Information on EC 3.4.21.83 - Oligopeptidase B

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.4.21.83
-
RECOMMENDED NAME
GeneOntology No.
Oligopeptidase B
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Hydrolysis of -Arg-/-, -Lys-/- bonds in oligopeptides, even when P1' residue is proline
show the reaction diagram
hydrolysis of Arg-+-, Lys-+- bonds in oligopeptides, even when P1' residue is proline, the term-+- depicts the point of cleavage
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
La_OpB
A7XAB0
-
oligopeptidase B
-
-
oligopeptidase B
A7XAB0
-
oligopeptidase B
B3VI58
-
oligopeptidase B
Serratia proteamaculans 94
B3VI58
-
-
oligopeptidase B
-
-
oligopeptidase B
-
-
oligopeptidase B
-
-
oligopeptidase B
-
-
OpdB
Q9L6C8
-
OpdB
Serratia proteamaculans 94
B3VI58
-
-
Protease II
-
-
-
-
Proteinase, Escherichia coli alkaline, II
-
-
-
-
PSP
Serratia proteamaculans 94
B3VI58
-
-
trypsin-like protease
B3VI58
-
trypsin-like protease
Serratia proteamaculans 94
B3VI58
-
-
CAS REGISTRY NUMBER
COMMENTARY
57657-67-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Escherichia coli JM83 harboring pPR0II-12
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-
Manually annotated by BRENDA team
HB101 gene cloned and expressed in JM83
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-
Manually annotated by BRENDA team
strain ATCC 11303
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-
Manually annotated by BRENDA team
-
A7XAB0
SwissProt
Manually annotated by BRENDA team
serovar typhimurium, virulent wild-type strain SL1344
SwissProt
Manually annotated by BRENDA team
Serratia proteamaculans 94
strain 94
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
C9EF60
knock-out of OPB results in the disappearance of the serine protease activity of Leishmania extracts. OPB null parasites show an elevated content of enolase protein. This enolase is enzymatically inactive and associated with the parasite membrane. OPB deletion results in a striking alteration in macrophage responses to Leishmania. Whereas wild type parasites elicited little, if any, response from infected macrophages, OPB deletion parasites induce a massive up-regulation in gene transcription. These OPB deletion parasites display decreased virulence in the murine footpad indection model
physiological function
-
oligopeptidase B null mutant parasites grow at a significantly faster rate in vitro, and are as virulent as wild type strains during infection in mice. Parasites are revealed in extra vascular regions showing that OPB is not involved in assisting Trypanosoma brucei parasites to cross microvascular endothelial cells. Null mutants show an increase in discrete cysteine peptidase activities when compared to wild type strains. A significant increase of prolyl oligopeptidase activity is observed in mutant, but no concomitant increase in prolyl oligopeptidase protein levels
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-ADGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ADGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AEGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AEGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AFGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AFGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGDRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGDR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGERGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGER + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGFRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGFR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRDAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + DAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGREAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + EAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRFAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + FAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGADQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GADQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRGAEQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAEQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAFQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAFQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAHQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAHQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
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?
2-aminobenzoyl-AGGRGAIQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAIQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRGALQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GALQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAMQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAMQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGANQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GANQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAPQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAQQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAQQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGASQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GASQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGATQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GATQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAVQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAVQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAYQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAYQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGDGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GDGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGEGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GEGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGFGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GFGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGGGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GGGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGHGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GHGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGIGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GIGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGLGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GLGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGMGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GMGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGNGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GNGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGPGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GPGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGQGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GQGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGSGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGRG + Ser-Gly-Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRGTGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GTGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGVGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GVGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGYGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GYGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRHAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + HAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRIAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + IAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRLAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + LAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRMAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + MAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRNAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + NAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRPAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + PAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRQAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + QAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRSAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + SAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRTAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + TAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRVAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + VAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRYAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + YAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGHRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGHR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGIRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGIR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGKRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGKR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGLRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGLR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGMRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGMR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGNRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGNR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGPRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGPR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGQRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGQR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGRRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGRR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGRRRAQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGSRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGSR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGTRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGTR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGVRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGVR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGYRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGYR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AHGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AHGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AHKRYSHQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AIGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AIGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-ALGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ALGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AMGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AMGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AMRRTISQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
-
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?
2-aminobenzoyl-ANGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ANGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
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?
2-aminobenzoyl-APGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-AQGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AQGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-ASGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ASGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-ATGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ATGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-AVGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AVGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-AYGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AYGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-FRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
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?
2-aminobenzoyl-FRSSR-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-Phe-Arg + Ser-Ser-Arg-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-GFSPFRSSRIGIKEEQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-GFSPFR + SSRIGIKEEQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-GFSPFRSSRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
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-
-
?
2-aminobenzoyl-LGMISLMKRPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-LGMISLMK + Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-MISLMKRPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-MISLMK + Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-RPPGFSPFRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-RPPGFSPFR + Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-RPPGFSPFRSSRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-RPPGFSPFR + Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-Thr-Arg Cit-Phe(NO2)-Ser-Leu-NH2 + H2O
?
show the reaction diagram
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?
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 + H2O
?
show the reaction diagram
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-
-
?
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2 + H2O
?
show the reaction diagram
-
-
-
?
2-aminobenzoyl-Thr-Cit-Cit-Phe(NO2)-Ser-Leu-NH2 + H2O
?
show the reaction diagram
-
-
-
?
3-(2-furyl)acryloyl-Tyr-Lys-Arg + H2O
3-(2-furyl)acryloyl-Tyr-Lys + Arg
show the reaction diagram
Q6QDP1, -
-
-
-
?
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide + H2O
acetyl-L-Leu-L-Leu-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide + H2O
acetyl-L-Leu-L-Lys-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Acetyl-Tyr 4-nitroanilide + H2O
Acetyl-Tyr + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
adrenocorticotropic hormone + H2O
SYSMEHFR + WGK + WGKPVGK + VYP
show the reaction diagram
-
-
-
-
?
Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
Arg-Arg-2-naphthylamide + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
Arg-Arg-Arg-OH + H2O
Arg-Arg + Arg
show the reaction diagram
Q6QDP1, -
-
-
-
?
Atrial natriuretic factor + H2O
?
show the reaction diagram
Q6QDP1, -
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi
-
-
?
Atrial natriuretic factor + H2O
?
show the reaction diagram
Q6QDP1, -
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor
-
-
?
azocasein + H2O
?
show the reaction diagram
-
-
-
-
-
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
Benzoyl-Arg 2-naphthylamide + H2O
Benzoyl-Arg + 2-naphthylamine
show the reaction diagram
-
-
-
-
-
benzoyl-Arg-2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
?
benzoyl-Arg-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
benzoyl-Arg-beta-naphthylamide + H2O
?
show the reaction diagram
-
-
-
?
benzoyl-Arg-Et + H2O
?
show the reaction diagram
-
-
-
?
benzoyl-Arg-Et + H2O
?
show the reaction diagram
-
-
-
?
benzoyl-Arg-NH2 + H2O
?
show the reaction diagram
-
-
-
-
-
benzoyl-Arg-NH2 + H2O
?
show the reaction diagram
-
-
-
?
benzoyl-Arg-NH2 + H2O
?
show the reaction diagram
-
-
-
?
benzoyl-arginine ethyl ester + H2O
?
show the reaction diagram
-
-
-
?
benzoyl-citrulline-Et + H2O
?
show the reaction diagram
-
-
-
?
benzoyl-citrulline-Et + H2O
?
show the reaction diagram
-
-
-
?
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Gly-Gly-L-Arg + 7-amino-4-methylcoumarin S: more |
show the reaction diagram
C9EF60, -
-
-
-
?
