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Information on EC 3.4.21.35 - tissue kallikrein and Organism(s) Homo sapiens and UniProt Accession Q9UBX7

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.35 tissue kallikrein
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Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
UNIPROT: Q9UBX7 not found.
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Preferential cleavage of Arg-/- bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-/- or Leu-/-. The rat enzyme is unusual in liberating bradykinin directly from autologous kininogens by cleavage at two Arg-/- bonds
Synonyms
prostate specific antigen, tissue kallikrein, glandular kallikrein, renal kallikrein, trypsin-like serine protease, prokallikrein, klk15, salivary kallikrein, glandular kallikrein 2, urinary kallidinogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tissue kallikrein 11
-
bradykininogenase
-
-
-
-
callicrein
-
-
-
-
depot-Padutin
-
-
-
-
dilminal D
-
-
-
-
EMSP1
-
-
glandular kallikrein
glandular kallikrein 2
-
-
glandular kallikrein-2
-
-
glumorin
-
-
-
-
hK10
-
-
hK11
-
-
hK13
-
-
hK14
-
-
HSCTE
-
-
Kailikang
-
commercial preparation
kallidinogenase
kallikrein
kallikrein 1
kallikrein-related peptidase
-
-
kallikrein-related peptidase 10
-
-
kallikrein-related peptidase 2
-
-
kallikrein-related peptidase 7
-
-
kidney/pancreas/salivary gland kallikrein
-
-
-
-
kininogenase
-
-
-
-
kininogenin
-
-
-
-
KLK-L1 protein
-
-
KLK-L2 protein
-
-
KLK-L3
-
hK9
KLK-S3
-
-
-
-
KLK10
-
-
KLK2
-
isoform
KLK3
-
isoform
KLK7
-
-
KLK9
-
-
neurosin
-
-
onokrein P.
-
-
-
-
P1 kallikrein
-
-
-
-
padreatin
-
-
-
-
padutin
-
-
-
-
pancreatic kallikrein
-
-
-
-
pancreatic/renal kallikrein
-
PPK
-
-
-
-
prokallikrein
zymogen
prostase
-
-
prostase/KLK-L1/ARM1/PRSS17
-
hK4
prostate specific antigen
-
-
protease M
-
-
PS kallikrein
-
-
-
-
PSA/KLK3
-
-
renal kallikrein
-
renal tissue kallikrein
-
-
-
-
RSGK-50
-
-
-
-
RUK
-
-
-
-
S01.160
-
-
-
-
S1 kallikrein
-
-
-
-
S2 kallikrein
-
-
-
-
S3 kallikrein
-
-
-
-
salivary kallikrein
-
-
-
-
SEV
-
-
-
-
submandibular enzymatic vasoconstrictor
-
-
-
-
T-kininogenase
-
-
-
-
tissue kallikrein
tissue kallikrein 1
-
-
tissue kallikrein 15
-
tissue kallikrein 4
-
-
tissue kallikrein 9
-
-
trypsin-like serine protease
-
TLSP/hippostasin/hK11
UKLK
-
-
urinary kallidinogenase
urinary kallikrein
urinary tissue kallikrein
-
-
urokallikrein
-
-
-
-
zyme
-
-
additional information
-
KLK1, KLK4, and KLK11 are serine proteases with trypsin-like activity, while KLK9 has chymotrypsin-like activity
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage of Arg-/- bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-/- or Leu-/-. The rat enzyme is unusual in liberating bradykinin directly from autologous kininogens by cleavage at two Arg-/- bonds
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
389069-73-2
-
9001-01-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
Phe-Ser-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
Pro-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
Val-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
2-aminobenzoyl-MISLMKRPPGFSPFRSS(PO3H2)RI-NH2 + H2O
2-aminobenzoyl-MISLM + KRPPGFSPFRSS(PO3H2)RI-NH2
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-MISLMKRPPGFSPFRSSRI-NH2 + H2O
2-aminobenzoyl-MISLM + KRPPGFSPFRSSRI-NH2
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-TSVIRRVQ-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-ALFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-FLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-FRSSR-Q-EDDnp + H2O
Abz-FR + SSR-Q-EDDnp
show the reaction diagram
-
-
-
?
Abz-GFSPFRSLRI-Q-EDDnp + H2O
Abz-GFSPFR + SLRI-Q-EDDnp
show the reaction diagram
-
-
-
?
Abz-GFSPFRSSRI-Q-EDDnp + H2O
Abz-GFSPFR + SSRI-Q-EDDnp
show the reaction diagram
-
-
-
?
Abz-GLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-HLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-ILFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KAFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KFFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFFSK-EDDnp + H2O
Abz-KLF + FSK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFRSK-EDDnp + H2O
Abz-KLF + RSK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSAKQ-EDDnp + H2O
Abz-KLF + SAKQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSGKQ-EDDnp + H2O
Abz-KLF + SGKQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSIKQ-EDDnp + H2O
Abz-KLF + SIKQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSLKQ-EDDnp + H2O
Abz-KLF + SLKQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSNKQ-EDDnp + H2O
Abz-KLF + SNKQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSRKQ-EDDnp + H2O
Abz-KLF + SRKQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSDQ-EDDnp + H2O
Abz-KLF + SSDQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSEQ-EDDnp + H2O
Abz-KLF + SSEQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSGQ-EDDnp + H2O
Abz-KLF + SSGQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSIQ-EDDnp + H2O
Abz-KLF + SSIQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSK-EDDnp + H2O
Abz-KLF + SSK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSKQ-EDDnp + H2O
Abz-KLF + SSKQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSLQ-EDDnp + H2O
Abz-KLF + SSLQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSNQ-EDDnp + H2O
Abz-KLF + SSNQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSQQ-EDDnp + H2O
Abz-KLF + SSQQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSRQ-EDDnp + H2O
Abz-KLF + SSRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSSQ-EDDnp + H2O
Abz-KLF + SSSQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSVQ-EDDnp + H2O
Abz-KLF + SSVQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSVKQ-EDDnp + H2O
Abz-KLF + SVKQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFTSK-EDDnp + H2O
Abz-KLF + TSK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFWSK-EDDnp + H2O
Abz-KLF + WSK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLRSSQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLYSSK-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KPFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KVFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KYFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-LLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-MISLMKLP-Q-EDDnp + H2O
Abz-MISLM + KLP-Q-EDDnp
show the reaction diagram
-
-
-
?
Abz-MISLMKRP-Q-EDDnp + H2O
Abz-MISLM + KRP-Q-EDDnp
show the reaction diagram
-
-
-
?
Abz-MISLMKRPPGFSPFRSSRI-NH2 + H2O
Abz-MISLM + KRPPGFSPF + RSSRI-NH2
show the reaction diagram
-
-
-
-
?
Abz-NLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-NLYRVE-Q-EDDnp + H2O
Abz-NLY + RVE-Q-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-QLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-RLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-VLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
acetyl-Phe-Arg ethyl ester + H2O
acetyl-Phe-Arg + ethanol
show the reaction diagram
-
-
-
-
?
acetyl-Phe-Arg methyl ester + H2O
acetyl-Phe-Arg + methanol
show the reaction diagram
-
-
-
-
?
Ala-Ala-Arg-7-amido-4-methylcoumarin + H2O
Ala-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
Ala-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
angiotensinogen + H2O
angiotensin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Tyr-4-nitrophenyl ester + H2O
benzyloxycarbonyl-Tyr + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
citrated bovine plasma + H2O
?
show the reaction diagram
-
-
-
-
?
citrated dog plasma + H2O
?
show the reaction diagram
-
-
-
-
?
D-Ile-Pro-Arg-4-nitroanilide + H2O
D-Ile-Pro-Arg + 4-nitroaniline
show the reaction diagram
-
weak activity
-
-
?
D-Pro-Phe-Arg-4-methylcoumarin-7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
D-Pro-Phe-Arg-4-nitroanilide + H2O
D-Pro-Phe-Arg + 4-nitroaniline
show the reaction diagram
-
weak activity
-
-
?
D-Pro-Phe-Phe-4-methylcoumarin-7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
D-Pro-Phe-Phe-7-amido4-methylcoumarin + H2O
D-Pro-Phe-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
D-Val-Leu-Arg-4-nitroanilide + H2O
D-Val-Leu-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
D-Val-Leu-Arg-7-amido-4-trifluoromethylcoumarin + H2O
D-Val-Leu-Arg + 7-amino-4-trifluoromethylcoumarin
show the reaction diagram
-
-
-
-
?
D-Val-Leu-Arg-p-nitroanilide + H2O
D-Val-Leu-Arg + p-nitroaniline
show the reaction diagram
D-Val-Leu-Lys-4-nitroanilide + H2O
D-Val-Leu-Lys + 4-nitroaniline
show the reaction diagram
-
weak activity
-
-
?
DL-Val-Leu-Arg-p-nitroanilide + H2O
DL-Val-Leu-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Fibronectin + H2O
?
show the reaction diagram
-
-
-
-
?
H-D-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
H-D-Pro-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
i.e. S-2302
-
-
?
human high molecular weight kininogen + H2O
?
show the reaction diagram
-
-
-
-
?
insulin-like growth factor binding protein + H2O
?
show the reaction diagram
-
-
-
-
?
insulin-like growth factor binding proteins
?
show the reaction diagram
insulin-like growth factor binding proteins
insulin-like growth factor binding protein fragments
show the reaction diagram
kininogen + H2O
bradykinin + ?
show the reaction diagram
-
-
-
-
?
kininogen + H2O
bradykinin + kinin
show the reaction diagram
kininogen + H2O
bradykinin + kinin heavy chain + kinin light chain
show the reaction diagram
-
important role in bradykinin release in seminal fluid
-
?
kininogen + H2O
kallidin + kinin heavy chain + kinin light chain
show the reaction diagram
kininogen + H2O
kinin + ?
show the reaction diagram
kininogen + H2O
kinin + bradykinin
show the reaction diagram
-
-
-
-
?
Leu-Leu-Arg-7-amido-4-methylcoumarin + H2O
Leu-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
low density lipoprotein + H2O
?
show the reaction diagram
-
-
-
?
low molecular weight kininogen + H2O
kallidin + kinin
show the reaction diagram
-
-
-
-
?
low-molecular-weight kininogen + H2O
GMISLM + KRPPGFSPFR + SSRIG
show the reaction diagram
-
KRPPGFSPFR is kallidin
-
?
low-molecular-weight kininogen + H2O
kallidin + kinin
show the reaction diagram
N-alpha-acetyl-Phe-Arg ethyl ester + H2O
N-alpha-acetyl-Phe-Arg + ethanol
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg methyl ester + H2O
N-alpha-benzoyl-L-Arg + methanol
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Lys methyl ester + H2O
N-alpha-benzoyl-L-Lys + methanol
show the reaction diagram
-
-
-
-
?
