Information on EC 3.4.21.83 - Oligopeptidase B

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.4.21.83
-
RECOMMENDED NAME
GeneOntology No.
Oligopeptidase B
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of -Arg-/-, -Lys-/- bonds in oligopeptides, even when P1' residue is proline
show the reaction diagram
hydrolysis of Arg-+-, Lys-+- bonds in oligopeptides, even when P1' residue is proline, the term-+- depicts the point of cleavage
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
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SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oligopeptidase B
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oligopeptidase B
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oligopeptidase B
Serratia proteamaculans 94
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oligopeptidase B
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oligopeptidase B
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oligopeptidase B
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oligopeptidase B
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OPBTc
Trypanosoma cruzi Brener
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OpdB
Serratia proteamaculans 94
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Protease II
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Proteinase, Escherichia coli alkaline, II
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PSP
Serratia proteamaculans 94
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trypsin-like protease
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trypsin-like protease
Serratia proteamaculans 94
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CAS REGISTRY NUMBER
COMMENTARY
57657-67-7
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Escherichia coli JM83 harboring pPR0II-12
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Manually annotated by BRENDA team
HB101 gene cloned and expressed in JM83
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Manually annotated by BRENDA team
strain ATCC 11303
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-
Manually annotated by BRENDA team
serovar typhimurium, virulent wild-type strain SL1344
SwissProt
Manually annotated by BRENDA team
Serratia proteamaculans 94
strain 94
UniProt
Manually annotated by BRENDA team
Trypanosoma cruzi Brener
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
knock-out of OPB results in the disappearance of the serine protease activity of Leishmania extracts. OPB null parasites show an elevated content of enolase protein. This enolase is enzymatically inactive and associated with the parasite membrane. OPB deletion results in a striking alteration in macrophage responses to Leishmania. Whereas wild type parasites elicited little, if any, response from infected macrophages, OPB deletion parasites induce a massive up-regulation in gene transcription. These OPB deletion parasites display decreased virulence in the murine footpad indection model
physiological function
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oligopeptidase B null mutant parasites grow at a significantly faster rate in vitro, and are as virulent as wild type strains during infection in mice. Parasites are revealed in extra vascular regions showing that OPB is not involved in assisting Trypanosoma brucei parasites to cross microvascular endothelial cells. Null mutants show an increase in discrete cysteine peptidase activities when compared to wild type strains. A significant increase of prolyl oligopeptidase activity is observed in mutant, but no concomitant increase in prolyl oligopeptidase protein levels
physiological function
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overexpression of peptidase makes the bacterial cells specifically less susceptible to several proline-rich antimicrobial peptides known to penetrate into the bacterial cytosol, and its level of activity directly correlates with the degree of resistance
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-ADGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ADGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AEGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AEGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AFGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AFGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGDRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGDR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGERGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGER + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGFRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGFR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRDAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + DAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGREAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + EAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRFAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + FAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRGADQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GADQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRGAEQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAEQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
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?
2-aminobenzoyl-AGGRGAFQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAFQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
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?
2-aminobenzoyl-AGGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
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?
2-aminobenzoyl-AGGRGAHQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAHQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
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?
2-aminobenzoyl-AGGRGAIQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAIQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
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?
2-aminobenzoyl-AGGRGALQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GALQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAMQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAMQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGANQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GANQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAPQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
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-
-
-
?
2-aminobenzoyl-AGGRGAQQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAQQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGASQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GASQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGATQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GATQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAVQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAVQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGAYQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GAYQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGDGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GDGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGEGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GEGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGFGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GFGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGGGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GGGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGHGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GHGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGIGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GIGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGLGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GLGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGMGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GMGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGNGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GNGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGPGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GPGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGQGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GQGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGSGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGRG + Ser-Gly-Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGTGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GTGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRGVGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GVGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRGYGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + GYGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRHAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + HAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRIAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + IAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRLAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + LAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRMAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + MAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRNAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + NAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRPAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + PAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRQAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + QAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRSAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + SAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRTAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + TAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGGRVAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + VAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGGRYAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGGR + YAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGHRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGHR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGIRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGIR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGKRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGKR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGLRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGLR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGMRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGMR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGNRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGNR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGPRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGPR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGQRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGQR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGRRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGRR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGRRRAQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-AGSRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGSR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGTRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGTR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AGVRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGVR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AGYRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AGYR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AHGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AHGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AHKRYSHQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-AIGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AIGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-ALGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ALGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AMGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AMGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-AMRRTISQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-ANGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ANGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
2-aminobenzoyl-APGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AQGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AQGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
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?
2-aminobenzoyl-ASGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ASGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
?
2-aminobenzoyl-ATGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-ATGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AVGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AVGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-AYGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-AYGR + GAGQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-FRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
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-
-
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?
2-aminobenzoyl-FRSSR-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-Phe-Arg + Ser-Ser-Arg-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
?
2-aminobenzoyl-GFSPFRSSRIGIKEEQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-GFSPFR + SSRIGIKEEQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-GFSPFRSSRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-LGMISLMKRPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-LGMISLMK + Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
-
?
2-aminobenzoyl-MISLMKRPQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-MISLMK + Arg-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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-
?
2-aminobenzoyl-RPPGFSPFRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-RPPGFSPFR + Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-RPPGFSPFRSSRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-RPPGFSPFR + Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
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?
2-aminobenzoyl-Thr-Arg Cit-Phe(NO2)-Ser-Leu-NH2 + H2O
?
show the reaction diagram
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?
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-Thr-Cit-Cit-Phe(NO2)-Ser-Leu-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
3-(2-furyl)acryloyl-Tyr-Lys-Arg + H2O
3-(2-furyl)acryloyl-Tyr-Lys + Arg
show the reaction diagram
-
-
?
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide + H2O
acetyl-L-Leu-L-Leu-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide + H2O
acetyl-L-Leu-L-Lys-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
acetyl-Leu-Leu-Arg-4-nitroanilide + H2O
acetyl-Leu-Leu-Arg + 4-nitroaniline
show the reaction diagram
-
-
?
Acetyl-Tyr 4-nitroanilide + H2O
Acetyl-Tyr + 4-nitroaniline
show the reaction diagram
-
-
-
-
adrenocorticotropic hormone + H2O
SYSMEHFR + WGK + WGKPVGK + VYP
show the reaction diagram
-
-
-
?
Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
Arg-Arg-2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
?
Arg-Arg-Arg-OH + H2O
Arg-Arg + Arg
show the reaction diagram
-
-
?
Atrial natriuretic factor + H2O
?
show the reaction diagram
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi, cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor
-
-
?
Atrial natriuretic factor + H2O
?
show the reaction diagram
-
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor
-
-
?
azocasein + H2O
?
show the reaction diagram
-
-
-
-
-
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
Benzoyl-Arg 2-naphthylamide + H2O
Benzoyl-Arg + 2-naphthylamine
show the reaction diagram
-
-
-
-
benzoyl-Arg-2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzoyl-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzoyl-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzoyl-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzoyl-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzoyl-Arg-beta-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-Et + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-NH2 + H2O
?
show the reaction diagram
-
-
-
-
-
benzoyl-Arg-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-arginine ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-citrulline-Et + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Gly-Gly-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzoyl-L-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzoyl-L-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O
benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
Benzoyl-Lys 2-naphthylamide + H2O
Benzoyl-Lys + 2-naphthylamine
show the reaction diagram
-
-
-
-
Benzoyl-Lys 2-naphthylamide + H2O
Benzoyl-Lys + 2-naphthylamine
show the reaction diagram
-
-
-
-
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzoyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Trypanosoma cruzi, Trypanosoma cruzi Brener
-
-
-
?
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl 7-amide+ H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Arg-Arg-7-(4-methylcoumaryl)amide + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Gly-L-Arg-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-L-Leu-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Leu-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Val-L-Val-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amino-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
Boc-Leu-Lys-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
CBZ-L-Arg-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
weak substrate
-
?
Denatured casein + H2O
?
show the reaction diagram
-
weak
-
-
-
DL-Val-Leu-Arg-paranitroanilide + H2O
DL-Val-Leu-Arg + p-nitroaniline
show the reaction diagram
-
-
-
?
Glucagon + H2O
?
show the reaction diagram
-
wild type enzyme cleaves Lys12-Tyr13, Arg17-Arg18, and Arg18-Ala19 bonds of glucagon
-
-
?
Gly-Arg-2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
?
Gly-Gly-Lys-Arg + H2O
Gly-Gly-Lys-Arg + Arg
show the reaction diagram
-
-
?
histone + H2O
?
show the reaction diagram
human histones H1, H2A, H3 and H4, restricted proteolysis
-
-
?
