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EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Preferential cleavage: Leu-/-, Met-/- and Phe-/-. Hydrolyses elastin
Synonyms
elastase 2, elastase-2, ela-2, pancreatic elastase ii, bovine pancreatic elastase, neutrophil elastase 2,
more
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bovine pancreatic elastase
-
-
neutrophil elastase 2
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ELA2
-
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Elastase 2
-
-
-
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elastase-2
-
-
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Preferential cleavage: Leu-/-, Met-/- and Phe-/-. Hydrolyses elastin
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-
-
-
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hydrolysis of peptide bond
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-
-
-
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collagen fiber + H2O
?
-
-
-
-
?
elastic fiber + H2O
?
-
-
-
-
?
Elastin + H2O
Hydrolyzed elastin
Fibronectin + H2O
?
-
-
-
-
?
Gly-Ile-Asp-Val-Gln-Ile-Tyr + H2O
Gly-Ile-Asp-Val-Gln + Ile-Tyr + ?
-
sequence in phenylalanine oxidase
-
?
N-succinyl-Ala-ALa-Pro-Leu-P-nitroanilide + H2O
?
-
-
-
-
?
SMS 201-995 + H2O
?
-
-
-
?
Suc-Ala-Ala-Pro-Met-p-nitroanilide + H2O
p-nitroaniline + Suc-Ala-Ala-Pro-Met
-
-
-
?
Suc-Ala-Ala-Val-Ala-p-nitroanilide + H2O
p-nitroaniline + Suc-Ala-Ala-Val-Ala
-
-
-
?
Suc-Tyr-Leu-Val-p-nitroanilide + H2O
p-nitroaniline + Suc-Tyr-Leu-Val
-
-
-
?
additional information
?
-
(pro)-filaggrin + H2O
?
-
direct degradation
-
-
?
(pro)-filaggrin + H2O
?
-
direct degradation
-
-
?
Elastin + H2O
Hydrolyzed elastin
-
-
-
?
Elastin + H2O
Hydrolyzed elastin
-
-
-
?
Elastin + H2O
Hydrolyzed elastin
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-
-
?
Somatostatin-14 + H2O
?
-
-
-
?
Somatostatin-14 + H2O
?
-
hydrolytic enzyme required for intestinal digestion of food stuff, natural substrates are proteins, protein fragments and peptides, greater affinity for natural substrates than for synthetics, specifically involved in the regulation of biological functions of somatostatin-14 in the gastrointestinal tract
-
?
additional information
?
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-
the enzyme is formed by activation of proelastase II from mammalian pancreas by trypsin, usually only one of the pancreatic elastases (see also EC 3.4.21.36) is expressed in a given species
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-
?
additional information
?
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the enzyme is formed by activation of proelastase II from mammalian pancreas by trypsin, usually only one of the pancreatic elastases (see also EC 3.4.21.36) is expressed in a given species
-
-
?
additional information
?
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the enzyme is formed by activation of proelastase II from mammalian pancreas by trypsin, usually only one of the pancreatic elastases (see also EC 3.4.21.36) is expressed in a given species
-
-
?
additional information
?
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elastin-Congo red is no substrate
-
?
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collagen fiber + H2O
?
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-
-
-
?
elastic fiber + H2O
?
-
-
-
-
?
Fibronectin + H2O
?
-
-
-
-
?
Somatostatin-14 + H2O
?
-
hydrolytic enzyme required for intestinal digestion of food stuff, natural substrates are proteins, protein fragments and peptides, greater affinity for natural substrates than for synthetics, specifically involved in the regulation of biological functions of somatostatin-14 in the gastrointestinal tract
-
?
additional information
?
-
(pro)-filaggrin + H2O
?
-
direct degradation
-
-
?
(pro)-filaggrin + H2O
?
-
direct degradation
-
-
?
additional information
?
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the enzyme is formed by activation of proelastase II from mammalian pancreas by trypsin, usually only one of the pancreatic elastases (see also EC 3.4.21.36) is expressed in a given species
-
-
?
additional information
?
-
-
the enzyme is formed by activation of proelastase II from mammalian pancreas by trypsin, usually only one of the pancreatic elastases (see also EC 3.4.21.36) is expressed in a given species
-
-
?
additional information
?
-
-
the enzyme is formed by activation of proelastase II from mammalian pancreas by trypsin, usually only one of the pancreatic elastases (see also EC 3.4.21.36) is expressed in a given species
-
-
?
