Information on EC 3.4.21.35 - tissue kallikrein

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The expected taxonomic range for this enzyme is: Euteleostomi

EC NUMBER
COMMENTARY hide
3.4.21.35
-
RECOMMENDED NAME
GeneOntology No.
tissue kallikrein
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage of Arg-/- bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-/- or Leu-/-. The rat enzyme is unusual in liberating bradykinin directly from autologous kininogens by cleavage at two Arg-/- bonds
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
CAS REGISTRY NUMBER
COMMENTARY hide
389069-73-2
-
9001-01-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
kallikrein A and B
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-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
-
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-
Manually annotated by BRENDA team
gene KLK1, glandular kallikrein 1 precursor
SwissProt
Manually annotated by BRENDA team
african soft-furred rat; gene KLK1, renal glanduar kallikrein precursor
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene KLK1, glandular kallikrein 1 precursor
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-MISLMKRPPGFSPFRSS(PO3H2)RI-NH2 + H2O
2-aminobenzoyl-MISLM + KRPPGFSPFRSS(PO3H2)RI-NH2
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-MISLMKRPPGFSPFRSSRI-NH2 + H2O
2-aminobenzoyl-MISLM + KRPPGFSPFRSSRI-NH2
show the reaction diagram
-
-
-
-
?
Abz-ALFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-FLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-FRSSR-Q-EDDnp + H2O
Abz-FR + SSR-Q-EDDnp
show the reaction diagram
-
-
-
?
Abz-GFSPFRSLRI-Q-EDDnp + H2O
Abz-GFSPFR + SLRI-Q-EDDnp
show the reaction diagram
-
-
-
?
Abz-GFSPFRSSRI-Q-EDDnp + H2O
Abz-GFSPFR + SSRI-Q-EDDnp
show the reaction diagram
-
-
-
?
Abz-GLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-HLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-ILFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KAFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KFFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFFSK-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFRSK-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSAKQ-EDDnp + H2O
?
show the reaction diagram
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-
-
-
?
Abz-KLFSGKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSIKQ-EDDnp + H2O
?
show the reaction diagram
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-
-
-
?
Abz-KLFSLKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSNKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSRKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSSDQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSSEQ-EDDnp + H2O
Abz-KLFSS + L-Glu-L-Gln-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLFSSGQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSSIQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSSK-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSSLQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSSNQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSSQQ-EDDnp + H2O
?
show the reaction diagram
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-
-
-
?
Abz-KLFSSRQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSSSQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSSVQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFSVKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFTSK-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KLFWSK-EDDnp + H2O
?
show the reaction diagram
-
-
-
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?
Abz-KLYSSK-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KPFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KVFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-KYFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-LLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-MISLMKLP-Q-EDDnp + H2O
Abz-MISLM + KLP-Q-EDDnp
show the reaction diagram
-
-
-
?
Abz-MISLMKRP-Q-EDDnp + H2O
Abz-MISLM + KRP-Q-EDDnp
show the reaction diagram
-
-
-
?
Abz-MISLMKRPPGFSPFRSSRI-NH2 + H2O
Abz-MISLM + KRPPGFSPF + RSSRI-NH2
show the reaction diagram
-
-
-
-
?
Abz-NLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-NLYRVE-Q-EDDnp + H2O
Abz-NLY + RVE-Q-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-QLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-RLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-VLFSSKQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
Ac-GKAFRR-L12ADT + H2O
?
show the reaction diagram
-
peptide coupled to cytotoxin thapsigarin, L12ADT, as an enzyme-activated prodrug. Enzyme cleaves at R-residue adjacent to L12ADT
-
-
?
acetyl-Ala-Arg methyl ester + H2O
acetyl-Ala-Arg + methanol
show the reaction diagram
-
-
-
-
?
acetyl-Ala-Arg-Arg methyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
acetyl-Ala-Leu-Arg-p-nitroanilide + H2O
acetyl-Ala-Leu-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-Ala-Phe-Arg methyl ester + H2O
acetyl-Ala-Phe-Arg + methanol
show the reaction diagram
-
-
-
-
?
acetyl-Ala-Phe-Arg-Ala-NH2 + H2O
acetyl-Ala-Phe-Arg + Ala-NH2
show the reaction diagram
-
-
-
-
?
acetyl-Ala-Phe-Arg-p-nitroanilide + H2O
acetyl-Ala-Phe-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-Ala-Thr-Arg-p-nitroanilide + H2O
acetyl-Ala-Thr-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-Arg methyl ester + H2O
acetyl-Arg + methanol
show the reaction diagram
-
-
-
-
?
acetyl-Arg-Ala-NH2 + H2O
acetyl-Arg + Ala-NH2
show the reaction diagram
-
-
-
-
?
acetyl-Arg-p-nitroanilide + H2O
acetyl-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-D-Ala-Arg methyl ester + H2O
acetyl-D-Ala-Arg + methanol
show the reaction diagram
-
-
-
-
?
acetyl-D-Phe-Arg methyl ester + H2O
acetyl-D-Phe-Arg + methanol
show the reaction diagram
-
-
-
-
?