Insulin B-chain + H2O
?
show the reaction diagram
-
cleaves the carboxymethylated B-chain of insulin at the Arg22-Gly23 bond, but after prolonged periods of incubation it is also able to cleave the Tyr16-Leu17 bond
-
-
-
Insulin B-chain + H2O
?
show the reaction diagram
-
cleavage at the carboxyl sides of Arg and Lys residues
-
-
-
L-pGlu-Gly-L-Arg-7-amido-4-methylcoumarin + H2O
L-pGlu-Gly-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low hydrolytic rate
-
?
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin + H2O
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low hydrolytic rate
-
?
L-Phe-L-Arg 2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
-
N-Benzoyl-DL-Arg 4-nitroanilide + H2O
N-Benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
N-Benzoyl-DL-Arg 4-nitroanilide + H2O
N-Benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
N-Benzoyl-L-Arg ethyl ester + H2O
N-Benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
-
-
N-Benzoyl-L-Arg ethyl ester + H2O
N-Benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
-
-
N-benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
N-Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O
N-Benzyloxycarbonyl-Gly + 4-nitrophenol
show the reaction diagram
-
weak
-
-
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Gly-Gly-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
N-Benzyloxycarbonyl-Lys benzyl ester + H2O
N-Benzyloxycarbonyl-Lys + benzol
show the reaction diagram
-
-
-
-
N-Carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O
N-Carbobenzoxy-L-Lys + 4-nitrophenol
show the reaction diagram
-
-
-
-
N-Carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O
N-Carbobenzoxy-L-Lys + 4-nitrophenol
show the reaction diagram
-
-
-
-
N-tosyl-Gly-Pro-Arg-paranitroanilide + H2O
N-tosyl-Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
?
Nalpha-benzoyl-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
68% of the activity with carbobenzoxy-Arg-Arg-4-methylcoumaryl-7-amide
-
-
?
Nalpha-benzoyl-DL-Arg-4-nitroanilide + H2O
Nalpha-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
?
Nalpha-benzoyl-DL-Arg-4-nitroanilide + H2O
Nalpha-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
Serratia proteamaculans, Serratia proteamaculans 94
-
-
?
Nalpha-benzyloxycarbonyl-L-Ala-L-Ala-L-Arg-4-nitroanilide + H2O
Nalpha-benzyloxycarbonyl-L-Ala-L-Ala-L-Arg + 4-nitroaniline
show the reaction diagram
-
poor substrate
-
?
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester + H2O
Nalpha-benzyloxycarbonyl-L-lysine + phenylmethanethiol
show the reaction diagram
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide + H2O
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
Phe-Arg-2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
?
Phe-Arg-Arg + H2O
Phe-Arg + Arg
show the reaction diagram
-
-
?
RRRPRPPRLPRPRPR + 2 H2O
RR + RPRPPR + LPRPRPR
show the reaction diagram
-
proline-rich peptide Bac(1-16)
-
?
RRRPRPPYLPRPRPPPFF + 2 H2O
RR + RPRPPYLPRPRPPPFF
show the reaction diagram
-
proline-rich peptide PR-39(1-18)
-
?
succinyl-Gly-L-Pro-L-Lys-4-nitroanilide + H2O
succinyl-Gly-L-Pro-L-Lys + 4-nitroaniline
show the reaction diagram
-
poor substrate
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 4-nitroaniline
show the reaction diagram
-
-
-
?
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
best substrate, the hydrolytic activity is observed only in the presence of NaCl and the thiol-reacting reagents dithiothreitol, reduced glutathione, and oxidized glutathione at pH 8.5 (50 mM Tris-HCl)
-
?
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
specific substrate for Escherichia coli oligopeptidase B, 20% of the activity with carbobenzoxy-Arg-Arg-4-methylcoumaryl-7-amide
-
-
?
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
?
Tosyl-Arg methyl ester + H2O
Tosyl-Arg + methanol
show the reaction diagram
-
-
-
-
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
tosyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
tosyl-Gly-L-Pro-L-Lys-7-amido-4-methylcoumarin + H2O
tosyl-Gly-L-Pro-L-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Tosyl-Lys methyl ester + H2O
Tosyl-Lys + benzyl alcohol
show the reaction diagram
-
-
-
-
Tosyl-Lys methyl ester + H2O
Tosyl-Lys + benzyl alcohol
show the reaction diagram
-
-
-
-
Val-Lys-Arg-Arg + H2O
Val-Lys-Arg + Arg
show the reaction diagram
-
-
?
L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
L-Pro-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
additional information
?
-
-
not: luteinizing hormone releasing factor
-
-
-
additional information
?
-
exhibits amidolytic activity exclusively against substrates with basic residues in P1, no activity is observed against H-Arg-4-methylcoumarin 7-amide, H-Leu-4-methylcoumarin 7-amide, H-Lys-4-methylcoumarin 7-amide, benzyloxycarbonyl-Leu-Leu-Glu-beta-nitroanilide, benzyloxycarbonyl-Gly-Gly-Leu-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-4-methylcoumarin 7-amide, Suc-Ile-Ala-4-methylcoumarin 7-amide, Suc-Gly-Pro-4-methylcoumarin 7-amide, Ac-Tyr-Val-Ala-Asp-p-nitroanilide, Suc-Ala-Ala-Pro-Phe-4-methylcoumarin 7-amide
-
-
?
additional information
?
-
-
hydrolyzes peptides with dibasic sites much faster than monobasic substrates, hydrolyzes the peptide bond at lysine and arginine residues
-
-
?
additional information
?
-
-
no hydrolysis of 2-aminobenzoyl acid-LGMISLMKRPPGFSPFRSSRI-NH2-N-(2,4-dinitrophenyl)ethylenediamine
-
-
?
additional information
?
-
-
specific for substrates with a pair of basic residues at positions P1 and P2, involved in host cell invasion
-
-
?
additional information
?
-
-
the enzyme may be associated with Chagas disease pathogenesis nb yhydrolyzing host proteins and inducing host immune responses
-
-
-
additional information
?
-
cleaves oxidised insulin B chain, OpdB does not hydrolyse protein substrates with the exception of nominal digestion of the endogenous Escherichia coli enzymes aspartokinase L-homoserine dehydrogenase and aspartokinase III
-
-
-
additional information
?
-
-
no hydrolytic activity against the aminopeptidase substrates (4-nitroanilide derivatives of amino acids) and FITC-casein, substrates containing Pro at Xaa positions in the FRETS library are not hydrolyzed
-
-
-
additional information
?
-
no substrate: N-benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin
-
-
-
additional information
?
-
OPB has a strong preference for lysine or arginine residues at P1. OPB can accommodate several amino acids in the P2P4 positions, with a preference for glycine in P2, serine, glycine, alanine, asparagine, proline, and threonine in P3, and proline in P4. Bulky hydrophobic groups, such as tyrosine, phenylalanine, and tryptophan, are least preferred
-
-
-
additional information
?
-
-
oligopeptidase B can efficiently hydrolyze in vitro cationic antimicrobial peptides up to 30 residues in length, even though they contained several prolines, shortening them to inactive fragments. Two consecutive basic residues are a preferred cleavage site for the peptidase. In the case of a single basic residue, there is no cleavage if proline residues are present in the P1 and P2 positions
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Atrial natriuretic factor + H2O
?
show the reaction diagram
Q6QDP1
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi
-
-
?
additional information
?
-
-
involved in host cell invasion
-
-
?
additional information
?