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
C9EF60, -
-
-
-
?
benzoyl-L-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzoyl-L-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-, Q4QHU7
-
-
-
?
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O
benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Benzoyl-Lys 2-naphthylamide + H2O
Benzoyl-Lys + 2-naphthylamine
show the reaction diagram
-
-
-
-
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
benzyloxycarbonyl-Arg-Arg-7-(4-methylcoumaryl)amide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Gly-L-Arg-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-, Q4QHU7
-
-
-
?
benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-Leu-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Leu-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Val-L-Val-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amino-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
Boc-Leu-Lys-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
CBZ-L-Arg-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
weak substrate
-
-
?
Denatured casein + H2O
?
show the reaction diagram
-
weak
-
-
-
DL-Val-Leu-Arg-paranitroanilide + H2O
DL-Val-Leu-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Glucagon + H2O
?
show the reaction diagram
-
wild type enzyme cleaves Lys12-Tyr13, Arg17-Arg18, and Arg18-Ala19 bonds of glucagon
-
-
?
Gly-Arg-2-naphthylamide + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
Gly-Gly-Lys-Arg + H2O
Gly-Gly-Lys-Arg + Arg
show the reaction diagram
Q6QDP1, -
-
-
-
?
histone + H2O
?
show the reaction diagram
Q9L6C8, -
human histones H1, H2A, H3 and H4, restricted proteolysis
-
?
Insulin B-chain + H2O
?
show the reaction diagram
-
cleaves the carboxymethylated B-chain of insulin at the Arg22-Gly23 bond, but after prolonged periods of incubation it is also able to cleave the Tyr16-Leu17 bond
-
-
-
Insulin B-chain + H2O
?
show the reaction diagram
-
cleavage at the carboxyl sides of Arg and Lys residues
-
-
-
L-pGlu-Gly-L-Arg-7-amido-4-methylcoumarin + H2O
L-pGlu-Gly-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low hydrolytic rate
-
-
?
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin + H2O
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low hydrolytic rate
-
-
?
L-Phe-L-Arg 2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
-
N-Benzoyl-DL-Arg 4-nitroanilide + H2O
N-Benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
N-Benzoyl-L-Arg ethyl ester + H2O
N-Benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
-
-
-
N-benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
N-Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O
N-Benzyloxycarbonyl-Gly + 4-nitrophenol
show the reaction diagram
-
weak
-
-
-
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Gly-Gly-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-, Q4QHU7
-
-
-
?
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-, Q4QHU7
-
-
-
?
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-, Q4QHU7
-
-
-
?
N-Benzyloxycarbonyl-Lys benzyl ester + H2O
N-Benzyloxycarbonyl-Lys + benzol
show the reaction diagram
-
-
-
-
-
N-Carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O
N-Carbobenzoxy-L-Lys + 4-nitrophenol
show the reaction diagram
-
-
-
-
-
N-tosyl-Gly-Pro-Arg-paranitroanilide + H2O
N-tosyl-Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
68% of the activity with carbobenzoxy-Arg-Arg-4-methylcoumaryl-7-amide
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
B3VI58, -
-
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
Serratia proteamaculans 94
B3VI58
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Ala-L-Ala-L-Arg-4-nitroanilide + H2O
Nalpha-benzyloxycarbonyl-L-Ala-L-Ala-L-Arg + 4-nitroaniline
show the reaction diagram
-
poor substrate
-
-
?
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester + H2O
Nalpha-benzyloxycarbonyl-L-lysine + phenylmethanethiol
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide + H2O
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Phe-Arg-2-naphthylamide + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
Phe-Arg-Arg + H2O
Phe-Arg + Arg
show the reaction diagram
Q6QDP1, -
-
-
-
?
succinyl-Gly-L-Pro-L-Lys-4-nitroanilide + H2O
succinyl-Gly-L-Pro-L-Lys + 4-nitroaniline
show the reaction diagram
-
poor substrate
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
best substrate, the hydrolytic activity is observed only in the presence of NaCl and the thiol-reacting reagents dithiothreitol, reduced glutathione, and oxidized glutathione at pH 8.5 (50 mM Tris-HCl)
-
-
?
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
tert-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
specific substrate for Escherichia coli oligopeptidase B, 20% of the activity with carbobenzoxy-Arg-Arg-4-methylcoumaryl-7-amide
-
-
?
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Q6QDP1, -
-
-
-
?
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Q9L6C8, -
-
-
?
Tosyl-Arg methyl ester + H2O
Tosyl-Arg + methanol
show the reaction diagram
-
-
-
-
-
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
tosyl-Gly-L-Pro-L-Arg + 7-amido-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
tosyl-Gly-L-Pro-L-Lys-7-amido-4-methylcoumarin + H2O
tosyl-Gly-L-Pro-L-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Tosyl-Lys methyl ester + H2O
Tosyl-Lys + benzyl alcohol
show the reaction diagram
-
-
-
-
-
Val-Lys-Arg-Arg + H2O
Val-Lys-Arg + Arg
show the reaction diagram
Q6QDP1, -
-
-
-
?
L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
L-Pro-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
not: luteinizing hormone releasing factor
-
-
-
additional information
?
-
Q9L6C8, -
exhibits amidolytic activity exclusively against substrates with basic residues in P1, no activity is observed against H-Arg-4-methylcoumarin 7-amide, H-Leu-4-methylcoumarin 7-amide, H-Lys-4-methylcoumarin 7-amide, benzyloxycarbonyl-Leu-Leu-Glu-beta-nitroanilide, benzyloxycarbonyl-Gly-Gly-Leu-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-4-methylcoumarin 7-amide, Suc-Ile-Ala-4-methylcoumarin 7-amide, Suc-Gly-Pro-4-methylcoumarin 7-amide, Ac-Tyr-Val-Ala-Asp-p-nitroanilide, Suc-Ala-Ala-Pro-Phe-4-methylcoumarin 7-amide
-
?
additional information
?
-
-
hydrolyzes peptides with dibasic sites much faster than monobasic substrates, hydrolyzes the peptide bond at lysine and arginine residues
-
?
additional information
?
-
-
no hydrolysis of 2-aminobenzoyl acid-LGMISLMKRPPGFSPFRSSRI-NH2-N-(2,4-dinitrophenyl)ethylenediamine
-
?
additional information
?
-
-
specific for substrates with a pair of basic residues at positions P1 and P2, involved in host cell invasion
-
?
additional information
?
-
-
the enzyme may be associated with Chagas disease pathogenesis nb yhydrolyzing host proteins and inducing host immune responses
-
-
-
additional information
?
-
P24555
cleaves oxidised insulin B chain, OpdB does not hydrolyse protein substrates with the exception of nominal digestion of the endogenous Escherichia coli enzymes aspartokinase L-homoserine dehydrogenase and aspartokinase III
-
-
-
additional information
?
-
-
no hydrolytic activity against the aminopeptidase substrates (4-nitroanilide derivatives of amino acids) and FITC-casein, substrates containing Pro at Xaa positions in the FRETS library are not hydrolyzed
-
-
-
additional information
?
-
-, Q4QHU7
no substrate: N-benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin
-
-
-
additional information
?
-
C9EF60, -
OPB has a strong preference for lysine or arginine residues at P1. OPB can accommodate several amino acids in the P2P4 positions, with a preference for glycine in P2, serine, glycine, alanine, asparagine, proline, and threonine in P3, and proline in P4. Bulky hydrophobic groups, such as tyrosine, phenylalanine, and tryptophan, are least preferred
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Atrial natriuretic factor + H2O
?
show the reaction diagram
Q6QDP1, -
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi
-
-
?
additional information
?
-
-
involved in host cell invasion
-
?
additional information
?