acetyl-Glu-Leu-Arg-p-nitroanilide + H2O
acetyl-Glu-Leu-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-Glu-Lys-Arg-p-nitroanilide + H2O
acetyl-Glu-Lys-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-Glu-Phe-Arg-p-nitroanilide + H2O
acetyl-Glu-Phe-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-Glu-Thr-Arg-p-nitroanilide + H2O
acetyl-Glu-Thr-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-Gly-Arg methyl ester + H2O
acetyl-Gly-Arg + methanol
show the reaction diagram
-
-
-
-
?
acetyl-Gly-Arg-Ser-Val-Gln + H2O
acetyl-Gly-Arg + Ser-Val-Gln
show the reaction diagram
-
-
-
?
acetyl-Ile-Arg methyl ester + H2O
acetyl-Ile-Arg + methanol
show the reaction diagram
-
-
-
-
?
acetyl-Leu-Arg methyl ester + H2O
acetyl-Leu-Arg + methanol
show the reaction diagram
-
-
-
-
?
acetyl-Met-Arg methyl ester + H2O
acetyl-Met-Arg + methanol
show the reaction diagram
-
-
-
-
?
acetyl-Phe-Arg methyl ester + H2O
acetyl-Phe-Arg + methanol
show the reaction diagram
acetyl-Phe-Arg-p-nitroanilide + H2O
acetyl-Phe-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-Phe-Arg-Ser-NH2 + H2O
acetyl-Phe-Arg + Ser-NH2
show the reaction diagram
-
-
-
?
acetyl-Phe-Arg-Ser-Val-Gln + H2O
acetyl-Phe-Arg + Ser-Val-Gln
show the reaction diagram
-
-
-
?
acetyl-Phe-Arg-Ser-Val-NH2 + H2O
acetyl-Phe-Arg + Ser-Val-NH2
show the reaction diagram
-
-
-
?
acetyl-Pro-Arg methyl ester + H2O
acetyl-Pro-Arg + methanol
show the reaction diagram
-
-
-
-
?
acetyl-Pro-Phe-Arg methyl ester + H2O
acetyl-Pro-Phe-Arg + methanol
show the reaction diagram
-
-
-
-
?
acetyl-Pro-Phe-Arg-Ala-NH2 + H2O
acetyl-Pro-Phe-Arg + Ala-NH2
show the reaction diagram
-
-
-
-
?
acetyl-Pro-Phe-Arg-p-nitroanilide + H2O
acetyl-Pro-Phe-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-Trp-2-aminohexanoic acid methyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
acetyl-Tyr-Arg methyl ester + H2O
acetyl-Tyr-Arg + methanol
show the reaction diagram
-
-
-
-
?
acetyl-Val-Arg methyl ester + H2O
acetyl-Val-Arg + methanol
show the reaction diagram
-
-
-
-
?
Ala-Ala-Arg-7-amido-4-methylcoumarin + H2O
Ala-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Angiotensin I + H2O
?
show the reaction diagram
-
reaction with prostate enzyme, very low reaction with submandibular enzyme
-
-
?
angiotensinogen + H2O
?
show the reaction diagram
-
reaction with prostate enzyme, no reaction with submandibular enzyme
-
-
?
angiotensinogen + H2O
angiotensin
show the reaction diagram
-
-
-
?
benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzoyl-Pro-Phe-Arg-p-nitroanilide + H2O
benzoyl-Pro-Phe-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Arg methyl ester + H2O
benzyloxycarbonyl-Arg + methanol
show the reaction diagram
benzyloxycarbonyl-Arg-Ala-NH2 + H2O
benzyloxycarbonyl-Arg + Ala-NH2
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Arg-p-nitroanilide + H2O
benzyloxycarbonyl-Arg + p-nitroaniline
show the reaction diagram
Benzyloxycarbonyl-Lys methyl ester + H2O
Benzyloxycarbonyl-Lys + methanol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
about 30% of the activity with Pro-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Pro-Gly-Arg methyl ester + H2O
benzyloxycarbonyl-Pro-Gly-Arg + methanol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Pro-Phe-Arg methyl ester + H2O
benzyloxycarbonyl-Pro-Phe-Arg + methanol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Tyr-4-nitrophenyl ester + H2O
benzyloxycarbonyl-Tyr + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
citrated dog plasma + H2O
?
show the reaction diagram
-
-
-
-
?
D-Ile-Pro-Arg-4-nitroanilide + H2O
D-Ile-Pro-Arg + 4-nitroaniline
show the reaction diagram
-
weak activity
-
-
?
D-Pro-Phe-Arg-4-methylcoumarin-7-amide + H2O
?
show the reaction diagram
D-Pro-Phe-Arg-4-nitroanilide + H2O
D-Pro-Phe-Arg + 4-nitroaniline
show the reaction diagram
-
weak activity
-
-
?
D-Pro-Phe-Phe-4-methylcoumarin-7-amide + H2O
?
show the reaction diagram
D-Pro-Phe-Phe-7-amido4-methylcoumarin + H2O
D-Pro-Phe-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
-
D-Val-Leu-Arg-4-nitroanilide + H2O
D-Val-Leu-Arg + 4-nitroaniline
show the reaction diagram
D-Val-Leu-Arg-7-amido-4-trifluoromethylcoumarin + H2O
D-Val-Leu-Arg + 7-amino-4-trifluoromethylcoumarin
show the reaction diagram
-
-
-
-
?