-
-
the enzyme may be associated with Chagas disease pathogenesis nb yhydrolyzing host proteins and inducing host immune responses
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
stimulates amidase and proteolytic activities, maximal activation of 25% at concentrations above 25 mM
Ca2+
-
activation at 5-10 mM CaCl2
Ca2+
-
in presence of Ca2+, efficiency of hydrolysis of substrates with positively charged P2 residues decreases by an order of magnitude due to the worsening in the substrate binding. In the presence of 50 mM Ca2+, no substrate inhibition is observed in the case of acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide, and the effect was less pronounced for other substrates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3,4-dichloroisocoumarin
irreversible inhibition
3,4-dichloroisocoumarin
irreversible
3,4-dichloroisocoumarin
non-peptide irreversible serine-peptidase inhibitor
4-(2-aminoethyl)benzenesulfonyl fluoride
irreversible inhibition
4-(2-aminoethyl)benzenesulfonyl fluoride
irreversible
4-(2-aminoethyl)benzenesulfonyl fluoride
-
OpdB activity toward tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin hydrolysis is inhibited completely by 4 mM pefbloc SC
4-(2-aminoethyl)benzenesulfonylfluoride
non-peptide irreversible serine-peptidase inhibitor
4-methylumbelliferyl-p-guanidobenzoate
active site titrant
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
-
acetyl-Thr-Arg-Arg
-
-
agmatine
reversible
antipain
reversible inhibition
antipain
reversible
antipain
reversible competitive inhibitor
antipain
-
OpdB activity toward tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin hydrolysis is inhibited completely by 0.3 mM antipain
antipain
0.1 mM, no residual activity
antipain dihydrochloride
-
1 mM, 79% remaining activity
Aromatic amidines
-
-
-
benzamidine
-
-
benzamidine
reversible inhibition
benzamidine
reversible
benzamidine
1 mM, 27% residual activity
benzoyl-Arg-4-nitroanilide
-
enzyme is inhibited by high concentrations of substrate
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
-
bovine basic pancreatic trypsin inhibitor
-
substrate Nalpha-benzoyl-DL-Arg-4-nitroanilide, competitive
-
Ca2+
0.01 mM, 69% residual activity
chymostatin
-
0,1 mM, 45% inhibition
diisopropylfluorophosphate
non-peptide irreversible serine-peptidase inhibitor
EDTA
-
1.3 mM, 98% remaining activity, 20 mM, 57% remaining activity
iodoacetamide
-
-
iodoacetic acid
-
L-arginine
-
-
Leupeptin
-
weak
Leupeptin
reversible inhibition
Leupeptin
reversible
Leupeptin
reversible competitive inhibitor
Leupeptin
-
OpdB activity toward tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin hydrolysis is inhibited completely by 0.05 mM leupeptin
Leupeptin
0.1 mM, no residual activity
m-phenanthroline
-
substrates Nalpha-benzoyl-DL-Arg-4-nitroanilide, acetyl-Leu-Leu-Arg-4-nitroanilide, anticompetitive
methanol
incubation in 20% (v/v) methanol (10-20 h, 4C) results in partial decomposition of the high molecular weight PSP-GroEL complex with precipitation of denatured chaperonin and a small (no more than 10%) loss of PSP activity
Mg2+
0.01 mM, 73% residual activity
N-ethylmaleimide
-
N-ethylmaleimide
-
-
NaCl
-
loss of almost 50 and 70% of its activity in the presence of 0.2 and 1.0 M NaCl, respectively. Presence of NaCl does not disrupt the dimeric structure
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
-
NEM
-
Cys256 is the reactive cysteine residue that mediates OpdB inhibition. Cys256 adducts occlude the P1 substrate-binding site, preventing substrate binding
o-phenanthroline
-
substrates Nalpha-benzoyl-DL-Arg-4-nitroanilide, acetyl-Leu-Leu-Arg-4-nitroanilide, anticompetitive
p-aminobenzamidine
-
-
p-aminobenzamidine
reversible inhibition
p-chloromercuribenzoate
non-peptide irreversible serine-peptidase inhibitor
Pefabloc SC
-
4 mM, 93% inhibition
Pefabloc SC
2 mM, no residual activity
Pentamidine
reversible competitive inhibitor
Pepstatin
-
1 microM, 94% remaining activity
peptidyl chloromethyl ketone
irreversible peptidyl inhibitors
peptidyl diazomethyl ketone
irreversible peptidyl inhibitors
peptidyl phosphonate alpha-aminoalkyl diphenyl ester
irreversible peptidyl inhibitors
Phe-Pro-Arg-chloromethylketone
0.01 mM, no residual activity
phenylmethane sulfonyl fluoride
irreversible inhibition
phenylmethanesulfonyl fluoride
irreversible
phosphoramidon
-
0.57 mM, 96% remaining activity
protamine
reversible competitive inhibitor
protamines
-
protamines, basic 30-32 residue peptides that are rich in Arg residues, are potent inhibitors of OpdB
-
suramin
partial non-competitive inhibitor
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin
-
in the case of added dithiothreitol, reduced glutathione, or oxidized glutathione, a high concentration of the substrate tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin inhibits the enzyme activity. By contrast, addition of 1 M NaCl to either dithiothreitol, reduced glutathione, or oxidized glutathione or 1 M NaCl alone prevents substrate inhibition
Tos-LysCH2Cl
non-peptide irreversible serine-peptidase inhibitor
tosyl-L-Lys-chloromethylketone
0.1 mM, no residual activity
tosyl-Leu chloromethyl ketone
-
-
trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester
-
IC50: about 0.01 mM. Oligopeptidase B is much more sensitive than oligopeptidase B
Zn2+
0.01 mM, no residual activity
ZnCl2
-
substrate Nalpha-benzoyl-DL-Arg-4-nitroanilide, noncompetitive
Mn2+
0.01 mM, 73% residual activity
additional information
-
1,10-phenanthroline; EDTA; not: chelating agents; PMSF; several natural trypsin inhibitors; sulfhydryl agents
-
additional information
-
several natural trypsin inhibitors
-
additional information
no inhibition of wild-type enzyme with iodoacetamide, iodoacetate, N-ethylmaleimide, EDTA, EGTA, bestatin,amastatin, arphamenine A, elastinal, dynorphin A or dynorphin B
-
additional information
-
no inhibition by N-ethylmaleimide and iodoacetic acid. This is consistent with the absence of a cysteine residue at position 256 which is replaced by His
-
additional information
-
OpdB is not inhibited by E-64 (0.03 mM), pepstain (0.04 mM), EDTA (1 mM), chimostatin (0.2 mM), and phosphoramidon (0.4 mM)
-
additional information
-
reducing reagent such as dithiothreitol has no effect on the inactivation of wild type and mutant OPBs by heat and urea treatment
-
additional information
-
dithiothreitol is not inhibitoryup to 40 mM
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Aprotinin
-
0.3 microM, 103% activity
bestatin
-
0.13 mM, 105% activity
dithiothreitol
-
dithiothreitol
-
enzymatic activity is markedly enhanced by dithiothreitol at submillimolar level, which affects the P1 preference of OpdB synergistically in a substrate-dependent manner
E-64
-
5 microM, 114% activity
Leupeptin
-
1 microM, 116% activity
NaCl
-
enzymatic activity is markedly enhanced by high NaCl concentration, NaCl affects the P1 preference of OpdB synergistically in a substrate-dependent manner
oxidized glutathione
-
oxidized glutathione enhances OpdB activity and affects the P1 preference of OpdB synergistically in a substrate-dependent manner
reduced glutathione
-
enzymatic activity is markedly enhanced by reduced glutathione at submillimolar level which affects the P1 preference of OpdB synergistically in a substrate-dependent manner
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0036
2-aminobenzoyl-ADGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.039
2-aminobenzoyl-AEGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0032
2-aminobenzoyl-AFGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0034
2-aminobenzoyl-AFGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.013
2-aminobenzoyl-AGDRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0033
2-aminobenzoyl-AGERGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0024
2-aminobenzoyl-AGFRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0031
2-aminobenzoyl-AGFRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0015
2-aminobenzoyl-AGGRDAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0045
2-aminobenzoyl-AGGRDAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0014
2-aminobenzoyl-AGGREAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0065
2-aminobenzoyl-AGGREAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0003
2-aminobenzoyl-AGGRFAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0027
2-aminobenzoyl-AGGRFAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0057
2-aminobenzoyl-AGGRGADQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.021
2-aminobenzoyl-AGGRGAEQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0062
2-aminobenzoyl-AGGRGAFQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0097
2-aminobenzoyl-AGGRGAFQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0011
2-aminobenzoyl-AGGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0072
2-aminobenzoyl-AGGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0034
2-aminobenzoyl-AGGRGAHQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0037
2-aminobenzoyl-AGGRGAHQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.011
2-aminobenzoyl-AGGRGAIQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.031
2-aminobenzoyl-AGGRGAIQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0073
2-aminobenzoyl-AGGRGALQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.012
2-aminobenzoyl-AGGRGALQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.003
2-aminobenzoyl-AGGRGAMQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0031
2-aminobenzoyl-AGGRGAMQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0059
2-aminobenzoyl-AGGRGANQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.018
2-aminobenzoyl-AGGRGANQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0011
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0028
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0072
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.013
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0048
2-aminobenzoyl-AGGRGAQQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0088
2-aminobenzoyl-AGGRGAQQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.002
2-aminobenzoyl-AGGRGASQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.012
2-aminobenzoyl-AGGRGASQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.006
2-aminobenzoyl-AGGRGATQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.027
2-aminobenzoyl-AGGRGATQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0091
2-aminobenzoyl-AGGRGAVQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.021
2-aminobenzoyl-AGGRGAVQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0057
2-aminobenzoyl-AGGRGAYQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.011
2-aminobenzoyl-AGGRGAYQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.008
2-aminobenzoyl-AGGRGDGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.012
2-aminobenzoyl-AGGRGDGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.01
2-aminobenzoyl-AGGRGEGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.138
2-aminobenzoyl-AGGRGEGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0021
2-aminobenzoyl-AGGRGFGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.012
2-aminobenzoyl-AGGRGFGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0062
2-aminobenzoyl-AGGRGGGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.014
2-aminobenzoyl-AGGRGGGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0028
2-aminobenzoyl-AGGRGHGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0033
2-aminobenzoyl-AGGRGHGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0032
2-aminobenzoyl-AGGRGIGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.011
2-aminobenzoyl-AGGRGIGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0052
2-aminobenzoyl-AGGRGLGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.024
2-aminobenzoyl-AGGRGLGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.003
2-aminobenzoyl-AGGRGMGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.02
2-aminobenzoyl-AGGRGMGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0046
2-aminobenzoyl-AGGRGNGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0092
2-aminobenzoyl-AGGRGNGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0012
2-aminobenzoyl-AGGRGPGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0098
2-aminobenzoyl-AGGRGPGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0023
2-aminobenzoyl-AGGRGQGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.01
2-aminobenzoyl-AGGRGQGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0032
2-aminobenzoyl-AGGRGSGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0037
2-aminobenzoyl-AGGRGSGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0058
2-aminobenzoyl-AGGRGTGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.013
2-aminobenzoyl-AGGRGTGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.02
2-aminobenzoyl-AGGRGVGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.031
2-aminobenzoyl-AGGRGVGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0039
2-aminobenzoyl-AGGRGYGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0044
2-aminobenzoyl-AGGRGYGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0005
2-aminobenzoyl-AGGRHAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0017
2-aminobenzoyl-AGGRHAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0003
2-aminobenzoyl-AGGRIAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0005
2-aminobenzoyl-AGGRIAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0006
2-aminobenzoyl-AGGRLAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0013
2-aminobenzoyl-AGGRLAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0069
2-aminobenzoyl-AGGRMAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0083
2-aminobenzoyl-AGGRMAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0004