-
-
the enzyme may be associated with Chagas disease pathogenesis nb yhydrolyzing host proteins and inducing host immune responses
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
stimulates amidase and proteolytic activities, maximal activation of 25% at concentrations above 25 mM
Ca2+
-
activation at 5-10 mM CaCl2
Ca2+
-
in presence of Ca2+, efficiency of hydrolysis of substrates with positively charged P2 residues decreases by an order of magnitude due to the worsening in the substrate binding. In the presence of 50 mM Ca2+, no substrate inhibition is observed in the case of acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide, and the effect was less pronounced for other substrates
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3,4-Dichloroisocoumarin
Q9L6C8, -
irreversible inhibition
3,4-Dichloroisocoumarin
Q6QDP1, -
irreversible
3,4-Dichloroisocoumarin
Q382P7
non-peptide irreversible serine-peptidase inhibitor
4-(2-aminoethyl)benzenesulfonyl fluoride
Q9L6C8, -
irreversible inhibition
4-(2-aminoethyl)benzenesulfonyl fluoride
Q6QDP1, -
irreversible
4-(2-aminoethyl)benzenesulfonyl fluoride
-
OpdB activity toward tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin hydrolysis is inhibited completely by 4 mM pefbloc SC
4-(2-aminoethyl)benzenesulfonylfluoride
Q382P7
non-peptide irreversible serine-peptidase inhibitor
4-methylumbelliferyl-p-guanidobenzoate
Q382P7
active site titrant
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
-
-
acetyl-Thr-Arg-Arg
-
-
agmatine
Q6QDP1, -
reversible
antipain
Q9L6C8, -
reversible inhibition
antipain
Q6QDP1, -
reversible
antipain
Q382P7
reversible competitive inhibitor
antipain
-
OpdB activity toward tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin hydrolysis is inhibited completely by 0.3 mM antipain
antipain
-, Q4QHU7
-
antipain
C9EF60
0.1 mM, no residual activity
antipain dihydrochloride
-
1 mM, 79% remaining activity
Aromatic amidines
-
-
-
Benzamidine
Q9L6C8, -
reversible inhibition
Benzamidine
Q6QDP1, -
reversible
Benzamidine
C9EF60
1 mM, 27% residual activity
benzoyl-Arg-4-nitroanilide
-
enzyme is inhibited by high concentrations of substrate
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
-
-
Ca2+
C9EF60
0.01 mM, 69% residual activity
chymostatin
-
0,1 mM, 45% inhibition
diisopropylfluorophosphate
Q382P7
non-peptide irreversible serine-peptidase inhibitor
EDTA
-
1.3 mM, 98% remaining activity, 20 mM, 57% remaining activity
Fe2+
-
-
-
iodoacetamide
-
-
iodoacetic acid
Q382P7
-
Leupeptin
-
weak
Leupeptin
Q9L6C8, -
reversible inhibition
Leupeptin
Q6QDP1, -
reversible
Leupeptin
Q382P7
reversible competitive inhibitor
Leupeptin
-
OpdB activity toward tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin hydrolysis is inhibited completely by 0.05 mM leupeptin
Leupeptin
C9EF60
0.1 mM, no residual activity
methanol
B3VI58, -
incubation in 20% (v/v) methanol (10-20 h, 4C) results in partial decomposition of the high molecular weight PSP-GroEL complex with precipitation of denatured chaperonin and a small (no more than 10%) loss of PSP activity
Mg2+
C9EF60
0.01 mM, 73% residual activity
N-ethylmaleimide
Q382P7
-
N-ethylmaleimide
-
-
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
-
-
NEM
-
Cys256 is the reactive cysteine residue that mediates OpdB inhibition. Cys256 adducts occlude the P1 substrate-binding site, preventing substrate binding
p-aminobenzamidine
-
-
p-aminobenzamidine
Q9L6C8, -
reversible inhibition
p-chloromercuribenzoate
Q382P7
non-peptide irreversible serine-peptidase inhibitor
Pefabloc SC
-
4 mM, 93% inhibition
Pefabloc SC
C9EF60
2 mM, no residual activity
Pentamidine
Q382P7
reversible competitive inhibitor
Pepstatin
-
1 microM, 94% remaining activity
peptidyl chloromethyl ketone
Q382P7
irreversible peptidyl inhibitors
peptidyl diazomethyl ketone
Q382P7
irreversible peptidyl inhibitors
peptidyl phosphonate alpha-aminoalkyl diphenyl ester
Q382P7
irreversible peptidyl inhibitors
Phe-Pro-Arg-chloromethylketone
C9EF60
0.01 mM, no residual activity
phenylmethane sulfonyl fluoride
Q9L6C8, -
irreversible inhibition
phenylmethanesulfonyl fluoride
Q6QDP1, -
irreversible
phosphoramidon
-
0.57 mM, 96% remaining activity
protamine
Q382P7
reversible competitive inhibitor
protamines
P24555
protamines, basic 30-32 residue peptides that are rich in Arg residues, are potent inhibitors of OpdB
-
suramin
Q382P7
partial non-competitive inhibitor
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin
-
in the case of added dithiothreitol, reduced glutathione, or oxidized glutathione, a high concentration of the substrate tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin inhibits the enzyme activity. By contrast, addition of 1 M NaCl to either dithiothreitol, reduced glutathione, or oxidized glutathione or 1 M NaCl alone prevents substrate inhibition
Tos-LysCH2Cl
Q382P7
non-peptide irreversible serine-peptidase inhibitor
tosyl-L-Lys-chloromethylketone
C9EF60
0.1 mM, no residual activity
tosyl-Leu chloromethyl ketone
-
-
trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester
-
IC50: about 0.01 mM. Oligopeptidase B is much more sensitive than oligopeptidase B
Zn2+
C9EF60
0.01 mM, no residual activity
Mn2+
C9EF60
0.01 mM, 73% residual activity
additional information
-
1,10-phenanthroline; EDTA; not: chelating agents; PMSF; several natural trypsin inhibitors; sulfhydryl agents
-
additional information
-
several natural trypsin inhibitors
-
additional information
Q9L6C8, -
no inhibition of wild-type enzyme with iodoacetamide, iodoacetate, N-ethylmaleimide, EDTA, EGTA, bestatin,amastatin, arphamenine A, elastinal, dynorphin A or dynorphin B
-
additional information
P24555
no inhibition by N-ethylmaleimide and iodoacetic acid. This is consistent with the absence of a cysteine residue at position 256 which is replaced by His
-
additional information
-
OpdB is not inhibited by E-64 (0.03 mM), pepstain (0.04 mM), EDTA (1 mM), chimostatin (0.2 mM), and phosphoramidon (0.4 mM)
-
additional information
-
reducing reagent such as dithiothreitol has no effect on the inactivation of wild type and mutant OPBs by heat and urea treatment
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Aprotinin
-
0.3 microM, 103% activity
bestatin
-
0.13 mM, 105% activity
cysteine
Q382P7
-
dithiothreitol
Q382P7
-
dithiothreitol
-
enzymatic activity is markedly enhanced by dithiothreitol at submillimolar level, which affects the P1 preference of OpdB synergistically in a substrate-dependent manner
E-64
-
5 microM, 114% activity
glutathione
Q382P7
-
Leupeptin
-
1 microM, 116% activity
NaCl
-
enzymatic activity is markedly enhanced by high NaCl concentration, NaCl affects the P1 preference of OpdB synergistically in a substrate-dependent manner
oxidized glutathione
-
oxidized glutathione enhances OpdB activity and affects the P1 preference of OpdB synergistically in a substrate-dependent manner
reduced glutathione
-
enzymatic activity is markedly enhanced by reduced glutathione at submillimolar level which affects the P1 preference of OpdB synergistically in a substrate-dependent manner
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0036
-
2-aminobenzoyl-ADGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.039
-
2-aminobenzoyl-AEGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0032
-
2-aminobenzoyl-AFGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0034
-
2-aminobenzoyl-AFGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.013
-
2-aminobenzoyl-AGDRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0033
-
2-aminobenzoyl-AGERGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0024
-
2-aminobenzoyl-AGFRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0031
-
2-aminobenzoyl-AGFRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0015
-
2-aminobenzoyl-AGGRDAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0045
-
2-aminobenzoyl-AGGRDAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0014
-
2-aminobenzoyl-AGGREAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0065
-
2-aminobenzoyl-AGGREAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0003
-
2-aminobenzoyl-AGGRFAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0027
-
2-aminobenzoyl-AGGRFAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0057
-
2-aminobenzoyl-AGGRGADQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.021
-
2-aminobenzoyl-AGGRGAEQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0062
-
2-aminobenzoyl-AGGRGAFQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0097
-
2-aminobenzoyl-AGGRGAFQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0011
-
2-aminobenzoyl-AGGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0072
-
2-aminobenzoyl-AGGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0034
-
2-aminobenzoyl-AGGRGAHQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0037
-
2-aminobenzoyl-AGGRGAHQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.011
-
2-aminobenzoyl-AGGRGAIQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.031
-
2-aminobenzoyl-AGGRGAIQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0073
-
2-aminobenzoyl-AGGRGALQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.012
-
2-aminobenzoyl-AGGRGALQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.003
-
2-aminobenzoyl-AGGRGAMQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0031
-
2-aminobenzoyl-AGGRGAMQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0059
-
2-aminobenzoyl-AGGRGANQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.018
-
2-aminobenzoyl-AGGRGANQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0011
-
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0028
-
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0072
-
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.013
-
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0048
-
2-aminobenzoyl-AGGRGAQQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0088
-
2-aminobenzoyl-AGGRGAQQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.002
-
2-aminobenzoyl-AGGRGASQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.012
-
2-aminobenzoyl-AGGRGASQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.006
-
2-aminobenzoyl-AGGRGATQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.027
-
2-aminobenzoyl-AGGRGATQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0091
-
2-aminobenzoyl-AGGRGAVQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.021
-
2-aminobenzoyl-AGGRGAVQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0057
-
2-aminobenzoyl-AGGRGAYQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.011
-
2-aminobenzoyl-AGGRGAYQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.008
-
2-aminobenzoyl-AGGRGDGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.012
-
2-aminobenzoyl-AGGRGDGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.01
-
2-aminobenzoyl-AGGRGEGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.138
-
2-aminobenzoyl-AGGRGEGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0021
-
2-aminobenzoyl-AGGRGFGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.012
-
2-aminobenzoyl-AGGRGFGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0062
-
2-aminobenzoyl-AGGRGGGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.014
-
2-aminobenzoyl-AGGRGGGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0028
-
2-aminobenzoyl-AGGRGHGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0033
-
