Information on EC 3.4.21.83 - Oligopeptidase B

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.4.21.83
-
RECOMMENDED NAME
GeneOntology No.
Oligopeptidase B
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of -Arg-/-, -Lys-/- bonds in oligopeptides, even when P1' residue is proline
show the reaction diagram
hydrolysis of Arg-+-, Lys-+- bonds in oligopeptides, even when P1' residue is proline, the term-+- depicts the point of cleavage
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-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY hide
57657-67-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
strain 94
UniProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-ADGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ADGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AEGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AEGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AFGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AFGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGDRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGDR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGERGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGER + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGFRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGFR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRDAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + DAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGREAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + EAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRFAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + FAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGADQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GADQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRGAEQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAEQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAFQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAFQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGAHQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAHQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAIQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAIQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGALQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GALQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGAMQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAMQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGANQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GANQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAPQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
2-aminobenzoyl-AGGRGAQQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAQQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGASQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GASQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRGATQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GATQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAVQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAVQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGAYQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAYQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGDGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GDGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGEGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GEGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGFGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GFGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGGGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GGGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGHGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GHGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGIGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GIGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGLGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GLGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGMGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GMGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGNGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GNGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGPGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GPGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGQGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GQGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGSGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGRG + Ser-Gly-Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGTGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GTGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
show the reaction diagram
2-aminobenzoyl-AGGRGVGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GVGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRGYGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GYGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRHAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + HAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRIAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + IAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRLAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + LAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRMAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + MAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRNAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + NAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRPAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + PAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRQAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + QAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRSAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + SAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRTAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + TAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRVAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + VAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGGRYAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + YAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGHRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGHR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGIRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGIR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGKRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGKR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGLRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGLR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGMRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGMR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGNRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGNR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGPRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGPR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGQRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGQR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGRRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGRR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGRRRAQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
2-aminobenzoyl-AGSRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGSR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGTRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGTR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGVRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGVR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AGYRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGYR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AHGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AHGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AHKRYSHQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
2-aminobenzoyl-AIGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AIGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-ALGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ALGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AMGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AMGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AMRRTISQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
2-aminobenzoyl-ANGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ANGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-APGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AQGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AQGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-ASGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ASGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-ATGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ATGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AVGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AVGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-AYGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AYGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-FRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
2-aminobenzoyl-FRSSR-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-Phe-Arg + Ser-Ser-Arg-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-GFSPFRSSRIGIKEEQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-GFSPFR + SSRIGIKEEQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-GFSPFRSSRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
2-aminobenzoyl-LGMISLMKRPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-LGMISLMK + Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-MISLMKRPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-MISLMK + Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-RPPGFSPFRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-RPPGFSPFR + Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-RPPGFSPFRSSRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-RPPGFSPFR + Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
2-aminobenzoyl-Thr-Arg Cit-Phe(NO2)-Ser-Leu-NH2 + H2O
?
show the reaction diagram
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-
-
?
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 + H2O
?
show the reaction diagram
-
-
-
?
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2 + H2O
?
show the reaction diagram
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-
-
?
2-aminobenzoyl-Thr-Cit-Cit-Phe(NO2)-Ser-Leu-NH2 + H2O
?
show the reaction diagram
-
-
-
?
3-(2-furyl)acryloyl-Tyr-Lys-Arg + H2O
3-(2-furyl)acryloyl-Tyr-Lys + Arg
show the reaction diagram
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-
-
?
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide + H2O
acetyl-L-Leu-L-Leu-L-Arg + 4-nitroaniline
show the reaction diagram
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?
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide + H2O
acetyl-L-Leu-L-Lys-L-Arg + 4-nitroaniline
show the reaction diagram
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?
acetyl-Leu-Leu-Arg-4-nitroanilide + H2O
acetyl-Leu-Leu-Arg + 4-nitroaniline
show the reaction diagram
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?