D-Val-Leu-Arg-p-nitroanilide + H2O
D-Val-Leu-Arg + p-nitroaniline
show the reaction diagram
D-Val-Leu-Lys-4-nitroanilide + H2O
D-Val-Leu-Lys + 4-nitroaniline
show the reaction diagram
DL-Val-Leu-Arg-p-nitroanilide + H2O
DL-Val-Leu-Arg + p-nitroaniline
show the reaction diagram
epithelial sodium channel + H2O
activated epithelial sodium channel + ?
show the reaction diagram
-
-
-
-
?
Fibronectin + H2O
?
show the reaction diagram
FSPFRSVQ + H2O
FSPFR + SVQ
show the reaction diagram
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
Gln-Ala-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
H-D-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
H-D-Pro-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
i.e. S-2302
-
-
?
insulin-like growth factor binding protein + H2O
?
show the reaction diagram
-
-
-
-
?
insulin-like growth factor binding proteins
?
show the reaction diagram
insulin-like growth factor binding proteins
insulin-like growth factor binding protein fragments
show the reaction diagram
kinin B2 receptor + H2O
?
show the reaction diagram
-
tissue kallikrein directly activates kinin B2 receptor in the absence of kininogen
-
-
?
kininogen + H2O
bradykinin + ?
show the reaction diagram
-
-
-
-
?
kininogen + H2O
bradykinin + kinin
show the reaction diagram
kininogen + H2O
bradykinin + kinin heavy chain + kinin light chain
show the reaction diagram
kininogen + H2O
kallidin + kinin heavy chain + kinin light chain
show the reaction diagram
kininogen + H2O
kinin + ?
show the reaction diagram
kininogen + H2O
kinin + bradykinin
show the reaction diagram
-
-
-
-
?
L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
L-Pro-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Laminin + H2O
?
show the reaction diagram
-
-
-
-
?
Leu-Leu-Arg-7-amido-4-methylcoumarin + H2O
Leu-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
low density lipoprotein + H2O
?
show the reaction diagram
-
-
-
?
low molecular weight kininogen + H2O
kallidin + kinin
show the reaction diagram
-
-
-
-
?
low-molecular-weight kininogen + H2O
GMISLM + KRPPGFSPFR + SSRIG
show the reaction diagram
-
KRPPGFSPFR is kallidin
-
?
low-molecular-weight kininogen + H2O
kallidin + kinin
show the reaction diagram
N-alpha-acetyl-Phe-Arg ethyl ester + H2O
N-alpha-acetyl-Phe-Arg + ethanol
show the reaction diagram
-
-
-
-
?
N-alpha-acetyl-Tyr ethyl ester + H2O
N-alpha-acetyl-Tyr + ethanol
show the reaction diagram
N-alpha-benzoyl-L-Arg ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
show the reaction diagram
N-alpha-benzoyl-L-Arg methyl ester + H2O
N-alpha-benzoyl-L-Arg + methanol
show the reaction diagram
N-alpha-benzoyl-L-citrulline methyl ester + H2O
N-alpha-benzoyl-L-citrulline + methanol
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Lys methyl ester + H2O
N-alpha-benzoyl-L-Lys + methanol
show the reaction diagram
N-alpha-benzoyl-L-Orn methyl ester + H2O
N-alpha-benzoyl-L-Orn + methanol
show the reaction diagram
-
-
-
-
?
N-alpha-tosyl-L-arginine methyl ester + H2O
N-alpha-tosyl-L-arginine + methanol
show the reaction diagram
-
-
-
-
?
N-methoxysuccinyl-Arg-Pro-Tyr-4-nitroanilide + H2O
N-methoxysuccinyl-Arg-Pro-Tyr + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AIKFFIRE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + IRE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFQRE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + QRE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFSAE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + SAE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFSKE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + SKE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFSPE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + SPE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFSRE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + SRE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFSRQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + SRQ-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
substrate sequence is derived from the kallistatin reactive centre loop sequence
-
?
o-aminobenzoyl-AIKFFSSE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + SSE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIKFFTRE-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-AIKFF + TRE-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
-
-
?