N-methoxysuccinyl-Arg-Pro-Tyr-4-nitroanilide + H2O
N-methoxysuccinyl-Arg-Pro-Tyr + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AIKFFIRE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + IRE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFQRE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + QRE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFSAE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + SAE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFSKE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + SKE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFSPE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + SPE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFSRE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + SRE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFSRQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + SRQ-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
substrate sequence is derived from the kallistatin reactive centre loop sequence
-
?
o-aminobenzoyl-AIKFFSSE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + SSE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFTRE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + TRE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-FRAPR-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-FRLVR-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-FRSSR-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-FR + SSR-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
sequence spanning the scissile Arg-Ser bond in human kininogen
-
?
o-aminobenzoyl-FRSVQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-FR + SVQ-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
sequence spanning the scissile Arg-Ser bond in bovine kininogen
-
?
o-aminobenzoyl-GFSPF-4-(aminocyclohexyl)alanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-GFSPF-4-(aminomethyl)-N-isopropylphenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-GFSPF-4-(aminomethyl)phenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-GFSPF-4-piperidinylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-GFSPF-Nim-(dimethyl)histidine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-GFSPFRAPRVQ-ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-GFSPFR + APRVQ-ethylenediamine 2,4-dinitrophenol + o-aminobenzoyl-GFSPF + RAPRVQ-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
-
preferentially hydrolyzed at Phe-Arg bond compared with Phe-Arg
-
-
?
o-aminobenzoyl-GFSPFRSSRIGEIKEEQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-GFSPFR + SSRIGEIKEEQ-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
sequence spanning the scissile Arg-Ser bond in human kininogen
-
?
o-aminobenzoyl-GFSPFRSSRQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-GFSPFR + SSRQ-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
sequence spanning the scissile Arg-Ser bond in human kininogen
-
?
o-aminobenzoyl-GFSPFRSVQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-GFSPFR + SVQ-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
sequence spanning the scissile Arg-Ser bond in bovine kininogen
-
?
o-aminobenzoyl-GFSPFRSVQVMKYEGSQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-GFSPFR + SVQVMKYEGSQ-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
sequence spanning the scissile Arg-Ser bond in bovine kininogen
-
?
o-aminobenzoyl-GFSPFRSVTVQ-ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-GFSPFR + SVTVQ-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-LMKRP-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-LM + KRP-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
o-aminobenzoyl-MTEMARRPPGFSPFRSVTVQ-NH2 + H2O
o-aminobenzoyl-MTEMAR + RPPGFSPFR + SVTVQ-NH2
show the reaction diagram
-
-
RPPGFSPFR is bradykinin
-
?
o-aminobenzoyl-MTEMARRPQ-ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-MTEMAR + RPQ-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-MTSVIRRPPGFSPFRAPRV-NH2 + H2O
des[Arg9] bradykinin + ?
show the reaction diagram
-
preferential hydrolysis of Phe-Arg compared with Arg-Ala bond, preferential release of des[Arg9] bradykinin
-
-
?
o-aminobenzoyl-MTSVIRRPQ-ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-MTSVIR + RPQ-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
-
slow hydrolysis
-
-
?
o-aminobenzoyl-Phe-4-(aminomethyl)phenylalanine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-Phe-4-(aminomethyl)phenylalanine-Ser-Arg + Gln-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-Phe-Arg-Arg--ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-Phe-Arg + Arg-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
-
slow hydrolysis
-
-
?
o-aminobenzoyl-Phe-Arg-Lys-GFSPFRSVTVQ-ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-Phe-Arg + Lys-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
-
slow hydrolysis
-
-
?
o-aminobenzoyl-Phe-Nim-(dimethyl)histidine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-Phe-Nim-(dimethyl)histidine-Ser-Arg + Gln-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-QPLGMISLMKRP-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-QPLGMISLM + KRP-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
sequence spanning the scissile Met-Lys bond in human kininogen
-
?
o-aminobenzoyl-QPLGQTSLMKRP-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-QPLGQTSLM + KRP-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
sequence spanning the scissile Met-Lys bond in bovine kininogen
-
?
o-aminobenzoyl-SVIRRVQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
-
-
-
?
polypeptide + H2O
peptides
show the reaction diagram
precursor of atrial natriuretic factor + H2O
natriuretic factor
show the reaction diagram
-
-
-
?
pro-collagenase + H2O
collagease
show the reaction diagram
-
-
-
?
pro-insulin + H2O
insulin + insulin signal peptide
show the reaction diagram
-
-
-
?
Pro-Phe-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
Pro-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Pro-Phe-Arg-Arg-7-amido-4-methylcoumarin + H2O
Pro-Phe-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
pro-renin + H2O
renin
show the reaction diagram
-
-
-
?
pro-urokinase + H2O
urokinase
show the reaction diagram
-
-
-
-
?
prolactin + H2O
lactin + ?
show the reaction diagram
-
three cleavage sites between Arg174-Arg175, Lys185-Phe186 and Arg188-Cys189
-
-
?
protein C inhibitor + H2O
?
show the reaction diagram
-
-
-
-
?
QAR-7-amido-4-methylcoumarin + H2O
QAR + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
semenogelin I + H2O
KGISSQY + SNTE
show the reaction diagram
-
-
-
?
semenogelin I + H2O
semenogelin I fragments
show the reaction diagram
-
-
-
-
?
semenogelin II + H2O
semenogelin II fragments
show the reaction diagram
-
-
-
-
?
somatostatin + H2O
?
show the reaction diagram
-
-
-
-
?
Substance P + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-Val-Pro-Phe-thiobenzyl ester + H2O
succinyl-Val-Pro-Phe + phenylmethanethiol
show the reaction diagram
-
-
-
-
?
Tosyl-Arg methyl ester + H2O
Tosyl-Arg + methanol
show the reaction diagram
-
-
-
-
?
Val-Leu-Arg-4-nitroanilide + H2O
Val-Leu-Arg + 4-nitroaniline
show the reaction diagram
-
tracheobronchial epithelial cells grown at the air/liquid interface
-
-
?
Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
Val-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Val-Pro-Arg-Arg-7-amido-4-methylcoumarin + H2O
Val-Pro-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
vascular endothelial growth factor-165 + H2O
?
show the reaction diagram
-
-
-
-
?
Vasoactive intestinal peptide + H2O
?
show the reaction diagram
-
-
-
?
VPR-7-amido-4-methylcoumarin + H2O
VPR + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Z-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
zymogen of prostate-specific antigen + H2O
prostate-specific antigen + APLILSR
show the reaction diagram
-
-
-
-
?
zymogen of urokinase-type plasminogen activator + H2O
urokinase-type plasminogen activator
show the reaction diagram
-
activation of inactive zymogen to mature active enzyme
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
insulin-like growth factor binding proteins
?
show the reaction diagram
kininogen + H2O
bradykinin + ?
show the reaction diagram
-
-
-
-
?
kininogen + H2O
bradykinin + kinin heavy chain + kinin light chain
show the reaction diagram
-
important role in bradykinin release in seminal fluid
-
?
kininogen + H2O
kallidin + kinin heavy chain + kinin light chain
show the reaction diagram
kininogen + H2O
kinin + ?
show the reaction diagram
-
-
-
-
?
kininogen + H2O
kinin + bradykinin
show the reaction diagram
-
-
-
-
?
low-molecular-weight kininogen + H2O
GMISLM + KRPPGFSPFR + SSRIG
show the reaction diagram
-
KRPPGFSPFR is kallidin
-
?
low-molecular-weight kininogen + H2O
kallidin + kinin
show the reaction diagram
-
-
-
-
?
polypeptide + H2O
peptides
show the reaction diagram
prolactin + H2O
lactin + ?
show the reaction diagram
-
three cleavage sites between Arg174-Arg175, Lys185-Phe186 and Arg188-Cys189
-
-
?
semenogelin I + H2O
KGISSQY + SNTE
show the reaction diagram
-
-
-
?
vascular endothelial growth factor-165 + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
Ca2+ enhances the enzyme activity at concentrations of 2.5 and 5 mM
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(4-amidino-phenyl)-methane-sulfonyl fluoride
2.5 mM, complete inhibition
Aprotinin
up to 40% inhibition
hK11-specific monoclonal antibody
up to 40% inhibition
-
(tris[2-carboxyethyl] phosphine) and dihydro-lipoic acid
-
13% residual activity at 3 mM
4-(2-aminoethyl)benzenesulfonyl fluoride
-
is an active-site-directed covalent inhibitor
Abz-KDFSSKQ-EDDnp
-
-
Abz-KEFSSKQ-EDDnp
-
-
Abz-KGFSSKQ-EDDnp
-
-
Abz-KHFSSKQ-EDDnp
-
-
Abz-KIFSSKQ-EDDnp
-
-
Abz-KKFSSKQ-EDDnp
-
-
Abz-KLASSK-EDDnp
-
-
Abz-KLDSSK-EDDnp
-
-
Abz-KLESSK-EDDnp
-
-
Abz-KLFESK-EDDnp
-
-
Abz-KLFHSK-EDDnp
-
-
Abz-KLFISK-EDDnp
-
-
Abz-KLFKSK-EDDnp
-
-
Abz-KLFLSK-EDDnp
-
-
Abz-KLFNSK-EDDnp
-
-
Abz-KLFPSK-EDDnp
-
-
Abz-KLFSEKQ-EDDnp
-
-
Abz-KLFSFKQ-EDDnp
-
-
Abz-KLFSHKQ-EDDnp
-
-
Abz-KLFSPKQ-EDDnp
-
-
Abz-KLFSQKQ-EDDnp
-
-
Abz-KLFVSK-EDDnp
-
-
Abz-KLGSSK-EDDnp
-
-
Abz-KLHSSK-EDDnp
-
-
Abz-KLISSK-EDDnp
-
-
Abz-KLKSSK-EDDnp
-
-
Abz-KLLSSK-EDDnp
-
-
Abz-KLMSSK-EDDnp
-
-
Abz-KLNSSK-EDDnp
-
-
Abz-KLPSSK-EDDnp
-
-
Abz-KLQSSK-EDDnp
-
-
Abz-KLRSSK-EDDnp
-
-
