Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.4.21.B10 - neurosin and Organism(s) Mus musculus and UniProt Accession P15947

for references in articles please use BRENDA:EC3.4.21.B10
preliminary BRENDA-supplied EC number
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B10 neurosin
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Mus musculus
UNIPROT: P15947
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
proteolytic cleavage of polypeptides
Synonyms
kallikrein 6, neurosin, kallikrein-related peptidase 6, kallikrein-6, serine protease 9, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
kallikrein 6
kallikrein-6
-
kallikrein-related peptidase 6
-
-
protease M
-
-
S01.236
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
149565-66-2
-
9001-01-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-synuclein + H2O
?
show the reaction diagram
-
-
-
?
myelin basic protein + H2O
?
show the reaction diagram
-
close relationship between protease M and myelin proteins
-
-
?
proform protease activated receptor 1 + H2O
mature protease activated receptor 1 + ?
show the reaction diagram
-
-
-
-
?
proform protease activated receptor 2 + H2O
mature protease activated receptor 2 + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme activates G-protein coupled protease activated receptors
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-synuclein + H2O
?
show the reaction diagram
-
-
-
?
proform protease activated receptor 1 + H2O
mature protease activated receptor 1 + ?
show the reaction diagram
-
-
-
-
?
proform protease activated receptor 2 + H2O
mature protease activated receptor 2 + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme activates G-protein coupled protease activated receptors
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Aprotinin
-
pan-serine protease inhibitor
RNAi
-
suppression of protease M, causes a decrease in myelin basic protein mRNA in cultured oligodendrocytes
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
expression of protease M is increased in GST-pi-positive oligodendrocytes during remyelination. After tumor necrosis factor-alpha and IFN-gamma stimulation colocalization of protease M and myelin basic protein in the oligodendroglial process
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene KLK6, glandular kallikrein K6 precursor
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
primary cell culture
Manually annotated by BRENDA team
-
altered morphology (spindle like cell morphology) and proliferation of keratinocytes with ectopic Klk6 expression. Klk6 expression induces keratinocyte migration and invasion
Manually annotated by BRENDA team
-
under resting conditions, Neu 7 astrocytes exhibit a high basal level of stellation, protease activated receptors PAR1 and PAR2 cause a significant reversal of astrocyte stellation
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KLK1_MOUSE
261
0
28775
Swiss-Prot
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R80Q
a neurosin genetically modified for increased half-life
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in a baculovirus/insect system
-
gene encodes for multiple transcript variants
-
gene Klk6, quantitative enzyme expression analysis
-
into pPUR-tRNA plasmid and transfected into primary oligodendrocytes
-
pcDNA3.1-Klk6-Myc/His-tagged fusion protein expressed in the mouse keratinocyte cell line MCA3D
-
quantitative PCR-based enzyme expression analysis
-
the enzyme is cloned into the third generation self-inactivating lentivirus vector with the CMV promoter driving expression
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
injury-induced elevations in enzyme level
-
SJL mice infecteion with Theiler's murine encephalomyelitis virus causes an increase in enzyme expression in the spinal cord and in splenocytes. Klk6 RNA is up-regulated in splenocytes in a viral capsid protein dependent fashion, using recombinnatly expressed encodingTMEVVP1 andVP2 capsid proteins, and in THP-1 monocytes activated by phorbol 12-myristate-13-acetate and lipopolysaccharide
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Homo sapiens (Q92876), Mus musculus (P15947)
Manually annotated by BRENDA team
Yamaguchi, N.
Neurosin or human kallikrein 6
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1585-1587
2004
Homo sapiens, Mus musculus, Rattus norvegicus
-
Manually annotated by BRENDA team
Klucky, B.; Mueller, R.; Vogt, I.; Teurich, S.; Hartenstein, B.; Breuhahn, K.; Flechtenmacher, C.; Angel, P.; Hess, J.