o-aminobenzoyl-FRAPR-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
o-aminobenzoyl-FRLVR-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
o-aminobenzoyl-FRSSR-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-FR + SSR-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
o-aminobenzoyl-FRSVQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-FR + SVQ-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
o-aminobenzoyl-GFSPF-4-(aminocyclohexyl)alanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-GFSPF-4-(aminomethyl)-N-isopropylphenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-GFSPF-4-(aminomethyl)phenylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-GFSPF-4-piperidinylalanine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-GFSPF-Nim-(dimethyl)histidine-SSRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-GFSPFRAPRVQ-ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-GFSPFR + APRVQ-ethylenediamine 2,4-dinitrophenol + o-aminobenzoyl-GFSPF + RAPRVQ-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
o-aminobenzoyl-GFSPFRSSRIGEIKEEQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-GFSPFR + SSRIGEIKEEQ-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
o-aminobenzoyl-GFSPFRSSRQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-GFSPFR + SSRQ-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
o-aminobenzoyl-GFSPFRSVQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-GFSPFR + SVQ-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
o-aminobenzoyl-GFSPFRSVQVMKYEGSQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-GFSPFR + SVQVMKYEGSQ-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
o-aminobenzoyl-GFSPFRSVTVQ-ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-GFSPFR + SVTVQ-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
o-aminobenzoyl-LMKRP-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-LM + KRP-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
o-aminobenzoyl-LPRTMFIQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
shortened, quenched fluorescent substrate, synthesized based on Bauhinia ungulata factor Xa inhibitor reactive sequence site
-
-
?
o-aminobenzoyl-LPRTMQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
shortened, quenched fluorescent substrate, synthesized based on Bauhinia ungulata factor Xa inhibitor reactive sequence site
-
-
?
o-aminobenzoyl-MTEMARRPPGFSPFRSVTVQ-NH2 + H2O
o-aminobenzoyl-MTEMAR + RPPGFSPFR + SVTVQ-NH2
show the reaction diagram
o-aminobenzoyl-MTEMARRPQ-ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-MTEMAR + RPQ-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
o-aminobenzoyl-MTSVIRRPPGFSPFRAPRV-NH2 + H2O
des[Arg9] bradykinin + ?
show the reaction diagram
-
preferential hydrolysis of Phe-Arg compared with Arg-Ala bond, preferential release of des[Arg9] bradykinin
-
-
?
o-aminobenzoyl-MTSVIRRPPGFSPFRAPRV-NH2 + H2O
o-aminobenzoyl-MTSVIR + RPPGFSPFR + APRV-NH2
show the reaction diagram
o-aminobenzoyl-MTSVIRRPQ-ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-MTSVIR + RPQ-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
o-aminobenzoyl-Phe-4-(aminomethyl)phenylalanine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-Phe-4-(aminomethyl)phenylalanine-Ser-Arg + Gln-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-Phe-Arg-Arg--ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-Phe-Arg + Arg-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
-
slow hydrolysis
-
-
?
o-aminobenzoyl-Phe-Arg-Arg-ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-Phe-Arg + Arg-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
o-aminobenzoyl-Phe-Arg-Lys-GFSPFRSVTVQ-ethylenediamine 2,4-dinitrophenol + H2O
o-aminobenzoyl-Phe-Arg + Lys-ethylenediamine 2,4-dinitrophenol
show the reaction diagram
o-aminobenzoyl-Phe-Nim-(dimethyl)histidine-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-Phe-Nim-(dimethyl)histidine-Ser-Arg + Gln-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
-
synthetic internally quenched fluorescent peptides with substitution for a non-natural amino acid, sequence is based on the human kininogen sequence at the C-terminal region of bradykinin
-
-
?
o-aminobenzoyl-QPLGMISLMKRP-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-QPLGMISLM + KRP-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
o-aminobenzoyl-QPLGQTSLMKRP-N-(2,4-dinitrophenyl)ethylene diamine + H2O
o-aminobenzoyl-QPLGQTSLM + KRP-N-(2,4-dinitrophenyl)ethylene diamine
show the reaction diagram
o-aminobenzoyl-SVIRRVQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
o-aminobenzoyl-TSVIRRPQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-VMIAALPRTMFIQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
o-aminobenzoyl-VMIAALPRTMQ-N-(2,4-dinitrophenyl)ethylene diamine + H2O
?
show the reaction diagram
PFRSVQ + H2O
PFR + SVQ
show the reaction diagram
-
-
-
?
Phe-Arg-Ser-Val-Gln + H2O
Phe-Arg + Ser-Val-Gln
show the reaction diagram
-
-
-
?
Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
Phe-Ser-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
poly-L-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
polypeptide + H2O
peptides
show the reaction diagram
precursor of atrial natriuretic factor + H2O
natriuretic factor
show the reaction diagram
-
-
-
?
pro-collagenase + H2O
collagease
show the reaction diagram
-
-
-
?
pro-insulin + H2O
insulin + fragment
show the reaction diagram
-
-
-
?
pro-interleukin-1beta + H2O
interleukin-1beta + ?
show the reaction diagram
-
reaction catalyzed by mK13
-
-
?
Pro-Phe-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
Pro-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
pro-renin + H2O
renin
show the reaction diagram
-
-
-
?
pro-urokinase + H2O
urokinase
show the reaction diagram
-
-
-
-
?
protein C inhibitor + H2O
?
show the reaction diagram
-
-
-
-
?
RPPGFSPFRSVQ + H2O
RPPGFSPFR + SVQ
show the reaction diagram
-
-
-
?
S-2266 + H2O
?
show the reaction diagram
salmine + H2O
?
show the reaction diagram
-
-
-
-
?
semenogelin I + H2O
KGISSQY + SNTE
show the reaction diagram
-
-
-
?
semenogelin I + H2O
semenogelin I fragments
show the reaction diagram
-
-
-
-
?
semenogelin II + H2O
semenogelin II fragments
show the reaction diagram
-
-
-
-
?
single-chain tissue-type plasminogen activator + H2O
?
show the reaction diagram
somatostatin + H2O
?
show the reaction diagram
-
-
-
-
?
Substance P + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-Val-Pro-Phe-thiobenzyl ester + H2O
succinyl-Val-Pro-Phe + phenylmethanethiol
show the reaction diagram
-
-
-
-
?
tert-butyloxycarbonyl-Gln-Ala-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
about 15% of the activity with Pro-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
about 50% of the activity with Pro-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
about 20% of the activity with Pro-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
Tosyl-Arg methyl ester + H2O
Tosyl-Arg + methanol
show the reaction diagram
tosyl-Gly-Pro-Arg-p-nitroanilide + H2O
tosyl-Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Val-Leu-Arg-4-nitroanilide + H2O
Val-Leu-Arg + 4-nitroaniline
show the reaction diagram
-
tracheobronchial epithelial cells grown at the air/liquid interface
-
-
?
Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
Val-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
vascular endothelial growth factor 164 + H2O
?
show the reaction diagram
-
-
-
-
?
vascular endothelial growth factor-165 + H2O
?
show the reaction diagram
-
-
-
-
?
Vasoactive intestinal peptide + H2O
?
show the reaction diagram
-
-
-
?
WDDDDNLLVCDVPIR + H2O
WDDDDNL + LVCDVPIR
show the reaction diagram
-
-
-
-
?
Z-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
zymogen of prostate-specific antigen + H2O
prostate-specific antigen + APLILSR
show the reaction diagram
-
-
-
-
?
zymogen of urokinase-type plasminogen activator + H2O
urokinase-type plasminogen activator
show the reaction diagram
-
activation of inactive zymogen to mature active enzyme
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
epithelial sodium channel + H2O
activated epithelial sodium channel + ?
show the reaction diagram
-
-
-
-
?
insulin-like growth factor binding proteins
?
show the reaction diagram
kininogen + H2O
bradykinin + ?
show the reaction diagram
-
-
-
-
?
kininogen + H2O
bradykinin + kinin heavy chain + kinin light chain
show the reaction diagram
-
important role in bradykinin release in seminal fluid
-
?
kininogen + H2O
kallidin + kinin heavy chain + kinin light chain
show the reaction diagram
kininogen + H2O
kinin + ?
show the reaction diagram
-
-
-
-
?
kininogen + H2O
kinin + bradykinin
show the reaction diagram
-
-
-
-
?
low-molecular-weight kininogen + H2O
GMISLM + KRPPGFSPFR + SSRIG
show the reaction diagram
P06870
-
KRPPGFSPFR is kallidin
-
?
low-molecular-weight kininogen + H2O
kallidin + kinin
show the reaction diagram
polypeptide + H2O
peptides
show the reaction diagram
semenogelin I + H2O
KGISSQY + SNTE
show the reaction diagram
P06870
-
-
-
?
single-chain tissue-type plasminogen activator + H2O
?
show the reaction diagram
-
glandular kallikrein 24 may play a role in degradation of extracellular matrix proteins in the interstitial area surrounding the Leydig cells of the adult mouse testis, due not only to its own activity, but also to that of plasmin produced by the single-chain tissue-type plasminogen activator-converting activity of glandular kallikrein 24
-
-
?
vascular endothelial growth factor 164 + H2O
?
show the reaction diagram
-
-
-
-
?
vascular endothelial growth factor-165 + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
Na+, K+, Li+, Rb+ or Ca2+ required for activation
K+
-
Na+, K+, Li+, Rb+ or Ca2+ required for activation
Li+
-
Na+, K+, Li+, Rb+ or Ca2+ required for activation
Rb+
-
Na+, K+, Li+, Rb+ or Ca2+ required for activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(4-amidino-phenyl)-methane-sulfonyl fluoride
2.5 mM, complete inhibition
(tris[2-carboxyethyl] phosphine) and dihydro-lipoic acid
-
13% residual activity at 3 mM