Abz-KLSSSK-EDDnp
-
-
Abz-KLTSSK-EDDnp
-
-
Abz-KLVSSK-EDDnp
-
-
Abz-KLWSSK-EDDnp
-
-
Abz-KMFSSKQ-EDDnp
-
-
Abz-KNFSSKQ-EDDnp
-
-
Abz-KQFSSKQ-EDDnp
-
-
Abz-KRFSSKQ-EDDnp
-
-
Abz-KSFSSKQ-EDDnp
-
-
Abz-KTFSSKQ-EDDnp
-
-
Abz-KWFSSKQ-EDDnp
-
-
acetyl-D-Lys-L-Phe-L-Arg-D-Arg-L-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
-
acetyl-D-Lys-L-Phe-L-Arg-D-Arg-L-Gln-NH2
-
-
acetyl-D-Lys-L-Phe-L-Arg-D-Arg-NH2
-
-
acetyl-D-Lys-L-Phe-L-Arg-L-Leu-L-Glu-NH2
-
-
acetyl-Lys-Phe-Phe-Pro-Leu-Glu-NH2
Al3+
-
linear mixed inhibition
alpha1-antitrypsin
-
antipain
-
weak
Aprotinin
benzamidine
-
-
beta-mercaptoethanol
-
65% residual activity at 15 mM
Ca2+
-
linear competitive
Cd2+
-
-
convallatoxin
-
-
D-Pro-Phe-Arg-chloromethane
-
is an active-site-directed covalent inhibitor
digitoxigenin
-
-
digitoxin
-
-
diglutamic acid
-
-
digoxin
-
-
dihydrolipoate
-
35% residual activity at 3 mM
diisopropyl fluorophosphate
-
-
dithiothreitol
-
complete inhibition at 3 mM
DX-2300
-
DX-2300 is a fully human antibody that inhibits KLK1 via a competitive inhibition mechanism. Completely inhibits KLK1-like activity in urine and saliva, inhibits KLK1-like activity by 70% in bronchoalveolar lavage fluid. Inability of DX-2300 to bind either KLK1 covalently modified with 4-(2-aminoethyl)benzenesulfonyl fluoride or D-Pro-Phe-Arg-chloromethane, thus DX-2300 binds at or near the active site of the enzyme. DX-2300 blocks oxidative-stress-induced epidermal-growth-factor receptor activation and downstream mucus cell proliferation and hypersecretion, which is mediated by KLK1. DX-2300 is a potent and specific inhibitor of KLK1 that is efficacious in in vitro and in vivo models of airway disease
-
Egg white
-
-
-
fukugetin
-
mixed-type inhibitor
glutathione
-
16% residual activity at 15 mM
Human plasma
-
-
-
human urine
-
-
-
icatibant
-
blocks decrease of isoproterenol-induced cardiac hypertrophy and fibrosis by overexpressed tissue kallikrein in transgenic rats
K+
-
linear competitive
kallikrein antibody
-
-
-
kallistatin
-
leupeptin
-
weak
o-aminobenzoyl-AIKFFARE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFERE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFKRE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFLRE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFNRE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFPRE-N-(2,4-dinitrophenyl)ethylene diamine
-
-
o-aminobenzoyl-AIKFFRRE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSEE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSIE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSLE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSNE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSQE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSTE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSVE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFVRE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-D-Lys-L-Phe-L-Arg-D-Arg-L-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
-
o-aminobenzoyl-D-Lys-L-Phe-L-Arg-L-Pro-L-Arg-L-Gln N-(2,4-dinitrophenyl)ethylene diamine
-
-
o-aminobenzoyl-D-Lys-L-Phe-L-Phe-D-Arg-L-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
-
o-aminobenzoyl-D-Lys-L-Phe-L-Phe-L-Pro-L-Arg-L-Gln N-(2,4-dinitrophenyl)ethylene diamine
-
-
o-aminobenzoyl-D-Lys-L-Phe-L-Phe-L-Pro-L-Leu-L-Glu N-(2,4-dinitrophenyl)ethylene diamine
-
-
o-aminobenzoyl-F-4-(aminomethyl)cyclohexylalanine-SRQ-N-(2,4-dinitrophenyl)ethylene diamine
-
K1, highly specific
o-aminobenzoyl-TSVIRRPQ-ethylenediamine
-
-
Ouabain
-
-
peruvoside
-
-
phenylmethylsulfonyl fluoride
about 50% residual activity at 2.5 mM
PMSF
-
-
protease inhibitor-6
-
Protein C inhibitor
-
recombinant alpha1-antichymotrypsin
-
generation of selective inhibitors based on substrates selected from a pentapeptide phage-display library and transplanted to the reactive-site loop of alpha1-antichymotrypsin. Engineered alpha1-antichymotrypsins with peptides RGR-SE and ER-VSP are inhibitory and specific to enzyme.
-
serpin A1
about 40% residual activity at 0.002 mM
-
serpin F2
about 50% residual activity at 0.002 mM
-
tissue kallikrein-binding protein
-
rapidly forms a covalent complex with tissue kallikrein
-
tosyl-L-Lys-chloromethylketone
-
-
Trasylol
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
serpin A5
about 120% activity at 0.5-1.0 mM
-
Thermolysin
-
thermolysin activates prokallikrein cleaving off the N-terminal Ile1-Val2
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.27
Gln-Ala-Arg-7-amido-4-methylcoumarin
37°C, pH 7.5
1.18
Gly-Pro-Arg-7-amido-4-methylcoumarin
37°C, pH 7.5
0.44
Phe-Ser-Arg-7-amido-4-methylcoumarin
37°C, pH 7.5
0.53
Pro-Phe-Arg-7-amido-4-methylcoumarin
37°C, pH 7.5
0.28
Val-Pro-Arg-7-amido-4-methylcoumarin
37°C, pH 7.5
0.005
2-aminobenzoyl-TSVIRRVQ-ethylenediamine
-
-
-
0.0043
Abz-ALFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0032
Abz-FLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0015
Abz-FRSSR-Q-EDDnp
pH and temperature not specified in the publication
0.00036
Abz-GFSPFRSLRI-Q-EDDnp
pH and temperature not specified in the publication
0.0002
Abz-GFSPFRSSRI-Q-EDDnp
pH and temperature not specified in the publication
0.0062
Abz-GLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0034
Abz-HLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0027
Abz-ILFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0035
Abz-KAFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.003
Abz-KFFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0016
Abz-KLFFSK-EDDnp
-
at pH 7.5 and 37°C
0.0006
Abz-KLFRSK-EDDnp
-
at pH 7.5 and 37°C
0.0062
Abz-KLFSAKQ-EDDnp
-
at pH 7.5 and 37°C
0.0027
Abz-KLFSGKQ-EDDnp
-
at pH 7.5 and 37°C
0.0027
Abz-KLFSIKQ-EDDnp
-
at pH 7.5 and 37°C
0.0037
Abz-KLFSLKQ-EDDnp
-
at pH 7.5 and 37°C
0.0023
Abz-KLFSNKQ-EDDnp
-
at pH 7.5 and 37°C
0.0015
Abz-KLFSRKQ-EDDnp
-
at pH 7.5 and 37°C
0.0032
Abz-KLFSSDQ-EDDnp
-
at pH 7.5 and 37°C
0.0127
Abz-KLFSSEQ-EDDnp
-
at pH 7.5 and 37°C
0.0016
Abz-KLFSSGQ-EDDnp
-
at pH 7.5 and 37°C
0.0033
Abz-KLFSSIQ-EDDnp
-
at pH 7.5 and 37°C
0.0018
Abz-KLFSSK-EDDnp
-
at pH 7.5 and 37°C
0.0018
Abz-KLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0032
Abz-KLFSSLQ-EDDnp
-
at pH 7.5 and 37°C
0.0045
Abz-KLFSSNQ-EDDnp
-
at pH 7.5 and 37°C
0.0043
Abz-KLFSSQQ-EDDnp
-
at pH 7.5 and 37°C
0.0016
Abz-KLFSSRQ-EDDnp
-
at pH 7.5 and 37°C
0.0059
Abz-KLFSSSQ-EDDnp
-
at pH 7.5 and 37°C
0.0104
Abz-KLFSSVQ-EDDnp
-
at pH 7.5 and 37°C
0.0027
Abz-KLFSVKQ-EDDnp
-
at pH 7.5 and 37°C
0.0027
Abz-KLFTSK-EDDnp
-
at pH 7.5 and 37°C
0.0024
Abz-KLFWSK-EDDnp
-
at pH 7.5 and 37°C
0.00062 - 0.00065
Abz-KLRSSQ-EDDnp
0.0034
Abz-KLYSSK-EDDnp
-
at pH 7.5 and 37°C
0.0025
Abz-KPFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0019
Abz-KVFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0027
Abz-KYFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0046
Abz-LLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.009
Abz-MISLMKLP-Q-EDDnp
pH and temperature not specified in the publication
0.0007
Abz-MISLMKRP-Q-EDDnp
pH and temperature not specified in the publication
0.0017
Abz-NLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0052
Abz-QLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0041
Abz-RLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0021
Abz-VLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.14
acetyl-Phe-Arg ethyl ester
-
-
0.02
Ala-Arg-Arg-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
61.1
citrated bovine plasma
-
pH 7.8, 37°C
-
50.8
citrated dog plasma
-
pH 7.8, 37°C
-
0.0031
D-Pro-Phe-Arg-4-methylcoumarin-7-amide
-
37°C, pH 8.5
0.07
D-Pro-Phe-Phe-4-methylcoumarin-7-amide
-
37°C, pH 8.5
0.06 - 28.4
D-Val-Leu-Arg-p-nitroanilide
0.12 - 58.8
DL-Val-Leu-Arg-p-nitroanilide
17.6
human high molecular weight kininogen
-
pH 7.8, 37°C
-
12.7
human low molecular weight kininogen
-
pH 7.8, 37°C
-
0.071
Leu-Leu-Arg-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.00027
o-aminobenzoyl-AIKFFIRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00013
o-aminobenzoyl-AIKFFQRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.0018
o-aminobenzoyl-AIKFFSAE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00021
o-aminobenzoyl-AIKFFSKE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.0013
o-aminobenzoyl-AIKFFSPE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00036
o-aminobenzoyl-AIKFFSRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00094
o-aminobenzoyl-AIKFFSSE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00023
o-aminobenzoyl-AIKFFTRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.0002
o-aminobenzoyl-FRSSR-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.0009
o-aminobenzoyl-FRSVQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.0007
o-aminobenzoyl-GFSPF-4-(aminocyclohexyl)alanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.0038
o-aminobenzoyl-GFSPF-4-(aminomethyl)-N-isopropylphenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.0003
o-aminobenzoyl-GFSPF-4-(aminomethyl)phenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00064
o-aminobenzoyl-GFSPF-4-piperidinylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.001
o-aminobenzoyl-GFSPF-Nim-(dimethyl)histidine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.0067
o-aminobenzoyl-GFSPFRSSRIGEIKEEQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.0004
o-aminobenzoyl-GFSPFRSSRQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.00035
o-aminobenzoyl-GFSPFRSVQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.00041
o-aminobenzoyl-GFSPFRSVQVMKYEGSQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.0021
o-aminobenzoyl-GFSPFRSVTVQ-ethylenediamine 2,4-dinitrophenol
-
-
0.0023
o-aminobenzoyl-LMKRP-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.00735
o-aminobenzoyl-MTEMARRPQ-ethylenediamine 2,4-dinitrophenol
-
-
0.0036
o-aminobenzoyl-Phe-4-(aminomethyl)phenylalanine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.0007
o-aminobenzoyl-Phe-Nim-(dimethyl)histidine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.0042
o-aminobenzoyl-QPLGMISLMKRP-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.0455
o-aminobenzoyl-QPLGQTSLMKRP-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.07 - 0.114
Pro-Phe-Arg-4-methylcoumarin 7-amide
0.04
Pro-Phe-Arg-Arg-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.516 - 0.842