2-aminobenzoyl-AGGRNAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0013
2-aminobenzoyl-AGGRNAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0005
2-aminobenzoyl-AGGRQAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0007
2-aminobenzoyl-AGGRQAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0005
2-aminobenzoyl-AGGRSAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0012
2-aminobenzoyl-AGGRSAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0003
2-aminobenzoyl-AGGRTAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0009
2-aminobenzoyl-AGGRTAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0001
2-aminobenzoyl-AGGRVAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0014
2-aminobenzoyl-AGGRVAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0004
2-aminobenzoyl-AGGRYAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0006
2-aminobenzoyl-AGGRYAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0063
2-aminobenzoyl-AGHRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0012
2-aminobenzoyl-AGIRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.01
2-aminobenzoyl-AGIRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0018
2-aminobenzoyl-AGKRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0048
2-aminobenzoyl-AGKRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0019
2-aminobenzoyl-AGLRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0077
2-aminobenzoyl-AGLRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0025
2-aminobenzoyl-AGMRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0029
2-aminobenzoyl-AGMRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.004
2-aminobenzoyl-AGNRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.023
2-aminobenzoyl-AGNRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0082
2-aminobenzoyl-AGPRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.016
2-aminobenzoyl-AGPRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0014
2-aminobenzoyl-AGQRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0059
2-aminobenzoyl-AGQRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0004
2-aminobenzoyl-AGSRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0006
2-aminobenzoyl-AGSRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0019
2-aminobenzoyl-AGTRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0075
2-aminobenzoyl-AGTRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0014
2-aminobenzoyl-AGVRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0039
2-aminobenzoyl-AGVRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0077
2-aminobenzoyl-AGYRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.014
2-aminobenzoyl-AGYRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0032
2-aminobenzoyl-AHGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0035
2-aminobenzoyl-AHGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.002
2-aminobenzoyl-AIGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0044
2-aminobenzoyl-AIGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0013
2-aminobenzoyl-ALGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0026
2-aminobenzoyl-ALGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0009
2-aminobenzoyl-AMGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH8.0, 37C
0.0059
2-aminobenzoyl-AMGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0083
2-aminobenzoyl-ANGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.001
2-aminobenzoyl-APGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH8.0, 37C
0.0019
2-aminobenzoyl-APGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0019
2-aminobenzoyl-AQGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0024
2-aminobenzoyl-AQGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0023
2-aminobenzoyl-ASGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0074
2-aminobenzoyl-ASGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0018
2-aminobenzoyl-ATGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0082
2-aminobenzoyl-ATGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0026
2-aminobenzoyl-AVGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.013
2-aminobenzoyl-AVGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0018
2-aminobenzoyl-AYGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.003
2-aminobenzoyl-AYGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
-
pH 8.0, 37C
0.0298
2-aminobenzoyl-Thr-Arg Cit-Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C
0.0048
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C, 3 mM
0.014
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 15C, 1 M
0.024
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C
0.0379
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 35C
0.083
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C
0.08
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2
-
pH 8.0, 25C, 1 M
0.0011
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37C, native enzyme
0.0019
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37C, recombinant enzyme
0.224
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0.457
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.0226
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0.265
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
80
acetyl-tyrosine 4-nitroanilide
-
-
0.0044
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.005
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.04
Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.048
Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.002
Arg-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
0.0024
Arg-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
0.0006
Arg-Arg-Arg
pH 8.0, 37C, native enzyme
0.0011
Arg-Arg-Arg
pH 8.0, 37C, recombinant enzyme
0.25
Benzoyl-Arg 2-naphthylamide
-
-
0.5
benzoyl-Arg 4-nitroanilide
-
-
0.5
benzoyl-Arg 4-nitroanilide
-
benzoyl-Arg ethyl ester
0.48
benzoyl-Arg ethyl ester
-
-
0.195
benzoyl-Arg-4-nitroanilide
-
pH 7.10, 25C
0.296
benzoyl-Arg-4-nitroanilide
-
pH 8.03, 25C
0.301
benzoyl-Arg-4-nitroanilide
-
pH 8.00, 25C
1.24
benzoyl-Arg-4-nitroanilide
-
pH 8.10, 25C
0.00268
benzoyl-Arg-7-amido-4-methylcoumarin
-
wild-type, pH not specified in the publication, temperature not specified in the publication
-
0.00564
benzoyl-Arg-7-amido-4-methylcoumarin
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
-
0.00846
benzoyl-Arg-7-amido-4-methylcoumarin
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
-
0.0212
benzoyl-Arg-7-amido-4-methylcoumarin
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
-
0.0411
benzoyl-Arg-7-amido-4-methylcoumarin
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
-
0.097
benzoyl-Arg-beta-naphthylamide
-
pH 8.00, 25C
0.00308
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
pH 8.0, 25C
0.00776
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
pH 8.0, 25C
0.00334
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
wild type enzyme, at 37C
0.0044
benzoyl-L-Arg-7-amido-4-methylcoumarin
21C, pH not specified in the publication
0.00528
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q, at 37C
0.00568
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E610Q, at 37C
0.00904
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609L, at 37C
0.00913
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609F, at 37C
0.0101
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609A, at 37C
0.0113
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q, at 37C
0.0127
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q/E609Q, at 37C
0.0173
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q/E610Q, at 37C
0.0184
benzoyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609R, at 37C
0.084
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.54
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0.92
Benzoyl-Lys 2-naphthylamide
-
-
0.00017
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
0.00027
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.00043
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
0.00493
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
0.00596
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
0.00334
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
wild-type, pH not specified in the publication, temperature not specified in the publication
-
0.00421
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
-
0.0108
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
-
0.0185
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
-
0.034
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
-
0.004
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D638Q
0.0044
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0045
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D599H
0.0046
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D460T and E494H
0.0047
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D462N
0.005
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D567H
0.0051
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, opdB with polyhistidine affinty tag removed
0.0052
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D460T/D462N
0.0054
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant E624H
0.0164
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant E578A
0.0334
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant E576A
0.003
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.004
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.0008
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, wild-type and mutant D567H
0.00088
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, opdB with polyhistidine affinty tag removed
0.001
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant E494H, E624H, and D599H
0.0011
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D638Q
0.0021
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D460T
0.0023
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D462N
0.0029
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D460T/D462N
0.0045
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant E576A
0.0002
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme; pH 8.0, 37C, recombinant enzyme
0.00029
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
-
0.00063
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
-
wild-type, pH not specified in the publication, temperature not specified in the publication
-
0.00078
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
-
0.0011
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
-
0.0106
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
-
0.0034
benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0023
benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0029
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.0033
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.0012
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin
21C, pH not specified in the publication
0.00268
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
wild type enzyme, at 37C
0.00525
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609A, at 37C
0.00586
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E610Q, at 37C
0.00637
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609L, at 37C
0.0069
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609F, at 37C
0.00728
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q/E609Q, at 37C
0.00807
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q, at 37C
0.0127
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609Q/E610Q, at 37C
0.015
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E607Q, at 37C
0.0215
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
-
mutant enzyme E609R, at 37C
0.00036
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
-
0.00117
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
-
wild-type, pH not specified in the publication, temperature not specified in the publication
-
0.00365
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
-
0.00856
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
-
0.0177
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
-
0.0054
benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide
pH 8.0, 37C
0.002
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D567H
0.0021
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, wild-type and mutant D599A; pH 8.0, 37C, wild-type and mutant D599H
0.0025
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant E494H
0.0026
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant E624H
0.0028
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D638Q
0.0029
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D460T and D460T/D462N
0.003
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant D462N
0.0059
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant E578A
0.012
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C, mutant E576A
0.0023
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.003
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.0026
benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0028
Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0019
Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0019
Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0011
Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0026
Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0024
Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide
pH 8.0, 37C
0.001
Boc-Leu-Lys-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0022
Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.429
DL-Val-Leu-Arg-paranitroanilide
-
-
0.0047
Gly-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
0.0048
Gly-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
0.0016
Gly-Gly-Lys-Arg
pH 8.0, 37C, native enzyme
0.6
N-Benzoyl-Arg amide
-
-