4-(2-aminoethyl)benzenesulfonyl fluoride
-
is an active-site-directed covalent inhibitor
Abz-KDFSSKQ-EDDnp
-
-
-
Abz-KEFSSKQ-EDDnp
-
-
-
Abz-KGFSSKQ-EDDnp
-
-
-
Abz-KHFSSKQ-EDDnp
-
-
-
Abz-KIFSSKQ-EDDnp
-
-
-
Abz-KKFSSKQ-EDDnp
-
-
-
Abz-KLASSK-EDDnp
-
-
-
Abz-KLDSSK-EDDnp
-
-
-
Abz-KLESSK-EDDnp
-
-
-
Abz-KLFESK-EDDnp
-
-
-
Abz-KLFHSK-EDDnp
-
-
-
Abz-KLFISK-EDDnp
-
-
-
Abz-KLFKSK-EDDnp
-
-
-
Abz-KLFLSK-EDDnp
-
-
-
Abz-KLFNSK-EDDnp
-
-
-
Abz-KLFPSK-EDDnp
-
-
-
Abz-KLFSEKQ-EDDnp
-
-
-
Abz-KLFSFKQ-EDDnp
-
-
-
Abz-KLFSHKQ-EDDnp
-
-
-
Abz-KLFSPKQ-EDDnp
-
-
-
Abz-KLFSQKQ-EDDnp
-
-
-
Abz-KLFVSK-EDDnp
-
-
-
Abz-KLGSSK-EDDnp
-
-
-
Abz-KLHSSK-EDDnp
-
-
-
Abz-KLISSK-EDDnp
-
-
-
Abz-KLKSSK-EDDnp
-
-
-
Abz-KLLSSK-EDDnp
-
-
-
Abz-KLMSSK-EDDnp
-
-
-
Abz-KLNSSK-EDDnp
-
-
-
Abz-KLPSSK-EDDnp
-
-
-
Abz-KLQSSK-EDDnp
-
-
-
Abz-KLRSSK-EDDnp
-
-
-
Abz-KLSSSK-EDDnp
-
-
-
Abz-KLTSSK-EDDnp
-
-
-
Abz-KLVSSK-EDDnp
-
-
-
Abz-KLWSSK-EDDnp
-
-
-
Abz-KMFSSKQ-EDDnp
-
-
-
Abz-KNFSSKQ-EDDnp
-
-
-
Abz-KQFSSKQ-EDDnp
-
-
-
Abz-KRFSSKQ-EDDnp
-
-
-
Abz-KSFSSKQ-EDDnp
-
-
-
Abz-KTFSSKQ-EDDnp
-
-
-
Abz-KWFSSKQ-EDDnp
-
-
-
acetyl-D-Lys-L-Phe-L-Arg-D-Arg-L-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
-
acetyl-D-Lys-L-Phe-L-Arg-D-Arg-L-Gln-NH2
-
-
acetyl-D-Lys-L-Phe-L-Arg-D-Arg-NH2
-
-
acetyl-D-Lys-L-Phe-L-Arg-L-Leu-L-Glu-NH2
-
-
acetyl-Lys-Phe-Phe-Pro-Leu-Glu-NH2
-
leader compound for design of specific inhibitors of K1; weak inhibition
Al3+
-
linear mixed inhibition
Alpha1-antitrypsin
amiloride
-
-
antipain
Aprotinin
Bauhinia bauhinioides trypsin inhibitor II
-
i.e. BbTI-II; N-terminal sequence of the protein is SVVVDTNGQPVSNGADAYYLV
-
benzamidine
beta-mercaptoethanol
-
65% residual activity at 15 mM
Ca2+
-
linear competitive
Cd2+
-
-
chymostatin
cinnamoyl imidazole
-
-
Co2+
-
-
convallatoxin
-
-
Cu2+
-
-
D-Pro-Phe-Arg-chloromethane
-
is an active-site-directed covalent inhibitor
digitoxigenin
-
-
digitoxin
-
-
diglutamic acid
-
-
-
digoxin
-
-
dihydrolipoate
-
35% residual activity at 3 mM
diisopropyl fluorophosphate
diisopropylfluorophosphate
-
2.0 mM, complete inhibition of hydrolysis of Pro-Phe-Arg-4-methylcoumarin-7-amide
dithiothreitol
-
complete inhibition at 3 mM
DX-2300
EGFR inhibitor
-
blocks tissue kallikrein's skin wound healing effect
-
Egg white
-
-
-
epidermal growth factor receptor inhibitor
-
-
-
glutathione
-
16% residual activity at 15 mM
Hg2+
-
-
hK11-specific monoclonal antibody
up to 40% inhibition
-
Human plasma
-
-
-
human urine
-
-
-
hyaluronic acid
-
binds to the enzyme in vivo
icatibant
indolacryloyl imidazole
-
-
-
K+
-
linear competitive
kallikrein antibody
kallistatin
Leupeptin
Mn2+
-
-
Nalpha-p-tosyl-L-lysine chloromethyl ketone
-
0.1 mM, 10% inhibition of hydrolysis of Pro-Phe-Arg-4-methylcoumarin-7-amide
Nalpha-p-tosyl-L-phenylalanine chloromethyl ketone
-
0.1 mM, 5% inhibition of hydrolysis of Pro-Phe-Arg-4-methylcoumarin-7-amide
Ni2+
-
-
o-aminobenzoyl-AIKFFARE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFERE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFKRE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFLRE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFNRE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFPRE-N-(2,4-dinitrophenyl)ethylene diamine
-
-
o-aminobenzoyl-AIKFFRRE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSEE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSIE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSLE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSNE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSQE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSTE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFSVE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-AIKFFVRE-N-(2,4-dinitrophenyl)ethylene diamine