succinyl-Val-Pro-Phe-thiobenzyl ester
0.048
Val-Pro-Arg-Arg-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.067
Z-L-Phe-L-Arg-7-amido-4-methylcoumarin
-
pH 7.5, 25°C
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0038
Gln-Ala-Arg-7-amido-4-methylcoumarin
37°C, pH 7.5
0.0035
Gly-Pro-Arg-7-amido-4-methylcoumarin
37°C, pH 7.5
0.0065
Phe-Ser-Arg-7-amido-4-methylcoumarin
37°C, pH 7.5
0.031
Pro-Phe-Arg-7-amido-4-methylcoumarin
37°C, pH 7.5
0.0075
Val-Pro-Arg-7-amido-4-methylcoumarin
37°C, pH 7.5
0.1
Abz-ALFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.09
Abz-FLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
1.6
Abz-FRSSR-Q-EDDnp
pH and temperature not specified in the publication
4.6
Abz-GFSPFRSLRI-Q-EDDnp
pH and temperature not specified in the publication
17.7
Abz-GFSPFRSSRI-Q-EDDnp
pH and temperature not specified in the publication
0.03
Abz-GLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.1
Abz-HLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.06
Abz-ILFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.02
Abz-KAFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.02
Abz-KFFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.04
Abz-KLFFSK-EDDnp
-
at pH 7.5 and 37°C
0.04
Abz-KLFRSK-EDDnp
-
at pH 7.5 and 37°C
0.43
Abz-KLFSAKQ-EDDnp
-
at pH 7.5 and 37°C
0.04
Abz-KLFSGKQ-EDDnp
-
at pH 7.5 and 37°C
0.09
Abz-KLFSIKQ-EDDnp
-
at pH 7.5 and 37°C
0.15
Abz-KLFSLKQ-EDDnp
-
at pH 7.5 and 37°C
0.04
Abz-KLFSNKQ-EDDnp
-
at pH 7.5 and 37°C
0.15
Abz-KLFSRKQ-EDDnp
-
at pH 7.5 and 37°C
0.05
Abz-KLFSSDQ-EDDnp
-
at pH 7.5 and 37°C
0.5
Abz-KLFSSEQ-EDDnp
-
at pH 7.5 and 37°C
0.09
Abz-KLFSSGQ-EDDnp
-
at pH 7.5 and 37°C
0.02
Abz-KLFSSIQ-EDDnp
-
at pH 7.5 and 37°C
0.1
Abz-KLFSSK-EDDnp
-
at pH 7.5 and 37°C
0.1
Abz-KLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.08
Abz-KLFSSLQ-EDDnp
-
at pH 7.5 and 37°C
0.07
Abz-KLFSSNQ-EDDnp
-
at pH 7.5 and 37°C
0.14
Abz-KLFSSQQ-EDDnp
-
at pH 7.5 and 37°C
0.17
Abz-KLFSSRQ-EDDnp
-
at pH 7.5 and 37°C
0.43
Abz-KLFSSSQ-EDDnp
-
at pH 7.5 and 37°C
0.06
Abz-KLFSSVQ-EDDnp
-
at pH 7.5 and 37°C
0.35
Abz-KLFSVKQ-EDDnp
-
at pH 7.5 and 37°C
0.04
Abz-KLFTSK-EDDnp
-
at pH 7.5 and 37°C
0.04
Abz-KLFWSK-EDDnp
-
at pH 7.5 and 37°C
0.85
Abz-KLYSSK-EDDnp
-
at pH 7.5 and 37°C
0.02
Abz-KPFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.04
Abz-KVFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.02
Abz-KYFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.07
Abz-LLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.02
Abz-MISLMKLP-Q-EDDnp
pH and temperature not specified in the publication
0.33
Abz-MISLMKRP-Q-EDDnp
pH and temperature not specified in the publication
0.28
Abz-NLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.15
Abz-QLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.13
Abz-RLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.13
Abz-VLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.12
Ala-Arg-Arg-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
3.6
D-Pro-Phe-Arg-4-methylcoumarin-7-amide
-
37°C, pH 8.5
12.5
D-Pro-Phe-Phe-4-methylcoumarin-7-amide
-
37°C, pH 8.5
0.8 - 1.16
DL-Val-Leu-Arg-p-nitroanilide
0.04
Leu-Leu-Arg-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.05
o-aminobenzoyl-AIKFFIRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.02
o-aminobenzoyl-AIKFFQRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.54
o-aminobenzoyl-AIKFFSAE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.21
o-aminobenzoyl-AIKFFSKE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.05
o-aminobenzoyl-AIKFFSPE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
3.33
o-aminobenzoyl-AIKFFSRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.3
o-aminobenzoyl-AIKFFSSE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.04
o-aminobenzoyl-AIKFFTRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
1.4
o-aminobenzoyl-FRSSR-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.9
o-aminobenzoyl-FRSVQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.51
o-aminobenzoyl-GFSPF-4-(aminocyclohexyl)alanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.81
o-aminobenzoyl-GFSPF-4-(aminomethyl)-N-isopropylphenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
1.8
o-aminobenzoyl-GFSPF-4-(aminomethyl)phenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.61
o-aminobenzoyl-GFSPF-4-piperidinylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
2.1
o-aminobenzoyl-GFSPF-Nim-(dimethyl)histidine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
3
o-aminobenzoyl-GFSPFRSSRIGEIKEEQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
7.5
o-aminobenzoyl-GFSPFRSSRQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.94
o-aminobenzoyl-GFSPFRSVQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
1.5
o-aminobenzoyl-GFSPFRSVQVMKYEGSQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
3.1
o-aminobenzoyl-GFSPFRSVTVQ-ethylenediamine 2,4-dinitrophenol
-
-
0.04
o-aminobenzoyl-LMKRP-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
0.73
o-aminobenzoyl-MTEMARRPQ-ethylenediamine 2,4-dinitrophenol
-
-
1
o-aminobenzoyl-Phe-4-(aminomethyl)phenylalanine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
1.2
o-aminobenzoyl-Phe-Nim-(dimethyl)histidine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
7.1
o-aminobenzoyl-QPLGMISLMKRP-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
1.1
o-aminobenzoyl-QPLGQTSLMKRP-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 30°C
5.3 - 12.5
Pro-Phe-Arg-4-methylcoumarin 7-amide
0.917
Pro-Phe-Arg-Arg-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
2 - 29
succinyl-Val-Pro-Phe-thiobenzyl ester
0.0267
Val-Pro-Arg-Arg-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
additional information
additional information
-
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
22
Abz-ALFSSKQ-EDDnp
-
at pH 7.5 and 37°C
27
Abz-FLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
1067
Abz-FRSSR-Q-EDDnp
pH and temperature not specified in the publication
12780
Abz-GFSPFRSLRI-Q-EDDnp
pH and temperature not specified in the publication
88500
Abz-GFSPFRSSRI-Q-EDDnp
pH and temperature not specified in the publication
5
Abz-GLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
30
Abz-HLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
21
Abz-ILFSSKQ-EDDnp
-
at pH 7.5 and 37°C
6.3
Abz-KAFSSKQ-EDDnp
-
at pH 7.5 and 37°C
5.7
Abz-KFFSSKQ-EDDnp
-
at pH 7.5 and 37°C
25
Abz-KLFFSK-EDDnp
-
at pH 7.5 and 37°C
47
Abz-KLFRSK-EDDnp
-
at pH 7.5 and 37°C
69
Abz-KLFSAKQ-EDDnp
-
at pH 7.5 and 37°C
15
Abz-KLFSGKQ-EDDnp
-
at pH 7.5 and 37°C
31
Abz-KLFSIKQ-EDDnp
-
at pH 7.5 and 37°C
41
Abz-KLFSLKQ-EDDnp
-
at pH 7.5 and 37°C
16
Abz-KLFSNKQ-EDDnp
-
at pH 7.5 and 37°C
100
Abz-KLFSRKQ-EDDnp
-
at pH 7.5 and 37°C
16
Abz-KLFSSDQ-EDDnp
-
at pH 7.5 and 37°C
39
Abz-KLFSSEQ-EDDnp
-
at pH 7.5 and 37°C
53
Abz-KLFSSGQ-EDDnp
-
at pH 7.5 and 37°C
6
Abz-KLFSSIQ-EDDnp
-
at pH 7.5 and 37°C
55
Abz-KLFSSK-EDDnp
-
at pH 7.5 and 37°C
55
Abz-KLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
25
Abz-KLFSSLQ-EDDnp
-
at pH 7.5 and 37°C
16
Abz-KLFSSNQ-EDDnp
-
at pH 7.5 and 37°C
33
Abz-KLFSSQQ-EDDnp
-
at pH 7.5 and 37°C
106
Abz-KLFSSRQ-EDDnp
-
at pH 7.5 and 37°C
73
Abz-KLFSSSQ-EDDnp
-
at pH 7.5 and 37°C
6
Abz-KLFSSVQ-EDDnp
-
at pH 7.5 and 37°C
130
Abz-KLFSVKQ-EDDnp
-
at pH 7.5 and 37°C
15
Abz-KLFTSK-EDDnp
-
at pH 7.5 and 37°C
17
Abz-KLFWSK-EDDnp
-
at pH 7.5 and 37°C
250
Abz-KLYSSK-EDDnp
-
at pH 7.5 and 37°C
6.4
Abz-KPFSSKQ-EDDnp
-
at pH 7.5 and 37°C
20
Abz-KVFSSKQ-EDDnp
-
at pH 7.5 and 37°C
6
Abz-KYFSSKQ-EDDnp
-
at pH 7.5 and 37°C
15
Abz-LLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
2
Abz-MISLMKLP-Q-EDDnp
pH and temperature not specified in the publication
471
Abz-MISLMKRP-Q-EDDnp
pH and temperature not specified in the publication
165
Abz-NLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
29
Abz-QLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
32
Abz-RLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
62
Abz-VLFSSKQ-EDDnp
-
at pH 7.5 and 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003
Abz-KDFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0035
Abz-KEFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0026
Abz-KGFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0012
Abz-KHFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0005
Abz-KIFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0012
Abz-KKFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.002
Abz-KLASSK-EDDnp
-
at pH 7.5 and 37°C
0.0032
Abz-KLDSSK-EDDnp
-
at pH 7.5 and 37°C
0.003
Abz-KLESSK-EDDnp
-
at pH 7.5 and 37°C
0.0047
Abz-KLFESK-EDDnp
-
at pH 7.5 and 37°C
0.018
Abz-KLFHSK-EDDnp
-
at pH 7.5 and 37°C
0.0014
Abz-KLFISK-EDDnp
-
at pH 7.5 and 37°C
0.0006
Abz-KLFKSK-EDDnp
-
at pH 7.5 and 37°C
0.0014
Abz-KLFLSK-EDDnp
-
at pH 7.5 and 37°C
0.0083
Abz-KLFNSK-EDDnp
-
at pH 7.5 and 37°C
0.0014
Abz-KLFPSK-EDDnp
-
at pH 7.5 and 37°C
0.0061
Abz-KLFSEKQ-EDDnp
-
at pH 7.5 and 37°C
0.0096
Abz-KLFSFKQ-EDDnp
-
at pH 7.5 and 37°C
0.0014
Abz-KLFSHKQ-EDDnp
-
at pH 7.5 and 37°C
0.0048
Abz-KLFSPKQ-EDDnp
-
at pH 7.5 and 37°C
0.0009
Abz-KLFSQKQ-EDDnp
-
at pH 7.5 and 37°C
0.0017
Abz-KLFVSK-EDDnp
-
at pH 7.5 and 37°C
0.0022
Abz-KLGSSK-EDDnp
-
at pH 7.5 and 37°C
0.0013
Abz-KLHSSK-EDDnp
-
at pH 7.5 and 37°C
0.0002
Abz-KLISSK-EDDnp
-
at pH 7.5 and 37°C
0.0003
Abz-KLKSSK-EDDnp
-
at pH 7.5 and 37°C
0.0015
Abz-KLLSSK-EDDnp
-
at pH 7.5 and 37°C
0.001
Abz-KLMSSK-EDDnp
-
at pH 7.5 and 37°C
0.0011
Abz-KLNSSK-EDDnp
-
at pH 7.5 and 37°C
0.0022
Abz-KLPSSK-EDDnp
-
at pH 7.5 and 37°C
0.0022
Abz-KLQSSK-EDDnp
-
at pH 7.5 and 37°C
0.0016
Abz-KLRSSK-EDDnp
-
at pH 7.5 and 37°C
0.0018
Abz-KLSSSK-EDDnp
-
at pH 7.5 and 37°C
0.0023
Abz-KLTSSK-EDDnp
-
at pH 7.5 and 37°C
0.0017
Abz-KLVSSK-EDDnp
-
at pH 7.5 and 37°C
0.001
Abz-KLWSSK-EDDnp
-
at pH 7.5 and 37°C
0.0009
Abz-KMFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0041
Abz-KNFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0006
Abz-KQFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0011
Abz-KRFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0052
Abz-KSFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0007
Abz-KTFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.0009
Abz-KWFSSKQ-EDDnp
-
at pH 7.5 and 37°C
0.000424
acetyl-D-Lys-L-Phe-L-Arg-D-Arg-L-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.000494
