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Information on EC 3.4.21.B25 - PACE4 proprotein convertase and Organism(s) Mus musculus and UniProt Accession P29121

for references in articles please use BRENDA:EC3.4.21.B25
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B25 PACE4 proprotein convertase
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This record set is specific for:
Mus musculus
UNIPROT: P29121
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
releases mature proteins from their proproteins by cleavage of Arg-Xaa-Yaa-Arg-Zaa bonds, where Xaa can be any amino acid and Yaa is Arg or Lys
Synonyms
pace4, pace-4, proprotein convertase pace4, paired basic amino acid-cleaving enzyme 4, xpace4, pace 4, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PACE-4
-
-
paired basic amino acid-cleaving enzyme 4
-
-
SPC4
-
-
subtilisin-like proprotein convertase 4
-
-
subtilisin-like proprotein convertase PACE4
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-
CAS REGISTRY NUMBER
COMMENTARY hide
151662-24-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
preprohepcidin + H2O
?
show the reaction diagram
CLIP protein + H2O
?
show the reaction diagram
-
-
-
-
?
pro-Nodal + H2O
?
show the reaction diagram
-
-
-
-
?
proform bone morphogenetic protein 6 + H2O
mature bone morphogenetic protein 6 + ?
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
preprohepcidin + H2O
?
show the reaction diagram
key role of the convertases furin, PACE4, PC5 and/or PC7 in the generation and secretion of active hepcidin
-
-
?
CLIP protein + H2O
?
show the reaction diagram
-
-
-
-
?
pro-Nodal + H2O
?
show the reaction diagram
-
-
-
-
?
proform bone morphogenetic protein 6 + H2O
mature bone morphogenetic protein 6 + ?
show the reaction diagram
-
BMP6, the substrate is exclusively found in hypertrophic chondrocytes
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha1-antitrypsin
-
-
-
decanoyl-Arg-Val-Lys-Arg-chloromethylketone
-
-
additional information
-
alpha1-proteinase inhibitor-Portland and alpha1-proteinase inhibitor-Pittsburgh have little effect on PACE4 activity
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
expression pattern of bone-related genes by RT-PCR/quantitative real-time PCR
Manually annotated by BRENDA team
-
hypertrophic chondrocyte
Manually annotated by BRENDA team
additional information
-
3T3 cell
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
the enzyme belongs to the subtilisin-like proprotein convertase (SPC) family
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PCSK4_MOUSE
655
0
73214
Swiss-Prot
Secretory Pathway (Reliability: 1)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression pattern analysis of the bone-related enzyme gene by RT-PCR/quantitative real-time PCR
-
overexpression of FL-PACE4 in COS-1 cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme PACE4 mRNA levels increase markedly during chondrogenic differentiation of ATDC-5 cells, overview
-
estrogen stimuli upregulate the enzyme expression
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PACE4 expression is markedly decreased in the presence of 0.05 mM decanoyl-L-Arg-L-Val-L-Lys-L-Arg-chloromethylketone
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PACE4 mRNA levels increase markedly during myogenesis, the PI3K inhibitor LY294002 blocks the induction of PACE4 mRNA, up-regulation of PACE4 transcripts is mediated through the PI3 kinase pathway
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the enzyme is downregulated by equol or daidzein
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mains, R.E.
Proprotein convertase PACE4
Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , Eds. ) Academic Press
2
1871-1874
2004
Aplysia sp., Mus musculus, Homo sapiens (P29122), Rattus norvegicus (Q63415)
-
Manually annotated by BRENDA team
Nour, N.; Mayer, G.; Mort, J.S.; Salvas, A.; Mbikay, M.; Morrison, C.J.; Overall, C.M.; Seidah, N.G.
The cysteine-rich domain of the secreted proprotein convertases PC5A and PACE4 functions as a cell surface anchor and interacts with tissue inhibitors of metalloproteinases
Mol. Biol. Cell
16
5215-5226
2005
Mus musculus
Manually annotated by BRENDA team
Padua, M.B.; Hansen, P.J.
Regulation of DNA synthesis and the cell cycle in human prostate cancer cells and lymphocytes by ovine uterine serpin
BMC Cell Biol.
9
5
2008
Mus musculus (P29121)
Manually annotated by BRENDA team
Yuasa, K.; Masuda, T.; Yoshikawa, C.; Nagahama, M.; Matsuda, Y.; Tsuji, A.
Subtilisin-like proprotein convertase PACE4 is required for skeletal muscle differentiation
J. Biochem.
146
407-415
2009
Mus musculus
Manually annotated by BRENDA team
Mesnard, D.; Donnison, M.; Fuerer, C.; Pfeffer, P.L.; Constam, D.B.
The microenvironment patterns the pluripotent mouse epiblast through paracrine Furin and Pace4 proteolytic activities
Genes Dev.
25
1871-1880
2011
Mus musculus
Manually annotated by BRENDA team
Yuasa, K.; Futamatsu, G.; Kawano, T.; Muroshita, M.; Kageyama, Y.; Taichi, H.; Ishikawa, H.; Nagahama, M.; Matsuda, Y.; Tsuji, A.
Subtilisin-like proprotein convertase paired basic amino acid-cleaving enzyme 4 is required for chondrogenic differentiation in ATDC5 cells
FEBS J.
279
3997-4009
2012
Mus musculus
Manually annotated by BRENDA team
Kim, H.; Tabata, A.; Tomoyasu, T.; Ueno, T.; Uchiyama, S.; Yuasa, K.; Tsuji, A.; Nagamune, H.
Estrogen stimuli promote osteoblastic differentiation via the subtilisin-like proprotein convertase PACE4 in MC3T3-E1 cells
J. Bone Miner. Metab.
33
30-39
2015
Mus musculus
Manually annotated by BRENDA team
Bessonnard, S.; Mesnard, D.; Constam, D.
PC7 and the related proteases Furin and Pace4 regulate E-cadherin function during blastocyst formation
J. Cell Biol.
210
1185-1197
2015
Mus musculus
Manually annotated by BRENDA team