2-aminobenzoyl-AGGRGHGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0032
-
2-aminobenzoyl-AGGRGIGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.011
-
2-aminobenzoyl-AGGRGIGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0052
-
2-aminobenzoyl-AGGRGLGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.024
-
2-aminobenzoyl-AGGRGLGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.003
-
2-aminobenzoyl-AGGRGMGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.02
-
2-aminobenzoyl-AGGRGMGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0046
-
2-aminobenzoyl-AGGRGNGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0092
-
2-aminobenzoyl-AGGRGNGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0012
-
2-aminobenzoyl-AGGRGPGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0098
-
2-aminobenzoyl-AGGRGPGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0023
-
2-aminobenzoyl-AGGRGQGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.01
-
2-aminobenzoyl-AGGRGQGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0032
-
2-aminobenzoyl-AGGRGSGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0037
-
2-aminobenzoyl-AGGRGSGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0058
-
2-aminobenzoyl-AGGRGTGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.013
-
2-aminobenzoyl-AGGRGTGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.02
-
2-aminobenzoyl-AGGRGVGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.031
-
2-aminobenzoyl-AGGRGVGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0039
-
2-aminobenzoyl-AGGRGYGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0044
-
2-aminobenzoyl-AGGRGYGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0005
-
2-aminobenzoyl-AGGRHAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0017
-
2-aminobenzoyl-AGGRHAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0003
-
2-aminobenzoyl-AGGRIAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0005
-
2-aminobenzoyl-AGGRIAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0006
-
2-aminobenzoyl-AGGRLAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0013
-
2-aminobenzoyl-AGGRLAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0069
-
2-aminobenzoyl-AGGRMAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0083
-
2-aminobenzoyl-AGGRMAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0004
-
2-aminobenzoyl-AGGRNAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0013
-
2-aminobenzoyl-AGGRNAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0005
-
2-aminobenzoyl-AGGRQAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0007
-
2-aminobenzoyl-AGGRQAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0005
-
2-aminobenzoyl-AGGRSAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0012
-
2-aminobenzoyl-AGGRSAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0003
-
2-aminobenzoyl-AGGRTAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0009
-
2-aminobenzoyl-AGGRTAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0001
-
2-aminobenzoyl-AGGRVAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0014
-
2-aminobenzoyl-AGGRVAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0004
-
2-aminobenzoyl-AGGRYAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0006
-
2-aminobenzoyl-AGGRYAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0063
-
2-aminobenzoyl-AGHRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0012
-
2-aminobenzoyl-AGIRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.01
-
2-aminobenzoyl-AGIRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0018
-
2-aminobenzoyl-AGKRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0048
-
2-aminobenzoyl-AGKRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0019
-
2-aminobenzoyl-AGLRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0077
-
2-aminobenzoyl-AGLRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0025
-
2-aminobenzoyl-AGMRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0029
-
2-aminobenzoyl-AGMRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.004
-
2-aminobenzoyl-AGNRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.023
-
2-aminobenzoyl-AGNRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0082
-
2-aminobenzoyl-AGPRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.016
-
2-aminobenzoyl-AGPRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0014
-
2-aminobenzoyl-AGQRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0059
-
2-aminobenzoyl-AGQRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0004
-
2-aminobenzoyl-AGSRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0006
-
2-aminobenzoyl-AGSRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0019
-
2-aminobenzoyl-AGTRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0075
-
2-aminobenzoyl-AGTRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0014
-
2-aminobenzoyl-AGVRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0039
-
2-aminobenzoyl-AGVRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0077
-
2-aminobenzoyl-AGYRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.014
-
2-aminobenzoyl-AGYRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0032
-
2-aminobenzoyl-AHGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0035
-
2-aminobenzoyl-AHGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.002
-
2-aminobenzoyl-AIGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0044
-
2-aminobenzoyl-AIGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0013
-
2-aminobenzoyl-ALGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0026
-
2-aminobenzoyl-ALGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0009
-
2-aminobenzoyl-AMGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH8.0, 37C
0.0059
-
2-aminobenzoyl-AMGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0083
-
2-aminobenzoyl-ANGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.001
-
2-aminobenzoyl-APGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH8.0, 37C
0.0019
-
2-aminobenzoyl-APGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0019
-
2-aminobenzoyl-AQGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0024
-
2-aminobenzoyl-AQGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0023
-
2-aminobenzoyl-ASGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0074
-
2-aminobenzoyl-ASGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0018
-
2-aminobenzoyl-ATGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0082
-
2-aminobenzoyl-ATGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0026
-
2-aminobenzoyl-AVGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.013
-
2-aminobenzoyl-AVGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0018
-
2-aminobenzoyl-AYGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.003
-
2-aminobenzoyl-AYGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0298
-
2-aminobenzoyl-Thr-Arg Cit-Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C
0.0048
-
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C, 3 mM
0.014
-
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 15C, 1 M
0.024
-
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C
0.0379
-
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 35C
0.083
-
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C
0.08
-
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C, 1 M
0.0011
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0019
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.224
-
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
0.457
-
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
-
0.0226
-
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
0.265
-
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
-
80
-
acetyl-tyrosine 4-nitroanilide
-
-
0.0044
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.005
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.04
-
Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.048
-
Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.002
-
Arg-Arg-2-naphthylamide
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0024
-
Arg-Arg-2-naphthylamide
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0006
-
Arg-Arg-Arg
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0011
-
Arg-Arg-Arg
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.25
-
Benzoyl-Arg 2-naphthylamide
-
-
0.5
-
benzoyl-Arg 4-nitroanilide
-
-
0.5
-
benzoyl-Arg 4-nitroanilide
-
benzoyl-Arg ethyl ester
0.48
-
benzoyl-Arg ethyl ester
-
-
0.195
-
benzoyl-Arg-4-nitroanilide
-
pH 7.10, 25C
0.296
-
benzoyl-Arg-4-nitroanilide
-
pH 8.03, 25C
0.301
-
benzoyl-Arg-4-nitroanilide
-
pH 8.00, 25C
1.24
-
benzoyl-Arg-4-nitroanilide
-
pH 8.10, 25C
0.097
-
benzoyl-Arg-beta-naphthylamide
-
pH 8.00, 25C
0.00308
-
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
C9EF60
pH 8.0, 25C
-
0.00776
-
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
C9EF60
pH 8.0, 25C
-
0.00334
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
wild type enzyme, at 37C
0.0044
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
0.00528
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q, at 37C
0.00568
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E610Q, at 37C
0.00904
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609L, at 37C
0.00913
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609F, at 37C
0.0101
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609A, at 37C
0.0113
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q, at 37C
0.0127
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q/E609Q, at 37C
0.0173
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q/E610Q, at 37C
0.0184
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609R, at 37C
0.084
-
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
-
0.54
-
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
0.92
-
Benzoyl-Lys 2-naphthylamide
-
-
0.004
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D638Q
0.0044
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.0045
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D599H
0.0046
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D460T and E494H
0.0047
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D462N
0.005
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D567H
0.0051
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, opdB with polyhistidine affinty tag removed
0.0052
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D460T/D462N
0.0054
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E624H
0.0164
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E578A
0.0334
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E576A
0.003
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.004
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0008
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, wild-type and mutant D567H
0.00088
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, opdB with polyhistidine affinty tag removed
0.001
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E494H, E624H, and D599H
0.0011
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D638Q
0.0021
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D460T
0.0023
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D462N
0.0029
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D460T/D462N
0.0045
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E576A
0.0002
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme; pH 8.0, 37C, recombinant enzyme
0.0034
-
benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.0023
-
benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.0029
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0033
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0012
-
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
-
0.00268
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
wild type enzyme, at 37C
0.00525
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609A, at 37C
0.00586
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E610Q, at 37C
0.00637
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609L, at 37C
0.0069
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609F, at 37C
0.00728
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q/E609Q, at 37C
0.00807
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q, at 37C
0.0127
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q/E610Q, at 37C
0.015
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q, at 37C
0.0215
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609R, at 37C
0.0054
-
benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.002
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D567H
0.0021
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, wild-type and mutant D599A; pH 8.0, 37C, wild-type and mutant D599H
0.0025
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E494H
0.0026
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E624H
0.0028
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D638Q
0.0029
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D460T and D460T/D462N
0.003
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D462N
0.0059
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E578A
0.012
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E576A
0.0023
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.003
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0026
-
benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.0028
-
Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.0019
-
Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.0019
-
Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.0011
-
Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.0026
-
Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.0024
-
Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.001
-
Boc-Leu-Lys-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.0022
-
Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.429
-
DL-Val-Leu-Arg-paranitroanilide
-
-
0.0047
-
Gly-Arg-2-naphthylamide
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0048
-
Gly-Arg-2-naphthylamide
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0016
-
Gly-Gly-Lys-Arg
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.6
-
N-Benzoyl-Arg amide
-
-
0.0038
-
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
-
0.00093
-
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
0.0031
-
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
-
0.31
-
N-Benzyloxycarbonyl-Lys benzyl ester
-
-
0.33
-
N-Benzyloxycarbonyl-Lys benzyl ester
-
-
0.234
-
N-tosyl-Gly-Pro-Arg-paranitroanilide
-
-
0.09
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
pH 8.0, 25C
0.302
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.096
-
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
-
pH 8.0, 25C
0.322
-
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.0513
-
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
-
0.058
-
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
0.0038
-
Phe-Arg-2-naphthylamide
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0049
-
Phe-Arg-2-naphthylamide
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0027
-
Phe-Arg-Arg
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.003
-
Phe-Arg-Arg
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
2.2
-
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.306
-
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
-
1.05
-
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
0.0017
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0021
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0102
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0135
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0013
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0021
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0012
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0019
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0008
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.001
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0012
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0013
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.001
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0011
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.003
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0044
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0122
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0143
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0021
-
tert-butyloxycarbonyl-Val.Gly-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.003
-
tert-butyloxycarbonyl-Val.Gly-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0099
-
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.0081
-
Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
0.23
-
tosyl-Arg methyl ester
-
-
0.47
-
tosyl-Lys-methyl ester
-
N-benzoyl-L-Arg ethyl ester
0.47
-
tosyl-Lys-methyl ester
-
-
0.0002
-
Val-Lys-Arg-Arg
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.0003
-
Val-Lys-Arg-Arg
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.0022
-
Gly-Gly-Lys-Arg
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
additional information
-
additional information
-
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
288
-
2-aminobenzoyl-ADGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
312
-
2-aminobenzoyl-AEGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
312
-
2-aminobenzoyl-AFGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2200
-
2-aminobenzoyl-AFGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
180
-
2-aminobenzoyl-AGDRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
108
-
2-aminobenzoyl-AGERGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
582
-
2-aminobenzoyl-AGFRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1320
-
2-aminobenzoyl-AGFRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
900
-
2-aminobenzoyl-AGGRDAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
780
-
2-aminobenzoyl-AGGREAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
900
-
2-aminobenzoyl-AGGREAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
660
-
2-aminobenzoyl-AGGRFAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1440
-
2-aminobenzoyl-AGGRFAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1020
-
2-aminobenzoyl-AGGRGADQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
402
-
2-aminobenzoyl-AGGRGAEQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
900
-
2-aminobenzoyl-AGGRGAFQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
4080
-
2-aminobenzoyl-AGGRGAFQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
312
-
2-aminobenzoyl-AGGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
354
-
2-aminobenzoyl-AGGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
840
-
2-aminobenzoyl-AGGRGAHQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1500
-
2-aminobenzoyl-AGGRGAHQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
414
-
2-aminobenzoyl-AGGRGAIQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
3300
-
2-aminobenzoyl-AGGRGAIQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1380
-
2-aminobenzoyl-AGGRGALQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2820
-
2-aminobenzoyl-AGGRGALQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
900
-
2-aminobenzoyl-AGGRGAMQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1200
-
2-aminobenzoyl-AGGRGAMQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1020
-
2-aminobenzoyl-AGGRGANQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1860
-
2-aminobenzoyl-AGGRGANQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
108
-
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
186
-
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
312
-
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
390
-
2-aminobenzoyl-AGGRGAQQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
3060
-
2-aminobenzoyl-AGGRGAQQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
564
-
2-aminobenzoyl-AGGRGASQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1620
-
2-aminobenzoyl-AGGRGASQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2220
-
2-aminobenzoyl-AGGRGATQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2880
-
2-aminobenzoyl-AGGRGATQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2160
-
2-aminobenzoyl-AGGRGAVQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2640
-
2-aminobenzoyl-AGGRGAVQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1200
-
2-aminobenzoyl-AGGRGAYQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2760
-
2-aminobenzoyl-AGGRGAYQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
264
-
2-aminobenzoyl-AGGRGDGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
720
-
2-aminobenzoyl-AGGRGDGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
720
-
2-aminobenzoyl-AGGRGEGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1980
-
2-aminobenzoyl-AGGRGEGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
900
-
2-aminobenzoyl-AGGRGFGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2160
-
2-aminobenzoyl-AGGRGFGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
660
-
2-aminobenzoyl-AGGRGGGQG-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
780
-
2-aminobenzoyl-AGGRGGGQG-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
960
-
2-aminobenzoyl-AGGRGHGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1020
-
2-aminobenzoyl-AGGRGHGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1680
-
2-aminobenzoyl-AGGRGIGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1740
-
2-aminobenzoyl-AGGRGIGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1500
-
2-aminobenzoyl-AGGRGLGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
3720
-
2-aminobenzoyl-AGGRGLGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
660
-
2-aminobenzoyl-AGGRGMGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
3240
-
2-aminobenzoyl-AGGRGMGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
432
-
2-aminobenzoyl-AGGRGNGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
660
-
2-aminobenzoyl-AGGRGNGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
336
-
2-aminobenzoyl-AGGRGPGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
450
-
2-aminobenzoyl-AGGRGPGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
552
-
2-aminobenzoyl-AGGRGQGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
840
-
2-aminobenzoyl-AGGRGQGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
588
-
2-aminobenzoyl-AGGRGSGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1380
-
2-aminobenzoyl-AGGRGSGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1020
-
2-aminobenzoyl-AGGRGTGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1140
-
2-aminobenzoyl-AGGRGTGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2400
-
2-aminobenzoyl-AGGRGVGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