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additional information
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most metal ions have almost no effect on the activity
additional information
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increasing Ca2+ activity has no effect on elastase activity
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3,4-dichloroisocoumarin
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causes total inhibition
alpha1-Aantichymotrypsin
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-
-
Cu2+
-
1 mM, 50% inhibition
diisopropyl fluorophosphate
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causes total inhibition
Hg2+
-
1 mM, 50% inhibition
Human alpha1-proteinase inhibitor
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-
-
N-alpha-tosyl-L-lysine-chloromethyl ketone
-
most potent inhibitor
phenylmethylsulfonyl fluoride
secretory leukoprotease inhibitor
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skin-derived antileukoprotease
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Soybean trypsin inhibitor
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most potent inhibitor
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phenylmethylsulfonyl fluoride
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completely inhibited
phenylmethylsulfonyl fluoride
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1 mM, 50% inhibition
secretory leukoprotease inhibitor
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-
-
secretory leukoprotease inhibitor
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-
-
skin-derived antileukoprotease
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-
skin-derived antileukoprotease
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-
-
additional information
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TLCK and TPCK have only a small effect on the activity
-
additional information
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enzyme activity is not affected by TPCK nor by phosphoramidon
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additional information
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the enzyme needs to be proteolytically activated e.g. by trypsin, proelastase 2 is not activated by cathepsin B
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kallikrein 5
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kallikrein 5 leads to an elastolytic activity increase of 4times compared with the basal activity level of pro-elastase 2
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kallikrein 5
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kallikrein 5 leads to an elastolytic activity increase of 4times compared with the basal activity level of pro-ELA2
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Aneurysm
Assessment of the role of pancreatic proteases in human abdominal aortic aneurysms and occlusive disease.
Aortic Aneurysm, Abdominal
Assessment of the role of pancreatic proteases in human abdominal aortic aneurysms and occlusive disease.
COVID-19
Inflammation Profiling of Critically Ill Coronavirus Disease 2019 Patients.
Genetic Diseases, Inborn
Comparative molecular docking analysis of essential oil constituents as elastase inhibitors.
Infections
Effects of a high-protein diet on host resistance to Paracoccidioides brasiliensis in mice.
Infections
What is the performance of novel synovial biomarkers for detecting periprosthetic joint infection in the presence of inflammatory joint disease?
Joint Diseases
What is the performance of novel synovial biomarkers for detecting periprosthetic joint infection in the presence of inflammatory joint disease?
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Association between BMI-1 expression, acute graft-versus-host disease, and outcome following allogeneic stem cell transplantation from HLA-identical siblings in chronic myeloid leukemia.
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Expression of the leukemic prognostic marker CD7 is linked to epigenetic modifications in chronic myeloid leukemia.
Myocardial Infarction
Elastase-2, an angiotensin II-generating enzyme, contributes to increased angiotensin II in resistance arteries of mice with myocardial infarction.
Neoplasms
Inflammation Profiling of Critically Ill Coronavirus Disease 2019 Patients.
Neoplasms
Neutrophil extracellular trap formation in supragingival biofilms.
Neoplasms
The TLR4 Agonist Fibronectin Extra Domain A is Cryptic, Exposed by Elastase-2; use in a fibrin matrix cancer vaccine.
Netherton Syndrome
Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing.
Netherton Syndrome
[Elastase 2, a key player in the integrity of the epidermal barrier and in Netherton syndrome.]
Neutropenia
Heterozygous M1V variant of ELA-2 gene mutation associated with G-CSF refractory severe congenital neutropenia.
Neutropenia
Homozygous HAX1 mutations in severe congenital neutropenia patients with sporadic disease: a novel mutation in two unrelated British kindreds.
Neutropenia
Kostmann syndrome or infantile genetic agranulocytosis, part two: Understanding the underlying genetic defects in severe congenital neutropenia.
Neutropenia
Low levels of serum elastase are not associated with mutations in ELA-2 elastase encoding gene in chronic idiopathic neutropenia.
Pancreatic Diseases
Human serum alpha 1-antichymotrypsin is an inhibitor of pancreatic elastases.
Pancreatitis
Isolation and characterization of a pancreatic elastase from plasma of patients with acute pancreatitis.
Pancreatitis
Studies on immunoreactive pancreatic elastase 2 in human serum.
Pancreatitis
Studies on the ascites fluid of acute pancreatitis in man.
Pulmonary Emphysema
Comparative molecular docking analysis of essential oil constituents as elastase inhibitors.
Retinal Vein Occlusion
Higher Aqueous Levels of Resistin and Lipocalin-2 Indicated Worse Visual Improvement following anti-VEGF Therapy in Patients with Retinal Vein Occlusion.