-
compound gets poorly hydrolyzed as substrate and is a competitive inhibitor
o-aminobenzoyl-D-Lys-L-Phe-L-Arg-D-Arg-L-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
-
o-aminobenzoyl-D-Lys-L-Phe-L-Arg-L-Pro-L-Arg-L-Gln N-(2,4-dinitrophenyl)ethylene diamine
-
-
o-aminobenzoyl-D-Lys-L-Phe-L-Phe-D-Arg-L-Gln-N-(2,4-dinitrophenyl)ethylene diamine
-
-
o-aminobenzoyl-D-Lys-L-Phe-L-Phe-L-Pro-L-Arg-L-Gln N-(2,4-dinitrophenyl)ethylene diamine
-
-
o-aminobenzoyl-D-Lys-L-Phe-L-Phe-L-Pro-L-Leu-L-Glu N-(2,4-dinitrophenyl)ethylene diamine
-
-
o-aminobenzoyl-F-4-(aminomethyl)cyclohexylalanine-SRQ-N-(2,4-dinitrophenyl)ethylene diamine
-
K1, highly specific
o-aminobenzoyl-TSVIRRPQ-ethylenediamine
-
-
Ouabain
-
-
p-nitrophenyl p'-guanidinobenzoate
-
-
pancratic inhibitor
-
-
-
Pentamidine
-
-
Pepstatin
-
0.01 mM, 10% inhibition of hydrolysis of Pro-Phe-Arg-4-methylcoumarin-7-amide
peruvoside
-
-
Phe-Phe-Arg-CH2Cl
-
-
phenylmethylsulfonyl fluoride
-
-
protease inhibitor-6
-
from prostate, increased expression in prostate carcinoma; intracellular serine protease inhibitor with both antitrypsin and antichymotrypsin activity; rapid complex formation with the enzyme, involved in neoplastic progression
-
Protein C inhibitor
recombinant alpha1-antichymotrypsin
-
generation of selective inhibitors based on substrates selected from a pentapeptide phage-display library and transplanted to the reactive-site loop of alpha1-antichymotrypsin. Engineered alpha1-antichymotrypsins with peptides RGR-SE and ER-VSP are inhibitory and specific to enzyme.
-
Soybean trypsin inhibitor
tissue kallikrein-binding protein
-
rapidly forms a covalent complex with tissue kallikrein
-
tosyl-L-Lys-chloromethylketone
-
-
Trasylol
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
aldosterone
-
increased aldosterone levels lead to enhanced kallikrein activity
bovine serum albumin
-
1 mg/ml, activates
-
Brij-58
-
0.1%, activates
chymase
-
stimulation, in correlation with secretory leukoprotease inhibitor stimulation, in stimulated serous cells
-
deoxycholate
-
0.1%, activates
hyaluronidase
-
increases K2 enzyme activity by digesting the K2-bound hyaluronic acid
-
Lima-bean trypsin inhibitor
Lubrol Px
-
0.1%, activates
ovomucoid trypsin inhibitor
Soybean trypsin inhibitor
-
at low concentrations
-
Thermolysin
-
thermolysin activates prokallikrein cleaving off the N-terminal Ile1-Val2
-
Triton X-100
-
0.1%, activates
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0043
Abz-ALFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0032
Abz-FLFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0015
Abz-FRSSR-Q-EDDnp
pH and temperature not specified in the publication
-
0.00036
Abz-GFSPFRSLRI-Q-EDDnp
pH and temperature not specified in the publication
-
0.0002
Abz-GFSPFRSSRI-Q-EDDnp
pH and temperature not specified in the publication
0.0062
Abz-GLFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0034
Abz-HLFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0027
Abz-ILFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0035
Abz-KAFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.003
Abz-KFFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0016
Abz-KLFFSK-EDDnp
-
at pH 7.5 and 37C
-
0.0006
Abz-KLFRSK-EDDnp
-
at pH 7.5 and 37C
-
0.0062
Abz-KLFSAKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0027
Abz-KLFSGKQ-EDDnp, Abz-KLFSIKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0037
Abz-KLFSLKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0023
Abz-KLFSNKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0015
Abz-KLFSRKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0032
Abz-KLFSSDQ-EDDnp
-
at pH 7.5 and 37C
-
