Information on EC 3.4.21.B21 - trepolisin

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The expected taxonomic range for this enzyme is: Treponema denticola

EC NUMBER
COMMENTARY hide
3.4.21.B21
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
trepolisin
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
chymotrypsin-like protease, cleaves peptide bonds containing phenylalanine and proline
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cleavage of C-N-linkage
-
-
hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY hide
212763-27-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha1-antitrypsin + H2O
?
show the reaction diagram
-
-
-
?
alpha1-proteinase inhibitor + H2O
?
show the reaction diagram
-
-
-
-
?
Angiotensin + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
factor H + H2O
?
show the reaction diagram
-
cleavage yields a a factor H subfragment of approximately 50 kDa
-
-
?
Fibrinogen + H2O
?
show the reaction diagram
-
-
-
-
?
Fibronectin + H2O
?
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
IgA + H2O
?
show the reaction diagram
-
-
-
-
?
IgG + H2O
?
show the reaction diagram
-
-
-
-
?
immunogobulin G heavy chain + H2O
?
show the reaction diagram
-
weak substrate
-
?
interleukin-1 beta + H2O
?
show the reaction diagram
-
-
-
?
interleukin-1beta + H2O
?
show the reaction diagram
-
-
-
?
interleukin-6 + H2O
?
show the reaction diagram
-
-
-
?
Laminin + H2O
?
show the reaction diagram
-
-
-
-
?
N-succinyl-AAPF-4-nitroanilide + H2O
?
show the reaction diagram
synthetic substrate
-
?
N-succinyl-AAPL-4-nitroanilide + H2O
?
show the reaction diagram
-
synthetic substrate
-
?
N-succinyl-AAPN-4-nitroanilide + H2O
?
show the reaction diagram
-
synthetic substrate
-
?
N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide + H2O
N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine + 4-nitroaniline
show the reaction diagram
PrcA + H2O
PrcA1 + PrcA2
show the reaction diagram
-
-
two auxillary polypeptides
-
?
protein factor H + H2O
?
show the reaction diagram
-
-
-
-
?
serum albumin + H2O
?
show the reaction diagram
-
-
-
-
?
Substance P + H2O
?
show the reaction diagram
-
-
-
-
?
Suc-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
Suc-Ala-Ala-Pro-Phe + p-nitroanilide
show the reaction diagram
-
-
-
-
?
succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide + H2O
succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine + 4-nitroaniline
show the reaction diagram
transferrin + H2O
?
show the reaction diagram
-
-
-
-
?
tumor necrosis factor alpha + H2O
?
show the reaction diagram
-
-
-
?
Type IV collagen + H2O
?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Fibronectin + H2O
?
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
PrcA + H2O
PrcA1 + PrcA2
show the reaction diagram
-
-
two auxillary polypeptides
-
?
protein factor H + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
increases activity somewhat or has no effect
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-mercuribenzenesulfonic acid
-
98% inhibition at 0.01 mM
Bowman-Birk soybean trypsin-chymotrypsin inhibitor
-
-
-
DFP
-
inactivates
diethyl dicarbonate
-
inactivates
diisopropyl fluorophosphates
-
97% inhibition at 1 mM
EDTA
-
41% inhibition at 5 mM
N-Ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline
-
inactivates
Soybean trypsin inhibitor
-
49% inhibition at 0.01 mM
-
thiol-blocking reagent
-
-
-
Tos-Phe-CH2Cl
-
-
ZnCl2
-
46% inhibition at 1 mM
additional information
-
not inhibited by Tos-Lys-CH2Cl, alpha1-proteinase inhibitor and pepstatin
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cysteine
-
increases activity
DTT
-
increases activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
enzyme activity of diverse strains with cell-bound substrate protein factor H, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.9
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.42
-
72000 Da and 38000 Da protein, calculated from nucleic acid sequence
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
x * 72000 + x * 40000 + x * 30000, SDS-PAGE
38000
-
three bands at 72000 Da, 43000 Da and 38000 Da after boiling, SDS-PAGE
40000
x * 72000 + x * 40000 + x * 30000, SDS-PAGE
98000
-
two bands at 100000 Da and 98000 Da before boiling, SDS-PAGE
100000
180000 - 200000
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oligomeric proteins in unheated samples, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
polymer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
heating at 100°C abolishes enzyme activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nickel affinity column chromatography
-
rapidly purified by sonication, SDS-PAGE, gel filtration, pressure filtration, 101fold purified by ion-exchange chromatography followed by gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
72 KDa and 43 KDa protein
-
expressed in Escherichia coli
gene prtP, the enzyme is encoded in the prcB-prcA-prtP operon, DNA and amino acid sequence determination and analysis, sequence comparisons of different Treponema denticola strains, phylogenetic analysis, cloning in Escherichia coli strain JM109, expression of His6-tagged enzyme in Escherichia coli strain Rosetta(DE3)/pLysS
His-tagged enzyme is expressed in Escherichia coli JM109 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
PrcB is required for native expression of PrtP, PrcB is a PrtP-binding lipoprotein that stabilizes the PrtP polypeptide during localization to the outer membrane
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
heating at 100°C results in formation of three polypeptides
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
Treponema denticola exerts a pathogenic effect on periodontal tissue via local dysregulation of inflammatory cytokines by dentilisin
additional information