Wiskott-Aldrich Syndrome
Homozygous HAX1 mutations in severe congenital neutropenia patients with sporadic disease: a novel mutation in two unrelated British kindreds.
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0.0083
Gly-Ile-Asp-Val-Gln-Ile-Tyr
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pH 8.0, 25°C
0.089
Somatostatin-14
-
pH 8.0, 25°C
1.567
Suc-Ala-Ala-Pro-Met-p-nitroanilide
-
pH 8.0, 25°C
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2.1
Gly-Ile-Asp-Val-Gln-Ile-Tyr
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pH 8.0, 25°C
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0.0000299
secretory leukoprotease inhibitor
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pH and temperature not specified in the publication
-
0.0000143
skin-derived antileukoprotease
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pH and temperature not specified in the publication
-
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additional information
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specific activity 118.83, activity of the enzyme is determined by its ability to degrade 125I-S-14, ng degraded/mg protein
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5.6 - 5.9
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isoelectric focusing
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bovine
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brenda
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-
brenda
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-
-
brenda
rat, male Wistar
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-
brenda
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-
brenda
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-
brenda
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-
brenda
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-
brenda
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-
brenda
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-
brenda
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-
brenda
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-
brenda
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-
brenda
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localized to keratohyalin granules
brenda
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localized to keratohyalin granules
brenda
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pancreatic juice
-
brenda
-
-
brenda
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physiological function
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elastase 2 impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing. The enzyme is a major protease driving the pathogenesis of Netherton syndrome
physiological function
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elastase 2 impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing. The enzyme is a major protease driving the pathogenesis of Netherton syndrome. Transgenic elastase 2 mice display an ichthyotic phenotype
physiological function
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expression of ELA2 is associated with histone acetylation in leukemic cell lines. DNA methylation and transcript levels of ELA2 in chronic myeloid leukemia are not correlated
physiological function
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the enzyme's biological functions include involvement in migration of neutrophils by means of focal proteolysis, killing of microorganisms, and degradation/activation of various proteins including cytokines, chemokines and ECM proteins
physiological function
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the enzyme plays a role in the maintenance of basal hemodynamics, contributing to cardiovascular homeostasis. The enzyme acts on the sympathovagal balance of the heart
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CEL2A_BOVIN
269
0
28857
Swiss-Prot
Secretory Pathway (Reliability: 2 )
CEL2A_HUMAN
269
0
28888
Swiss-Prot
Secretory Pathway (Reliability: 2 )
CEL2A_MOUSE
271
0
28914
Swiss-Prot
Secretory Pathway (Reliability: 1 )
CEL2A_PIG
269
0
28699
Swiss-Prot
Secretory Pathway (Reliability: 2 )
CEL2A_RAT
271
0
28885
Swiss-Prot
Secretory Pathway (Reliability: 1 )
CEL2B_HUMAN
269
0
28810
Swiss-Prot
Secretory Pathway (Reliability: 1 )
B7PC99_IXOSC
205
0
22287
TrEMBL
Mitochondrion (Reliability: 4 )
B7QND4_IXOSC
123
0
13381
TrEMBL
other Location (Reliability: 2 )
B7QBW5_IXOSC
314
0
34768
TrEMBL
other Location (Reliability: 3 )
A0A8B6D915_MYTGA
314
1
35882
TrEMBL
Secretory Pathway (Reliability: 1 )
A0A8M1NJM4_DANRE
266
0
28800
TrEMBL
Secretory Pathway (Reliability: 2 )
A0A8B6DFE3_MYTGA
281
0
30175
TrEMBL
Secretory Pathway (Reliability: 2 )
A0A8B6BM27_MYTGA
262
0
27862
TrEMBL
Secretory Pathway (Reliability: 3 )
E3TE32_ICTPU
267
1
28946
TrEMBL
Secretory Pathway (Reliability: 1 )
B7QL11_IXOSC
212
0
22580
TrEMBL
Secretory Pathway (Reliability: 4 )
A0A8B6BMW1_MYTGA
269
0
28407
TrEMBL
Secretory Pathway (Reliability: 2 )
B8K480_9VIBR
361
1
38978
TrEMBL
-
A0A8B6G776_MYTGA
273
0
28673
TrEMBL
Secretory Pathway (Reliability: 2 )
A0A8B6D927_MYTGA
306
0
34958
TrEMBL
Secretory Pathway (Reliability: 2 )
Q5E0V3_ALIF1
Aliivibrio fischeri (strain ATCC 700601 / ES114)
319
1
33815
TrEMBL
-
A0A0B8NN19_9NOCA
256
1
26425
TrEMBL
-
B7QNU3_IXOSC
280
0
30524
TrEMBL
Secretory Pathway (Reliability: 1 )
C4WMV3_9HYPH
553
0
62177
TrEMBL
-
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27260
-
predicted from cDNA clone
additional information
-
primary structure determined by sequence analysis of cloned mRNA
29000
-
SDS-PAGE
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monomer
-
1 * 29000, SDS-PAGE
monomer
-
1 * 29000, SDS-PAGE
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proteolytic modification
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the enzyme needs to be proteolytically activated e.g. by trypsin, proelastase 2 is not activated by cathepsin B
additional information
-
no carbohydrate moiety detected in the structure
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40 - 50
-
incubation of purified enzyme for 1 h causes a slight loss of activity, less than 10%
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proelastase 2 from pancreatic juice by anion and cation exchange chromatography
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-
-
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serum elastase 2 concentration was higher in patients affected by photoaging if compared with healthy controls compared with subjects receiving placebo 2 weeks after the end of treatment
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serum neutrophil elastase 2 concentration is significantly decreased in patients treated with the dietary supplement
-
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Fletcher, T.S.; Shen, W.F.; Largman, C.