Acetyl-Tyr 4-nitroanilide + H2O
Acetyl-Tyr + 4-nitroaniline
show the reaction diagram
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-
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adrenocorticotropic hormone + H2O
SYSMEHFR + WGK + WGKPVGK + VYP
show the reaction diagram
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-
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?
Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
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?
Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
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?
Arg-Arg-2-naphthylamide + H2O
?
show the reaction diagram
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?
Arg-Arg-Arg-OH + H2O
Arg-Arg + Arg
show the reaction diagram
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?
Atrial natriuretic factor + H2O
?
show the reaction diagram
azocasein + H2O
?
show the reaction diagram
Benzoyl-Arg 2-naphthylamide + H2O
Benzoyl-Arg + 2-naphthylamine
show the reaction diagram
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benzoyl-Arg-2-naphthylamide + H2O
?
show the reaction diagram
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-
?
benzoyl-Arg-4-nitroanilide + H2O
?
show the reaction diagram
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-
?
benzoyl-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
benzoyl-Arg-beta-naphthylamide + H2O
?
show the reaction diagram
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-
?
benzoyl-Arg-Et + H2O
?
show the reaction diagram
benzoyl-Arg-NH2 + H2O
?
show the reaction diagram
benzoyl-arginine ethyl ester + H2O
?
show the reaction diagram
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?
benzoyl-citrulline-Et + H2O
?
show the reaction diagram
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Gly-Gly-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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-
?
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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-
-
?
benzoyl-L-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O
benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline
show the reaction diagram
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-
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?
Benzoyl-Lys 2-naphthylamide + H2O
Benzoyl-Lys + 2-naphthylamine
show the reaction diagram
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benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
benzoyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl 7-amide+ H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Arg-Arg-7-(4-methylcoumaryl)amide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Gly-L-Arg-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-Leu-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Leu-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Val-L-Val-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide + H2O
Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
Boc-Leu-Lys-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
CBZ-L-Arg-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
weak substrate
-
-
?
Denatured casein + H2O
?
show the reaction diagram
-
weak
-
-
-
DL-Val-Leu-Arg-paranitroanilide + H2O
DL-Val-Leu-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Glucagon + H2O
?
show the reaction diagram
-
wild type enzyme cleaves Lys12-Tyr13, Arg17-Arg18, and Arg18-Ala19 bonds of glucagon
-
-
?
Gly-Arg-2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
?
Gly-Gly-Lys-Arg + H2O
Gly-Gly-Lys-Arg + Arg
show the reaction diagram
-
-
-
?
histone + H2O
?
show the reaction diagram
human histones H1, H2A, H3 and H4, restricted proteolysis
-
?
Insulin B-chain + H2O
?
show the reaction diagram
L-pGlu-Gly-L-Arg-7-amido-4-methylcoumarin + H2O
L-pGlu-Gly-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low hydrolytic rate
-
-
?
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin + H2O
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low hydrolytic rate
-
-
?
L-Phe-L-Arg 2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
-
L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
L-Pro-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-Benzoyl-DL-Arg 4-nitroanilide + H2O
N-Benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
N-Benzoyl-L-Arg ethyl ester + H2O
N-Benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
-
-
-
N-benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
N-Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O
N-Benzyloxycarbonyl-Gly + 4-nitrophenol
show the reaction diagram
-
weak
-
-
-
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Gly-Gly-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
N-Benzyloxycarbonyl-Lys benzyl ester + H2O
N-Benzyloxycarbonyl-Lys + benzol
show the reaction diagram
-
-
-
-
-
N-Carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O
N-Carbobenzoxy-L-Lys + 4-nitrophenol
show the reaction diagram
-
-
-
-
-
N-tosyl-Gly-Pro-Arg-paranitroanilide + H2O
N-tosyl-Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
68% of the activity with carbobenzoxy-Arg-Arg-4-methylcoumaryl-7-amide
-
-
?