acetyl-D-Lys-L-Phe-L-Arg-D-Arg-L-Gln-NH2
-
pH 9.0, 37°C
0.0044
acetyl-D-Lys-L-Phe-L-Arg-D-Arg-NH2
-
pH 9.0, 37°C
0.000029
acetyl-D-Lys-L-Phe-L-Arg-L-Leu-L-Glu-NH2
-
pH 9.0, 37°C
33
diglutamic acid
-
at pH 7.5 and 37°C
0.00000007
diisopropyl fluorophosphate
-
pH 7.2, 25°C
0.00000013
DX-2300
-
in 20 mM Tris/HCl buffer, pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.1% poly(ethylene glycol)-8000 and 0.1% Triton X-100
-
0.0016 - 0.0036
fukugetin
0.0000068
kallistatin
-
at pH 7.5 and 37°C
-
0.00033
o-aminobenzoyl-AIKFFARE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.0004
o-aminobenzoyl-AIKFFERE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00015
o-aminobenzoyl-AIKFFKRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00032
o-aminobenzoyl-AIKFFLRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00035
o-aminobenzoyl-AIKFFNRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00009
o-aminobenzoyl-AIKFFPRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00021
o-aminobenzoyl-AIKFFRRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00051
o-aminobenzoyl-AIKFFSEE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.001
o-aminobenzoyl-AIKFFSIE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00019
o-aminobenzoyl-AIKFFSLE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00073
o-aminobenzoyl-AIKFFSNE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.0006
o-aminobenzoyl-AIKFFSQE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00032
o-aminobenzoyl-AIKFFSTE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.0017
o-aminobenzoyl-AIKFFSVE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00022
o-aminobenzoyl-AIKFFVRE-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.000022
o-aminobenzoyl-D-Lys-L-Phe-L-Arg-D-Arg-L-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.000029
o-aminobenzoyl-D-Lys-L-Phe-L-Phe-D-Arg-L-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.00005
o-aminobenzoyl-F-4-(aminomethyl)cyclohexylalanine-SRQ-N-(2,4-dinitrophenyl)ethylene diamine
-
pH 9.0, 37°C
0.0042
o-aminobenzoyl-TSVIRRPQ-ethylenediamine
-
pH 9.0, 37°C
0.000000025
Protein C inhibitor
-
pH 7.5, 37°C
-
0.0033
Zn2+-dependent small-molecule competitive inhibitor
-
-
-
additional information
additional information
-
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00013 - 0.00015
convallatoxin
0.00021 - 0.00038
digitoxigenin
0.000042 - 0.00005
digitoxin
0.00009 - 0.0001
digoxin
0.0032 - 0.0057
fukugetin
0.000065 - 0.00012
Ouabain
0.000006 - 0.000008
peruvoside
0.001
serpin A1
Homo sapiens
at pH 8.0 and 37°C
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47
-
purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
hydrolysis of Pro-Phe-Arg-7-amido-4-methylcoumarin
7.8
-
assay at
8 - 10
-
cleavage of D-Val-Leu-Arg-p-nitroanilide
8.2
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9.5
-
pH 6.5: about 30% of maximal activity, pH 9.5: about 40% of maximal activity
7 - 10
about 35% activity at pH 7.0, about 90% activity at pH 8.0, about 55% activity at pH 9.0, about 35% activity at pH 10.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at
30
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
secretory epithelial cell
Manually annotated by BRENDA team
-
secretory epithelial cells tf trachea, thyroid gland, mammary gland, salivary gland, jejunum, ileum, epididymis, seminal vesicle and urethra
Manually annotated by BRENDA team
-
secretory epithelial cell
Manually annotated by BRENDA team
-
secretory epithelial cell
Manually annotated by BRENDA team
-
granular keratinocyte
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
secretory epithelial cell
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
low content
Manually annotated by BRENDA team
lower content
Manually annotated by BRENDA team
-
KLK4 and KLK11
Manually annotated by BRENDA team
low content
Manually annotated by BRENDA team
-
secretory epithelial cell
Manually annotated by BRENDA team
-
secretory epithelial cell
Manually annotated by BRENDA team
-
secretory epithelial cell
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KLK11_HUMAN
282
0
31059
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
x * 40000, SDS-PAGE
14000
-
x * 14000 + x * 18000, SDS-PAGE under reducing conditions
18000
-
x * 14000 + x * 18000, SDS-PAGE under reducing conditions
29000
-
x * 29000, SDS-PAGE under reducing conditions
32000
-
x * 32000, K2, SDS-PAGE
34000
-
x * 34000 or x * 37000, the two molecular species represent differentially glycosylated forms
37000
-
x * 34000 or x * 37000, the two molecular species represent differentially glycosylated forms
40000
-
gel filtration
40500
-
short column equilibrium sedimentation
46000
-
1 * 46000, active kallikrein, SDS-PAGE
48000
-
1 * 48000, prokallikrein, SDS-PAGE
53000
-
prokallikrein and kallikrein, gel filtration
additional information
about 40% of human tissue kallikrein 11 (40000 Da) is enzymatically active, whereas the rest is inactivated by internal cleavage after Arg156 which generates two peptides of 20000 Da, connected by internal disulfide bonds
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 40000, SDS-PAGE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
proteolytic modification
side-chain modification
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme expressed in a deglycosylated form at high levels in Pichia pastoris, structure analysis
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R53H
-
inactive
Y228A
-
enlarges the S1 pocket
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2
-
immediate irreversible inactivation
647503
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for more than 3 months
-
-30°C, purified urinary enzyme, stable for 1 year
-
20°C, purified urinary enzyme, 0.02% w/v NaN3, neutral pH, stable for more than 14 days
-
4°C, purified urinary enzyme, 0.02% w/v NaN3, stable for more than 6 months
-
4°C, stable for 1 month
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1460fold
-
397fold
-
aprotinin-CH-Sepharose column chromatography and gel filtration
-
by affinity column chromatography
-
enzyme expressed in a deglycosylated form at high levels in Pichia pastoris
-
from urine
-
glandular kallikrein, 5000fold, to homogeneity
-
HiTrap Q column chromatography
large scale
-
prokallikrein, 183fold
-
recombinant
-
recombinant from Pichia pastoris
-
to homogeneity
-
Zn2+-nitrilotriacetic acid agarose column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
860-bp fragment of tissue kallikrein containing an open reading frame subcloned into pXXUF1 downstream of CMV. recombinant adeno-associated virus encoding tissue kallikrein delivered to spontaneously hypertensive rats and cultured HEK-293 cells
-
cDNA of the tissue kallikrein gene is subcloned into the chicken oviduct-specific expression vector pOV3. The resultant recombinant vector pOV3K is injected into laying hens via wing vein after mixing with polyethyleneimine. The chicken oviduct-specific transient expression system can produce relatively high level and authentic recombinant enzyme with a potential for further development for therapeutic use
-
DNA sequence determination, expression of prokallikrein in Pichia pastoris
-
expressed in a baculovirus/insect cell line system
-
expressed in Chlamydomonas reinhardtii
-
expressed in diabetic Rattus norvegicus
-
expressed in Expi293 cells
expressed in ischemic mouse heart
-
expressed in Mus musculus and Danio rerio
-
expressed in Mus musculus and Rattus norvegicus
-
expressed in Rattus norvegicus and Mus musculus myocardium
-
expressed in Rattus norvegicus heart
-
expressed in the egg white of laying hens
-
expression in Escherichia coli
-
expression of recombinant human prokallikrein and kallikrein in engineered Chinese hamster ovary cells
-
genetic organization and structure of the kallikrein gene family, clustered on chromosome 19q13.3-q13.4
overexpressed in transgenic mice or rats
-
production of pro-form of tissue kallikrein 9 in Escherichia coli cells. Production of tissue kallikrein 9 in CHO cells
-
recombinant adeno-associated virus-mediated human tissue kallikrein expression is induced in a rodent model of chronic renal failure
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
aprotinin attenuates high-glucose stimulated expression of KLK1
-
blood plasma tissue kallikrein levels are significantly lower in stroke patients, especially those with ischemic stroke
-
in an endothelial-cervical carcinoma conditioned-medium model, HeLa metabolites cause a dramatic decrease in endothelial cellular tissue kallikrein and a concomitant proliferation of endothelial cells
-
lower human kallikrein protein levels are observed in proangiogenic cells from type 2 diabetic patients
-
patients with coronary artery disease and acute coronary syndrome have significantly elevated KLK1 levels
-
presence of tissue kallikrein in HeLa cells and in angiogenic HUVECs. In the submandibular gland control tissue, positive tissue kallikrein staining within the ductal epithelial gland. On conditioned media a dose-dependent increase of tissue kallikrein. In challenged endothelial cells with metabolites from cervical cancer cells, the reduction in cellular tissue kallikrein is associated with a concomitant increase in tissue kallikrein within the media
-
tissue kallikrein (KLK1) expression is up-regulated in human diabetic kidney tissue and induced by high glucose in human proximal tubular epithelial cells
-
tubular expression of KLK1 in diabetic kidney biopsies. High-glucose stimulates expression of KLK1
-
urinary kallikrein excretion is significantly reduced in patients with mild renal disease, and to a further extent in patients with severe renal failure
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
-
the chicken oviduct-specific transient expression system can produce relatively high level of authentic recombinant enzyme with a potential for further development for therapeutic use
drug development
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Homo sapiens (P06870), Macaca fascicularis (Q07276), Mastomys natalensis (P32824), Mus musculus (P00755), Papio hamadryas (Q28773), Rattus norvegicus (P00758)
Manually annotated by BRENDA team
Yousef, G.M.; Diamandis, E.P.
The new human tissue kallikrein gene family: structure, function, and association to disease
Endocr. Rev.
