Information on EC 3.4.21.99 - spermosin

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The expected taxonomic range for this enzyme is: Halocynthia roretzi

EC NUMBER
COMMENTARY hide
3.4.21.99
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RECOMMENDED NAME
GeneOntology No.
spermosin
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolyses arginyl bonds, preferably with Pro in the P2 position
show the reaction diagram
the enzyme from the ascidian -Prochordate- Halocynthia roretzi is localised in the sperm head, and released during sperm activation. A proline-rich region is involved in binding to the vitelline coat of the egg. Belongs in peptidase family S1, trypsin family
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY hide
89925-67-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide + H2O
tert-butyloxycarbonyl-Val-Pro + Arg-4-methylcoumarin-7-amide
show the reaction diagram
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?
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide + H2O
tert-butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
not strongly influenced by changing salt concentrations except Ca2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-pyrrolidone-2-carboxyl-Leu-DL-arginal
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0.01 mM, 25% inhibition
Aprotinin
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weak
benzamidine
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benzyloxycarbonyl-Val-Pro-Arg-H
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dansyl-Leu-DL-arginal
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0.01 mM, 24% inhibition
dansyl-Val-Pro-Arg-H
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diisopropyl fluorophosphate
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Gly-Val-Arg-CH2Cl
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Hg2+
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1 mM, strong inhibition
leupeptin
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weak
N-acetyl-Leu-Leu-arginal
N-benzoyl-Leu-arginal
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0.01 mM, 15% inhibition
N-benzyloxycarbonyl-Phe-Leu-arginal
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0.01 mM, 93% inhibition
N-benzyloxycarbonyl-Val-Pro-arginal
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0.01 mM, 99% inhibition
o-nitrophenyl-Leu-arginal
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0.01 mM, 52% inhibition
ovomucoid
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-
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p-aminobenzamidine
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p-nitrobenzyl-Leu-arginal
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0.01 mM, 26% inhibition
peptidyl-Arg-H
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phenylmethanesulfonyl fluoride
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Pro-Leu-arginal
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0.01 mM, 27% inhibition
Soybean trypsin inhibitor
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Val-Pro-Arg-CH2Cl
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Zn2+
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1 mM, strong inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.145
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
no activity detected in hepatopancreas, intestine and brachial basket
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
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gel filtration, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 27000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
bovine serum albumin stabilizes
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non-ionic detergents such as Brij 35, Triton X-100, Tween 80 at concentrations between 0.005% and 0.1% stabilize
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stabilized by nonionic detergents (Brij 35, Tween 80 or Triton X-100) at 0.005%
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stable in presence of contaminating proteins, very labile in their absence
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE