylketone
-
14% inhibition at 0.2 mM
o-phenanthroline
-
10% inhibition at 2 mM
paepalantine
-
-
SPINK9
-
vioxanthin
-
potent competitive inhibitor
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
androgens
slight up-regulation
-
citrate
-
17% activation at 120 nM, 32% activation at 1200 nM
Dermatan
-
-
estrogen
up-regulaion
heparin
-
-
progestins
up-regulaion
additional information
-
no significant effect on enzyme activity by chondroitin
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004
Abz-KLRSSKQ-EDDnp
-
pH and temperature not specified in the publication
0.05
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.13
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.11
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.61
Boc-Gln-Ala-Arg-7-amido-4-methyl-coumarin
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
1.41
Boc-Gln-Arg-Arg-7-amido-4-methyl-coumarin
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.57
Boc-Gln-Gly-Arg-7-amido-4-methyl-coumarin
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.48
Boc-Leu-Arg-Arg-7-amido-4-methyl-coumarin
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.15
Boc-Leu-GLy-Arg-7-amido-4-methylcoumarin
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
1.01
Boc-Leu-Lys-Arg-7-amido-4-methyl-coumarin
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.19
Boc-Phe-Ser-Arg-7-amido-4-methyl-coumarin
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.64
Boc-Val-Leu-Lys-7-amido-4-methyl-coumarin
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.02
Boc-Val-Pro-Arg-7-amido-4-methyl-coumarin
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.03
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.03
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
1.77
H-Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.6
L-Ile-Pro-Arg-p-nitroanilide
-
in 0.1 M Tris, 0.5 M NaCl, pH 8
1.69
Tos-Gly-Pro-Arg-7-amido-4-methyl-coumarin
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.31
Z-Gly-Gly-Arg-7-amido-4-methyl-coumarin
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.5
Abz-KLRSSKQ-EDDnp
Homo sapiens
-
pH and temperature not specified in the publication
0.05
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin
Mus musculus
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.13
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Mus musculus
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.11
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin
Mus musculus
-
in 0.1 M Tris-HCl buffer (pH 8.0)
1.78
Boc-Gln-Ala-Arg-7-amido-4-methyl-coumarin
Homo sapiens
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.41
Boc-Gln-Arg-Arg-7-amido-4-methyl-coumarin
Homo sapiens
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.18
Boc-Gln-Gly-Arg-7-amido-4-methyl-coumarin
Homo sapiens
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.31
Boc-Leu-Arg-Arg-7-amido-4-methyl-coumarin
Homo sapiens
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.07
Boc-Leu-GLy-Arg-7-amido-4-methylcoumarin
Homo sapiens
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.82
Boc-Leu-Lys-Arg-7-amido-4-methyl-coumarin
Homo sapiens
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
2.83
Boc-Phe-Ser-Arg-7-amido-4-methyl-coumarin
Homo sapiens
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.08
Boc-Val-Leu-Lys-7-amido-4-methyl-coumarin
Homo sapiens
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
3.28
Boc-Val-Pro-Arg-7-amido-4-methyl-coumarin
Homo sapiens
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.03
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin
Mus musculus
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.03
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin
Mus musculus
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.47
H-Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin
Homo sapiens
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.35
Tos-Gly-Pro-Arg-7-amido-4-methyl-coumarin
Homo sapiens
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
0.04
Z-Gly-Gly-Arg-7-amido-4-methyl-coumarin
Homo sapiens
-
100 mM phosphate, 0.01% Tween 20, pH 8.0, 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2125
Abz-KLRSSKQ-EDDnp
Homo sapiens
-
pH and temperature not specified in the publication
10357
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0244
8,8'-paepalantine dimer
-
pH and temperature not specified in the publication
0.00107
alpha2-antiplasmin
-
-
-
0.0091
antithrombin III
-
-
-
0.0017
leupeptin
-
at 25C in 0.2 M NaCl, 0.1 M Tris-HCl (pH 8.0), and 9% (w/v) Me2SO
0.0000328
lympho-epithelial Kazal type inhibitor fragment D5
-
-
-
0.0000833
lympho-epithelial Kazal type inhibitor fragment D6
-
-
-
0.0000037
lympho-epithelial Kazal type inhibitor fragment D8-11
-
-
-
0.0001187
lympho-epithelial Kazal type inhibitor fragment D9-15
-
-
-
0.0475
paepalantine
-