Primary structure of human pancreatic elastase 2 determined by sequence analysis of the cloned mRNA
Biochemistry
26
7256-7261
1987
Homo sapiens
brenda
Shirasu, Y.; Yoshida, H.; Matsuki, S.; Takemura, K.; Ikeda, N.; Shimada, Y.; Ozawa, T.; Mikayama, T.; Iijima, H.; Ishida, A.; Sato, Y.; Tamai, Y.; Tanaka, J.; Ikenaga, H.
Molecular cloning and expression in Escherichia coli of a cDNA encoding human pancreatic elastase 2
J. Biochem.
102
1555-1563
1987
Homo sapiens
brenda
Davril, M.; Laine, A.; Hayem, A.
Studies on the interactions of human pancreatic elastase 2 with human alpha 1-proteinase inhibitor and alpha 1-antichymotrypsin
Biochem. J.
245
699-704
1987
Homo sapiens
brenda
Szilagyi, C.M.; Sarfati, P.; Pradayrol, L.; Morisset, J.
Purification, characterization and substrate specificity of rat pancreatic elastase II
Biochim. Biophys. Acta
1251
55-65
1995
Rattus norvegicus
brenda
Azuma, K.; Banshou, Y.; Suzuki, H.
Bovine pancreatic elastase II cleaves Gln-Ile bond
J. Protein Chem.
20
577-584
2001
Bos taurus
brenda
Lindkvist, B.; Fajardo, I.; Pejler, G.; Borgstrom, A.
Cathepsin B activates human trypsinogen 1 but not proelastase 2 or procarboxypeptidase B
Pancreatology
6
224-231
2006
Homo sapiens
brenda
Bonnart, C.; Deraison, C.; Lacroix, M.; Uchida, Y.; Besson, C.; Robin, A.; Briot, A.; Gonthier, M.; Lamant, L.; Dubus, P.; Monsarrat, B.; Hovnanian, A.
Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing
J. Clin. Invest.
120
871-882
2010
Homo sapiens, Mus musculus
brenda
Rogers, S.L.; Zhao, Y.; Jiang, X.; Eaves, C.J.; Mager, D.L.; Rouhi, A.
Expression of the leukemic prognostic marker CD7 is linked to epigenetic modifications in chronic myeloid leukemia
Mol. Cancer
9
41
2010
Homo sapiens
brenda
Di Cerbo, A.; Laurino, C.; Palmieri, B.; Iannitti, T.
A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins
J. Photochem. Photobiol. B
144C
94-103
2015
Homo sapiens
brenda
Julier, Z.; Martino, M.M.; de Titta, A.; Jeanbart, L.; Hubbell, J.A.
The TLR4 agonist fibronectin extra domain a is cryptic, exposed by elastase-2, use in a fibrin matrix cancer vaccine
Sci. Rep.
5
8569
2015
Homo sapiens
brenda
Becari, C.; Durand, M.T.; Guimaraes, A.O.; Lataro, R.M.; Prado, C.M.; de Oliveira, M.; Candido, S.C.; Pais, P.; Ribeiro, M.S.; Bader, M.; Pesquero, J.B.; Salgado, M.C.; Salgado, H.C.
Elastase-2, a tissue alternative pathway for angiotensin II generation, plays a role in circulatory sympathovagal balance in mice
Front. Physiol.
8
170
2017
Mus musculus
brenda
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