0.0127
Abz-KLFSSEQ-EDDnp
-
at pH 7.5 and 37C
-
0.0016
Abz-KLFSSGQ-EDDnp
-
at pH 7.5 and 37C
-
0.0033
Abz-KLFSSIQ-EDDnp
-
at pH 7.5 and 37C
-
0.0018
Abz-KLFSSK-EDDnp, Abz-KLFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0032
Abz-KLFSSLQ-EDDnp
-
at pH 7.5 and 37C
-
0.0045
Abz-KLFSSNQ-EDDnp
-
at pH 7.5 and 37C
-
0.0043
Abz-KLFSSQQ-EDDnp
-
at pH 7.5 and 37C
-
0.0016
Abz-KLFSSRQ-EDDnp
-
at pH 7.5 and 37C
-
0.0059
Abz-KLFSSSQ-EDDnp
-
at pH 7.5 and 37C
-
0.0104
Abz-KLFSSVQ-EDDnp
-
at pH 7.5 and 37C
-
0.0027
Abz-KLFSVKQ-EDDnp, Abz-KLFTSK-EDDnp
-
at pH 7.5 and 37C
-
0.0024
Abz-KLFWSK-EDDnp
-
at pH 7.5 and 37C
-
0.0034
Abz-KLYSSK-EDDnp
-
at pH 7.5 and 37C
-
0.0025
Abz-KPFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0019
Abz-KVFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0027
Abz-KYFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0046
Abz-LLFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.009
Abz-MISLMKLP-Q-EDDnp
pH and temperature not specified in the publication
-
0.0007
Abz-MISLMKRP-Q-EDDnp
pH and temperature not specified in the publication
0.0017
Abz-NLFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0052
Abz-QLFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0041
Abz-RLFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.0021
Abz-VLFSSKQ-EDDnp
-
at pH 7.5 and 37C
-
0.08
acetyl-2-aminohexanoic acid-Arg methyl ester
-
-
-
0.6 - 1.14
acetyl-Ala-Arg methyl ester
8.6
acetyl-Ala-Arg-Ala-NH2
-
pH 8.0, 25C
-
0.222
acetyl-Ala-Leu-Arg-p-nitroanilide
-
pH 8.5, 37C
0.035
acetyl-Ala-Phe-Arg methyl ester
-
pH 8.0, 25C
-
0.17
acetyl-Ala-Phe-Arg-Ala
-
pH 8.0, 25C
-
0.11
acetyl-Ala-Phe-Arg-p-nitroanilide
-
pH 8.0, 25C
0.571
acetyl-Ala-Thr-Arg-p-nitroanilide
-
pH 8.5, 37C
4.1
acetyl-Arg methyl ester
-
pH 8.0, 25C
16
acetyl-Arg p-nitroanilide
-
pH 8.0, 25C
14
acetyl-Arg-Ala-NH2
-
pH 8.0, 25C
2.5
acetyl-D-Ala-Arg methyl ester
-
pH 9.0, 25C
0.95
acetyl-D-Phe-Arg methyl ester
-
pH 9.0, 25C
0.849
acetyl-Glu-Leu-Arg-p-nitroanilide
-
pH 8.5, 37C
0.291
acetyl-Glu-Lys-Arg-p-nitroanilide
-
pH 8.5, 37C
0.765
acetyl-Glu-Phe-Arg-p-nitroanilide
-
pH 8.5, 37C
0.929
acetyl-Glu-Thr-Arg-p-nitroanilide
-
pH 8.5, 37C
0.77
acetyl-Gly-Arg methyl ester
-
pH 9.0, 25C
26
acetyl-Gly-Arg-Ser-Val-Gln
-
pH 9.0, 25C
0.199
acetyl-Ile-Arg methyl ester
-
pH 9.0, 25C
0.0249
acetyl-Leu-Arg methyl ester
-
pH 9.0, 25C
0.182
acetyl-Met-Arg methyl ester
-
pH 9.0, 25C
0.14
acetyl-Phe-Arg ethyl ester
-
-
0.0244 - 0.0311
acetyl-Phe-Arg methyl ester
0.27
acetyl-Phe-Arg-Ala-NH2
-
pH 8.0, 25C
0.15
acetyl-Phe-Arg-p-nitroanilide
-
pH 8.0, 25C
0.21
acetyl-Phe-Arg-Ser-NH2
-
pH 9.0, 25C
0.31
acetyl-Phe-Arg-Ser-Val-Gln
-
pH 9.0, 25C
0.04
acetyl-Phe-Arg-Ser-Val-NH2
-
pH 9.0, 25C
0.153
acetyl-Pro-Arg methyl ester
-
pH 9.0, 25C
0.089
acetyl-Pro-Phe-Arg methyl ester
-
pH 8.0, 25C
0.21
acetyl-Pro-Phe-Arg-Ala
-
pH 8.0, 25C
0.087
acetyl-Pro-Phe-Arg-p-nitroanilide
-
pH 8.0, 25C
0.027
acetyl-Trp-Arg methyl ester
-
pH 9.0, 25C
0.132
acetyl-Tyr-Arg methyl ester
-
pH 9.0, 25C
0.283
acetyl-Val-Arg methyl ester
-
pH 9.0, 25C
0.02
Ala-Arg-Arg-4-methylcoumarin 7-amide
0.45
benzoyl-Pro-Phe-Arg-p-nitroanilide
-
pH 8.0, 37C
0.243
benzyloxycarbonyl-Arg methyl ester
-
pH 8.0, 25C
1.25
benzyloxycarbonyl-Arg-Ala-NH2
-
pH 8.0, 25C
0.327
benzyloxycarbonyl-Arg-p-nitroanilide
-
pH 8.0, 25C
0.209
benzyloxycarbonyl-Pro-Gly-Arg methyl ester
-
-
0.0114
benzyloxycarbonyl-Pro-Phe-Arg methyl ester
-
pH 9.0, 25C
61.1
citrated bovine plasma
-
pH 7.8, 37C
-
50.8
citrated dog plasma
-
pH 7.8, 37C
-
0.00018 - 0.0031
D-Pro-Phe-Arg-4-methylcoumarin-7-amide
0.001 - 0.07
D-Pro-Phe-Phe-4-methylcoumarin-7-amide
0.0183 - 28.4
D-Val-Leu-Arg-p-nitroanilide
0.528 - 0.868
D-Val-Leu-Lys-p-nitroanilide
0.12 - 58.8