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Information on EC 3.4.21.43 - classical-complement-pathway C3/C5 convertase

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Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Selective cleavage of Arg-/-Ser bond in complement component C3 alpha-chain to form C3a and C3b, and Arg-/- bond in complement component C5 alpha-chain to form C5a and C5b
Synonyms
c3bbbp, c4b2a, classical pathway c3 convertase, classical c3 convertase, c4b,2a, classical pathway c5 convertase, complement c3 convertase, c4bc2a, cp c3 convertase, soluble c3 convertase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C3 convertase
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C3/C5 convertase
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C4
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C423
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C4b,2a
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C4b,2a,3b
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C5 convertase
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complement C3 convertase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY hide
56626-15-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CO5_HUMAN
1676
0
188305
Swiss-Prot
other Location (Reliability: 2)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structures of the C3b homologue cobra venom factor CVF in complex with C5, and in complex with C5 and the inhibitor SSL7, both at 4.3 A resolution. The structures reveal a parallel two-point attachment between C5 and CVF, where the presence of SSL7 only slightly affects the C5-CVF interface, explaining the IgA dependence for SSL7-mediated inhibition of C5 cleavage. CVF functions as a relatively rigid binding scaffold inducing a conformational change in C5, which positions its cleavage site in proximity to the serine protease Bb
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Laursen, N.; Andersen, K.; Braren, I.; Spillner, E.; Sottrup-Jensen, L.; Andersen, G.
Substrate recognition by complement convertases revealed in the C5-cobra venom factor complex
EMBO J.
30
606-616
2011
Homo sapiens (P01031)
Manually annotated by BRENDA team