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Information on EC 3.4.21.48 - cerevisin

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.48 cerevisin
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UNIPROT: P25580 not found.
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Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt Ac-Tyr-OEt. Does not hydrolyse peptide amides
Synonyms
proteinase yscb, yeast proteinase b, cerevisin, yeast proteinase yscb, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
baker's yeast proteinase
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baker's yeast proteinase B
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brewer's yeast proteinase
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Proteinase YSCB
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proteinase, yeast B
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yeast proteinase B
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yeast proteinase yscB
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CAS REGISTRY NUMBER
COMMENTARY hide
37288-81-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
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Pbn1p shown as an essential chaperone-like protein in the endoplasmic reticulum of yeast, essential function of the Pbn1 protein for protein processing in the membrane of the endoplasmic reticulum described, requirement for proper folding and stability of a subset of proteins in the endoplasmic reticulum indicated
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?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
processing activities of transient proteins of the endoplasmic reticulum indicated, depletion of Pbn1p abrogates processing of precursor proteins of the endoplasmic reticulum but not global exit from the endoplasmic reticulum, significant increase in the unfolded protein response pathway observed indicating defects in protein folding in the endoplasmic reticulum
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Pbn1p, encoded by the PBN1 gene; BJ 5410
SwissProt
Manually annotated by BRENDA team
Pbn1p, encoded by the PBN1 gene; BJ 5410
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
membrane, type I glycoprotein
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PBN1_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
416
0
47923
Swiss-Prot
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
generation of a yeast strain with PBN1 under control of the GAL promoter and of strains depleted of Pbn1p, transformation of Saccaromyces BJ 5410 with the PBN1 gene to generate the pGAL-HA-PBN1 strain BJ 10242, properties of strain BJ 10242 by processing activities of transient proteins of the endoplasmic reticulum indicated
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Subramanian, S.; Woolford, C.A.; Drill, E.; Lu, M.; Jones, E.W.
Pbn1p: an essential endoplasmic reticulum membrane protein required for protein processing in the endoplasmic reticulum of budding yeast
Proc. Natl. Acad. Sci. USA
103
939-944
2006
Saccharomyces cerevisiae (P25580), Saccharomyces cerevisiae BJ 5410 (P25580)
Manually annotated by BRENDA team