Nalpha-benzoyl-DL-Arg-4-nitroanilide + H2O
Nalpha-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
Nalpha-benzyloxycarbonyl-L-Ala-L-Ala-L-Arg-4-nitroanilide + H2O
Nalpha-benzyloxycarbonyl-L-Ala-L-Ala-L-Arg + 4-nitroaniline
show the reaction diagram
-
poor substrate
-
-
?
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester + H2O
Nalpha-benzyloxycarbonyl-L-lysine + phenylmethanethiol
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide + H2O
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Phe-Arg-2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
?
Phe-Arg-Arg + H2O
Phe-Arg + Arg
show the reaction diagram
-
-
-
?
RRRPRPPRLPRPRPR + 2 H2O
RR + RPRPPR + LPRPRPR
show the reaction diagram
-
proline-rich peptide Bac(1-16)
-
-
?
RRRPRPPYLPRPRPPPFF + 2 H2O
RR + RPRPPYLPRPRPPPFF
show the reaction diagram
-
proline-rich peptide PR-39(1-18)
-
-
?
succinyl-Gly-L-Pro-L-Lys-4-nitroanilide + H2O
succinyl-Gly-L-Pro-L-Lys + 4-nitroaniline
show the reaction diagram
-
poor substrate
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
best substrate, the hydrolytic activity is observed only in the presence of NaCl and the thiol-reacting reagents dithiothreitol, reduced glutathione, and oxidized glutathione at pH 8.5 (50 mM Tris-HCl)
-
-
?
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
specific substrate for Escherichia coli oligopeptidase B, 20% of the activity with carbobenzoxy-Arg-Arg-4-methylcoumaryl-7-amide
-
-
?
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
?
Tosyl-Arg methyl ester + H2O
Tosyl-Arg + methanol
show the reaction diagram
-
-
-
-
-
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
tosyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
tosyl-Gly-L-Pro-L-Lys-7-amido-4-methylcoumarin + H2O
tosyl-Gly-L-Pro-L-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Tosyl-Lys methyl ester + H2O
Tosyl-Lys + benzyl alcohol
show the reaction diagram
-
-
-
-
-
Val-Lys-Arg-Arg + H2O
Val-Lys-Arg + Arg
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Atrial natriuretic factor + H2O
?
show the reaction diagram
Q6QDP1
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3,4-dichloroisocoumarin
4-(2-aminoethyl)benzenesulfonyl fluoride
4-(2-aminoethyl)benzenesulfonylfluoride
non-peptide irreversible serine-peptidase inhibitor
4-methylumbelliferyl-p-guanidobenzoate
active site titrant
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
-
acetyl-Thr-Arg-Arg
-
-
agmatine
reversible
antipain
antipain dihydrochloride
-
1 mM, 79% remaining activity
Aromatic amidines
-
-
-
benzamidine
benzoyl-Arg-4-nitroanilide
-
enzyme is inhibited by high concentrations of substrate
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
-
bovine basic pancreatic trypsin inhibitor
-
substrate Nalpha-benzoyl-DL-Arg-4-nitroanilide, competitive
-
Ca2+
0.01 mM, 69% residual activity
chymostatin
-
0,1 mM, 45% inhibition
diisopropylfluorophosphate
non-peptide irreversible serine-peptidase inhibitor
EDTA
-
1.3 mM, 98% remaining activity, 20 mM, 57% remaining activity
iodoacetamide
-
-
iodoacetic acid
-
L-arginine
-
-
Leupeptin
m-phenanthroline
-
substrates Nalpha-benzoyl-DL-Arg-4-nitroanilide, acetyl-Leu-Leu-Arg-4-nitroanilide, anticompetitive
methanol
incubation in 20% (v/v) methanol (10-20 h, 4C) results in partial decomposition of the high molecular weight PSP-GroEL complex with precipitation of denatured chaperonin and a small (no more than 10%) loss of PSP activity
Mg2+
0.01 mM, 73% residual activity
Mn2+
0.01 mM, 73% residual activity
N-ethylmaleimide
NaCl
-
loss of almost 50 and 70% of its activity in the presence of 0.2 and 1.0 M NaCl, respectively. Presence of NaCl does not disrupt the dimeric structure
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
-
NEM
-
Cys256 is the reactive cysteine residue that mediates OpdB inhibition. Cys256 adducts occlude the P1 substrate-binding site, preventing substrate binding
o-phenanthroline
-
substrates Nalpha-benzoyl-DL-Arg-4-nitroanilide, acetyl-Leu-Leu-Arg-4-nitroanilide, anticompetitive
p-aminobenzamidine
p-chloromercuribenzoate
non-peptide irreversible serine-peptidase inhibitor
Pefabloc SC
Pentamidine
reversible competitive inhibitor
Pepstatin
-
1 microM, 94% remaining activity
peptidyl chloromethyl ketone
irreversible peptidyl inhibitors
peptidyl diazomethyl ketone
irreversible peptidyl inhibitors