3180
-
2-aminobenzoyl-AGGRGVGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1200
-
2-aminobenzoyl-AGGRGYGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1380
-
2-aminobenzoyl-AGGRGYGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
552
-
2-aminobenzoyl-AGGRHAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1620
-
2-aminobenzoyl-AGGRHAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
186
-
2-aminobenzoyl-AGGRIAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
240
-
2-aminobenzoyl-AGGRIAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
246
-
2-aminobenzoyl-AGGRLAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
360
-
2-aminobenzoyl-AGGRLAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1020
-
2-aminobenzoyl-AGGRMAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1140
-
2-aminobenzoyl-AGGRMAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
840
-
2-aminobenzoyl-AGGRNAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
660
-
2-aminobenzoyl-AGGRQAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
720
-
2-aminobenzoyl-AGGRQAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
720
-
2-aminobenzoyl-AGGRSAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1260
-
2-aminobenzoyl-AGGRSAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
660
-
2-aminobenzoyl-AGGRTAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
720
-
2-aminobenzoyl-AGGRTAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
156
-
2-aminobenzoyl-AGGRVAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
288
-
2-aminobenzoyl-AGGRVAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
660
-
2-aminobenzoyl-AGGRYAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
780
-
2-aminobenzoyl-AGGRYAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
720
-
2-aminobenzoyl-AGHRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
396
-
2-aminobenzoyl-AGIRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
780
-
2-aminobenzoyl-AGIRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
264
-
2-aminobenzoyl-AGKRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
444
-
2-aminobenzoyl-AGKRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
900
-
2-aminobenzoyl-AGLRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1140
-
2-aminobenzoyl-AGLRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
780
-
2-aminobenzoyl-AGMRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1440
-
2-aminobenzoyl-AGMRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
426
-
2-aminobenzoyl-AGNRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
468
-
2-aminobenzoyl-AGNRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
216
-
2-aminobenzoyl-AGPRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
720
-
2-aminobenzoyl-AGPRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
600
-
2-aminobenzoyl-AGQRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
960
-
2-aminobenzoyl-AGQRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
3
6
2-aminobenzoyl-AGSRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
102
-
2-aminobenzoyl-AGSRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1140
-
2-aminobenzoyl-AGTRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1620
-
2-aminobenzoyl-AGTRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
84
-
2-aminobenzoyl-AGVRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
294
-
2-aminobenzoyl-AGVRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
720
-
2-aminobenzoyl-AGYRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1440
-
2-aminobenzoyl-AGYRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1140
-
2-aminobenzoyl-AHGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1260
-
2-aminobenzoyl-AHGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1380
-
2-aminobenzoyl-AIGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2100
-
2-aminobenzoyl-AIGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
840
-
2-aminobenzoyl-ALGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1740
-
2-aminobenzoyl-ALGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1380
-
2-aminobenzoyl-AMGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2340
-
2-aminobenzoyl-AMGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
450
-
2-aminobenzoyl-ANGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
132
-
2-aminobenzoyl-APGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
264
-
2-aminobenzoyl-APGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
780
-
2-aminobenzoyl-AQGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2520
-
2-aminobenzoyl-AQGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1020
-
2-aminobenzoyl-ASGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2460
-
2-aminobenzoyl-ASGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
1800
-
2-aminobenzoyl-ATGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
186
-
2-aminobenzoyl-AVGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2700
-
2-aminobenzoyl-AVGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
486
-
2-aminobenzoyl-AYGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
2820
-
2-aminobenzoyl-AYGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
294
-
2-aminobenzoyl-Thr-Arg Cit-Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C
516
-
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 15C, 1 M
1260
-
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C, 3 mM
1390
-
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C
1392
-
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C
1416
-
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 35C
1420
-
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 35C
1218
-
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C
1220
-
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C
12
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
18
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
Q6QDP1, -
pH 8.0, 37C, native enzyme
7.6
-
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
13.9
-
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
-
24.3
-
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
32
-
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
-
1.5
-
Acetyl-Tyr 4-nitroanilide
-
-
23
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
34
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
1
-
Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
2
-
Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
21
-
Arg-Arg-2-naphthylamide
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
30
-
Arg-Arg-2-naphthylamide
Q6QDP1, -
pH 8.0, 37C, native enzyme
29
-
Arg-Arg-Arg
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
38
-
Arg-Arg-Arg
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.024
-
Benzoyl-Arg 2-naphthylamide
-
-
5496
-
benzoyl-Arg-4-nitroanilide
-
pH 7.10, 25C
5500
-
benzoyl-Arg-4-nitroanilide
-
pH 7.10, 25C
6850
-
benzoyl-Arg-4-nitroanilide
-
pH 8.00, 25C
6852
-
benzoyl-Arg-4-nitroanilide
-
pH 8.00, 25C
11290
-
benzoyl-Arg-4-nitroanilide
-
pH 8.03, 25C
11300
-
benzoyl-Arg-4-nitroanilide
-
pH 8.03, 25C
4848
-
benzoyl-Arg-beta-naphthylamide
-
pH 8.00, 25C
4850
-
benzoyl-Arg-beta-naphthylamide
-
pH 8.00, 25C
6830
-
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
C9EF60
pH 8.0, 25C
-
4250
-
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
C9EF60
pH 8.0, 25C
-
0.016
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
0.758
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q, at 37C
1.42
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609R, at 37C
1.46
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609F, at 37C
1.64
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q/E609Q, at 37C
4.98
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609L, at 37C
6.84
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609A, at 37C
7.81
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q, at 37C
8.29
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
wild type enzyme, at 37C
9.36
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E610Q, at 37C
11.2
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q/E610Q, at 37C
21.2
-
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
-
201.7
-
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
4.4
-
Benzoyl-Lys 2-naphthylamide
-
-
120
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E576A
240
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E578A
420
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D460T/D462N
540
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, wild-type, mutant E494H, D599H and D567H
600
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, opdB with polyhistidine affinity tag removed, mutant D460T, D462N and E624H
660
-
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D638Q
17
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
26
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
540
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E576A and E578A
1080
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D460T/D462N
1320
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D460T
1380
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D462N
1740
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C wild-type and mutant D567H
1800
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D599H
1860
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D638Q
1980
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E494H and E624H
2040
-
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, opdB with polyhistidine affinty tag removed
59
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
64
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
900
-
benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
1500
-
benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
33
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C,recombinant enzyme
48
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.013
-
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
-
1.24
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q, at 37C
1.58
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q/E609Q, at 37C
1.77
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609F, at 37C
5.03
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609L, at 37C
5.37
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609R, at 37C
5.42
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609A, at 37C
9.53
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q/E610Q, at 37C
13.3
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
wild type enzyme, at 37C
15.2
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E610Q, at 37C
15.8
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q, at 37C
420
-
benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
420
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E576A
540
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant E578A
1020
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D460T/D462N
1080
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D460T and E494H
1140
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D462N
1200
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D599A and D638Q
1260
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
1320
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C, mutant D567H and E624H
59
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
77