0.0038
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
21C, pH not specified in the publication
0.00093
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin
21C, pH not specified in the publication
0.0031
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
21C, pH not specified in the publication
0.31
N-Benzyloxycarbonyl-Lys benzyl ester
-
-
0.33
N-Benzyloxycarbonyl-Lys benzyl ester
-
-
0.234
N-tosyl-Gly-Pro-Arg-paranitroanilide
-
-
0.09
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
pH 8.0, 25C
0.302
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.096
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
-
pH 8.0, 25C
0.322
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.0513
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.058
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0.0038
Phe-Arg-2-naphthylamide
pH 8.0, 37C, native enzyme
0.0049
Phe-Arg-2-naphthylamide
pH 8.0, 37C, recombinant enzyme
0.0027
Phe-Arg-Arg
pH 8.0, 37C, native enzyme
0.003
Phe-Arg-Arg
pH 8.0, 37C, recombinant enzyme
2.2
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.306
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
1.05
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0.0017
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.0021
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.0102
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.0135
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.0013
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.0021
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.0012
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.0019
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.0008
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.001
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.0012
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.0013
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.001
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.0011
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.0021
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.003
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.003
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.0044
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.0122
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, native enzyme
0.0143
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37C, recombinant enzyme
0.0099
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide
pH 8.0, 37C
0.0081
Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide
pH 8.0, 37C
0.23
tosyl-Arg methyl ester
-
-
0.47
tosyl-Lys-methyl ester
-
N-benzoyl-L-Arg ethyl ester
0.47
tosyl-Lys-methyl ester
-
-
0.0002
Val-Lys-Arg-Arg
pH 8.0, 37C, native enzyme
0.0003
Val-Lys-Arg-Arg
pH 8.0, 37C, recombinant enzyme
0.0022
Gly-Gly-Lys-Arg
pH 8.0, 37C, recombinant enzyme
additional information
additional information
-
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
288
2-aminobenzoyl-ADGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
312
2-aminobenzoyl-AEGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
312
2-aminobenzoyl-AFGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
2200
2-aminobenzoyl-AFGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
180
2-aminobenzoyl-AGDRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
108
2-aminobenzoyl-AGERGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
582
2-aminobenzoyl-AGFRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1320
2-aminobenzoyl-AGFRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
900
2-aminobenzoyl-AGGRDAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei, Trypanosoma cruzi
-
pH 8.0, 37C
780
2-aminobenzoyl-AGGREAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
900
2-aminobenzoyl-AGGREAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
660
2-aminobenzoyl-AGGRFAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1440
2-aminobenzoyl-AGGRFAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1020
2-aminobenzoyl-AGGRGADQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
402
2-aminobenzoyl-AGGRGAEQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
900
2-aminobenzoyl-AGGRGAFQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
4080
2-aminobenzoyl-AGGRGAFQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
312
2-aminobenzoyl-AGGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
354
2-aminobenzoyl-AGGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
840
2-aminobenzoyl-AGGRGAHQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1500
2-aminobenzoyl-AGGRGAHQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
414
2-aminobenzoyl-AGGRGAIQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
3300
2-aminobenzoyl-AGGRGAIQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1380
2-aminobenzoyl-AGGRGALQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
2820
2-aminobenzoyl-AGGRGALQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
900
2-aminobenzoyl-AGGRGAMQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1200
2-aminobenzoyl-AGGRGAMQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1020
2-aminobenzoyl-AGGRGANQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1860
2-aminobenzoyl-AGGRGANQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
108
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
186
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
312
2-aminobenzoyl-AGGRGAPQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
390
2-aminobenzoyl-AGGRGAQQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
3060
2-aminobenzoyl-AGGRGAQQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
564
2-aminobenzoyl-AGGRGASQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1620
2-aminobenzoyl-AGGRGASQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
2220
2-aminobenzoyl-AGGRGATQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
2880
2-aminobenzoyl-AGGRGATQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
2160
2-aminobenzoyl-AGGRGAVQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
2640
2-aminobenzoyl-AGGRGAVQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1200
2-aminobenzoyl-AGGRGAYQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
2760
2-aminobenzoyl-AGGRGAYQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
264
2-aminobenzoyl-AGGRGDGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
720
2-aminobenzoyl-AGGRGDGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
720
2-aminobenzoyl-AGGRGEGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1980
2-aminobenzoyl-AGGRGEGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
900
2-aminobenzoyl-AGGRGFGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
2160
2-aminobenzoyl-AGGRGFGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
660
2-aminobenzoyl-AGGRGGGQG-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
780
2-aminobenzoyl-AGGRGGGQG-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
960
2-aminobenzoyl-AGGRGHGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1020
2-aminobenzoyl-AGGRGHGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1680
2-aminobenzoyl-AGGRGIGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1740
2-aminobenzoyl-AGGRGIGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1500
2-aminobenzoyl-AGGRGLGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
3720
2-aminobenzoyl-AGGRGLGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
660
2-aminobenzoyl-AGGRGMGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
3240
2-aminobenzoyl-AGGRGMGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
432
2-aminobenzoyl-AGGRGNGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
660
2-aminobenzoyl-AGGRGNGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
336
2-aminobenzoyl-AGGRGPGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
450
2-aminobenzoyl-AGGRGPGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
552
2-aminobenzoyl-AGGRGQGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
840
2-aminobenzoyl-AGGRGQGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
588
2-aminobenzoyl-AGGRGSGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1380
2-aminobenzoyl-AGGRGSGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1020
2-aminobenzoyl-AGGRGTGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1140
2-aminobenzoyl-AGGRGTGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
2400
2-aminobenzoyl-AGGRGVGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
3180
2-aminobenzoyl-AGGRGVGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1200
2-aminobenzoyl-AGGRGYGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1380
2-aminobenzoyl-AGGRGYGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
552
2-aminobenzoyl-AGGRHAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1620
2-aminobenzoyl-AGGRHAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
186
2-aminobenzoyl-AGGRIAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
240
2-aminobenzoyl-AGGRIAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
246
2-aminobenzoyl-AGGRLAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
360
2-aminobenzoyl-AGGRLAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1020
2-aminobenzoyl-AGGRMAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1140
2-aminobenzoyl-AGGRMAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
840
2-aminobenzoyl-AGGRNAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei, Trypanosoma cruzi
-
pH 8.0, 37C
660
2-aminobenzoyl-AGGRQAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
720
2-aminobenzoyl-AGGRQAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
720
2-aminobenzoyl-AGGRSAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1260
2-aminobenzoyl-AGGRSAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
660
2-aminobenzoyl-AGGRTAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
720
2-aminobenzoyl-AGGRTAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
156
2-aminobenzoyl-AGGRVAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
288
2-aminobenzoyl-AGGRVAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
660
2-aminobenzoyl-AGGRYAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
780
2-aminobenzoyl-AGGRYAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
720
2-aminobenzoyl-AGHRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
396
2-aminobenzoyl-AGIRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
780
2-aminobenzoyl-AGIRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
264
2-aminobenzoyl-AGKRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
444
2-aminobenzoyl-AGKRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
900
2-aminobenzoyl-AGLRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1140
2-aminobenzoyl-AGLRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
780
2-aminobenzoyl-AGMRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1440
2-aminobenzoyl-AGMRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
426
2-aminobenzoyl-AGNRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
468
2-aminobenzoyl-AGNRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
216
2-aminobenzoyl-AGPRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
720
2-aminobenzoyl-AGPRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
600
2-aminobenzoyl-AGQRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
960
2-aminobenzoyl-AGQRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
3 - 6
2-aminobenzoyl-AGSRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
102
2-aminobenzoyl-AGSRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1140
2-aminobenzoyl-AGTRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1620
2-aminobenzoyl-AGTRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
84
2-aminobenzoyl-AGVRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
294
2-aminobenzoyl-AGVRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
720
2-aminobenzoyl-AGYRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1440
2-aminobenzoyl-AGYRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1140
2-aminobenzoyl-AHGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1260
2-aminobenzoyl-AHGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1380
2-aminobenzoyl-AIGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
2100
2-aminobenzoyl-AIGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
840
2-aminobenzoyl-ALGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
1740
2-aminobenzoyl-ALGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1380
2-aminobenzoyl-AMGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
2340
2-aminobenzoyl-AMGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
450
2-aminobenzoyl-ANGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
132
2-aminobenzoyl-APGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
264
2-aminobenzoyl-APGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
780
2-aminobenzoyl-AQGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
2520
2-aminobenzoyl-AQGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1020
2-aminobenzoyl-ASGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
2460
2-aminobenzoyl-ASGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
1800
2-aminobenzoyl-ATGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei, Trypanosoma cruzi
-
pH 8.0, 37C
186
2-aminobenzoyl-AVGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
2700
2-aminobenzoyl-AVGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
486
2-aminobenzoyl-AYGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma cruzi
-
pH 8.0, 37C
2820
2-aminobenzoyl-AYGRGAGQ-N-(2,4-dinitrophenyl)ethylenediamine
Trypanosoma brucei
-
pH 8.0, 37C
294
2-aminobenzoyl-Thr-Arg Cit-Phe(NO2)-Ser-Leu-NH2
Escherichia coli
-
pH 8.0, 25C
516
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
Escherichia coli
-