peptidyl phosphonate alpha-aminoalkyl diphenyl ester
irreversible peptidyl inhibitors
Phe-Pro-Arg-chloromethylketone
0.01 mM, no residual activity
phenylmethane sulfonyl fluoride
irreversible inhibition
phenylmethanesulfonyl fluoride
irreversible
phosphoramidon
-
0.57 mM, 96% remaining activity
protamine
reversible competitive inhibitor
protamines
-
protamines, basic 30-32 residue peptides that are rich in Arg residues, are potent inhibitors of OpdB
-
suramin
partial non-competitive inhibitor
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin
-
in the case of added dithiothreitol, reduced glutathione, or oxidized glutathione, a high concentration of the substrate tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin inhibits the enzyme activity. By contrast, addition of 1 M NaCl to either dithiothreitol, reduced glutathione, or oxidized glutathione or 1 M NaCl alone prevents substrate inhibition
Tos-LysCH2Cl
non-peptide irreversible serine-peptidase inhibitor
tosyl-L-Lys-chloromethylketone
0.1 mM, no residual activity
tosyl-Leu chloromethyl ketone
-
-
trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester
-
IC50: about 0.01 mM. Oligopeptidase B is much more sensitive than oligopeptidase B
ZnCl2
-
substrate Nalpha-benzoyl-DL-Arg-4-nitroanilide, noncompetitive
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Aprotinin
-
0.3 microM, 103% activity
bestatin
-
0.13 mM, 105% activity
dithiothreitol
E-64
-
5 microM, 114% activity
Leupeptin
-
1 microM, 116% activity
NaCl
-
enzymatic activity is markedly enhanced by high NaCl concentration, NaCl affects the P1 preference of OpdB synergistically in a substrate-dependent manner
oxidized glutathione
-
oxidized glutathione enhances OpdB activity and affects the P1 preference of OpdB synergistically in a substrate-dependent manner
reduced glutathione
-
enzymatic activity is markedly enhanced by reduced glutathione at submillimolar level which affects the P1 preference of OpdB synergistically in a substrate-dependent manner
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0036
2-aminobenzoyl-ADGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.039
2-aminobenzoyl-AEGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0032 - 0.0034
2-aminobenzoyl-AFGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.013
2-aminobenzoyl-AGDRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0033
2-aminobenzoyl-AGERGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0024 - 0.0031
2-aminobenzoyl-AGFRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0015 - 0.0045
2-aminobenzoyl-AGGRDAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0014 - 0.0065
2-aminobenzoyl-AGGREAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0003 - 0.0027
2-aminobenzoyl-AGGRFAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0057
2-aminobenzoyl-AGGRGADQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.021
2-aminobenzoyl-AGGRGAEQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0062 - 0.0097
2-aminobenzoyl-AGGRGAFQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0011 - 0.0072
2-aminobenzoyl-AGGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0034 - 0.0037
2-aminobenzoyl-AGGRGAHQ-N-(2,4-dinitrophenyl)ethylenediamine
0.011 - 0.031
2-aminobenzoyl-AGGRGAIQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0073 - 0.012
2-aminobenzoyl-AGGRGALQ-N-(2,4-dinitrophenyl)ethylenediamine
0.003 - 0.0031
2-aminobenzoyl-AGGRGAMQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0059 - 0.018
2-aminobenzoyl-AGGRGANQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0011 - 0.013
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0048 - 0.0088
2-aminobenzoyl-AGGRGAQQ-N-(2,4-dinitrophenyl)ethylenediamine
0.002 - 0.012
2-aminobenzoyl-AGGRGASQ-N-(2,4-dinitrophenyl)ethylenediamine
0.006 - 0.027
2-aminobenzoyl-AGGRGATQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0091 - 0.021
2-aminobenzoyl-AGGRGAVQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0057 - 0.011
2-aminobenzoyl-AGGRGAYQ-N-(2,4-dinitrophenyl)ethylenediamine
0.008 - 0.012
2-aminobenzoyl-AGGRGDGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.01 - 0.138
2-aminobenzoyl-AGGRGEGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0021 - 0.012
2-aminobenzoyl-AGGRGFGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0062 - 0.014