Kallikrein 6 induces E-cadherin shedding and promotes cell proliferation, migration, and invasion
Cancer Res.
67
8198-8206
2007
Homo sapiens (Q92876), Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Bando, Y.; Ito, S.; Nagai, Y.; Terayama, R.; Kishibe, M.; Jiang, Y.P.; Mitrovic, B.; Takahashi, T.; Yoshida, S.
Implications of protease M/neurosin in myelination during experimental demyelination and remyelination
Neurosci. Lett.
405
175-180
2006
Mus musculus, Mus musculus BALB/c
Manually annotated by BRENDA team
Pampalakis, G.; Arampatzidou, M.; Amoutzias, G.; Kossida, S.; Sotiropoulou, G.
Identification and analysis of mammalian KLK6 orthologue genes for prediction of physiological substrates
Comput. Biol. Chem.
32
111-121
2008
Bos taurus (A6QQ95), Bos taurus, Canis lupus familiaris, Homo sapiens (Q92876), Homo sapiens, Macaca mulatta, Mus musculus, Pan troglodytes, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Scarisbrick, I.A.; Epstein, B.; Cloud, B.A.; Yoon, H.; Wu, J.; Renner, D.N.; Blaber, S.I.; Blaber, M.; Vandell, A.G.; Bryson, A.L.
Functional role of kallikrein 6 in regulating immune cell survival
PLoS ONE
6
e18376
2011
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Scarisbrick, I.A.; Radulovic, M.; Burda, J.E.; Larson, N.; Blaber, S.I.; Giannini, C.; Blaber, M.; Vandell, A.G.
Kallikrein 6 is a novel molecular trigger of reactive astrogliosis
Biol. Chem.
393
355-367
2012
Mus musculus
Manually annotated by BRENDA team
Scarisbrick, I.A.; Yoon, H.; Panos, M.; Larson, N.; Blaber, S.I.; Blaber, M.; Rodriguez, M.
Kallikrein 6 regulates early CNS demyelination in a viral model of multiple sclerosis
Brain Pathol.
22
709-722
2012
Homo sapiens (Q92876), Mus musculus, Mus musculus SJL/J
Manually annotated by BRENDA team
Yoon, H.; Radulovic, M.; Wu, J.; Blaber, S.I.; Blaber, M.; Fehlings, M.G.; Scarisbrick, I.A.
Kallikrein 6 signals through PAR1 and PAR2 to promote neuron injury and exacerbate glutamate neurotoxicity
J. Neurochem.
127
283-298
2013
Mus musculus, Mus musculus C57/BL6J
Manually annotated by BRENDA team
Yoon, H.; Scarisbrick, I.A.
Kallikrein-related peptidase 6 exacerbates disease in an autoimmune model of multiple sclerosis
Biol. Chem.
397
1277-1286
2016
Mus musculus
Manually annotated by BRENDA team
Yoon, H.; Radulovic, M.; Scarisbrick, I.A.
Kallikrein-related peptidase 6 orchestrates astrocyte form and function through proteinase activated receptor-dependent mechanisms
Biol. Chem.
399
1041-1052
2018
Mus musculus
Manually annotated by BRENDA team
Spencer, B.; Valera, E.; Rockenstein, E.; Trejo-Morales, M.; Adame, A.; Masliah, E.
A brain-targeted, modified neurosin (kallikrein-6) reduces alpha-synuclein accumulation in a mouse model of multiple system atrophy
Mol. Neurodegener.
10
48
2015
Mus musculus (Q91Y82), Mus musculus
Manually annotated by BRENDA team
Kishibe, M.; Baida, G.; Bhalla, P.; Lavker, R.M.; Schlosser, B.; Iinuma, S.; Yoshida, S.; Dudley, J.T.; Budunova, I.
Important role of kallikrein 6 for the development of keratinocyte proliferative resistance to topical glucocorticoids
Oncotarget
7
69479-69488
2016
Mus musculus
Manually annotated by BRENDA team