Information on EC 3.4.21.67 - Endopeptidase So

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.4.21.67
-
RECOMMENDED NAME
GeneOntology No.
Endopeptidase So
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of proteins, but not Bz-Tyr-OEt, Ac-Phe-beta-naphthylester, or Bz-Arg-Oet
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
81611-83-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
secreted soluble form of isozyme-1, SK-1, recombinant protein
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Abz-DIYISRRLL-GTFT-Tyx-A + H2O
Abz-DIYISRRLL + GTFT-Tyx-A
show the reaction diagram
-
-
-
-
?
Ac-Ala4 + H2O
?
show the reaction diagram
-
-
-
-
?
Acid-soluble peptides + H2O
?
show the reaction diagram
-
-
-
-
-
Ada protein + H2O
19000 MW polypeptide + 18000 MW fragment
show the reaction diagram
-
a DNA repair enzyme and a transcriptional activator of the expression of its own ada gene, generation of a C-terminal 19000 MW polypeptide and further processing mainly to an 18000 MW fragment
-
-
Ada protein + H2O
?
show the reaction diagram
-
involved in endogenous cleavage of Ada protein (a DNA repair enzyme and a transcriptional activator of the expression of its own ada gene), which may play a role in down-regulating the adaptive response to alkylating agents
-
-
-
casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
hydrolyzed casein
show the reaction diagram
-
-
-
-
-
Denatured bovine serum albumin + H2O
Hydrolyzed denatured bovine serum albumin
show the reaction diagram
-
-
-
-
denatured bovine serum albumine + H2O
?
show the reaction diagram
-
-
-
-
?
Globin + H2O
?
show the reaction diagram
-
-
-
-
?
Globin + H2O
Hydrolyzed globin
show the reaction diagram
-
-
-
-
-
Glucagon + H2O
?
show the reaction diagram
-
-
-
-
?
Glucagon + H2O
Hydrolyzed glucagon
show the reaction diagram
-
-
-
-
Methyl-alpha-casein + H2O
Hydrolyzed methyl-alpha-casein
show the reaction diagram
-
-
-
-
Methylhemoglobin + H2O
Hydrolyzed methylhemoglobin
show the reaction diagram
-
-
-
-
Oxidatively damaged glutamine synthetase + H2O
?
show the reaction diagram
-
to acid-soluble peptides, degradation is 5-10times faster than with the native glutamine synthetase
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Ada protein + H2O
?
show the reaction diagram
-
involved in endogenous cleavage of Ada protein (a DNA repair enzyme and a transcriptional activator of the expression of its own ada gene), which may play a role in down-regulating the adaptive response to alkylating agents
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
stimulates degradation of oxidatively damaged glutamine synthetase, even so casein hydrolysis is not affected
Mn2+
-
stimulates degradation of oxidatively damaged glutamine synthetase, even so casein hydrolysis is not affected
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
1,7-phenanthroline
-
-
4-(2-aminoethyl benzene)sulfonyl fluoride
-
i.e. AEBSF, competitive
Ac-VFRSLK-4-(2-aminoethyl benzene)sulfonyl fluoride
-
-
Pentanamide
-
-
Tos-Phe-CH2Cl
-
-
tosylphenylalanine chloromethyl ketone
-
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0014
casein
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
231
4-(2-aminoethyl benzene)sulfonyl fluoride
-
substrate Abz-DIYISRRLL-GTFT-Tyx-A, 37°C, pH 7.4
57.7
Ac-VFRSLK-4-(2-aminoethyl benzene)sulfonyl fluoride
-
substrate Abz-DIYISRRLL-GTFT-Tyx-A, 37°C, pH 7.4
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
-
-
additional information
-
pI: 6.4
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9.5
-
nearly inactive at pH-values below pH 5.0 and above 9.5
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70000
-
2 * 70000, SDS-PAGE
77000
-
2 * 77000, SDS-PAGE
140000
-
E. coli, gel filtration
150000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
10 min, stable
55
-
10 min, complete loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Divalent cations, Mg2+, 5 mM, Ca2+, 5 mM, Mn2+, 5 mM, Co2+, 0.1 mM, stabilizes the enzyme at elevated temperatures
-
Repeated freezing and thawing has no effect
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-30°C, 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2, 20% v/v glycerol, stable for at least 1 year
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE