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EC Tree
The taxonomic range for the selected organisms is: Xenopus laevis The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
preferential cleavage at Gly-Ser-Arg373-/- of glycoprotein gp43 in Xenopus laevis coelemic egg envelope to yield gp41
Synonyms
alpha-fetoprotein, oviductin, ovochymase, oviductin-1, ovochymase 2, oviductin-i, oviductal protease, gp43 processing protease,
more
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Gp43 processing protease
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gp43 + H2O
gp41 + ?
oviductin alone is the oviductal factor responsible for converting the egg envelope to a sperm-penetrable form, via an increase in sperm binding. gp43 processing is the only requirement for envelope conversion
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-
?
Nalpha-tert-butoxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumarin-7-amide + H2O
Nalpha-tert-butoxycarbonyl-Leu-Ser-Thr-Arg + 7-amino-4-methylcoumarin
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14% of the activity with Nalpha-tert-butoxycarbonyl-Phe-Ser-Arg-4-methylcoumarin-7-amide
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?
Nalpha-tert-butoxycarbonyl-Leu-Thr-Arg-4-methylcoumarin-7-amide + H2O
Nalpha-tert-butoxycarbonyl-Leu-Thr-Arg + 7-amino-4-methylcoumarin
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12% of the activity with Nalpha-tert-butoxycarbonyl-Phe-Ser-Arg-4-methylcoumarin-7-amide
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-
?
Nalpha-tert-butoxycarbonyl-Phe-Ser-Arg-4-methylcoumarin-7-amide + H2O
Nalpha-tert-butoxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
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?
Nalpha-tert-butoxycarbonylphenylalanylserylarginyl-7-amido-4-methylcoumarin + H2O
Nalpha-tert-butoxycarbonylphenylalanylserylarginine + 7-amino-4-methylcoumarin
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?
Pro-Phe-Arg-4-methylcoumarin-7-amide + H2O
Pro-Phe-Arg + 7-amino-4-methylcoumarin
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9% of the activity with Nalpha-tert-butoxycarbonyl-Phe-Ser-Arg-4-methylcoumarin-7-amide
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?
Pyr-Gly-Arg-4-methylcoumarin-7-amide + H2O
Pyr-Gly-Arg + 7-amino-4-methylcoumarin
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9% of the activity with Nalpha-tert-butoxycarbonyl-Phe-Ser-Arg-4-methylcoumarin-7-amide
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?
gp43 + H2O
gp41 + ?
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preferential cleavage at Gly-Ser-Arg373-/-glycoprotein gp43 in Xenopus laevis coelemic egg envelope to yield gp41. Processing of gp43 causes physical changes in the egg envelope which occurs during egg transit through the oxiduct
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-
?
gp43 + H2O
gp41 + ?
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preferential cleavage at Gly-Ser-Arg373-/-glycoprotein gp43 in Xenopus laevis coelemic egg envelope to yield gp41
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
gp43 + H2O
gp41 + ?
oviductin alone is the oviductal factor responsible for converting the egg envelope to a sperm-penetrable form, via an increase in sperm binding. gp43 processing is the only requirement for envelope conversion
-
-
?
gp43 + H2O
gp41 + ?
-
preferential cleavage at Gly-Ser-Arg373-/-glycoprotein gp43 in Xenopus laevis coelemic egg envelope to yield gp41. Processing of gp43 causes physical changes in the egg envelope which occurs during egg transit through the oxiduct
-
-
?
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Ca2+
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38% stimulation at 10 mM. 11% stimulation at 100 mM
KCl
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maximal activity in 200-400 mM solution of monovalent salts is 200% of that in absence of added salt
LiCl
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maximal activity in 200-400 mM solution of monovalent salts is 200% of that in absence of added salt
NaCl
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maximal activity in 200-400 mM solution of monovalent salts is 200% of that in absence of added salt
NaF
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maximal activity in 200-400 mM solution of monovalent salts is 200% of that in absence of added salt
NaNO3
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maximal activity in 200-400 mM solution of monovalent salts is 200% of that in absence of added salt
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antipain
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0.05 mM, 35% inhibition
Aprotinin
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0.05 mM, 97% inhibition
diisopropyl fluorophosphate
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0.05 mM, 37% inhibition. Rapid and irreverible
EDTA
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0.05 mM, 90% inhibition
EGTA
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0.05 mM, 94% inhibition
guanidine hydrochloride
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competitive
leupeptin
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0.05 mM, 55% inhibition
p-aminobenzamidine
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competitive
Soybean trypsin inhibitor
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0.0025 mM, 84% inhibition
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additional information
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no inhibition by iodoacetamide, E-64, pepstatin or 1,10-phenanthroline
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0.058
Nalpha-tert-butoxycarbonylphenylalanylserylarginyl-7-amido-4-methylcoumarin
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3.8
Nalpha-tert-butoxycarbonylphenylalanylserylarginyl-7-amido-4-methylcoumarin
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0.0075
guanidine hydrochloride
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0.0041
p-aminobenzamidine
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-
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8
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Nalpha-tert-butoxycarbonylphenylalanylserylarginyl-4-methylcoumarin-7-amide
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7.5 - 8.5
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more than 90% of maximal activity at pH 7.5 and at pH 8.5, less than 30% of maximal activity below pH 6.5
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Uniprot
brenda
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brenda
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secretory granules
brenda
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oviductal
brenda
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OVCH2_XENLA
1004
0
110612
Swiss-Prot
Secretory Pathway (Reliability: 2 )
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66000
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x * 66000, SDS-PAGE under both nonreducing and reducing conditions
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?
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x * 66000, SDS-PAGE under both nonreducing and reducing conditions
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proteolytic modification
protease is translated as the N terminus of an unusual mosaic protein. It is proposed that during post-translational proteolytic processing of the mosaic oviductin glycoprotein, the processed N-terminal protease domain is released coupled to two C-terminal CUB domains and constitutes the enzymatically active protease molecule
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relatively stable to rapid freezing in the presence of 20% glycerol. 90% of the activity remains after each freeze-thaw cycle
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Hardy, D.M.; Hedrick, J.L.
Oviductin. Purification and properties of the oviductal protease that processes the molecular weight 43000 glycoprotein of the Xenopus laevis egg envelope
Biochemistry
31
4466-4472
1992
Xenopus laevis
brenda
Lindsay, L.L.; Wieduwilt, M.J.; Hedrick, J.L.
Oviductin, the Xenopus laevis oviductal protease that processes egg envelope glycoprotein gp43, increases sperm binding to envelopes, and is translated as part of an unusual mosaic protein composed of two protease and several CUB domains
Biol. Reprod.
60
989-995
1999
Xenopus laevis (P79953), Xenopus laevis
brenda