pH and temperature not specified in the publication
0.000065
serine protease inhibitor Kazal type 9
-
-
-
0.000257
SPINK9
-
pH 8, 21C
-
0.0229
vioxanthin
-
pH and temperature not specified in the publication
0.002 - 0.008
Zn2+
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0729
8,8'-paepalantine dimer
Homo sapiens
-
pH and temperature not specified in the publication
0.3674
paepalantine
Homo sapiens
-
pH and temperature not specified in the publication
0.0537
vioxanthin
Homo sapiens
-
pH and temperature not specified in the publication
0.009
Zn2+
Homo sapiens
-
in 150 mM NaCl, 20 mM HEPES, 0.04 mM imidazole, 0.005% (v/v) Tween 20, at pH 7.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
from ovarian cancer patients
Manually annotated by BRENDA team
-
level of KLK 5 peaks around 4 days post ovulation
Manually annotated by BRENDA team
of plantar skin
Manually annotated by BRENDA team
-
interfollicular epidermis; terminal
Manually annotated by BRENDA team
-
pre-malignant keratinocyte cell line
Manually annotated by BRENDA team
-
high expression
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
gene overexpression in a subset of more aggressive ovarian tumors
Manually annotated by BRENDA team
-
low expression
Manually annotated by BRENDA team
-
high expression
Manually annotated by BRENDA team
-
decreased immunohistochemical expression compared to normal cells
Manually annotated by BRENDA team
-
KLK5 peaks slightly at mid-cycle and shows a major peak at day 25 of menstrual cycle
Manually annotated by BRENDA team
-
female and male
Manually annotated by BRENDA team
-
expression
Manually annotated by BRENDA team
-
enzyme expression
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25500
-
non-reducing SDS-PAGE
26000
-
calculated from sequence
28000
-
recombinant deglycosylated enzyme, gel filtration
28500
-
reducing SDS-PAGE
33000
about, native and recombinant enzyme, gel filtration
40000
-
native glycosylated enzyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 25000-33000, native enzyme, SDS-PAGE; 1 * 30000-37000, recombinant enzyme, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
proteolytic modification
can be processed to active enzyme from inactive precursor by trypsin
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystallized in complex with leupeptin, sitting drop vapour diffusion method with 0.2 M ammonium sulfate, 0.1 M Mes (pH 5.6) and 30% (w/v) PEG monomethyl ether 500
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 2 months, no loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from epidermis; recombinant from CHO-K1 cells
heparin-Sepharose column chromatography and benzamidine-Sepharose column chromatography
-
Ni2+-chelate column chromatography and Resource Q column chromatography
-
recombinant as His-tagged enzyme from Escherichia coli BL21(DE3)
-
recombinant enzyme from CHO-CCL-61 cells and from Pichia pastoris
-
reversed phase chromatography
-
two chromatography steps, 95% purity
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned from a keratinocyte library, DNA sequence determination and analysis, expression in CHO-K1 cells via infection with Semliki Forest Virus system
cloned from RNA isolated from HaCaT cells, expression as His-tagged enzyme in Escherichia coli BL21(DE3)
-
DNA sequence determination and analysis, expression in CHO-CCL-61 cells, in Escherichia coli, and in Pichia pastoris
-
expressed in a baculovirus-insect cell system
-
expressed in Escherichia coli strain BL21
-
expressed in insect cell/baculovirus expression system
-
expressed in insect cells
-
expression in Pichia pastoris
-
gene KLK5, genetic organization and structure of the kallikrein gene family, clustered on chromosome 19q13.3-q13.4
gene KLK5, located at the chromosomal locus 19q13.3-q13.4, quantitative real-time PCR expression analysis, 10 transcription factors as potential regulators were found on the control regions of both KLK5 and KLK7
-
KLK5 quantitative real-time PCR expression analysis
-
overexpression in OV-MZ-6 cancer cell line inoculated into the peritoneal cavity of nude mice promotes tumor growth in mice
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
10 transcription factors as potential regulators are found on the control regions of both KLK5 and KLK7
-
expression of kallikrein 5 is higher in oral squamous cell carcinoma cell compared to pre-malignant cells
-
kallikrein 5 has increased activity levels in eczematous lesions
-
KLK5 gene expression is severely upregulated in androgen-independent prostate cancer cells after treatment with the chemotherapeutic agents docetaxel and mitoxantrone
-
the enzyme is upregulated in some types of cancers, mainly prostate and ovarian cancers
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
enzyme knockout in NHEK cells using KLK5 shRNA expressing vectors
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
medicine