22
184-204
2001
Homo sapiens, Homo sapiens (P06870)
Manually annotated by BRENDA team
El Moujahed, A.; Brillard-Bourdet, M.; Juliano, M.A.; Moreau, T.; Chagas, J.R.; Gutman, N.; Prado, E.S.; Gauthier, F.
Kininogen-derived fluorogenic substrates for investigating the vasoactive properties of rat kallikreins. Identification of a T-kinin-releasing rat kallikrein
Eur. J. Biochem.
247
652-658
1997
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Hofmann, W.; Junk, A.; Geiger, R.
Human tissue kallikrein. II. Isolation and characterization of human salivary kallikrein
Hoppe-Seyler's Z. Physiol. Chem.
364
425-432
1983
Homo sapiens
Manually annotated by BRENDA team
Webster, M.E.; Prado, E.S.
Glandular kallikreins from horse and human urine and from hog pancreas
Methods Enzymol.
19
681-699
1970
Equus caballus, Homo sapiens, Sus scrofa
-
Manually annotated by BRENDA team
Geiger, R.; Clausnitzer, B.; Fink, H.; Fritz, H.
Isolation of an enzymatically active glandular kallikrein from human plasma by immunoaffinity chromatography
Hoppe-Seyler's Z. Physiol. Chem.
361
179-1803
1980
Homo sapiens
-
Manually annotated by BRENDA team
Takada, Y.; Skidgel, R.A.; Erds, E.G.
Purification of human urinary prokallikrein
Biochem. J.
232
851-858
1985
Homo sapiens
Manually annotated by BRENDA team
Geiger, R.; Clausnitzer, B.
Isolation of an enzymatically active tissue kallikrein from human seminal plasma by immunoaffinity chromatography
Hoppe-Seyler's Z. Physiol. Chem.
362
1279-1283
1981
Homo sapiens
Manually annotated by BRENDA team
Pimenta, D.C.; Fogaca, S.E.; Melo, R.L.; Juliano, L.; Juliano, M.A.
Specificity of S'1 and S'2 subsites of human tissue kallikrein using the reactive-centre loop of kallistatin: the importance of P'1 and P'2 positions in design of inhibitors
Biochem. J.
371
1021-1025
2003
Homo sapiens
Manually annotated by BRENDA team
Girolami, J.P.; Pecher, C.; Bascands, J.L.; Cabos, G.; Adam, A.; Suc, J.M.
Purification of human active urinary kallikrein: comparative inhibition studies of kininogenase and amidolytic activities
Prep. Biochem.
19
175-190
1989
Homo sapiens
-
Manually annotated by BRENDA team
Chan, H.; Springman, E.B.; Clark, J.M.
Expression and characterization of human tissue kallikrein variants
Protein Expr. Purif.
12
361-370
1998
Homo sapiens
Manually annotated by BRENDA team
Fujimoto, Y.; Suzuki, C.; Watanabe, Y.; Matsuda, Y.; Akihama, S.
Purification and characterization of a kallikrein from human submaxillary glands
Biochem. Med. Metab. Biol.
44
218-227
1990
Homo sapiens
Manually annotated by BRENDA team
Lu, H.S.; Hsu, Y.R.; Narhi, L.O.; Karkare, S.; Lin, F.K.
Purification and characterization of human tissue prokallikrein and kallikrein isoforms expressed in Chinese hamster ovary cells
Protein Expr. Purif.
8
227-237
1996
Homo sapiens
Manually annotated by BRENDA team
Lu, H.S.; Hsu, Y.R.; Lu, L.I.; Ruff, D.; Lyons, D.; Lin, F.K.
Isolation and characterization of human tissue kallikrein produced in Escherichia coli: biochemical comparison to the enzymatically inactive prokallikrein and methionyl kallikrein
Protein Expr. Purif.
8
215-226
1996
Homo sapiens
Manually annotated by BRENDA team
Geiger, R.; Miska, W.
Human tissue kallikrein
Methods Enzymol.
163
102-115
1988
Homo sapiens
Manually annotated by BRENDA team
Jones, T.H.; Figueroa, C.D.; Smith, C.; Cullen, D.R.; Bhoola, K.D.
Characterization of a tissue kallikrein in human prolactin-secreting aenomas
J. Endocrinol.
124
327-331
1990
Homo sapiens
Manually annotated by BRENDA team
Oliva, M.L.; Mendes, C.R.; Juliano, M.A.; Chagas, J.R.; Rosa, J.C.; Greene, L.J.; Sampaio, M.U.; Sampaio, C.A.
Characterization of a tissue kallikrein inhibitor isolated from Bauhinia bauhinioides seeds: inhibition of the hydrolysis of kininogen related substrates
Immunopharmacology
45
163-169
1999
Homo sapiens, Sus scrofa
Manually annotated by BRENDA team
Del Nery, E.; Chagas, J.R.; Juliano, M.A.; Juliano, L.; Prado, E.S.
Comparison of human and porcine tissue kallikrein substrate specificities
Immunopharmacology
45
151-157
1999
Homo sapiens, Sus scrofa
Manually annotated by BRENDA team
Pimenta, D.C.; Chao, J.; Chao, L.; Juliano, M.A.; Juliano, L.
Specificity of human tissue kallikrein towards substrates containing Phe-Phe pair of amino acids
Biochem. J.
339
473-479
1999
Homo sapiens
Manually annotated by BRENDA team
Chen, V.C.; Chao, L.; Chao, J.
Roles of the P1, P2, and P3 residues in determining inhibitory specificity of kallistatin toward human tissue kallikrein
J. Biol. Chem.
275
38457-38466
2000
Homo sapiens
Manually annotated by BRENDA team
Chen, V.C.; Chao, L.; Chao, J.
A positively charged loop on the surface of kallistatin functions to enhance tissue kallikrein inhibition by acting as a secondary binding site for kallikrein
J. Biol. Chem.
275
40371-40377
2000
Homo sapiens
Manually annotated by BRENDA team
Melo, R.L.; Barbosa Pozzo, R.C.; Pimenta, D.C.; Perissutti, E.; Caliendo, G.; Santagada, V.; Juliano, L.; Juliano, M.A.
Human tissue kallikrein S1 subsite recognition of non-natural basic amino acids
Biochemistry
40
5226-5232
2001
Homo sapiens
Manually annotated by BRENDA team
Clements, J.; Hooper, J.; Dong, Y.; Harvey, T.
The expanded human kallikrein (KLK) gene family: genomic organisation, tissue-specific expression and potential functions
Biol. Chem.
382
5-14
2001
Homo sapiens (P06870)
Manually annotated by BRENDA team
Sakamoto, W.; Yoshikawa, K.; Uehara, S.; Nishikaze, O.
Characterization of human salivary kallikrein: reactivities to human plasma kininogens and proteinase inhibitors
J. Biochem.
93
833-838
1983
Homo sapiens
Manually annotated by BRENDA team
Katz, B.A.; Liu, B.; Barnes, M.; Springman, E.B.
Crystal structure of recombinant human tissue kallikrein at 2.0 A resolution
Protein Sci.
7
875-885
1998
Homo sapiens
Manually annotated by BRENDA team
Lvgren, J.; Airas, K.; Lilja, H.
Enzymatic action of human glandular kallikrein 2 (hK2). Substrate specificity and regulation by Zn2+ and extracellular protease inhibitors
Eur. J. Biochem.
262
781-789
1999
Homo sapiens
Manually annotated by BRENDA team
Charlesworth, M.C.; Young, C.Y.; Miller, V.M.; Tindall, D.J.
Kininogenase activity of prostate-derived human glandular kallikrein (hK2) purified from seminal fluid
J. Androl.
20
220-229
1999
Homo sapiens
Manually annotated by BRENDA team
Saedi, M.S.; Zhu, Z.; Marker, K.; Liu, R.S.; Carpenter, P.M.; Rittenhouse, H.; Mikolajczyk, S.D.
Human kallikrein 2 (hK2), but not prostate-specific antigen (PSA), rapidly complexes with protease inhibitor 6 (PI-6) released from prostate carcinoma cells
Int. J. Cancer
94
558-563
2001
Homo sapiens
Manually annotated by BRENDA team
Rehault, S.; Monget, P.; Mazerbourg, S.; Tremblay, R.; Gutman, N.; Gauthier, F.; Moreau, T.
Insulin-like growth factor binding proteins (IGFBPs) as potential physiological substrates for human kallikreins hK2 and hK3
Eur. J. Biochem.
268
2960-2968
2001
Homo sapiens
Manually annotated by BRENDA team
Yousef, G.M.; Diamandis, E.P.
Expanded human tissue kallikrein family-a novel panel of cancer biomarkers
Tumour Biol.
23
185-192
2002
Homo sapiens
Manually annotated by BRENDA team
Cloutier, S.M.; Chagas, J.R.; Mach, J.P.; Gygi, C.M.; Leisinger, H.J.; Deperthes, D.
Substrate specificity of human kallikrein 2 (hK2) as determined by phage display technology
Eur. J. Biochem.
269
2747-2754
2002
Homo sapiens
Manually annotated by BRENDA team
Beaufort, N.; Debela, M.; Creutzburg, S.; Kellermann, J.; Bode, W.; Schmitt, M.; Pidard, D.; Magdolen, V.
Interplay of human tissue kallikrein 4 (hK4) with the plasminogen activation system: hK4 regulates the structure and functions of the urokinase-type plasminogen activator receptor (uPAR)
Biol. Chem.
387
217-222
2006
Homo sapiens
Manually annotated by BRENDA team
Olsson, A.Y.; Bjartell, A.; Lilja, H.; Lundwall, A.
Expression of prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2) in ileum and other extraprostatic tissues
Int. J. Cancer
113
290-297
2005
Homo sapiens
Manually annotated by BRENDA team
Fogaca, S.E.; Melo, R.L.; Pimenta, D.C.; Hosoi, K.; Juliano, L.; Juliano, M.A.
Differences in substrate and inhibitor sequence specificity of human, mouse and rat tissue kallikreins
Biochem. J.
380
775-781
2004
Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Memari, N.; Grass, L.; Nakamura, T.; Karakucuk, I.; Diamandis, E.P.
Human tissue kallikrein 9: production of recombinant proteins and specific antibodies
Biol. Chem.
387
733-740
2006
Homo sapiens
Manually annotated by BRENDA team
Rosatelli, T.B.; Roselino, A.M.; Dellalibera-Joviliano, R.; Reis, M.L.; Donadi, E.A.
Increased activity of plasma and tissue kallikreins, plasma kininase II and salivary kallikrein in pemphigus foliaceus (fogo selvagem)
Br. J. Dermatol.
152
650-657
2005
Homo sapiens
Manually annotated by BRENDA team
Luo, L.Y.; Shan, S.J.; Elliott, M.B.; Soosaipillai, A.; Diamandis, E.P.
Purification and characterization of human kallikrein 11, a candidate prostate and ovarian cancer biomarker, from seminal plasma
Clin. Cancer Res.
12
742-750
2006
Homo sapiens (Q9UBX7)
Manually annotated by BRENDA team
Cloutier, S.M.; Kuendig, C.; Felber, L.M.; Fattah, O.M.; Chagas, J.R.; Gygi, C.M.; Jichlinski, P.; Leisinger, H.J.; Deperthes, D.