2-aminobenzoyl-AGGRGGGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0028 - 0.0033
2-aminobenzoyl-AGGRGHGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0032 - 0.011
2-aminobenzoyl-AGGRGIGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0052 - 0.024
2-aminobenzoyl-AGGRGLGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.003 - 0.02
2-aminobenzoyl-AGGRGMGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0046 - 0.0092
2-aminobenzoyl-AGGRGNGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0012 - 0.0098
2-aminobenzoyl-AGGRGPGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0023 - 0.01
2-aminobenzoyl-AGGRGQGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0032 - 0.0037
2-aminobenzoyl-AGGRGSGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0058 - 0.013
2-aminobenzoyl-AGGRGTGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.02 - 0.031
2-aminobenzoyl-AGGRGVGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0039 - 0.0044
2-aminobenzoyl-AGGRGYGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0005 - 0.0017
2-aminobenzoyl-AGGRHAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0003 - 0.0005
2-aminobenzoyl-AGGRIAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0006 - 0.0013
2-aminobenzoyl-AGGRLAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0069 - 0.0083
2-aminobenzoyl-AGGRMAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0004 - 0.0013
2-aminobenzoyl-AGGRNAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0005 - 0.0007
2-aminobenzoyl-AGGRQAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0012
2-aminobenzoyl-AGGRSAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0003 - 0.0009
2-aminobenzoyl-AGGRTAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0001 - 0.0014
2-aminobenzoyl-AGGRVAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0004 - 0.0006
2-aminobenzoyl-AGGRYAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0063
2-aminobenzoyl-AGHRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0012 - 0.01
2-aminobenzoyl-AGIRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0018 - 0.0048
2-aminobenzoyl-AGKRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0019 - 0.0077
2-aminobenzoyl-AGLRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0025 - 0.0029
2-aminobenzoyl-AGMRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.004 - 0.023
2-aminobenzoyl-AGNRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0082 - 0.016
2-aminobenzoyl-AGPRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0014 - 0.0059
2-aminobenzoyl-AGQRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0004 - 0.0006
2-aminobenzoyl-AGSRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0019 - 0.0075
2-aminobenzoyl-AGTRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0014 - 0.0039
2-aminobenzoyl-AGVRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0077 - 0.014
2-aminobenzoyl-AGYRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0032 - 0.0035
2-aminobenzoyl-AHGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.002 - 0.0044
2-aminobenzoyl-AIGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0013 - 0.0026
2-aminobenzoyl-ALGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0009 - 0.0059
2-aminobenzoyl-AMGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0083
2-aminobenzoyl-ANGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.001 - 0.0019
2-aminobenzoyl-APGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0019 - 0.0024
2-aminobenzoyl-AQGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0023 - 0.0074
2-aminobenzoyl-ASGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0018 - 0.0082
2-aminobenzoyl-ATGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0026 - 0.013
2-aminobenzoyl-AVGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0018 - 0.003
2-aminobenzoyl-AYGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0298
2-aminobenzoyl-Thr-Arg Cit-Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C
0.0048 - 0.083
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
0.08
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C, 1 M
0.0011 - 0.0019
3-(2-furyl)acryloyl-Tyr-Lys-Arg
0.224 - 0.457
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
0.0226 - 0.265
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
80
acetyl-tyrosine 4-nitroanilide
-
-
0.0044 - 0.005