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
900
-
benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
1020
-
Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
1380
-
Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
1200
-
Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
1560
-
Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
1080
-
Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
960
-
Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
1080
-
Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
11
-
Gly-Arg-2-naphthylamide
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
12
-
Gly-Arg-2-naphthylamide
Q6QDP1, -
pH 8.0, 37C, native enzyme
40
-
Gly-Gly-Lys-Arg
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
54
-
Gly-Gly-Lys-Arg
Q6QDP1, -
pH 8.0, 37C, native enzyme
197
-
N-benzoyl-Arg ethyl ester
-
-
3.5
-
N-benzoyl-arginine amide
-
-
57
-
N-benzoyl-arginine amide
-
-
0.02
-
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
-
0.01
-
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
0.011
-
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
-
43
-
N-benzyloxycarbonyl-Lys 4-nitrophenyl ester
-
-
55
-
N-Benzyloxycarbonyl-Lys benzyl ester
-
-
14.5
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
pH 8.0, 25C
37.7
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
53.8
-
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
-
presence of 50 mM Ca2+, pH 8.0, 25C
55.5
-
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
-
pH 8.0, 25C
2
8
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
35.2
-
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
-
17
-
Phe-Arg-2-naphthylamide
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
18
-
Phe-Arg-2-naphthylamide
Q6QDP1, -
pH 8.0, 37C, native enzyme
16
-
Phe-Arg-Arg
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
21
-
Phe-Arg-Arg
Q6QDP1, -
pH 8.0, 37C, native enzyme
1
-
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.5
-
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
1.2
-
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
-
102
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
122
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, ecombinant mutant enzyme S563A
9
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
12
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
81
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
101
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
55
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C,recombinant enzyme
76
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
108
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
154
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
57
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
74
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
94
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
132
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
18
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
22
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
8
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
12
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
58
-
tert-butyloxycarbonyl-Val.Gly-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
64
-
tert-butyloxycarbonyl-Val.Gly-Arg-7-amido-4-methylcoumarin
Q6QDP1, -
pH 8.0, 37C, native enzyme
780
-
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
1080
-
Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide
Q9L6C8, -
pH 8.0, 37C
60
-
tosyl-Arg methyl ester
-
-
55
-
Tosyl-Lys methyl ester
-
-
38
-
Val-Lys-Arg-Arg
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
44
-
Val-Lys-Arg-Arg
Q6QDP1, -
pH 8.0, 37C, native enzyme
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
30
-
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0
34
-
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0
10.8
-
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0
120
-
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0
2218000
-
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
C9EF60
pH 8.0, 25C
0
547700
-
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
C9EF60
pH 8.0, 25C
0
3.6
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
227853
50
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609F, at 37C
227853
67
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q, at 37C
227853
77
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609R, at 37C
227853
129
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q/E609Q, at 37C
227853
551
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609L, at 37C
227853
647
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q/E610Q, at 37C
227853
677
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609A, at 37C
227853
1480
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q, at 37C
227853
1650
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E610Q, at 37C
227853
2480
-
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
wild type enzyme, at 37C
227853
251
-
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0
377
-
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0
1
-
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
0
83
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q, at 37C
254077
217
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q/E609Q, at 37C
254077
250
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609R, at 37C
254077
265
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609F, at 37C
254077
750
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q/E610Q, at 37C
254077
790
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609L, at 37C
254077
1030
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609A, at 37C
254077
1960
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q, at 37C
254077
2590
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E610Q, at 37C
254077
4960
-
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
wild type enzyme, at 37C
254077
5.3
-
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
0
1.1
-
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
254651
3.6
-
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
-, Q4QHU7
21C, pH not specified in the publication
0
125
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
208155
161
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
pH 8.0, 25C
208155
2
-
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
-
presence of 50 mM Ca2+, pH 8.0, 25C
320365
5.8
-
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
-
pH 8.0, 25C
320365
485
-
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0
680
-
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0
0.07
-
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide
-
pH 8.0, 25C
235938
0.4
-
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
235938
0.5
-
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0
3
-
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.4
-
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
0.188
-
agmatine
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.207
-
agmatine
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.00000181
-
antipain
Q382P7
-
0.0000081
-
antipain
Q9L6C8, -
pH 8.0, 37C
0.001
-
antipain
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.002
-
antipain
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.1867
-
Benzamidine
Q9L6C8, -
pH 8.0, 37C
0.218
-
Benzamidine
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.254
-
Benzamidine
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.05
-
benzoyl-Arg-4-nitroanilide
-
pH 8.10, 25C
0.027
-
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
0.19
-
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
-
0.00003
-
Leupeptin
Q382P7
-
0.0004
-
Leupeptin
Q9L6C8, -
pH 8.0, 37C
0.021
-
Leupeptin
Q6QDP1, -
pH 8.0, 37C, native enzyme
0.029
-
Leupeptin
Q6QDP1, -
pH 8.0, 37C, recombinant enzyme
0.045
-
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25C
-
0.126
-
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
-
0.1171
-
p-aminobenzamidine
Q9L6C8, -
pH 8.0, 37C
0.0034
-
Pentamidine
Q382P7
-
0.00001
-
protamine
Q382P7
-
0.0067
-
suramin
Q382P7
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.01
-
trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester
-
IC50: about 0.01 mM. Oligopeptidase B is much more sensitive than oligopeptidase B
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0001
-
-
mutant enzyme E607Q/E609Q, towards benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin, at 37C
0.000323
-
-
mutant enzyme E607Q, towards benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin, at 37C
0.011
-
-
mutant enzyme E609Q/E610Q, towards benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin, at 37C
0.017
-
Q6QDP1, -
-
0.0209
-
-
mutant enzyme E610Q, towards benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin, at 37C
0.0221
-
-
mutant enzyme E609Q, towards benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin, at 37C
0.0279
-
-
wild type enzyme, towards benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin, at 37C
176
-
-
purified enzyme
additional information
-
-
-
additional information
-
P24555
the specific activity of OpdB in Escherichia coli increases under conditions where the carbon levels are limiting
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.3
-
-
pH optimum in the presence of NaCl
7.5
9
-
N-benzoyl-DL-arginine 4-nitroanilide
7.6
8.9
-
azocasein
8.5
-
Q6QDP1, -
hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
8.8
-
-
pH optimum in the absence of NaCl
additional information
-
-
pI: 5.2
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
10.5
Q6QDP1, -
pH 7.0: about 50% of maximal activity, pH 10.5: about 50% of maximal activity, hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
additional information
-
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
optimum temperature for the reactions of amide substrates slightly below
25
-
-
optimum temperature for the reactions of amide substrates slightly below
25
-
-
the abnormally low temperature optimum may be caused by the disruption of the hydrogen bond formed between the enzyme and the NH group of the scissile peptide bond
37
-
-
assay at
39
-
-
benzoyl-arginine ethyl ester
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
-
-
activity is reduced by 90% at 60C for 10 min
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.5
-
-
estimated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
enzyme is secreted
Manually annotated by BRENDA team
-
harvested at late log-phase
Manually annotated by BRENDA team
additional information
Q6QDP1, -
Trypanosoma evansi releases oligopeptidase B into the plasma of infected hosts
Manually annotated by BRENDA team
additional information
A7XAB0, -
oligopeptidase B gene is expressed in all cycle stages
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
58000
-
-
E. coli, sucrose density gradient centrifugation, gel filtration
78000
-
B3VI58, -
His-tagged enzyme, SDS-PAGE
78970
-
Q9L6C8, -
predicted from opdB gene open reading frame
81000
-
-
E. coli, gel filtration
81860
-
-
E. coli, calculation from nucleotide sequence
81860
-
-
amino acid sequence
83490
-
A7XAB0, -
predicted from cDNA
120000
-
Q4CW30
-
160000
-
-
gel filtration
additional information
-
-
Ser532 is the reactive serine residue
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 60000, SDS-PAGE
?