pH 8.0, 15C, 1 M
1260
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
Escherichia coli
-
pH 8.0, 25C, 3 mM
1390
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
Escherichia coli
-
pH 8.0, 25C
1392
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
Escherichia coli
-
pH 8.0, 25C
1416
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
Escherichia coli
-
pH 8.0, 35C
1420
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2
Escherichia coli
-
pH 8.0, 35C
1218
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2
Escherichia coli
-
pH 8.0, 25C
1220
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2
Escherichia coli
-
pH 8.0, 25C
12
3-(2-furyl)acryloyl-Tyr-Lys-Arg
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
18
3-(2-furyl)acryloyl-Tyr-Lys-Arg
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
7.6
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
13.9
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
24.3
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
32
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
1.5
Acetyl-Tyr 4-nitroanilide
Escherichia coli
-
-
23
Ala-Phe-Lys-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
34
Ala-Phe-Lys-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
1
Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
2
Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
21
Arg-Arg-2-naphthylamide
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
30
Arg-Arg-2-naphthylamide
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
29
Arg-Arg-Arg
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
38
Arg-Arg-Arg
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
0.024
Benzoyl-Arg 2-naphthylamide
Escherichia coli
-
-
5496
benzoyl-Arg-4-nitroanilide
Escherichia coli
-
pH 7.10, 25C
5500
benzoyl-Arg-4-nitroanilide
Escherichia coli
-
pH 7.10, 25C
6850
benzoyl-Arg-4-nitroanilide
Escherichia coli
-
pH 8.00, 25C
6852
benzoyl-Arg-4-nitroanilide
Escherichia coli
-
pH 8.00, 25C
11290
benzoyl-Arg-4-nitroanilide
Escherichia coli
-
pH 8.03, 25C
11300
benzoyl-Arg-4-nitroanilide
Escherichia coli
-
pH 8.03, 25C
6.61
benzoyl-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
-
13.3
benzoyl-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild-type, pH not specified in the publication, temperature not specified in the publication
-
13.4
benzoyl-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
-
22.8
benzoyl-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
-
33.9
benzoyl-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
-
4848
benzoyl-Arg-beta-naphthylamide
Escherichia coli
-
pH 8.00, 25C
4850
benzoyl-Arg-beta-naphthylamide
Escherichia coli
-
pH 8.00, 25C
6830
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
Leishmania donovani
C9EF60
pH 8.0, 25C
4250
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
Leishmania donovani
C9EF60
pH 8.0, 25C
0.016
benzoyl-L-Arg-7-amido-4-methylcoumarin
Leishmania major
Q4QHU7
21C, pH not specified in the publication
0.758
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E607Q, at 37C
1.42
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609R, at 37C
1.46
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609F, at 37C
1.64
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E607Q/E609Q, at 37C
4.98
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609L, at 37C
6.84
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609A, at 37C
7.81
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609Q, at 37C
8.29
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild type enzyme, at 37C
9.36
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E610Q, at 37C
11.2
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609Q/E610Q, at 37C
21.2
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
201.7
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
4.4
Benzoyl-Lys 2-naphthylamide
Escherichia coli
-
-
5.82
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild-type, pH not specified in the publication, temperature not specified in the publication
8.72
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
11.4
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
11.8
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
14.3
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
3.62
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
-
3.91
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
-
7
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
-
8.29
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild-type, pH not specified in the publication, temperature not specified in the publication
-
9.55
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
-
120
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant E576A
240
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant E578A
420
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant D460T/D462N
540
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, wild-type, mutant E494H, D599H and D567H
600
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, opdB with polyhistidine affinity tag removed, mutant D460T, D462N and E624H
660
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant D638Q
17
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
26
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
540
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant E576A and E578A
1080
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant D460T/D462N
1320
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant D460T
1380
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant D462N
1740
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C wild-type and mutant D567H
1800
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant D599H
1860
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant D638Q
1980
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant E494H and E624H
2040
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, opdB with polyhistidine affinty tag removed
59
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
64
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
1.38
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
-
4.25
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
-
5.16
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild-type, pH not specified in the publication, temperature not specified in the publication
-
6.64
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
-
10
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
-
900
benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
1500
benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
33
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C,recombinant enzyme
48
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
0.013
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin
Leishmania major
Q4QHU7
21C, pH not specified in the publication
1.24
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E607Q, at 37C
1.58
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E607Q/E609Q, at 37C
1.77
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609F, at 37C
5.03
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609L, at 37C
5.37
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609R, at 37C
5.42
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609A, at 37C
9.53
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609Q/E610Q, at 37C
13.3
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild type enzyme, at 37C
15.2
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E610Q, at 37C
15.8
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609Q, at 37C
1.17
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
-
2.91
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
-
3.36
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild-type, pH not specified in the publication, temperature not specified in the publication
-
6.93
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
-
12.3
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
-
420
benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
420
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant E576A
540
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant E578A
1020
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant D460T/D462N
1080
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant D460T and E494H
1140
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant D462N
1200
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant D599A and D638Q
1260
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
1320
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C, mutant D567H and E624H
59
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
77
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
900
benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
1020
Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
1380
Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
1200
Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
1560
Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
1080
Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
960
Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
1080
Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
11
Gly-Arg-2-naphthylamide
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
12
Gly-Arg-2-naphthylamide
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
40
Gly-Gly-Lys-Arg
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
54
Gly-Gly-Lys-Arg
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
197
N-benzoyl-Arg ethyl ester
Escherichia coli
-
-
3.5
N-benzoyl-arginine amide
Escherichia coli
-
-
57
N-benzoyl-arginine amide
Escherichia coli
-
-
0.02
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
Leishmania major
Q4QHU7
21C, pH not specified in the publication
0.01
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin
Leishmania major
Q4QHU7
21C, pH not specified in the publication
0.011
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
Leishmania major
Q4QHU7
21C, pH not specified in the publication
43
N-benzyloxycarbonyl-Lys 4-nitrophenyl ester
Escherichia coli
-
-
55
N-Benzyloxycarbonyl-Lys benzyl ester
Escherichia coli
-
-
14.5
Nalpha-benzoyl-DL-arginine-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
37.7
Nalpha-benzoyl-DL-arginine-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
53.8
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
55.5
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
Serratia proteamaculans
-
pH 8.0, 25C
2 - 8
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
35.2
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
17
Phe-Arg-2-naphthylamide
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
18
Phe-Arg-2-naphthylamide
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
16
Phe-Arg-Arg
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
21
Phe-Arg-Arg
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
1
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.5
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
1.2
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
102
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
122
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, ecombinant mutant enzyme S563A
9
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
12
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
81
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
101
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
55
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C,recombinant enzyme
76
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
108
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
154
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
57
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
74
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
94
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
132
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
58
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
64
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
18
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
22
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
8
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
12
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
780
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
1080
Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide
Salmonella enterica
Q9L6C8
pH 8.0, 37C
60
tosyl-Arg methyl ester
Escherichia coli
-
-
55
Tosyl-Lys methyl ester
Escherichia coli
-
-
38
Val-Lys-Arg-Arg
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, recombinant enzyme
44
Val-Lys-Arg-Arg
Trypanosoma evansi
Q6QDP1
pH 8.0, 37C, native enzyme
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
30
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
19603
34
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
19603
10.8
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
12190
120
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
12190
310
benzoyl-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
202427
330
benzoyl-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
202427
4010
benzoyl-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
202427
4040
benzoyl-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
202427
4960
benzoyl-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild-type, pH not specified in the publication, temperature not specified in the publication
202427
2218000
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
Leishmania donovani
C9EF60
pH 8.0, 25C
41926
547700
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
Leishmania donovani
C9EF60
pH 8.0, 25C
41925
3.6
benzoyl-L-Arg-7-amido-4-methylcoumarin
Leishmania major
Q4QHU7
21C, pH not specified in the publication