Ala-Phe-Lys-7-amido-4-methylcoumarin
0.04 - 0.048
Arg-7-amido-4-methylcoumarin
0.002 - 0.0024
Arg-Arg-2-naphthylamide
0.0006 - 0.0011
Arg-Arg-Arg
0.25
Benzoyl-Arg 2-naphthylamide
-
-
0.5
benzoyl-Arg 4-nitroanilide
0.48
benzoyl-Arg ethyl ester
-
-
0.195 - 1.24
benzoyl-Arg-4-nitroanilide
0.00268 - 0.0411
benzoyl-Arg-7-amido-4-methylcoumarin
0.097
benzoyl-Arg-beta-naphthylamide
-
pH 8.00, 25C
0.00308
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
pH 8.0, 25C
0.00776
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
pH 8.0, 25C
0.00334 - 0.0184
benzoyl-L-Arg-7-amido-4-methylcoumarin
0.084 - 0.54
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
0.92
Benzoyl-Lys 2-naphthylamide
-
-
0.00017 - 0.00596
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
0.00334 - 0.034
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
0.004 - 0.0334
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
0.003 - 0.004
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
0.0008 - 0.0045
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
0.0002
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme; pH 8.0, 37C, recombinant enzyme
0.00029 - 0.0106
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
0.0034
benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0023
benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0029 - 0.0033
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
0.0012
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin
21C, pH not specified in the publication
0.00268 - 0.0215
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
0.00036 - 0.0177
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
0.0054
benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide
pH 8.0, 37C
0.002 - 0.012
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
0.0023 - 0.003
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
0.0026
benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0028
Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0019
Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide
0.0011
Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0026
Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0024
Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide
pH 8.0, 37C
0.001
Boc-Leu-Lys-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0022
Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.429
DL-Val-Leu-Arg-paranitroanilide
-
-
0.0047 - 0.0048
Gly-Arg-2-naphthylamide
0.0016 - 0.0022
Gly-Gly-Lys-Arg
0.6
N-Benzoyl-Arg amide
-
-
0.0038
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
21C, pH not specified in the publication
0.00093
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin
21C, pH not specified in the publication
0.0031
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
21C, pH not specified in the publication
0.31 - 0.33
N-Benzyloxycarbonyl-Lys benzyl ester
0.234
N-tosyl-Gly-Pro-Arg-paranitroanilide
-
-
0.09 - 0.302
Nalpha-benzoyl-DL-arginine-4-nitroanilide
0.096 - 0.322
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
0.0513 - 0.058
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
0.0038 - 0.0049
Phe-Arg-2-naphthylamide
0.0027 - 0.003
Phe-Arg-Arg
2.2
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.306 - 1.05
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
0.0017 - 0.0021
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
0.0102 - 0.0135
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
0.0013 - 0.0021
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
0.0012 - 0.0019
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
0.0008 - 0.001
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
0.0012 - 0.0013
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
0.001 - 0.0011
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
0.0021 - 0.003
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
0.003 - 0.0044
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
0.0122 - 0.0143
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
0.0099
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0081
Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide
pH 8.0, 37C
0.23
tosyl-Arg methyl ester
-
-
0.47
tosyl-Lys-methyl ester
0.0002 - 0.0003
Val-Lys-Arg-Arg
additional information
additional information
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