Development of recombinant inhibitors specific to human kallikrein 2 using phage-display selected substrates
Eur. J. Biochem.
271
607-613
2004
Homo sapiens
Manually annotated by BRENDA team
Steuber, T.; Vickers, A.J.; Haese, A.; Becker, C.; Pettersson, K.; Chun, F.K.; Kattan, M.W.; Eastham, J.A.; Scardino, P.T.; Huland, H.; Lilja, H.
Risk assessment for biochemical recurrence prior to radical prostatectomy: significant enhancement contributed by human glandular kallikrein 2 (hK2) and free prostate specific antigen (PSA) in men with moderate PSA-elevation in serum
Int. J. Cancer
118
1234-1240
2006
Homo sapiens
Manually annotated by BRENDA team
Casalino-Matsuda, S.M.; Monzon, M.E.; Conner, G.E.; Salathe, M.; Forteza, R.M.
Role of hyaluronan and reactive oxygen species in tissue kallikrein-mediated epidermal growth factor receptor activation in human airways
J. Biol. Chem.
279
21606-21616
2004
Homo sapiens
Manually annotated by BRENDA team
Azizi, M.; Boutouyrie, P.; Bissery, A.; Agharazii, M.; Verbeke, F.; Stern, N.; Bura-Riviere, A.; Laurent, S.; Alhenc-Gelas, F.; Jeunemaitre, X.
Arterial and renal consequences of partial genetic deficiency in tissue kallikrein activity in humans
J. Clin. Invest.
115
780-787
2005
Homo sapiens
Manually annotated by BRENDA team
De Sousa, M.O.; Santoro, M.M.; De Souza Figueiredo, A.F.
The effect of cations on the amidase activity of human tissue kallikrein: 1-linear competitive inhibition by sodium, potassium, calcium and magnesium. 2-linear mixed inhibition by aluminium
J. Enzyme Inhib. Med. Chem.
19
317-325
2004
Homo sapiens
Manually annotated by BRENDA team
Komatsu, N.; Tsai, B.; Sidiropoulos, M.; Saijoh, K.; Levesque, M.A.; Takehara, K.; Diamandis, E.P.
Quantification of eight tissue kallikreins in the Stratum corneum and sweat
J. Invest. Dermatol.
126
925-929
2006
Homo sapiens
Manually annotated by BRENDA team
Borgono, C.A.; Michael, I.P.; Diamandis, E.P.
Human tissue kallikreins: physiologic roles and applications in cancer
Mol. Cancer Res.
2
257-280
2004
Homo sapiens
Manually annotated by BRENDA team
Gao, B.; Sun, H.C.; Fang, H.X.; Qian, K.; Zhao, M.S.; Qiu, H.L.; Song, C.Y.; Wang, Z.Y.
Expression and preliminary characterization of recombinant human tissue kallikrein in egg white of laying hens
Poult. Sci.
85
1239-1244
2006
Homo sapiens
Manually annotated by BRENDA team
Sharma, J.N.
Role of tissue kallikrein-kininogen-kinin pathways in the cardiovascular system
Arch. Med. Res.
37
299-306
2006
Homo sapiens
Manually annotated by BRENDA team
Pampalakis, G.; Sotiropoulou, G.
Tissue kallikrein proteolytic cascade pathways in normal physiology and cancer
Biochim. Biophys. Acta
1776
22-31
2007
Homo sapiens
Manually annotated by BRENDA team
Paliouras, M.; Diamandis, E.P.
The kallikrein world: an update on the human tissue kallikreins
Biol. Chem.
387
643-652
2006
Homo sapiens
Manually annotated by BRENDA team
Chao, J.; Bledsoe, G.; Yin, H.; Chao, L.
The tissue kallikrein-kinin system protects against cardiovascular and renal diseases and ischemic stroke independently of blood pressure reduction
Biol. Chem.
387
665-675
2006
Homo sapiens
Manually annotated by BRENDA team
Paliouras, M.; Borgono, C.; Diamandis, E.P.
Human tissue kallikreins: the cancer biomarker family
Cancer Lett.
249
61-79
2007
Homo sapiens
Manually annotated by BRENDA team
Li, H.J.; Yin, H.; Yao, Y.Y.; Shen, B.; Bader, M.; Chao, L.; Chao, J.
Tissue kallikrein protects against pressure overload-induced cardiac hypertrophy through kinin B2 receptor and glycogen synthase kinase-3beta activation
Cardiovasc. Res.
73
130-142
2007
Homo sapiens
Manually annotated by BRENDA team
Yuan, G.; Deng, J.; Wang, T.; Zhao, C.; Xu, X.; Wang, P.; Voltz, J.W.; Edin, M.L.; Xiao, X.; Chao, L.; Chao, J.; Zhang, X.A.; Zeldin, D.C.; Wang, D.W.
Tissue kallikrein reverses insulin resistance and attenuates nephropathy in diabetic rats by activation of phosphatidylinositol 3-kinase/protein kinase B and adenosine 5-monophosphate-activated protein kinase signaling pathways
Endocrinology
148
2016-2026
2007
Homo sapiens
Manually annotated by BRENDA team
Scarisbrick, I.A.; Blaber, S.I.; Tingling, J.T.; Rodriguez, M.; Blaber, M.; Christophi, G.P.
Potential scope of action of tissue kallikreins in CNS immune-mediated disease
J. Neuroimmunol.
178
167-176
2006
Homo sapiens (P06870)
Manually annotated by BRENDA team
Naidoo, S.; Raidoo, D.M.
Tissue kallikrein and kinin receptor expression in an angiogenic co-culture neuroblastoma model
Metab. Brain Dis.
21
253-265
2006
Homo sapiens
Manually annotated by BRENDA team
Madeddu, P.; Emanueli, C.; El-Dahr, S.
Mechanisms of disease: the tissue kallikrein-kinin system in hypertension and vascular remodeling
Nat. Clin. Pract. Nephrol.
3
208-221
2007
Homo sapiens
Manually annotated by BRENDA team
Stone, O.A.; Richer, C.; Emanueli, C.; van Weel, V.; Quax, P.H.; Katare, R.; Kraenkel, N.; Campagnolo, P.; Barcelos, L.S.; Siragusa, M.; Sala-Newby, G.B.; Baldessari, D.; Mione, M.; Vincent, M.P.; Benest, A.V.; Al Haj Zen, A.; Gonzalez, J.; Bates, D.O.; Alhenc-Gelas, F.; Madeddu, P.
Critical role of tissue kallikrein in vessel formation and maturation. Implications for therapeutic revascularization
Arterioscler. Thromb. Vasc. Biol.
29
657-664
2009
Homo sapiens
Manually annotated by BRENDA team
Weber, W.; Buck, F.; Meyer, A.; Hilz, H.
Prostate specific antigen: one out of five disulfide bridges determines inactivation by reduction
Biochem. Biophys. Res. Commun.
379
1101-1106
2009
Homo sapiens
Manually annotated by BRENDA team
Clements, J.A.
Reflections on the tissue kallikrein and kallikrein-related peptidase family - from mice to men - what have we learnt in the last two decades?
Biol. Chem.
389
1447-1454
2008
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Lima, A.R.; Alves, F.M.; Angelo, P.F.; Andrade, D.; Blaber, S.I.; Blaber, M.; Juliano, L.; Juliano, M.A.
S(1) and S(2) subsite specificities of human plasma kallikrein and tissue kallikrein 1 for the hydrolysis of peptides derived from the bradykinin domain of human kininogen
Biol. Chem.
389
1487-1494
2008
Homo sapiens
Manually annotated by BRENDA team
Eissa, A.; Diamandis, E.P.
Human tissue kallikreins as promiscuous modulators of homeostatic skin barrier functions
Biol. Chem.
389
669-680
2008
Homo sapiens
Manually annotated by BRENDA team
Pizard, A.; Richer, C.; Bouby, N.; Picard, N.; Meneton, P.; Azizi, M.; Alhenc-Gelas, F.
Genetic deficiency in tissue kallikrein activity in mouse and man: effect on arteries, heart and kidney
Biol. Chem.
389
701-706
2008
Homo sapiens
Manually annotated by BRENDA team
Yao, Y.Y.; Yin, H.; Shen, B.; Smith, R.S.; Liu, Y.; Gao, L.; Chao, L.; Chao, J.
Tissue kallikrein promotes neovascularization and improves cardiac function by the Akt-glycogen synthase kinase-3beta pathway
Cardiovasc. Res.
80
354-364
2008
Homo sapiens
Manually annotated by BRENDA team
Prassas, I.; Paliouras, M.; Datti, A.; Diamandis, E.P.
High-throughput screening identifies cardiac glycosides as potent inhibitors of human tissue kallikrein expression: implications for cancer therapies
Clin. Cancer Res.
14
5778-5784
2008
Homo sapiens
Manually annotated by BRENDA team
Tu, L.; Xu, X.; Wan, H.; Zhou, C.; Deng, J.; Xu, G.; Xiao, X.; Chen, Y.; Edin, M.L.; Voltz, J.W.; Zeldin, D.C.; Wang, D.W.
Delivery of recombinant adeno-associated virus-mediated human tissue kallikrein for therapy of chronic renal failure in rats
Hum. Gene Ther.
19
318-330
2008
Homo sapiens
Manually annotated by BRENDA team
Christiansen, S.C.; Eddleston, J.; Bengtson, S.H.; Jenkins, G.R.; Sarnoff, R.B.; Turner, R.B.; Gwaltney, J.M.; Zuraw, B.L.
Experimental rhinovirus infection increases human tissue kallikrein activation in allergic subjects
Int. Arch. Allergy Immunol.
147
299-304
2008
Homo sapiens
Manually annotated by BRENDA team
Silva, J.A.; Silva, M.R.; Nascimento, J.W.; Bader, M.; Pesquero, J.B.
Protective actions of human tissue kallikrein gene in transgenic rat hearts
Int. Immunopharmacol.
8
267-270
2008
Homo sapiens
Manually annotated by BRENDA team
Wenske, S.; Korets, R.; Cronin, A.M.; Vickers, A.J.; Fleisher, M.; Scher, H.I.; Pettersson, K.; Guillonneau, B.; Scardino, P.T.; Eastham, J.A.; Lilja, H.
Evaluation of molecular forms of prostate-specific antigen and human kallikrein 2 in predicting biochemical failure after radical prostatectomy
Int. J. Cancer
124
659-663
2009
Homo sapiens
Manually annotated by BRENDA team
Azizi, M.; Emanueli, C.; Peyrard, S.; Maddedu, P.; Alhenc-Gelas, F.; Campbell, D.J.
Genetic and dietary control of plasma tissue kallikrein secretion and urinary kinins exretion in man
J. Hypertens.
26
714-720
2008
Homo sapiens
Manually annotated by BRENDA team
Chiang, W.C.; Lin, S.L.; Chen, Y.M.; Wu, K.D.; Tsai, T.J.
Urinary kallikrein excretion is related to renal function change and inflammatory status in chronic kidney disease patients receiving angiotensin II receptor blocker treatment
Nephrology
13
198-203
2008
Homo sapiens
Manually annotated by BRENDA team
Wang, A.P.; Sun, H.C.; Wang, J.Y.; Wang, Y.J.; Yuan, W.F.