3155
50
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609F, at 37C
3155
67
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E607Q, at 37C
3155
77
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609R, at 37C
3155
129
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E607Q/E609Q, at 37C
3155
551
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609L, at 37C
3155
647
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609Q/E610Q, at 37C
3155
677
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609A, at 37C
3155
1480
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609Q, at 37C
3155
1650
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E610Q, at 37C
3155
2480
benzoyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild type enzyme, at 37C
3155
251
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
10352
377
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
10352
1460
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
37253
2310
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
37253
21600
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild-type, pH not specified in the publication, temperature not specified in the publication
37253
33300
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
37253
69400
benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
37253
120
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
202428
190
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
202428
880
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
202428
1660
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
202428
2480
benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild-type, pH not specified in the publication, temperature not specified in the publication
202428
31
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
202429
5450
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
202429
8910
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild-type, pH not specified in the publication, temperature not specified in the publication
202429
9090
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
202429
22900
benzyloxycarbonyl-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
202429
1
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin
Leishmania major
Q4QHU7
21C, pH not specified in the publication
41923
83
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E607Q, at 37C
3568
217
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E607Q/E609Q, at 37C
3568
250
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609R, at 37C
3568
265
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609F, at 37C
3568
750
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609Q/E610Q, at 37C
3568
790
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609L, at 37C
3568
1030
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609A, at 37C
3568
1960
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E609Q, at 37C
3568
2590
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant enzyme E610Q, at 37C
3568
4960
benzyloxycarbonyl-L-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild type enzyme, at 37C
3568
66
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E172Q, pH not specified in the publication, temperature not specified in the publication
202416
340
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant R689A, pH not specified in the publication, temperature not specified in the publication
202416
2870
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
wild-type, pH not specified in the publication, temperature not specified in the publication
202416
3370
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E490Q, pH not specified in the publication, temperature not specified in the publication
202416
19300
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
Trypanosoma brucei
-
mutant E524Q, pH not specified in the publication, temperature not specified in the publication
202416
5.3
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin
Leishmania major
Q4QHU7
21C, pH not specified in the publication
41924
1.1
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin
Leishmania major
Q4QHU7
21C, pH not specified in the publication
37348
3.6
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
Leishmania major
Q4QHU7
21C, pH not specified in the publication
41413
125
Nalpha-benzoyl-DL-arginine-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
6214
161
Nalpha-benzoyl-DL-arginine-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
6214
2
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
19602
5.8
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
Serratia proteamaculans
-
pH 8.0, 25C
19602
485
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
10351
680
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
10351
0.07
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
36168
0.4
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
36168
0.5
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
Serratia proteamaculans
-
pH 8.0, 25C
19604
3
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide
Serratia proteamaculans
-
presence of 50 mM Ca2+, pH 8.0, 25C
19604
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.4
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0.188
agmatine
pH 8.0, 37C, native enzyme
0.207
agmatine
pH 8.0, 37C, recombinant enzyme
0.00000181
antipain
-
0.0000081
antipain
pH 8.0, 37C
0.001
antipain
pH 8.0, 37C, recombinant enzyme
0.002
antipain
pH 8.0, 37C, native enzyme
0.1867
benzamidine
pH 8.0, 37C
0.218
benzamidine
pH 8.0, 37C, native enzyme
0.254
benzamidine
pH 8.0, 37C, recombinant enzyme
0.05
benzoyl-Arg-4-nitroanilide
-
pH 8.10, 25C
0.027
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0.19
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.000035
bovine basic pancreatic trypsin inhibitor
-
substrate Nalpha-benzoyl-DL-Arg-4-nitroanilide, pH 8.0, 25C
-
0.00735
bovine basic pancreatic trypsin inhibitor
-
substrate Nalpha-benzoyl-DL-Arg-4-nitroanilide, presence of 50 mM Ca2+, pH 8.0, 25C
-
0.00003
Leupeptin
-
0.0004
Leupeptin
pH 8.0, 37C
0.021
Leupeptin
pH 8.0, 37C, native enzyme
0.029
Leupeptin
pH 8.0, 37C, recombinant enzyme
0.31
m-phenanthroline
-
substrate Nalpha-benzoyl-DL-Arg-4-nitroanilide, pH 8.0, 25C
0.44
m-phenanthroline
-
substrate acetyl-Leu-Leu-Arg-4-nitroanilide, pH 8.0, 25C
0.045
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
pH 8.0, 25C
0.126
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
presence of 50 mM Ca2+, pH 8.0, 25C
0.82
o-phenanthroline
-
substrate acetyl-Leu-Leu-Arg-4-nitroanilide, pH 8.0, 25C
1.59
o-phenanthroline
-
substrate Nalpha-benzoyl-DL-Arg-4-nitroanilide, pH 8.0, 25C
0.1171
p-aminobenzamidine
pH 8.0, 37C
0.0034
Pentamidine
-
0.00001
protamine
-
0.0067
suramin
-
0.0244
ZnCl2
-
substrate Nalpha-benzoyl-DL-Arg-4-nitroanilide, pH 8.0, 25C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.01
trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester
Escherichia coli
-
IC50: about 0.01 mM. Oligopeptidase B is much more sensitive than oligopeptidase B
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0001
-
mutant enzyme E607Q/E609Q, towards benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin, at 37C
0.000323
-
mutant enzyme E607Q, towards benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin, at 37C
0.011
-
mutant enzyme E609Q/E610Q, towards benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin, at 37C
0.0209
-
mutant enzyme E610Q, towards benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin, at 37C
0.0221
-
mutant enzyme E609Q, towards benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin, at 37C
0.0279
-
wild type enzyme, towards benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin, at 37C
5
substrate t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, pH 7.8, 30C
6
substrate t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin, pH 7.8, 30C; substrate t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, pH 7.8, 30C; substrate t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, pH 7.8, 30C
13
substrate t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin, pH 7.8, 30C
18
substrate benzoyl-Arg-7-amido-4-methylcoumarin, pH 7.8, 30C
42 - 60
substrate t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
53
substrate benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, pH 7.8, 30C
72.6
-
pH 8.0, temperature not specified in the publication
86
substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin, pH 7.8, 30C; substrate t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin, pH 7.8, 30C
90
-
substrate t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, pH 8.0, 30C
100
substrate t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin, pH 7.8, 30C
105
-
substrate benzoyl-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
124
-
substrate t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
126
-
substrate t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
158
substrate t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin, pH 7.8, 30C
170
-
substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
176
-
purified enzyme
220
-
substrate t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
243
-
substrate t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, pH 8.0, 30C
328
-
substrate benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
340
substrate benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, pH 7.8, 30C
370
-
substrate t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
382
-
substrate t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
408
-
substrate t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
457
substrate t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, pH 8.0, 30C
519
substrate t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, pH 8.0, 30C
570
-
substrate benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
645
substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
663
substrate t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
908
substrate benzoyl-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
1230
substrate t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
1300
substrate t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
1310
substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
1330
substrate t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, pH 8.0, 30C
1430
substrate benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
1810
substrate benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
1850
substrate t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, pH 8.0, 30C
2230
substrate t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
2380
substrate t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
2470
substrate t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
3000
substrate t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
3055
substrate t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
3130
substrate benzoyl-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
3275
substrate t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
3440
substrate benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
3500
substrate benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
3500
substrate t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
3690
substrate t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin, pH 8.0, 30C
additional information
-
-
additional information
-
the specific activity of OpdB in Escherichia coli increases under conditions where the carbon levels are limiting
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.3
-
pH optimum in the presence of NaCl
7.5 - 9
-
N-benzoyl-DL-arginine 4-nitroanilide
7.6 - 8.9
-
azocasein
8.5
hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
8.8
-
pH optimum in the absence of NaCl
additional information
-
pI: 5.2
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7
-
about 80% of maximum activity
7 - 10.5
pH 7.0: about 50% of maximal activity, pH 10.5: about 50% of maximal activity, hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
additional information
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
optimum temperature for the reactions of amide substrates slightly below
25
-
optimum temperature for the reactions of amide substrates slightly below
25
-
the abnormally low temperature optimum may be caused by the disruption of the hydrogen bond formed between the enzyme and the NH group of the scissile peptide bond
37
-
assay at
39
-
benzoyl-arginine ethyl ester
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10
-
about 60% of maximum activity
60
-
activity is reduced by 90% at 60C for 10 min
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.5
-
estimated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
enzyme is secreted
Manually annotated by BRENDA team
-
harvested at late log-phase
Manually annotated by BRENDA team
additional information
Trypanosoma evansi releases oligopeptidase B into the plasma of infected hosts
Manually annotated by BRENDA team
additional information
oligopeptidase B gene is expressed in all cycle stages
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
58000
-
E. coli, sucrose density gradient centrifugation, gel filtration
29785
78000
His-tagged enzyme, SDS-PAGE
707457
78970
predicted from opdB gene open reading frame
651697
81000
-
E. coli, gel filtration
29784
81860
-
E. coli, calculation from nucleotide sequence
29784
81860
-
amino acid sequence
649879, 650148
83490
predicted from cDNA
683954
120000
-
683194
120000
-
PAGE
732683
160000
-
gel filtration
708953
189000
-
gel filtration
732683
additional information
-
Ser532 is the reactive serine residue
29784
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 60000, SDS-PAGE
?