-
x * 60000, SDS-PAGE
?
-
x * 80000, SDS-PAGE under reducing and non-reducing conditions
?
-
x * 78000, SDS-PAGE
homodimer
-
2 * 84000, calculated from amino acid sequence
monomer
-
1 * 80000, SDS-PAGE without mercaptoethanol
monomer
-
1 * 58000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
using the hanging-drop vapour-diffusion technique in 7%(w/v) polyethylene glycol 6000, 1 M LiCl, 0.1 M bis-tris propane pH 7.5. Diffraction data to 2.7 A resolution are collected using synchrotron radiation. The crystals belong to space group P3121 or P3221, with unit-cell parameters a = b = 124.5, c = 249.9 A. A complete data set to 2.7 A is collected using synchrotron radiation
A7XAB0, -
in complex with the inhibitor antipain and as mercury derivative, to 1.65 and 2.0 A resolution, respectively. OPB comprises two domains, a catalytic and a propeller domain, linked together by a hinge region. Residue E621 plays a critical role in the S1 binding pocket and, along with F603, is largely responsible for the enzyme substrate specificity in P1. In the S2 binding pocket, Y499 is important for substrate stability. Residue E623 forms an inter-domain hydrogen bond
-, Q4QHU7
crystal structure solved at 2.7 A resolution
Q382P7
using the hanging-drop vapour-diffusion technique in 7% (w/v) polyethylene glycol 6000, 1 M LiCl, 0.1 M bis-tris propane pH 7.5. Diffraction data to 2.7 A resolution are collected using synchrotron radiation. The crystals belong to space group P3121 or P3221, with unit-cell parameters a = b = 124.5, c = 249.9 A. A complete data set to 2.7 A is collected using synchrotron radiation
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
9.5
-
stable
6
-
-
quick inactivation below
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
rapid loss of activity above
40
-
-
3 h, stable
45
53
-
following treatment at 45C for 20 min the relative activity of wild type enzyme is 89%, the melting temperature of the wild type enzyme is at 53.3C
45
-
-
30 min, pH 8.0, 50% loss of activity
45
-
-
15 min, stable below
50
-
-
15 min, complete inactivation
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ca2+ decreases stability
-
Repeated freezing and thawing does not affect esterase activity
-
the wild type enzyme is not digested by trypsin at 25C
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimethyl sulfoxide
-
assay in presence of 2% dimethyl sulfoxide
urea
-
the wild type enzyme is resistant to treatment with 3 M urea
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, 5 weeks, without or with 25 and 50 mM CaCl2, the remaining activities are 88%, 50% and 32% respectively
-
-80C, enzyme form A loses its activity after 3 months of storage, enzyme form B remains stable for more than one year
-
-80C, maximal stability in dilute solutions at protein concentrations no less than 0.3 mg/ml, pH 7.8
-
-20C, stable for several days
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
by affinity chromatography and separation of enzyme species A and B
-
HB101 gene cloned and expressed in JM83
-
recombinant
-
purified by using using metal-affinity chromatography
A7XAB0, -
QSepharose column chromatography, ultrafiltration, Ni-NTA-agarose column chromatography, and immobilized basic pancreatic trypsin inhibitor-Sepharose column chromatography
B3VI58, -
TALON metal affinity resin column chromatography
-
Ni-NTA agarose column chromatography
-
purified by using metal-affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Escherichia coli HB101 gene cloned and expressed in Escherichia coli JM83
-
fusion expression of oligopeptidase B with an N-terminal histidine tag using pET28a as the expression vector. Although most of the recombinant OpdB is produced as inclusion bodies, the solubility of the recombinant protease increases significantly when expression temperature shifts from 37C to 30C
-
location of the protease II gene on the physical map of the Escherichia coli chromosome
-
overexpressed in Escherichia coli
-
overexpressed in Escherichia coli JM83
-
Oligopeptidase B is overexpressed in Escherichia coli as an N-terminally hexahistidine-tagged fusion protein
A7XAB0, -
expression in Escherichia coli
-, Q4QHU7
opdB gene PCR amplified and expressed in Escherichia coli BL21(lambdaDE3)
Q9L6C8, -
expressed in Escherichia coli BL-21(DE3) cells
B3VI58, -
expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
-
Oligopeptidase B is overexpressed in Escherichia coli as an N-terminally hexahistidine-tagged fusion protein
-
expressed in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Y452F
-
site-specific mutagenesis
D460T
Q9L6C8, -
site-mutated variant
D460T/D462N
Q9L6C8, -
site-mutated variant
D462N
Q9L6C8, -
site-mutated variant
D567H
Q9L6C8, -
site-mutated variant
D599H
Q9L6C8, -
site-mutated variant
D638Q
Q9L6C8, -
site-mutated variant
E494H
Q9L6C8, -
site-mutated variant
E576A
Q9L6C8, -
site-mutated variant
E576A/E578A
Q9L6C8, -
site-mutated variant
E576R/E578R
Q9L6C8, -
site-mutated variant
E576W/E578T
Q9L6C8, -
site-mutated variant
E578A
Q9L6C8, -
site-mutated variant
E624H
Q9L6C8, -
site-mutated variant
C544A
-
the enzymatic activity of the Cys-free mutant is markedly activated by the general thiol-reacting reagent dithiothreitol, oxidized glutathione, and reduced glutathione,the mutant is insensitive to the treatment of the SH-blocking reagent N-ethylmaleimide or H2O2
C256S
-
750fold reduction in inhibition rate constant for iodoacetic acid and more than 100fold reduction in inhibition rate constant for NEM compared to wild-type enzyme
C559S
Q382P7
mutant shows significant decrease in activation by reducing agents. This residue may thus mediate the activity enhancing effect of reducing agents
C597S
Q382P7
mutant shows significant decrease in activation by reducing agents. This residue may thus mediate the activity enhancing effect of reducing agents
E607Q
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E607Q/E609Q
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E609A
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E609F
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E609L
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E609Q
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E609Q/E610Q
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E609R
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
S563A
Q6QDP1, -
catalytically inactive OpdB variant, no activity with benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin and tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
additional information
P24555
mutation of Tyr452 confirmed that this residue is involved in the catalytic mechanism by stabilising the intermediate consisting of an oxoanion. Furthermore, it is shown that this oxanion binding site prevents non-productive substrate binding
E610Q
-
the mutant shows reduced kcat/Km compared to the wild type enzyme, the mutation results in a marked decrease in thermal stability, the mutant is inactivated to 63% of the control by 3 M urea
additional information
Q382P7
cys256 is identified as the reactive cysteine residue responsible for inactivation by N-ethylmaleimide and iodoacetic acid
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
involved in host cell invasion, important target for drug design
medicine
Q9L6C8, -
potential theraputic target in infectious disease