Recombinant avian adeno-associated virus-mediated oviduct-specific expression of recombinant human tissue kallikrein
Poult. Sci.
87
777-782
2008
Homo sapiens
Manually annotated by BRENDA team
Yan, J.T.; Wang, T.; Wang, D.W.
Recombinant adeno-associated virus-mediated human kallikrein gene therapy protects against hypertensive target organ injuries through inhibiting cell apoptosis
Acta Pharmacol. Sin.
30
1253-1261
2009
Homo sapiens
Manually annotated by BRENDA team
Sexton, D.J.; Chen, T.; Martik, D.; Kuzmic, P.; Kuang, G.; Chen, J.; Nixon, A.E.; Zuraw, B.L.; Forteza, R.M.; Abraham, W.M.; Wood, C.R.
Specific inhibition of tissue kallikrein 1 with a human monoclonal antibody reveals a potential role in airway diseases
Biochem. J.
422
383-392
2009
Ovis aries, Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Chao, J.; Shen, B.; Gao, L.; Xia, C.F.; Bledsoe, G.; Chao, L.
Tissue kallikrein in cardiovascular, cerebrovascular and renal diseases and skin wound healing
Biol. Chem.
391
345-355
2010
Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Gao, L.; Smith, R.S.; Chen, L.M.; Chai, K.X.; Chao, L.; Chao, J.
Tissue kallikrein promotes prostate cancer cell migration and invasion via protease-activated receptor-1-dependent signaling pathway
Biol. Chem.
391
803-812
2010
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Liu, L.; Zhang, R.; Liu, K.; Zhou, H.; Yang, X.; Liu, X.; Tang, M.; Su, J.; Dong, Q.
Tissue kallikrein protects cortical neurons against in vitro ischemia-acidosis/reperfusion-induced injury through the ERK1/2 pathway
Exp. Neurol.
219
453-465
2009
Homo sapiens
Manually annotated by BRENDA team
Chen, Z.B.; Huang, D.Q.; Niu, F.N.; Zhang, X.; Li, E.G.; Xu, Y.
Human urinary kallidinogenase suppresses cerebral inflammation in experimental stroke and downregulates nuclear factor-kappaB
J. Cereb. Blood Flow Metab.
30
1356-1365
2010
Homo sapiens
Manually annotated by BRENDA team
Liu, L.; Zhang, R.; Liu, K.; Zhou, H.; Tang, Y.; Su, J.; Yu, X.; Yang, X.; Tang, M.; Dong, Q.
Tissue kallikrein alleviates glutamate-induced neurotoxicity by activating ERK1
J. Neurosci. Res.
87
3576-3590
2009
Homo sapiens
Manually annotated by BRENDA team
Tang, S.C.; Chan, L.Y.; Leung, J.C.; Cheng, A.S.; Chan, K.W.; Lan, H.Y.; Lai, K.N.
Bradykinin and high glucose promote renal tubular inflammation
Nephrol. Dial. Transplant.
25
698-710
2010
Homo sapiens
Manually annotated by BRENDA team
Naidoo, S.; Raidoo, D.M.
Angiogenesis in cervical cancer is mediated by HeLa metabolites through endothelial cell tissue kallikrein
Oncol. Rep.
22
285-293
2009
Homo sapiens
Manually annotated by BRENDA team
Zhang, Q.; Ding, H.; Yan, J.; Wang, W.; Ma, A.; Zhu, Z.; Cianflone, K.; Hu, F.B.; Hui, R.; Wang, D.W.
Plasma tissue kallikrein level is negatively associated with incident and recurrent stroke: a multicenter case-control study in China
Ann. Neurol.
70
265-273
2011
Homo sapiens
Manually annotated by BRENDA team
Nakamura, S.; Morimoto, N.; Tsuruma, K.; Izuta, H.; Yasuda, Y.; Kato, N.; Ikeda, T.; Shimazawa, M.; Hara, H.
Tissue kallikrein inhibits retinal neovascularization via the cleavage of vascular endothelial growth factor-165
Arterioscler. Thromb. Vasc. Biol.
31
1041-1048
2011
Homo sapiens
Manually annotated by BRENDA team
Dominek, P.; Campagnolo, P.; H-Zadeh, M.; Kraenkel, N.; Chilosi, M.; Sharman, J.A.; Caporali, A.; Mangialardi, G.; Spinetti, G.; Emanueli, C.; Pignatelli, M.; Madeddu, P.
Role of human tissue kallikrein in gastrointestinal stromal tumour invasion
Br. J. Cancer
103
1422-1431
2010
Homo sapiens
Manually annotated by BRENDA team
Spinetti, G.; Fortunato, O.; Cordella, D.; Portararo, P.; Kraenkel, N.; Katare, R.; Sala-Newby, G.B.; Richer, C.; Vincent, M.P.; Alhenc-Gelas, F.; Tonolo, G.; Cherchi, S.; Emanueli, C.; Madeddu, P.
Tissue kallikrein is essential for invasive capacity of circulating proangiogenic cells
Circ. Res.
108
284-293
2011
Homo sapiens
Manually annotated by BRENDA team
Wang, Y.D.; Lu, R.Y.; Huang, X.X.; Yuan, F.; Hu, T.; Peng, Y.; Huang, S.Q.
Human tissue kallikrein promoted activation of the ipsilesional sensorimotor cortex after acute cerebral infarction
Eur. Neurol.
65
208-214
2011
Homo sapiens
Manually annotated by BRENDA team
Lu, Z.; Yang, Q.; Cui, M.; Liu, Y.; Wang, T.; Zhao, H.; Dong, Q.
Tissue kallikrein induces SH-SY5Y cell proliferation via epidermal growth factor receptor and extracellular signal-regulated kinase1/2 pathway
Biochem. Biophys. Res. Commun.
446
25-29
2014
Homo sapiens
Manually annotated by BRENDA team
Oliveira, J.R.; Bertolin, T.C.; Andrade, D.; Oliveira, L.C.; Kondo, M.Y.; Santos, J.A.; Blaber, M.; Juliano, L.; Severino, B.; Caliendo, G.; Santagada, V.; Juliano, M.A.
Specificity studies on Kallikrein-related peptidase 7 (KLK7) and effects of osmolytes and glycosaminoglycans on its peptidase activity
Biochim. Biophys. Acta
1854
73-83
2015
Homo sapiens
Manually annotated by BRENDA team
Su, J.; Tang, Y.; Zhou, H.; Liu, L.; Dong, Q.
Tissue kallikrein protects neurons from hypoxia/reoxygenation-induced cell injury through Homer1b/c
Cell. Signal.
24
2205-2215
2012
Homo sapiens
Manually annotated by BRENDA team
Yao, Y.Y.; Fu, C.; Ma, G.S.; Feng, Y.; Shen, C.X.; Wu, G.Q.; Zhang, X.G.; Ding, J.D.; Tang, C.C.; Chen, Z.; Dai, Q.M.; Tong, J.Y.; Luo, D.; Zhu, J.; Zhi, H.; Li, Y.J.; Ju, C.W.; Lu, J.; Chao, J.; Chao, L.
Tissue kallikrein is related to the severity of coronary artery disease
Clin. Chim. Acta
423
90-98
2013
Homo sapiens
Manually annotated by BRENDA team
Yao, Y.; Sheng, Z.; Li, Y.; Yan, F.; Fu, C.; Li, Y.; Ma, G.; Liu, N.; Chao, J.; Chao, L.
Tissue kallikrein promotes cardiac neovascularization by enhancing endothelial progenitor cell functional capacity
Hum. Gene Ther.
23
859-870
2012
Homo sapiens
Manually annotated by BRENDA team
Koumandou, V.L.; Scorilas, A.
Evolution of the plasma and tissue kallikreins, and their alternative splicing isoforms
PLoS ONE
8
e68074
2013
Homo sapiens
Manually annotated by BRENDA team
Yiu, W.H.; Wong, D.W.; Chan, L.Y.; Leung, J.C.; Chan, K.W.; Lan, H.Y.; Lai, K.N.; Tang, S.C.
Tissue kallikrein mediates pro-inflammatory pathways and activation of protease-activated receptor-4 in proximal tubular epithelial cells
PLoS One
9
e88894
2014
Homo sapiens
Manually annotated by BRENDA team
Pathak, M.; Wong, S.S.; Dreveny, I.; Emsley, J.
Structure of plasma and tissue kallikreins
Thromb. Haemost.
110
423-433
2013
Homo sapiens, Homo sapiens (P06870)
Manually annotated by BRENDA team
Petraki, C.; Youssef, Y.M.; Dubinski, W.; Lichner, Z.; Scorilas, A.; Pasic, M.D.; Komborozos, V.; Khalil, B.; Streutker, C.; Diamandis, E.P.; Yousef, G.M.
Evaluation and prognostic significance of human tissue kallikrein-related peptidase 10 (KLK10) in colorectal cancer
Tumour Biol.
33
1209-1214
2012
Homo sapiens
Manually annotated by BRENDA team
Figueroa, C.D.; Molina, L.; Bhoola, K.D.; Ehrenfeld, P.
Overview of tissue kallikrein and kallikrein-related peptidases in breast cancer
Biol. Chem.
399
937-957
2018
Homo sapiens
Manually annotated by BRENDA team
Santos, J.A.; Kondo, M.Y.; Freitas, R.F.; dos Santos, M.H.; Ramalho, T.C.; Assis, D.M.; Juliano, L.; Juliano, M.A.; Puzer, L.
The natural flavone fukugetin as a mixed-type inhibitor for human tissue kallikreins
Bioorg. Med. Chem. Lett.
26
1485-1489
2016
Homo sapiens
Manually annotated by BRENDA team
Filippou, P.S.; Ren, A.H.; Bala, S.; Papaioannou, M.D.; Brinc, D.; Prassas, I.; Karakosta, T.; Diamandis, E.P.
Biochemical characterization of human tissue kallikrein 15 and examination of its potential role in cancer
Clin. Biochem.
58
108-115
2018
Homo sapiens (Q9H2R5), Homo sapiens
Manually annotated by BRENDA team
Liu, Y.; Lu, Z.; Cui, M.; Yang, Q.; Tang, Y.; Dong, Q.
Tissue kallikrein protects SH-SY5Y neuronal cells against oxygen and glucose deprivation-induced injury through bradykinin B2 receptor-dependent regulation of autophagy induction
J. Neurochem.
139
208-220
2016
Homo sapiens
Manually annotated by BRENDA team
Chen, J.; Wu, J.; Wu, Q.; Hu, Z.
Biosynthesis and secretion of human tissue kallikrein in transgenic Chlamydomonas reinhardtii
Mar. Drugs
16
E493
2018
Homo sapiens
Manually annotated by BRENDA team
Yunchang, M.; Qinxue, D.; Binbin, J.; Xin, H.; Lili, Y.; Linbi, C.; Wujun, G.; Pengbo, Z.; Junlu, W.
Human tissue kallikrein ameliorates cerebral vasospasm in a rabbit model of subarachnoid hemorrhage
Neurol. Res.
37
1082-1089
2015
Homo sapiens
Manually annotated by BRENDA team