-
x * 60000, SDS-PAGE
?
-
x * 80000, SDS-PAGE under reducing and non-reducing conditions
?
-
x * 78000, SDS-PAGE
dimer
-
2 * 80000, SDS-PAGE
dimer
Trypanosoma cruzi Brener
-
2 * 80000, SDS-PAGE
-
homodimer
-
2 * 84000, calculated from amino acid sequence
monomer
-
1 * 80000, SDS-PAGE without mercaptoethanol
monomer
-
1 * 58000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using the hanging-drop vapour-diffusion technique in 7%(w/v) polyethylene glycol 6000, 1 M LiCl, 0.1 M bis-tris propane pH 7.5. Diffraction data to 2.7 A resolution are collected using synchrotron radiation. The crystals belong to space group P3121 or P3221, with unit-cell parameters a = b = 124.5, c = 249.9 A. A complete data set to 2.7 A is collected using synchrotron radiation
in complex with the inhibitor antipain and as mercury derivative, to 1.65 and 2.0 A resolution, respectively. OPB comprises two domains, a catalytic and a propeller domain, linked together by a hinge region. Residue E621 plays a critical role in the S1 binding pocket and, along with F603, is largely responsible for the enzyme substrate specificity in P1. In the S2 binding pocket, Y499 is important for substrate stability. Residue E623 forms an inter-domain hydrogen bond
crystal structure solved at 2.7 A resolution
using the hanging-drop vapour-diffusion technique in 7% (w/v) polyethylene glycol 6000, 1 M LiCl, 0.1 M bis-tris propane pH 7.5. Diffraction data to 2.7 A resolution are collected using synchrotron radiation. The crystals belong to space group P3121 or P3221, with unit-cell parameters a = b = 124.5, c = 249.9 A. A complete data set to 2.7 A is collected using synchrotron radiation
-
ligand-free open state and inhibitor-bound closed state crystal structures, to 2.4 and 2.85 A resolution, respectively. Peptides over 30 residues cannot fit the enzyme cavity, preventing the complete domain closure required for a key propeller Asp/Glu to fix the catalytic His and Arg in the catalytically competent conformation. This size exclusion mechanism protects larger peptides and proteins from degradation
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5 - 9.5
-
stable
29784, 29786
6
-
quick inactivation below
29787
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
-
rapid loss of activity above
717235
30
30 min, 25% loss of activity
731412
37
-
fluorescence intensity of enzyme decreases with increasing heat treatment time and the maximum fluorescence wavelength shifts slightly towards longer wavelength, 340-345 nm and 342-346 nm. The fluorescence intensity in 50% glycerol does not change significantly following heat treatment. The maximum fluorescence wavelength also shifts slightly, 337-340 nm and 339-340 nm
732874
40
-
3 h, stable
29785
40
30 min, 75% loss of activiy
731412
42
-
30 min, wild-type, 89% residual activity, mutants E172Q, E490Q, E524Q and R689A 99%, 9%, 49% and 73% residual activity, respectively
732011
42
-
enzyme retains its dimeric structure and is fully active up to 42C
732683
45
-
30 min, pH 8.0, 50% loss of activity
29784, 29786
45
-
15 min, stable below
670791
45 - 53
-
following treatment at 45C for 20 min the relative activity of wild type enzyme is 89%, the melting temperature of the wild type enzyme is at 53.3C
708953
50
-
15 min, complete inactivation
670791
50
15 min, complete loss of activity
731412
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Ca2+ decreases stability
-
Repeated freezing and thawing does not affect esterase activity
-
the wild type enzyme is not digested by trypsin at 25C
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimethyl sulfoxide
-
assay in presence of 2% dimethyl sulfoxide
Glycerol
-
the fluorescence intensity in 50% glycerol does not change significantly following heat treatment. The maximum fluorescence wavelength also shifts slightly, 337-340 nm and 339-340 nm
urea
-
the wild type enzyme is resistant to treatment with 3 M urea
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80C, 5 weeks, without or with 25 and 50 mM CaCl2, the remaining activities are 88%, 50% and 32% respectively
-
-80C, enzyme form A loses its activity after 3 months of storage, enzyme form B remains stable for more than one year
-
-80C, maximal stability in dilute solutions at protein concentrations no less than 0.3 mg/ml, pH 7.8
-
-20C, stable for several days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by affinity chromatography and separation of enzyme species A and B
-
HB101 gene cloned and expressed in JM83
-
recombinant
-
purified by using using metal-affinity chromatography
QSepharose column chromatography, ultrafiltration, Ni-NTA-agarose column chromatography, and immobilized basic pancreatic trypsin inhibitor-Sepharose column chromatography
TALON metal affinity resin column chromatography
-
Ni-NTA agarose column chromatography
-
purified by using metal-affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Escherichia coli HB101 gene cloned and expressed in Escherichia coli JM83
-
expression in Escherichia coli
-
fusion expression of oligopeptidase B with an N-terminal histidine tag using pET28a as the expression vector. Although most of the recombinant OpdB is produced as inclusion bodies, the solubility of the recombinant protease increases significantly when expression temperature shifts from 37C to 30C
-
location of the protease II gene on the physical map of the Escherichia coli chromosome
-
overexpressed in Escherichia coli
-
overexpressed in Escherichia coli JM83
-
Oligopeptidase B is overexpressed in Escherichia coli as an N-terminally hexahistidine-tagged fusion protein
expression in Escherichia coli
expression in Escherichia coli
opdB gene PCR amplified and expressed in Escherichia coli BL21(lambdaDE3)
expression in Escherichia coli
expressed in Escherichia coli BL-21(DE3) cells
expression in Escherichia coli
-
expression in Escherichia coli
expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
-
Oligopeptidase B is overexpressed in Escherichia coli as an N-terminally hexahistidine-tagged fusion protein
-
expressed in Escherichia coli
-
expression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Y452F
-
site-specific mutagenesis
D460T
site-mutated variant
D460T/D462N
site-mutated variant
D462N
site-mutated variant
D567H
site-mutated variant
D599H
site-mutated variant
D638Q
site-mutated variant
E494H
site-mutated variant
E576A
site-mutated variant
E576A/E578A
site-mutated variant
E576R/E578R
site-mutated variant
E576W/E578T
site-mutated variant
E578A
site-mutated variant
E624H
site-mutated variant
C544A
-
the enzymatic activity of the Cys-free mutant is markedly activated by the general thiol-reacting reagent dithiothreitol, oxidized glutathione, and reduced glutathione,the mutant is insensitive to the treatment of the SH-blocking reagent N-ethylmaleimide or H2O2
C256S
-
750fold reduction in inhibition rate constant for iodoacetic acid and more than 100fold reduction in inhibition rate constant for NEM compared to wild-type enzyme
C559S
mutant shows significant decrease in activation by reducing agents. This residue may thus mediate the activity enhancing effect of reducing agents
C597S
mutant shows significant decrease in activation by reducing agents. This residue may thus mediate the activity enhancing effect of reducing agents
E172Q
-
mutation in residue predicted to form interdomain salt bridges, residue is critical for catalytic activity
E490Q
-
mutation in residue predicted to form interdomain salt bridges. Mutant exhibits markedly decreased thermal stability. The boundary regions between the beta-propeller domain and catalytic domains of the E490Q mutant are unfolded with heat treatment
E524Q
-
mutation in residue predicted to form interdomain salt bridges
E607Q
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E607Q/E609Q
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E609A
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E609F
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E609L
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E609Q
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E609Q/E610Q
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
E609R
-
the mutant shows reduced kcat/Km compared to the wild type enzyme
R689A
-
mutation in residue predicted to form interdomain salt bridges
S563A
catalytically inactive OpdB variant, no activity with benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin and tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
additional information
-
mutation of Tyr452 confirmed that this residue is involved in the catalytic mechanism by stabilising the intermediate consisting of an oxoanion. Furthermore, it is shown that this oxanion binding site prevents non-productive substrate binding
E610Q
-
the mutant shows reduced kcat/Km compared to the wild type enzyme, the mutation results in a marked decrease in thermal stability, the mutant is inactivated to 63% of the control by 3 M urea
additional information
cys256 is identified as the reactive cysteine residue responsible for inactivation by N-ethylmaleimide and iodoacetic acid
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
-
involved in host cell invasion, important target for drug design
medicine
potential theraputic target in infectious disease